Appendage abnormalities in spiders induced by an alternating temperature protocol in the context of recent advances in molecular spider embryology.

In the literature there are numerous reports of developmental deformities in arthropods collected in their natural habitat. Since such teratogenically affected individuals are found purely by chance, the causes of their defects are unknown. Numerous potential physical, mechanical, chemical, and biological teratogens have been considered and tested in the laboratory. Thermal shocks, frequently used in teratological research on the spider Eratigena atrica, have led to deformities on both the prosoma and the opisthosoma. In the 2020/2021 breeding season, by applying alternating temperatures (14 °C and 32 °C, changed every 12 h) for the first 10 days of embryonic development, we obtained 212 postembryos (out of 3,007) with the following anomalies: oligomely, heterosymely, bicephaly, schistomely, symely, polymely, complex anomalies, and others. From these we selected six spiders with defects on the prosoma and two with short appendages on the pedicel for further consideration. The latter cases seem particularly interesting because appendages do not normally develop on this body part, viewed as the first segment of the opisthosoma, and appear to represent examples of atavism. In view of the ongoing development of molecular techniques and recent research on developmental mechanisms in spiders, we believe the observed phenotypes may result, at least in part, from the erroneous suppression or expression of segmentation or appendage patterning genes. We consider "knockdown" experiments described in the literature as a means for generating hypotheses about the sources of temperature-induced body abnormalities in E. atrica.

Teratological changes in postembryos of Eratigena atrica obtained by the application of alternating temperatures on spider embryos.

Spider embryonic development depends on several factors, including temperature. Under optimum thermal conditions embryogenesis proceeds undisturbed and embryo mortality is low. On the other hand, dramatic shifts in incubation temperature may cause a range of developmental defects in embryos. It has been confirmed in numerous laboratory experiments that abrupt temperature changes can be a powerful teratogenic factor. Changes in the external structure are frequently reflected in the internal anatomy, and above all, in the central nervous system. In the present teratological study, by exposing spider embryos to the temperatures of 14 °C and 32 °C, changed every 12 hours for the first 10 days of their development, we obtained 74 postembryos of Eratigena atrica with body deformities such as oligomely, heterosymely, schistomely, bicephaly, complex anomalies and others. We selected six spiders to describe and analyze their morphological changes. In one case, that of a spider affected by polymely (the presence of a supernumerary appendage) combined with heterosymely (the fusion of walking legs), we also focused on the structure of the central nervous system. The analysis indicated that this complex anomaly was accompanied by only one change in the central nervous system: the presence of a supernumerary neuropil. Since no fusion of walking leg neuropils was observed, it was concluded that, in this instance, there was no relationship between the fusion of legs and the structure of the central nervous system.

Structure of the alimentary tract in the Atlantic mudskipper Periophthalmus barbarus (Gobiidae: Oxudercinae): anatomical, histological and ultrastructural studies.

The alimentary tract of oxudercine gobies is characterized by a lack of an anatomically distinct stomach, owing to which they are classified as stomachless. Since the environment, food requirements, and feeding habits have a significant impact on the anatomy of the alimentary tract of fish, it was assumed that predominantly carnivorous, semi-terrestrial mudskippers would have a stomach. In order to verify this hypothesis, anatomical, histological, histochemical and ultrastructural analysis of the alimentary tract of the Atlantic mudskipper Periophthalmus barbarus was performed. The results revealed that despite a lack of clear anatomical distinction within the alimentary tract, there were four well-distinguished sections visible at the histological level: oesophagus, stomach, intestine, and rectum. The division was enhanced by the presence of a pyloric sphincter and an ileorectal valve. The stomach contained tubular glands composed of oxynticopeptic cells. Gland cells had pepsinogen granules and a well-developed tubulovesicular network of smooth membranes, which indicates the secretion of gastric juice. The presence of neutral mucus in the apical region of surface epithelial cells as protection against hydrochloric acid as well as the presence of active pepsin also confirm gastric function. However, low pepsin activity seems to implies low protein digestion. The results of this study indicate that the Atlantic mudskipper P. barbarus has a functional stomach.

The effect of temperature and light on embryogenesis and post-embryogenesis of the spider Eratigena atrica (Araneae, Agelenidae).

