A review on phytoremediation of mercury contaminated soils.

Mercury (Hg) and its compounds are one of the most dangerous environmental pollutants and Hg pollution exists in soils in different degrees over the world. Phytoremediation of Hg-contaminated soils has attracted increasing attention for the advantages of low investment, in-situ remediation, potential economic benefits and so on. Searching for the hyperaccumulator of Hg and its application in practice become a research hotspot. In this context, we review the current literatures that introduce various experimental plant species for accumulating Hg and aided techniques improving the phytoremediation of Hg-contaminated soils. Experimental plant species for accumulating Hg and accumulation or translocation factor of Hg are listed in detail. The translocation factor (TF) is greater than 1.0 for some plant species, however, the bioaccumulation factor (BAF) is greater than 1.0 for Axonopus compressus only. Plant species, soil properties, weather condition, and the bioavailability and heterogeneity of Hg in soils are the main factors affecting the phytoremediation of Hg-contaminated soils. Chemical accelerator kinds and promoting effect of chemical accelerators for accumulating and transferring Hg by various plant species are also discussed. Potassium iodide, compost, ammonium sulphate, ammonium thiosulfate, sodium sulfite, sodium thiosulfate, hydrochloric acid and sulfur fertilizer may be selected to promote the absorption of Hg by plants. The review introduces transgenic gene kinds and promoting effect of transgenic plants for accumulating and transferring Hg in detail. Some transgenic plants can accumulate more Hg than non-transgenic plants. The composition of rhizosphere microorganisms of remediation plants and the effect of rhizosphere microorganisms on the phytoremediation of Hg-contaminated soils are also introduced. Some rhizosphere microorganisms can increase the mobility of Hg in soils and are beneficial for the phytoremediation.

Transcriptional Changes in the Ovaries of Perch from Chernobyl.

Fish have been highly exposed to radiation in freshwater systems after the Chernobyl Nuclear Power Plant (NPP) accident in 1986 and in freshwater and marine systems after the more recent Fukushima NPP accident in 2011. In the years after the accident, the radioactivity levels rapidly declined due to radioactive decay and environmental processes, but chronic lower dose exposures persisted. To gain insights into the long-term effects of environmental low dose radiation on fish ovaries development, a high-throughput transcriptomic approach including a de novo assembly was applied to different gonad phenotypes of female perch: developed gonads from reference lakes, developed/irradiated from medium contaminated lake, and both developed/irradiated and undeveloped from more highly contaminated lakes. This is the most comprehensive analysis to date of the gene responses in wildlife reproductive system to radiation. Some gene responses that were modulated in irradiated gonads were found to be involved in biological processes including cell differentiation and proliferation (ggnb2, mod5, rergl), cytoskeleton organization (k1C18, mtpn), gonad development (nell2, tcp4), lipid metabolism (ldah, at11b, nltp), reproduction (cyb5, cyp17A, ovos), DNA damage repair (wdhd1, rad51, hus1), and epigenetic mechanisms (dmap1). Identification of these genes provides a better understanding of the underlying molecular mechanisms underpinning the development of the gonad phenotypes of wild perch and how fish may respond to chronic exposure to radiation in their natural environment, though causal attribution of gene responses remains unclear in the undeveloped gonads.

A review on control factors of pyrolysis technology for plants containing heavy metals.

The treatment of plants with heavy metals, whether they grow naturally in heavy metal contaminated soil or are used for remediation of heavy metal contaminated soil has attracted increasing attention. Pyrolysis is often used for the disposal of plants with heavy metals because it stabilizes heavy metals effectively and produces biochar. The resulting products of pyrolysis are in the form of solid components (char and ash), liquid components (bio-oil and tar), together with gas components (condensable and non-condensable vapor gas). The metal amount in the char or liquid and gaseous phases can be maximized or minimized via treating a plant feedstock containing heavy metals under different conditions. In addition, the potential risk of biochar produced from plants after pyrolysis becomes a research hotspot in the field of pyrolysis technology of plants containing heavy metals. Herein, we review current literatures that emphasize the influencing factors on the metal content in the biochar, liquid and gaseous phases, as well as the potential risk of biochar.

Chronic radiation exposure at Chernobyl shows no effect on genetic diversity in the freshwater crustacean, Asellus aquaticus thirty years on.

Analysis of genetic diversity represents a fundamental component of ecological risk assessments in contaminated environments. Many studies have assessed the genetic implications of chronic radiation exposure at Chernobyl, generally recording an elevated genetic diversity and mutation rate in rodents, plants, and birds inhabiting contaminated areas. Only limited studies have considered genetic diversity in aquatic biota at Chernobyl, despite the large number of freshwater systems where elevated dose rates will persist for many years. Consequently, the present study aimed to assess the effects of chronic radiation exposure on genetic diversity in the freshwater crustacean, Asellus aquaticus, using a genome-wide SNP approach (Genotyping-by-sequencing). It was hypothesized that genetic diversity in A. aquaticus would be positively correlated with dose rate. A. aquaticus was collected from six lakes in Belarus and the Ukraine ranging in dose rate from 0.064 to 27.1 µGy/hr. Genotyping-by-sequencing analysis was performed on 74 individuals. A significant relationship between geographical distance and genetic differentiation confirmed the Isolation-by-Distance model. Conversely, no significant relationship between dose rate and genetic differentiation suggested no effect of the contamination gradient on genetic differentiation between populations. No significant relationship between five measures of genetic diversity and dose rate was recorded, suggesting that radiation exposure has not significantly influenced genetic diversity in A. aquaticus at Chernobyl. This is the first study to adopt a genome-wide SNP approach to assess the impacts of environmental radiation exposure on biota. These findings are fundamental to understanding the long-term success of aquatic populations in contaminated environments at Chernobyl and Fukushima.

