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1.
Fish Physiol Biochem ; 49(3): 425-439, 2023 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-37074473

RESUMEN

The splendid alfonsino Beryx splendens is a commercially important deep-sea fish in East Asian countries. Because the wild stock of this species has been declining, there is an urgent need to develop aquaculture systems. In the present study, we investigated the long-chain polyunsaturated fatty acid (LC-PUFA) requirements of B. splendens, which are known as essential dietary components in many carnivorous marine fish species. The fatty acid profiles of the muscles, liver, and stomach contents of B. splendens suggested that it acquires substantial levels of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from its natural diet. The functional characterization of a fatty acid desaturase (Fads2) and three elongases (Elovl5, Elovl4a, and Elovl4b) from B. splendens confirmed their enzymatic capabilities in LC-PUFA biosynthesis. Fads2 showed Δ6 and Δ8 bifunctional desaturase activities. Elovl5 showed preferential elongase activities toward C18 and C20 PUFA substrates, whereas Elovl4a and Elovl4b showed activities toward various C18-22 substrates. Given that Fads2 showed no Δ5 desaturase activity and no other fads-like sequence was found in the B. splendens genome, EPA and arachidonic acid cannot be synthesized from C18 precursors; hence, they can be categorized as dietary essential fatty acids in B. splendens. EPA can be converted into DHA in B. splendens via the so-called Sprecher pathway. However, given that fads2 is only expressed in the brain, it is unlikely that the capacity of B. splendens to biosynthesize DHA from EPA can fulfill its physiological requirements. These results will be useful to researchers developing B. splendens aquaculture methods.


Asunto(s)
Proteínas de Peces , Peces , Animales , Elongasas de Ácidos Grasos/genética , Elongasas de Ácidos Grasos/metabolismo , Proteínas de Peces/metabolismo , Peces/metabolismo , Ácido Graso Desaturasas/genética , Ácidos Grasos Esenciales , Ácido Eicosapentaenoico , Ácidos Docosahexaenoicos , Dieta/veterinaria , Ácidos Grasos
2.
Chem Biodivers ; 14(9)2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28686351

RESUMEN

N-Acetyl-d-glucosamino-1,5-lactone 1 has been reported as a candidate component of the sex pheromone mixture of female blue crabs, Callinectes sapidus, since it is present in the urine of reproductive females and males detect it. Theoretically, 1 can convert to a 1,4-lactone isomer 2 or to the corresponding carboxylic acid, 2-acetamido-2-deoxygluconic acid 3 by hydrolysis in aqueous solution. In this study, we examined the biologically relevant state of equilibrium mixture of 1, 2, and 3 in crab urine using ESI-MS and NMR analyses. The ESI-MS analysis showed that the dominant form of solubilized synthetic 1 is lactone 1 and/or 2, immediately after solubilization in deuterated water, seawater, and phosphate buffer and gradually changing to carboxylic acid 3 which becomes most predominant in phosphate buffer. The NMR analysis showed that synthetic 1 converts to other forms in deuterated water and seawater, and reaches an equilibrium mixture of at least three forms within 24 h. In contrast, 1 converts to a single state of another form in deuterated water with 35 mm phosphate buffer pH 7.6 within 24 h, which is identical to the state in urine with or without phosphate buffer. Thus, we conclude that the molting biomarker sensed by male crabs is 3.


Asunto(s)
Acetamidas/análisis , Braquiuros/crecimiento & desarrollo , Gluconatos/análisis , Muda , Animales , Braquiuros/química , Femenino , Lactonas/análisis , Espectroscopía de Resonancia Magnética , Masculino , Atractivos Sexuales/análisis , Espectrometría de Masa por Ionización de Electrospray , Orina/química
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