Standard medical ethnobotany of Kohistan, North Pakistan.

BACKGROUND: This study was exclusively focused on the documentation and cross-cultural evaluation of ethnomedicinal knowledge (EMK) within the diverse linguistic groups of Kohistan situated between the Himalayan and Hindukush Mountain ranges in the north Pakistan. METHODS: Data were gathered during the field survey (May 2022 to July 2023) through group conversations, semi-structured interviews, and on-site observation. Venn diagrams were employed to illustrate the comparative assessment of EMK, and different ethnobotanical indices were utilized to examine the data. RESULTS: A total of 96 wild medicinal plant species (MPs) belonging to 74 genera and 52 botanical families were documented. The most reported MPs belong to the family Polygonaceae (11 species), followed by Asteraceae (9 species) and Lamiaceae (8 species). The ethnomedicinal uses of Leontopodium himalayanum, Pedicularis oederi, Plocama brevifolia, Polypodium sibiricum, Pteridium esculentum, Sambucus wightiana, Solanum cinereum, Teucrium royleanum, Rhodiola integrifolia, Aconitum chasmanthum were reported for the first time in this region. Among the reported taxa herbaceous species were dominated (72%), followed by trees and shrubs (17% and 10%, respectively). Digestive problems (40 taxa and 114 use reports) and skin disorders (19 taxa and 549 use reports) were the most cited disease categories, whereas M. communis, M. longifolia, Ajuga integrifolia, Ziziphus jujuba, and Clematis grata exhibited the highest percentage fidelity levels. Out of 109 documented medicinal uses, a mere 12 were shared across all linguistic groups, and Bateri emerges as a notable outlier with the highest number of medicinal uses. In addition, a significant homogeneity was noted in the reported botanical taxa (61 species) among different linguistic groups. However, since the last decade biocultural heritage of Kohistan is facing multifaceted risks that need urgent attention. CONCLUSION: Our findings could be valuable addition to the existing stock of ethnomedicinal knowledge and may provide ethnopharmacological basis to novel drug discovery for preexisting and emerging diseases prioritizing detailed phytochemical profiling and the evaluation of bioactive potential.

Enhanced extracellular vesicles mediated uttroside B (Utt-B) delivery to Hepatocellular carcinoma cell: Pharmacokinetics based on PBPK modelling.

Our prior investigation has confirmed that the anti-hepatocellular carcinoma activity of the plant saponin, specifically Uttroside B (Utt-B), derived from the leaves of Solanum nigrum Linn. This study concentrated on formulating a novel biocompatible nanocarrier utilizing Extracellular vesicles (EVs) to enhance the delivery of plant saponin into cells. The physicochemical attributes of Extracellular Vesicles/UttrosideB (EVs/Utt-B) were comprehensively characterized through techniques such as Transmission Electron Microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR). Despite the promising therapeutic potential of this uttroside B, mechanistic know-how about its entry into cells is still in its infancy. Our research sheds light on the extracellular vesicle-mediated mechanism facilitating the entry of the saponin into cells, a phenomenon confirmed through the use of by confocal microscopy. We further analysed drug-releasing kinetics and simulated the Pharmacokinetics by PBPK modelling. The simulated pharmacokinetics revealed the bioavailability of Uttroside-B in oral administration against intravenous administration.

Nano-remediation of toxic heavy metal contamination: Hexavalent chromium [Cr(VI)].

