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Previous studies demonstrated that laboratory rats could visually receive emotional pain signals from conspecifics through pictorial stimuli. The present study examined whether a prior painful emotional experience of the receiver influenced the sensitivity of emotional expression recognition in laboratory rats. The experiment comprised four phases: the baseline preference test, pain manipulation test, post-manipulation preference test, and state anxiety test. In the baseline phase, the rats explored an apparatus comprising two boxes to which pictures of pain or neutral expressions of other conspecifics were attached. In the pain manipulation phase, each rat was allocated to one of three conditions: foot shock alone (pained-alone; PA), foot shock with other unfamiliar conspecifics (pained-with-other; PWO), or no foot shock (control). In the post-manipulation phase, the animals explored the apparatus in the same manner as they did in the baseline phase. Finally, an open-field test was used to measure state anxiety. These findings indicate that rats in the PWO group stayed longer per entry in a box with photographs depicting a neutral disposition than in a box with photographs depicting pain after manipulation. The results of the open-field test showed no significant differences between the groups, suggesting that the increased sensitivity to pain expression in other individuals due to pain experiences in social settings was not due to increased primary state anxiety. Furthermore, the results indicate that rats may use a combination of self-painful experiences and the states of other conspecifics to process the emotional signal of pain from other conspecifics. In addition, changes in the responses of rats to facial expressions in accordance with social experience suggest that the expression function of rats is not only used for emotional expressions but also for communication.
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HIV-1 Vif plays an essential role in viral replication by antagonizing anti-viral cellular restriction factors, a family of APOBEC3 proteins. We have previously shown that naturally-occurring single-nucleotide mutations in the SA1D2prox region, which surrounds the splicing acceptor 1 and splicing donor 2 sites of the HIV-1 genome, dramatically alter the Vif expression level, resulting in variants with low or excessive Vif expression. In this study, we investigated how these HIV-1 variants with poor replication ability adapt and evolve under the pressure of APOBEC3 proteins. Adapted clones obtained through adaptation experiments exhibited an altered replication ability and Vif expression level compared to each parental clone. While various mutations were present throughout the viral genome, all replication-competent adapted clones with altered Vif expression levels were found to bear them within SA1D2prox, without exception. Indeed, the mutations identified within SA1D2prox were responsible for changes in the Vif expression levels and altered the splicing pattern. Moreover, for samples collected from HIV-1-infected patients, we showed that the nucleotide sequences of SA1D2prox can be chronologically changed and concomitantly affect the Vif expression levels. Taken together, these results demonstrated the importance of the SA1D2prox nucleotide sequence for modulating the Vif expression level during HIV-1 replication and adaptation.
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Infecciones por VIH/genética , Sitios de Empalme de ARN/genética , Productos del Gen vif del Virus de la Inmunodeficiencia Humana/genética , Desaminasas APOBEC/metabolismo , Adaptación Fisiológica/genética , Antirretrovirales/uso terapéutico , Secuencia de Bases/genética , Linfocitos T CD4-Positivos/metabolismo , Linfocitos T CD4-Positivos/virología , ADN Viral/genética , Expresión Génica/genética , Regulación Viral de la Expresión Génica/genética , Genoma Viral/genética , Genómica/métodos , Células HEK293 , Infecciones por VIH/virología , VIH-1/genética , VIH-1/patogenicidad , Humanos , ARN Viral/genética , Replicación Viral/efectos de los fármacos , Replicación Viral/genética , Replicación Viral/fisiología , Productos del Gen vif del Virus de la Inmunodeficiencia Humana/metabolismoRESUMEN
Temperature is one of the major environmental factors that people are exposed to on a daily basis, often in conditions that do not afford control. It is known that heat and cold can influence a person's productivity and performance in simple tasks. With respect to social cognition, it has also been suggested that temperature impacts on relatively high-level forms of decision-making. For instance, previous research demonstrated that cold temperature promotes utilitarian judgment in a moral dilemma task. This effect could be due to psychological processing, when a cool temperature primes a set of internal representations (associated with "coldness"). Alternatively, the promotion of utilitarian judgment in cold conditions could be due to physiological interference from temperature, impeding on social cognition. Refuting both explanations of psychological or physiological processing, however, it has been suggested that there may be problems of reproducibility in the literature on temperature modulating complex or abstract information processing. To examine the role of temperature in moral decision-making, we conducted a series of experiments using ambient and haptic temperature with careful manipulation checks and modified task methodology. Experiment 1 manipulated room temperature with cool (21°C), control (24°C) and hot (27°C) conditions and found only a cool temperature effect, promoting utilitarian judgment as in the previous study. Experiment 2 manipulated the intensity of haptic temperature but failed to obtain the cool temperature effect. Experiments 3 and 4 examined the generalizability of the cool ambient temperature effect with another moral judgment task and with manipulation of exposure duration. However, again there were no cool temperature effects, suggesting a lack of reproducibility. Despite successful manipulations of temperature in all four experiments, as measured in body temperature and the participants' self-reported perception, we found no systematic influence of temperature on moral decision-making. A Bayesian meta-analysis of the four experiments showed that the overall data tended to provide strong support in favor of the null hypothesis. We propose that, at least in the range of temperatures from 21 to 27°C, the cool temperature effect in moral decision-making is not a robust phenomenon.