Embryogenesis and post-embryogenesis of spiders depend on several environmental factors including light and temperature. This study was aimed at evaluating the impact of different thermal and lighting conditions on embryonic and early post-embryonic development of Eratigena atrica. Embryos, larvae, nymphs I and II were incubated at constant temperatures of 12, 22, 25 and 32°C under three different light regimes: light, dark, light/dark. Extreme temperatures (12 and 32°C) significantly increased mortality of embryos (to 100%) and nymphs II, whereas larvae and nymphs I suffered reduced survival only at the lowest temperature. Moreover, the lowest temperature reduced the development rate of all stages. The impact of light conditions was less pronounced and more variable: constant light reduced the survival of nymphs I at lower temperatures, but increased that of larvae. Moreover, light increased the time of embryonic development and duration of nymphal stages, particularly at lower temperatures (12-22°C). Thus, the most optimal locations for spiders seem to be dark (though except larval stage) and warm (25°C) sites, where their development is fastest and mortality lowest.

The allometry of the arcuate body in the postembryonic development of the giant house spider Eratigena atrica.

The brain of arachnids contains a special neuropil area called the arcuate body (AB), whose function has been widely discussed. Its growth and proportion in the brain volume during postembryogenesis have been investigated only in several spider species. Our allometric study is aimed at determining to what extent the development of the AB in Eratigena atrica, a spider with unique biology and behaviour, is similar to the development of this body in other species. We put forward a hypothesis of allometric growth of this body in relation to the volume of the central nervous system (CNS) and its neuropil as well as in relation to the volume of the brain and its neuropil. The analysis of paraffin embedded, H + E stained histological preparations confirmed our hypothesis. The AB developed more slowly than the CNS and the neuropil of both the brain and the CNS. In contrast, it exhibited positive allometry in relation to the volume of the brain. This body increased more than nine times within the postembryonic development. Its proportion in the brain volume varied; the lowest was recorded in larvae and nymphs I; then, it increased in nymphs VI and decreased to 2.93% in nymphs X. We conclude that in Eratigena atrica, the AB develops differently that in orb-weaver and wandering spiders. There is no universal model of the AB development, although in adult spiders, regardless of their behaviour, the proportion of this area in the brain volume is similar.

Morphology and the central nervous system of Eratigena atrica affected by a complex anomaly in the anterior part of the prosoma.

Spider embryogenesis is affected by a range of environmental factors. Any sudden, drastic change in the environment may impair spider development, leading to various body deformities. In the present study, we analyze changes in the morphology and structure of the central nervous system of an Eratigena atrica larva, obtained in a teratological experiment in which embryos were exposed to alternating temperatures of 14 and 32 °C for the first 10 days. The studied larva had three pedipalps on the right side of the prosoma (polymely), two of which were fused along their entire length (total heterosymely). In addition, there was a short, club-shaped stump between the pedipalps. Histological analysis confirmed major changes in the structure of the subesophageal ganglion, i.e., the fusion of all three ganglia of pedipalps.

The allometry of the central nervous system during the postembryonic development of the spider Eratigena atrica.

During ontogenesis, the size of a spider body, tissues and organs increases dramatically. The aim of the study was to estimate changes in the central nervous system of postembryonic stages of Eratigena atrica and compare them with the literature data on species differing in behavioural traits. Allometric analysis involved evaluation of histological slides embedded in paraffin and stained with hematoxylin and eosin. The reduced major axis regression (RMA) was applied to find allometric relationships between the volumes of the particular parts of the body. All the measured parts of the central nervous system (CNS) were negatively allometrically related to the volume of the prosoma, showing that the increment of the CNS was lower than that of the entire body. The growth of the brain was negatively allometrically related to the growth of the CNS but the increment of the subesophageal ganglion was greater than that of the CNS, exhibiting a positive allometry. Within both these structures, the increase in neuropil volume was greater than the growth of the cortex (cell body rind). Thus, in postembryonic development, the share of the subesophageal ganglion and neuropil in the total volume of the CNS increased, whereas that of the brain and cortex decreased. The mode of the CNS development in E. atrica is similar to that observed in other arthropods, including Argiope aurantia, a spider of different ecology and behaviour.

Bicephality, a seldom occurring developmental deformity in Tegenaria atrica caused by alternating temperatures.

The experiment was aimed at demonstrating the relationship between deformities of the front part of the prosoma accompanied by changes in the brain structure in bicephalous Tegenaria atrica and exposure of their embryos to temperature fluctuations. By exposing spider embryos to alternating temperatures of 14 and 32°C for the first 10 days of embryonic development, we obtained eight two-headed individuals, subsequently divided into three groups according to morphological differences. We described in detail morphological abnormalities of the prosoma identified in members of each group. Histological examination confirmed a close relationship between morphological deformities and the brain structure of teratogenically changed spiders. The fusion of appendages (pedipalps and chalicerae) was accompanied by the fusion of corresponding ganglia. The absence of appendages (pedipalps) was accompanied by the absence of corresponding ganglia. This correlation may have resulted from previously impaired neuromere development which led to changes in the morphological structure of the prosoma. Since no deformities were identified in control animals, it can be concluded that bicephaly was caused by exposing embryos to alternating temperatures.

