RESUMEN
A distinct Russian Mammalian orthorubulavirus 5 (PIV5) was detected in cell culture exhibiting cytopathic effect and hypothesized to be contaminated by a scientist with respiratory symptoms. The identification of the divergent strain indicated a lack of knowledge on the diversity of PIV5 strains and calls for surveillance of global PIV5 strains.
Asunto(s)
Virus de la Parainfluenza 5 , Células Vero/virología , Animales , Línea Celular , Chlorocebus aethiops , Efecto Citopatogénico Viral , Genoma Viral , Humanos , Mamíferos/virología , Virus de la Parainfluenza 5/clasificación , Virus de la Parainfluenza 5/genética , Virus de la Parainfluenza 5/aislamiento & purificación , Filogenia , Infecciones por Rubulavirus/virología , Federación de Rusia , Secuenciación Completa del GenomaRESUMEN
An outbreak of enteric disease of unknown etiology with 60% morbidity and 8% mortality in weaning piglets occurred in November 2015 on a farm in Buryat Republic, Russia. Metagenomic sequencing revealed the presence of rotavirus B in feces from diseased piglets while no other pathogens were identified. Clinical disease was reproduced in experimentally infected piglets, yielding the 11 RVB gene segments for strain Buryat15, with an RVB genotype constellation of G12-P[4]-I13-R4-C4-M4-A8-N10-T4-E4-H7. This genotype constellation has also been identified in the United States. While the Buryat15 VP7 protein lacked unique amino acid differences in the predicted neutralizing epitopes compared to the previously published swine RVB G12 strains, this report of RVB in Russian swine increases our epidemiological knowledge on the global prevalence and genetic diversity of RVB.