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Despite a growing number of studies suggesting that emotion words affect perceptual judgments of emotional stimuli, little is known about how emotion words affect perceptual memory for emotional faces. In Experiments 1 and 2 we tested how emotion words (compared with control words) affected participants' abilities to select a target emotional face from among distractor faces. Participants were generally more likely to false alarm to distractor emotional faces when primed with an emotion word congruent with the face (compared with a control word). Moreover, participants showed both decreased sensitivity (d') to discriminate between target and distractor faces, as well as altered response biases (c; more likely to answer "yes") when primed with an emotion word (compared with a control word). In Experiment 3 we showed that emotion words had more of an effect on perceptual memory judgments when the structural information in the target face was limited, as well as when participants were only able to categorize the face with a partially congruent emotion word. The overall results are consistent with the idea that emotion words affect the encoding of emotional faces in perceptual memory. (PsycINFO Database Record
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Emociones/fisiología , Cara/fisiología , Femenino , Humanos , Masculino , PercepciónRESUMEN
Recent studies have suggested that rodent behaviour is influenced by the behaviour of surrounding conspecifics (e.g. emotional contagion and prosocial behaviour). However, little is known about deferred imitation and complex observational learning in rats. The purpose of this study was to reveal whether rats can learn from another rat's experiences. In a maze, observer rats watched the foraging behaviour of other rats (demonstrators) and then foraged in turn. The results showed that demonstrators explored inefficiently, but observers explored more efficiently after observing inefficient exploration by the demonstrators. This observational learning probably involved the acquisition of an efficient strategy through spatial exploration.
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Though recent studies have shown that rodents express emotions with their face, whether emotional expression in rodents has a communicative function between conspecifics is still unclear. Here, we demonstrate the ability of visual recognition of emotional expressions in laboratory rats. We found that Long-Evans rats avoid images of pain expressions of conspecifics but not those of neutral expressions. The results indicate that rats use visual emotional signals from conspecifics to adjust their behaviour in an environment to avoid a potentially dangerous place. Therefore, emotional expression in rodents, rather than just a mere 'expression' of emotional states, might have a communicative function.
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Zajonc's drive theory postulates that arousal enhanced through the perception of the presence of other individuals plays a crucial role in social facilitation (Zajonc, 1965). Here, we conducted two experiments to examine whether the elevation of arousal through a stepping exercise performed in front of others as an exogenous factor causes social facilitation of a cognitive task in a condition where the presence of others does not elevate the arousal level. In the main experiment, as an "aftereffect of social stimulus," we manipulated the presence or absence of others and arousal enhancement before participants conducted the primary cognitive task. The results showed that the strongest social facilitation was induced by the combination of the perception of others and arousal enhancement. In a supplementary experiment, we manipulated these factors by adding the presence of another person during the task. The results showed that the effect of the presence of the other during the primary task is enough on its own to produce facilitation of task performance regardless of the arousal enhancement as an aftereffect of social stimulus. Our study therefore extends the framework of Zajonc's drive theory in that the combination of the perception of others and enhanced arousal as an "aftereffect" was found to induce social facilitation especially when participants did not experience the presence of others while conducting the primary task.
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In the current research, we extend past work on the effects of ambient darkness and threat to the domain of memory for expressive faces. In one study, we examined the effects of ambient darkness and individual differences in state anxiety on memory of unfamiliar expressive faces. Here, participants were seated in either a dark or light room and encoded a set of unfamiliar faces with angry, happy, and neutral facial expressions. A subsequent recognition task revealed an interactive effect of ambient darkness, anxiety, and target expression. Highly anxious participants in ambient darkness had worse memory for angry faces than did low-anxiety participants. On the other hand, the recognition performance for happy faces was affected neither by the darkness nor state anxiety. The results suggest not only that ambient darkness has its strongest effect on anxious perceivers, but also that person × situation effects should be considered in face recognition research.
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The International Commission on Radiological Protection recommends diagnostic reference levels (DRL) in each radiological examination for justification and optimization of patients' dose in medicine. The aim of our study was to propose the dose management system by utilizing dose information in diagnostic X-ray radiation dose structured report (Dose SR) in The Digital Imaging and Communications in Medicine to optimize radiation dose in institutions. Our dose management system is able to organize dose information obtained from various angiography systems and CTs. It is possible to provide this information to operators for justification and optimization of patient dose. Our system would be useful for the estimation of organ dose and could be used for the determination of local DRL (LDRL) for each radiological practice. In addition, the optimization became possible to compare LDRL with national DRL.
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Angiografía/normas , Sistemas de Administración de Bases de Datos , Dosis de Radiación , Protección Radiológica/normas , Sistemas de Información Radiológica , Bases de Datos Factuales , Humanos , Estándares de ReferenciaRESUMEN
We report data from an experiment that investigated the influence of gaze direction and facial expression on face memory. Participants were shown a set of unfamiliar faces with either happy or angry facial expressions, which were either gazing straight ahead or had their gaze averted to one side. Memory for faces that were initially shown with angry expressions was found to be poorer when these faces had averted as opposed to direct gaze, whereas memory for individuals shown with happy faces was unaffected by gaze direction. We suggest that memory for another individual's face partly depends on an evaluation of the behavioural intention of that individual.