Teratological deformities of pedipalps in the Tegenaria atrica spider, induced by low and high temperatures applied alternately.

The study was aimed at demonstrating the relationship between temperature (as a factor which disturbs morphogenesis) and deformities in the front part of the prosoma in Tegenaria atrica. By exposing spider embryos to alternating temperatures of 14 and 32°C for the first 10 days of embryonic development, i.e. until the first metameres of the prosoma appeared on the germ band, we obtained individuals with a range of anomalies including those which affected the prosomal morphology. We selected five spiders with deformities of pedipalps or of pedipalps and walking legs for comprehensive analysis. The results indicate a relationship between temperature applied as a teratogenic factor and anomalies in the front part of the prosoma.

Morphometric Changes of the Central Nervous System of Oligomelic Tegenaria atrica Spiders.

Oligomely is an anomaly manifested in the morphology of spiders (except for deformations of the prosoma and exoskeleton), by the absence of one or more appendages, and in their anatomy by the absence of neuromeres. This study was aimed at determining whether there is a correlation between the absence of a neuromere or its half in the subesophageal ganglia and the volume of the prosoma. Morphometric studies involved oligomelic specimens of Tegenaria atrica with the absence of one walking leg and two walking legs. Volumetric analysis concerned with nymph stage II of spiders obtained after exposing the embryos to alternating temperatures of 14 and 320C. The results were compared with those obtained from the histological analysis of the prosoma and central nervous system of control individuals. It was found that there was no relationship between the absence of half or an entire neuromere and the volume of the prosoma of oligomelic specimens. The volume of the central nervous system decreased but the volume change was not proportional to the changes in the prosoma volume. During studies, it was found that the lack of neuromeres resulted in an increase in the volume of remaining neuromeres.

Morphological and anatomical changes related to leg anomalies in Tegenaria atrica.

A range of leg anomalies was detected in embryos of the Tegenaria atrica spiders exposed to alternating temperatures of 14 and 32 °C. Multiple anomalies were observed in 13 individuals. This study is based on five individuals: two individuals affected by oligomely combined, respectively, with heterosymely and polymely, one affected by polymely with heterosymely, one by complicated polymely (accompanied by the reduction in length and malformations of the distal parts of the legs), and one individual with pure polymely. Changes in the central nervous system of these five individuals were described in detail on the basis of histological sections. The changes were mainly related to the number of neuromeres. Individuals affected by polymely had additional ganglia corresponding to the number of additional appendages, whereas the absence of a leg (oligomely) was associated with the absence of a ganglion. Histological analysis showed the fusion of ganglia in the three polymelic specimens, even though additional appendages were not fused.

The central nervous system of heterosymelic individuals of the spider Tegenaria atrica.

This paper presents the results of research on the nervous system in individuals of Tegenaria atrica with heterosymely of walking legs, a pedipalp and the first walking leg. Temperatures alternating between 14 degrees and 32 degrees C every 12 hours were applied as a teratogenic factor in the early stage of embryogenesis. From the thus obtained specimens, histological sections were taken using the paraffin method and then stained. Analysis of the nervous system in individuals with partial heterosymely of walking legs showed no significant changes in the structure of subesophageal nervous mass. In most of the studied individuals with partial and total heterosymely of a pedipalp and walking leg no changes were observed in the relevant parts of the nervous system either, although in two cases a fusion of nerve ganglia was noted, from which the nerves branched off towards the conjoined legs.

An anomaly within the genital system in a female Ascaris suum Goeze.

The authors describe an individual of the female Ascaris suum Goeze with a unique genital system. A female with such an anomaly was found during laboratory classes of Invertebrate Zoology at the Faculty of Biology and Environmental Protection, NCU. The specimen was about 200 mm long, and the total length of the reproductive system was 1970 mm. Further comparative analysis between unchanged and changed individuals revealed differences in the length of individual sections of the studied system, as well as in the diameter of the uterus. The described case is extremely interesting because of the phenomenon of eutely occurring in nematodes. The exact cause and mechanism of abnormalities described in Ascaris suum are not known and difficult to explain experimentally because of the extremely small number of these anomalies. Moreover, the interpretation of the anomaly is difficult because of the specific behavior and complex morphogenesis of this endoparasite.