Applied potential assisted biodegradation of amoxicillin (AMX) using bacterial consortium isolated from a waste dump site.

Antibiotics have revolutionized modern day living with their ability to effectively treat infectious diseases in humans and animals. However, the release of antibiotic compounds into the environment has led to toxic consequences. To reduce this environmental impact, it is important to employ an inexpensive and rational technology to reduce the amount of antibiotics released into the ecosystem. This study aims to explore the potential of using a bio-electrochemical system (BES) to remove Amoxicillin (AMX) from artificially contaminated soil using a microbial consortium and pure culture isolates. Under desired conditions, including an initial AMX concentration of 150 mg/L, 5 mg/L tryptone as the nitrogen source, pH of 7, temperature of 29 °C, an applied potential of 0.8 V, and an inoculum dose of 1% w/v, the BES showed a maximum degradation of 97.9% of AMX with the microbial consortium (HP03, HP09, and HP10). High performance liquid chromatography-mass spectrometry was used to analyse the intermediates formed during the degradation process, and the pathway elucidated revealed complete degradation of AMX. Phytotoxicity studies and degradation efficiency against multiple antibiotics confirmed the environmental significance of the BES with microbial consortium. Overall, this study highlights the potential of BES as a cost-effective and efficient method for reducing the release of antibiotics into the environment and provides valuable insights into the mechanisms and pathways of antibiotic degradation.

Detoxification of p-nitrophenol (PNP) using Enterococcus gallinarum JT-02 isolated from animal farm waste sludge.

Enterococcus gallinarum (JT-02) isolated and identified from the animal farm waste sludge was found to be capable of biodegrading p-nitrophenol (PNP), an organic compound used to manufacture drugs, fungicides, insecticides, dyes, and to darken leather. The intention of this study was to optimize the biodegradation by finding the optimal conditions for the specific strain through single-factor experiments. The bacterial strain was grown in Luria Bertani broth and various parameters were optimized to achieve the prime settings for the p-nitrophenol (PNP) biodegradation. The results indicated that the best setups for the biodegradation by the strain JT-02 was 100 mg/L of PNP; pH 7; 30 °C; 150 rpm in a shaker incubator and 3% (v/v) of inoculum dose. Once the optimal conditions were found, the bacteria were capable of degrading p-nitrophenol (98.21%) in 4 days. Intermediates produced during PNP biodegradation were identified using High Performance Liquid Chromatography (HPLC) analysis and the biodegradation pathway was elucidated. Phytotoxicity studies were carried out with Vigna radiata seeds to confirm the applicability and efficiency of PNP biodegradation.

A hybrid technique for sulfamethoxazole (SFM) removal using Enterobacter hormaechei HaG-7: Bio-electrokinetic degradation, pathway and toxicity.

Prolonged exposure to antibiotics would likely favor the development of antibiotic resistance and their gene transfer among bacterial communities that are responsible for enriched antibiotic resistant microbes. Sulfamethoxazole (SFM) is a commonly used antibiotic that is released into the environment through human and animal wastes. Improper degradation of SFM poses severe threats to mankind and all life forms. The present study aims in analyzing the process and the probability of utilizing bio-electrokinetic degradation for elimination of SFM from artificially contaminated soil employing Enterobacter hormaechei HaG-7. The desired optimal conditions for SFM degradation (∼98%) were observed at SFM initial concentration (100 mg/L) with an inoculum dose (1% v/v) and applied potential voltage (1.5 V) at pH (7). The results indicated efficient and complete degradation of SFM when compared with the conventional biodegradation.

Synthesis of quercetin functionalized wurtzite type zinc oxide nanoparticles and their potential to regulate intrinsic apoptosis signaling pathway in human metastatic ovarian cancer.

In the present study, the wurtzite (WZ) type of zinc oxide (ZnO) nanoparticles were synthesized and functionalized with quercetin (ZnO@Quercetin) for the treatment of ovarian cancer. Initially, the chemical synthesis of ZnO nanoparticles was confirmed with DRS UV-vis spectroscopy and the bandgap of the ZnO revealed that the WZ type of nanoparticles. The electron microscopy analysis showed the hexagonal shape, monocrystalline nature of nanoparticles with an average size of 20-25 nm and the SAED pattern showed the interplanar planes for WZ type nanoparticles. XRD analysis revealed the presence of strong peaks corresponding to ZnO nanoparticles and the Raman spectroscopic analysis showed the characteristic peaks at E2 (high) and E1 vibrational mode for WZ type of ZnO nanoparticles. The in vitro cytotoxic activity of ZnO@Quercetin nanoparticles showed the excellent activity by generating intercellular oxidative stress and depolarization of mitochondrial membrane potential against human ovarian cancer cells. The dual-staining assay showed that the ZnO@Quercetin induces late apoptosis through activation of the intrinsic apoptosis signaling pathway in PA-1 cells. Together, the present study indicates the ZnO@Quercetin nanoparticles can be used for the treatment of human metastatic ovarian cancer.

Highly efficient visible light photocatalysis of Nix Zn1-x Fe2O4 (x= 0, 0.3, 0.7) nanoparticles: Diclofenac degradation mechanism and eco-toxicity.

Pharmaceuticals and personal care products occupy a predominant position with respect to both utility and release into the ecosystem, thereby contributing to environmental pollution at alarming rates. Of the several methods identified to minimize the concentration of PPCPs, nanomaterial based photocatalysis seems to be a potential alternative for it being highly economical and eco-friendly. In this study, we synthesized Nickel zinc ferrite (Ni-ZF) [Nix Zn1-x Fe2O4 (x = 0, 0.3, 0.7)] nanoparticles with an average diameter of ∼400 nm by a co-precipitation method towards diclofenac degradation. The composite showed greater degrees of crystallinity devoid of any impurities. Nearly complete DCF degradation (∼99%) was achieved after 50 min reaction time with the nanoparticles at pH 7 for an initial DCF concentration of 50 mg/L. The degradation process followed a pseudo first-order rate law with the rate constant of 0.1657 min- 1. Microbial toxicity and phytotoxicity studies demonstrated negligible toxicity imposed by the contaminated water treated with the prepared composite, suggesting it as a promising photocatalyst benefitting in all aspects.