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Movimientos Oculares/fisiología , Expresión Facial , Reconocimiento en Psicología/fisiología , Adulto , Emociones/fisiología , Femenino , Humanos , Masculino , Estimulación Luminosa , Desempeño Psicomotor/fisiología , Tiempo de Reacción/fisiologíaRESUMEN
Previous work has shown that individuals agree across cultures on the traits that they infer from faces. Previous work has also shown that inferences from faces can be predictive of important outcomes within cultures. The current research merges these two lines of work. In a series of cross-cultural studies, the authors asked American and Japanese participants to provide naïve inferences of traits from the faces of U.S. political candidates (Studies 1 and 3) and Japanese political candidates (Studies 2 and 4). Perceivers showed high agreement in their ratings of the faces, regardless of culture, and both sets of judgments were predictive of an important ecological outcome (the percentage of votes that each candidate received in the actual election). The traits predicting electoral success differed, however, depending on the targets' culture. Thus, when American and Japanese participants were asked to provide explicit inferences of how likely each candidate would be to win an election (Studies 3-4), judgments were predictive only for same-culture candidates. Attempts to infer the electoral success for the foreign culture showed evidence of self-projection. Therefore, perceivers can reliably infer predictive information from faces but require knowledge about the target's culture to make these predictions accurately.
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Comparación Transcultural , Cara , Política , Femenino , Humanos , Japón , Juicio , Liderazgo , Masculino , Personalidad , Autoimagen , Percepción Social , Estados UnidosRESUMEN
Two factors that influence the external rotation angle of the femoral rotational axis in total knee arthroplasty (TKA) were assessed in 40 medial osteoarthritic knees with varus deformity. First, the anatomic configuration of the femur was assessed using standardized radiographs of the patients' lower extremities before TKA. Second, the degree of medial soft tissue release was assessed during TKA. The radiographs showed that the characteristics of the femur were lateral bowing of the shaft and external rotation of the condyle in the coronal plane. Therefore, when the distal femur is cut perpendicular to the mechanical axis, the cut surface may be in too much of a valgus position. Furthermore, some degree of medial soft tissue release was necessary in all knees. Medial soft tissue release rotates the femur externally in extension in the coronal plane, and it rotates the femur externally around the femoral axis in flexion relative to the tibia. A distal femoral cut in too much of a valgus position and medial soft tissue release induces varus instability in flexion in knees with lateral bowing of the femoral shaft. Anatomic variation such as femoral bowing should be considered when a navigation system is used for TKA because the navigation system shows only the mechanical axis.
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Fémur/anomalías , Fémur/cirugía , Osteoartritis de la Rodilla/fisiopatología , Osteoartritis de la Rodilla/cirugía , Rango del Movimiento Articular/fisiología , Cirugía Asistida por Computador , Anciano , Anciano de 80 o más Años , Artroplastia de Reemplazo de Rodilla , Femenino , Fémur/diagnóstico por imagen , Humanos , Inestabilidad de la Articulación/etiología , Ligamento Colateral Medial de la Rodilla/cirugía , Persona de Mediana Edad , Osteoartritis de la Rodilla/diagnóstico por imagen , RadiografíaRESUMEN
During total knee arthroplasty in fifty consecutive cases, the distal femur and proximal tibia were initially cut. After posterior cruciate ligament excision, the femorotibial joint was expanded by a Tensor/balancer device with 30 inch-pounds of torque (in.lbs) both in extension and flexion, and ligament balancing was obtained in full extension. Then the knee was flexed at 90 degrees, and the femoral rotational axis was decided so that the axis was parallel to the tibial cut surface and the joint gap was the same between extension and flexion. The relationship between the distance of the joint gap expanded by a Tensor/balancer device with 30 in.lbs and the size of the bearing insert was assessed. The results showed that a 24 or 25-mm joint gap expanded by a Tensor/balancer device in full extension was optimal for a 10-mm bearing insert. Therefore, if the resection level of the tibia is set 24 or 25 mm from the femoral cut surface, a 10-mm bearing insert can be used. In 49 cases, the size of the femoral component was one size (4 mm) larger than that predicted based on the bony structure shown in the radiographs of the knee. With this procedure, ligament balancing and optimal joint gap both in extension and flexion can be obtained based on the predicted bearing insert in the knee.
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Artroplastia de Reemplazo de Rodilla/métodos , Humanos , Ligamentos Articulares/fisiologíaRESUMEN
Fracture of the os peroneum is relatively rare, and a stress fracture of the os peroneum is even rarer. We report a case of stress fracture of the os peroneum in a 38-year-old laborer who had been working in a crouching position. X-rays showed a multipartite os peroneum. Bone scintigram showed uptake at the os peroneum. The histology of the excised os peroneum revealed various stages of fracture healing. Excision of the fractured os peroneum and reconstruction of the peroneus longus tendon by end-to-end suture resulted in an excellent outcome.