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Background Fusobacterium nucleatum (F. nucleatum) has been increasingly linked to oral squamous cell carcinoma (OSCC), prompting this study to explore its presence using polymerase chain reaction (PCR) and evaluate its clinical significance. Methods In this pilot case-control study, 12 OSCC tissue samples and 12 non-cancerous oral mucosal tissue samples were analyzed. Total RNA extraction and complementary DNA (cDNA) synthesis were performed using Trizol-based methods, followed by PCR amplification and gel electrophoresis. The clinical characteristics of participants and PCR results were recorded. Results Among the OSCC tissue samples, three out of 12 tested positive for F. nucleatum, while none of the control samples showed its presence. The detection rate of F. nucleatum in OSCC was 25%. Gel analysis confirmed specific amplicon amplification, and ImageJ software enabled copy number quantification. Discussion Our findings support previous research indicating a potential association between F. nucleatum and OSCC. Understanding the etiological significance of F. nucleatum in OSCC has clinical implications, including early detection, risk stratification, and prognostication. However, the limited sample size and the need for further research to elucidate underlying mechanisms are acknowledged. Conclusion This pilot study provides initial evidence of F. nucleatum's presence in a subset of OSCC samples, supporting its potential association with oral cancer. Detecting F. nucleatum in OSCC tissues holds promise for future research and clinical applications as a diagnostic and prognostic biomarker. Understanding its role in oral carcinogenesis will facilitate the development of targeted therapeutic strategies. Larger studies are warranted to validate these findings and investigate the precise mechanisms involved.
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The relationship between periodontitis (or periodontal disease) with Alzheimer's disease has been reported by various primary sources in the past decade, but not with a solid secondary research statement. A systematic review and meta-analysis was conducted in accordance with the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and registered (Reference number: CRD42020185264) with PROSPERO (International prospective register for systematic reviews). A literature search was conducted on specific databases for suitable articles in English language. Out of 612 studies selected, 41 underwent full-text analysis; five studies were eligible for systematic review, and 3 for meta-analysis. Meta-analysis was performed with tests for sensitivity and statistical heterogeneity followed by calculation of summary effect measures in terms of odds ratio (OR) and 95% confidence interval (CI). The results of this review showed a significant association between periodontitis and Alzheimer's disease in the meta-analysis [OR 1.67 (1.21-2.32)].
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BACKGROUND: Recurrent aphthous stomatitis represents one of the most common oral pathoses with multifactorial etiology. Decrease in mucosal barrier resistance is believed to be one of the etiologies in its pathogenesis. This study was conducted to assess the oral mucosal integrity status by evaluating the salivary albumin level in patients with aphthous stomatitis. MATERIALS AND METHODS: Thirty patients diagnosed with aphthous stomatitis were selected as case group. Equal number of age- and sex-matched healthy individuals formed the control group. Salivary albumin level was estimated during active and quiescent stage of the disease in both case group and control group. RESULT: Mean salivary albumin level for the case group during the active and quiescent stage was 0.070 g/dL (SD = 0.037) and 0.004 g/dL (SD = 0.007) (SPSS, version 7.0), respectively, and that for the control group was 0.027 g/dL (SD =0.042). Statistically significant difference was found on comparison of the mean salivary albumin level between the case group during active and quiescent stage and the normal controls using Mann-Whitney U test. No statistically significant difference in salivary albumin level was seen between the quiescent stage in case group and the normal controls. CONCLUSION: Increase in salivary albumin level at the time of disease presentation could be attributed to the leakage of albumin through the ulcerated mucosa. Absence of significant elevation in the salivary albumin level after the resolution of the aphthous ulcer apparently indicates inherently a healthy mucosal barrier in majority of the patients.
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Granular cell ameloblastoma is a rare variant of ameloblastoma, which is histopathologically characterized by the presence of large eosinophilic granular cells within the ameloblastic follicle. Its accurate preoperative diagnosis is based upon clinical, radiological, and incisional biopsy findings. This article reports a case of granular cell ameloblastoma in a 65-year-old female, which on incisional biopsy showed the features suggestive of aneurysmal bone cyst. Furthermore, the influence of macroscopic presentation of the current lesion on its accurate preoperative incisional biopsy diagnosis is discussed in detail.