The inexorable industrialization and modern agricultural practices to meet the needs of the increasing population have polluted the environment with toxic heavy metals such as Cr(VI), Cu2+, Cd2+, Pb2+, and Zn2+. Among the hazardous heavy metal(loid)s contamination in agricultural soil, water, and air, hexavalent chromium [Cr(VI)] is the most virulent carcinogen. The metallurgic industries, tanneries, paint manufacturing, petroleum refineries are among various such human activities that discharge Cr(VI) into the environment. Various methods have been employed to reduce the concentration of Cr(VI) contamination with nano and bioremediation being the recent advancement to achieve recovery at low cost and higher efficiency. Bioremediation is the process of using biological sources such as plant extracts, microorganisms, and algae to reduce the heavy metals while the nano-remediation uses nanoparticles to adsorb heavy metals. In this review, we discuss the various activities that liberate Cr(VI). We then discuss the various conventional, nano-remediation, and bioremediation methods to keep Cr(VI) concentration in check and further discuss their efficiencies. We also discuss the mechanism of nano-remediation techniques for better insight into the process.

Analysis and simulation of land cover changes and their impacts on land surface temperature in a lower Himalayan region.

Rapid urbanization is changing the existing patterns of Land Use Land Cover (LULC) globally which is consequently increasing the Land Surface Temperature (LST) in many regions. Present study was focused on estimating the current and simulating the future LULC and LST trends in the alpine environment of lower Himalayan region of Pakistan. Past patterns of LULC and LST were identified through the Support Vector Machine (SVM) and multi-spectral Landsat satellite images during 1987-2017 data period. The Cellular automata (CA) model and Artificial Neural Network (ANN) were applied to simulate future (years 2032 and 2047) LULC and LST changes, respectively, using their past patterns. CA model was validated for the simulated and the estimated LULC for the year 2017 with an overall Kappa (K) value of 0.77 using validation modules in QGIS and IDRISI software. ANN method was validated by correlating the observed and simulated LST for the year 2017 with correlation coefficient (R) and Mean Square Error (MSE) values of 0.81 and 0.51, respectively. Results indicated a change in the LULC and LST for instance the built-up area was increased by 4.43% while agricultural area and bare soil were reduced by 2.74% and 4.42%, respectively, from 1987 to 2017. The analysis of LST for different LULC classes indicated that built-up area has highest temperature followed by barren, agriculture and vegetation surfaces. Simulation of future LULC and LST showed that the built-up area will be increased by 2.27% (in 2032) and 4.13% (in 2047) which led 42% (in 2032) and 60% (in 2047) of the study area as compared to 26% area (in 2017) to experience LST greater than 27 °C. A strong correlation between built-up area changes and LST was thus found signifying major challenge to urban planners mitigating the consequent of Urban Heat Island (UHI) phenomenon. It is suggested that future urban planning should focus on urban plantation to counter UHI phenomena in the region of lower Himalayas.

Surface modification of Fe2O3 and MgO nanoparticles with agrowastes for the treatment of chlorosis in Glycine max.

Surface modification of nanoparticles for biological applications is receiving enormous interest among the research community due to the ability to alchemy the toxic nanoparticles into biocompatible compounds. In this study, the agrowastes of Moringa oleifera and Coriandrum sativum were used to surface modify the magnesium oxide nanoparticles and ferric oxide nanoparticles respectively. The agrowaste amended magnesium oxide nano particles (AMNP) and agrowaste amended ferric oxide nanoparticles (AFNP) were characterized using scanning electron microscope, X-ray diffractometer, Fourier transformed-infra red spectroscope to justify the formation and surface modification of nanoparticles with the organic functional groups from the agro wastes. The surface modified nano particles were tested for their biocompatibility and ability to treat the chlorosis in Glycine max. On comparison between the two metal based nanoparticles, AMNP exhibited better chlorosis treating ability than the AFNP. Both the nano particles showed increased potency at minimal amount, 30 µg and the higher concentrations till 125 µg exhibited down run of the potency which was again enhanced from 250 µg of nanoparticle treatment to plants. Further the surface modified nanoparticles were assessed for biocompatibility on human embryonic kidney (HEK-293) cell line which proved that the cell lines are non-toxic to normal human cells. The size of the particles and the concentration is suggested to be responsible for the effective chlorosis treatment and the organic functional groups responsible for the reduction of toxicity of the particles to the plants.