1.
Phytochemistry
; 31(10): 3337-9, 1992 Oct.
Artículo
en Inglés
| MEDLINE
| ID: mdl-1368856
RESUMEN
The large-scale purification of the anti-gibberellin monoclonal antibody, MAC 182, is described. N-Terminal amino acid sequences of the heavy and light chains were determined and compared with those of known antibodies. Fab-fragments were prepared and purified to a state suitable for crystallization.
Asunto(s)
Anticuerpos Monoclonales/aislamiento & purificación , Giberelinas/inmunología , Secuencia de Aminoácidos , Animales , Anticuerpos Monoclonales/química , Anticuerpos Monoclonales/inmunología , Hibridomas , Fragmentos Fab de Inmunoglobulinas/aislamiento & purificación , Cadenas Pesadas de Inmunoglobulina/química , Cadenas Ligeras de Inmunoglobulina/química , Datos de Secuencia Molecular , Ratas
2.
Phytochemistry
; 29(4): 1041-5, 1990.
Artículo
en Inglés
| MEDLINE
| ID: mdl-1366424
RESUMEN
Two new monoclonal antibodies to 3 beta-hydroxy-gibberellins are described. Monoclonal antibodies GA1-1 and GA1-2 were derived from immunizations with the hapten-protein conjugate gibberellin A1-17-KLH. Cross-reactivities with a panel of 43 gibberellins and gibberellin derivatives are compared with those of other anti-gibberellin monoclonal antibodies.