RESUMEN
The acid-base and Zn sorption properties of three bacteria, Cupriavidus metallidurans CH34, Pseudomonas putida ATCC12633, and Escherichia coli K12DH5alpha, were investigated through an original combination of extended X-ray absorption fine structure (EXAFS) spectroscopy and equilibrium titration studies. Acid-base titration curves of the three strains were fitted with a model accounting for three conceptual reactive sites: an acidic (carboxyl and/or phosphodiester), a neutral (phosphomonoester), and a basic (amine and/or hydroxyl) group. Calculated proton and Zn equilibrium constants and site densities compare with literature data. The nature of Zn binding sites was studied by EXAFS spectroscopy. Phosphoester, carboxyl, and unexpectedly sulfhydryl ligands were identified. Their proportions depended on Zn loading and bacterial strain and were consistent with the titration results. These findings were compared to the structure and site density of the major cell wall components. It appeared that the cumulated theoretical site density of these structures (<2 Zn nm(-2)) was much lower than the total site density of the investigated strains (16-56 Zn nm(-2)). These results suggest a dominant role of extracellular polymeric substances in Zn retention processes, although Zn binding to inner cell components cannot be excluded.
Asunto(s)
Bacterias Gramnegativas/química , Zinc/química , Adsorción , Aminas/química , Burkholderiaceae/química , Ácidos Carboxílicos/química , Escherichia coli/química , Ésteres/química , Concentración de Iones de Hidrógeno , Ligandos , Modelos Químicos , Protones , Pseudomonas putida/química , Espectrometría por Rayos X/métodos , Compuestos de Sulfhidrilo/química , Volumetría/métodosRESUMEN
In infiltration basins, such as in any kind of porous media, bacteria may form biofilms. When conditions induce destabilization of this biofilm, resulting colloids are transported by the hydraulic flow. Some studies have focused on the role played by these bacterial colloids in pollutants transport in soil. This study deals with the ability of Pseudomonas putida to retain zinc and investigates the facilitated transport of this metal. Batch and columns experiments have been carried out. Bacteria display a great affinity for zinc in batch experiments and facilitated transport have been highlighted in a small extent, for the moment. A scenario of stabilization/destabilization of the biofilm has been designed and may be employed for further investigations.