Effect of mixed solutions of heavy metal eluents on soil fertility and microorganisms.

This study analyzed the effect of heavy metal eluents (0.3 mol/L C6H8O7, 5 × 10-4 mol/L EDTA, and 0.01 mol/L Na2S2O3) on the content of organic matter, hydrolytic nitrogen, available phosphorus and potassium, and species composition of bacteria and fungi in vegetable soils. The obtained results documented that the treatment of the soil, consisting of shaking the sample with a mixture of eluents, significantly increased the content of organic matter, hydrolytic nitrogen, and available phosphorus and potassium. The mixed solutions of eluents increase the maximum available P in the soil by 279.3%, and hydrolytic N by 30.7%. The eluents affected, to a certain extent, the dominant species of microorganisms in the soil, but did not increase species richness and evenness in all soil samples.

Impact of Environmental Radiation on the Health and Reproductive Status of Fish from Chernobyl.

Aquatic organisms at Chernobyl have now been chronically exposed to environmental radiation for three decades. The biological effects of acute exposure to radiation are relatively well documented, but much less is known about the long-term effects of chronic exposure of organisms in their natural environment. Highly exposed fish in freshwater systems at Chernobyl showed morphological changes in their reproductive system in the years after the accident. However, the relatively limited scope of past studies did not allow robust conclusions to be drawn. Moreover, the level of the radiation dose at which significant effects on wildlife occur is still under debate. In the most comprehensive evaluation of the effects of chronic radiation on wild fish populations to date, the present study measures specific activities of 137Cs, 90Sr, and transuranium elements (238Pu, 239,240Pu, and 241Am), index conditions, distribution and size of oocytes, as well as environmental and biological confounding factors in two fish species perch ( Perca fluviatilis) and roach ( Rutilus rutilus) from seven lakes. In addition, relative species abundance was examined. The results showed that both fish species are, perhaps surprisingly, in good general physiological and reproductive health. Perch, however, appeared to be more sensitive to radiation than roach: in the most contaminated lakes, a delay of the maturation of the gonads and the presence of several undeveloped phenotypes were evident only for perch and not for roach.

Reproduction in the freshwater crustacean Asellus aquaticus along a gradient of radionuclide contamination at Chernobyl.

Nuclear accidents such as Chernobyl and Fukushima have led to contamination of the environment that will persist for many years. The consequences of chronic low-dose radiation exposure for non-human organisms inhabiting contaminated environments remain unclear. In radioecology, crustaceans are important model organisms for the development of environmental radioprotection. Previous laboratory studies have demonstrated deleterious effects of radiation exposure on crustacean reproduction. However, no studies have documented the effects of chronic radiation exposure on the reproduction of natural crustacean populations. Based on data from laboratory exposures, we hypothesised that populations of the freshwater isopod Asellus aquaticus exposed to radiation for thirty years at Chernobyl would display reduced reproductive output and altered timing of reproduction. To test this hypothesis, A. aquaticus was collected from six lakes at Chernobyl over two years with total dose rates ranging from 0.06-27.1µGy/h. No significant differences in the fecundity, mass of broods or proportion of reproducing female A. aquaticus were recorded. Significant differences in the body mass of gravid females were recorded suggesting different timings of reproduction, however this was not related to radiation contamination. No significant effect of a range of environmental parameters on A. aquaticus reproduction was recorded. Our data suggests current dose rates at Chernobyl are not causing discernible effects on the reproductive output of A. aquaticus. This study is the first to assess the effects of chronic low-dose radiation exposure on the reproductive output of an aquatic invertebrate at Chernobyl. These findings are consistent with proposed radiological protection benchmarks for the maintenance of wildlife populations and will assist in management of environments impacted by radiation.

Does Chernobyl-derived radiation impact the developmental stability of Asellus aquaticus 30years on?

Effects of long-term, environmentally relevant doses of radiation on biota remain unclear due to a lack of studies following chronic exposure in contaminated environments. The 1986 Chernobyl accident dispersed vast amounts of radioactivity into the environment which persists to date. Despite three decades of research, impacts of the incident on non-human organisms continues to be contested within the scientific literature. The present study assessed the impact of chronic radiation exposure from Chernobyl on the developmental stability of the model aquatic isopod, Asellus aquaticus using fluctuating asymmetry (FA) as an indicator. Fluctuating asymmetry, defined as random deviations from the expected perfect bilateral symmetry of an organism, has gained prominence as an indicator of developmental stability in ecotoxicology. Organisms were collected from six lakes along a gradient of radionuclide contamination in Belarus and the Ukraine. Calculated total dose rates ranged from 0.06-27.1µGy/h. Fluctuating asymmetry was assessed in four meristic and one metrical trait. Significant differences in levels of pooled asymmetry were recorded between sample sites independent of sex and specific trait measured. However, there was no correlation of asymmetry with radiation doses, suggesting that differences in asymmetry were not attributed to radionuclide contamination and were driven by elevated asymmetry at a single site. No correlation between FA and measured environmental parameters suggested a biotic factor driving observed FA differences. This study appears to be the first to record no evident increase in developmental stability of biota from the Chernobyl region. These findings will aid in understanding the response of organisms to chronic pollutant exposure and the long term effects of large scale nuclear incidents such as Chernobyl and Fukushima.