Bi-decadal trend of atmospheric emissions from thermal power plants in Mainland Southeast Asia: Implications on acid deposition and climate change Mitigation.

Steady increase in electricity generation and heavy reliance on coal in Mainland Southeast Asia (M-SEA) create huge pressure on the environment. This study used information collected from individual thermal power plants (TPPs) in M-SEA to calculate emissions of air pollutants and greenhouse gases (GHG) for 2010, 2015 and 2019. The emissions were projected to 2030 following the latest national Power Development Plans. The emission results were analyzed in relation to the power development by country and fuel type, and environmental impacts. The region collective annual TPP emissions in 2019, in Gg/yr, were 27 PM2.5, 77 PM10, 0.7 BC, 4.9 OC, 255 SO2, 451 NOx, 91 CO, 12 NMVOC, 0.4 NH3, 260 CO2, 13 CH4, and 26 N2O. Coal-fired TPPs dominated the emissions of most species while NG-fired contributed the largest amounts of NH3 and CH4. Bi-decadal increase in energy production from TPPs of nearly 3 times is accompanied by 2.7 times increase in emissions. The 2010-2019 period saw average emissions increase by 1.9 times (TPPs' energy production increased 1.6 times), slightly higher than the rate of 1.4 times projected for 2019-2030 (double TPPs' energy production). The current intrusion rate of renewable energy accompanied by phasing-out of old TPPs are still by far insufficient to reverse the emission trend. Aggressive power development in Vietnam with its heavy coal reliance made it the largest emitter in 2019 and the projected for 2030, followed by Thailand. Spatially, higher emissions are seen over locations of large coal-fired TPPs in Vietnam and Thailand. Available rainwater composition monitoring data showed higher deposition amounts of sulfate and nitrate in areas located near or downwind of large TPPs. Significant GHG emissions projected for TPPs in 2030 indicated that TPPs should be the priority for emission reduction to achieve Nationally Determined Contribution targets. Emission database produced by this study can be used in dispersion modeling studies to assess impacts of TPPs on air quality, health, and acid deposition.

Preparation and characterization of cellulose nanocrystals from corncob via ionic liquid [Bmim][HSO4] hydrolysis: effects of major process conditions on dimensions of the product.

In this study, cellulose nanocrystals were prepared via the hydrolysis of corncob (CC) biomass using Brønsted acid ionic liquid 1-butyl-3-methylimidazolium hydrogen sulfate [Bmim][HSO4]. The corncob was subjected to alkaline pretreatment, and was then hydrolysed by [Bmim][HSO4], which acted as both solvent and catalyst. The effects of process conditions, including mass percent of CC (1.0-10.0%), reaction temperature (46-110 °C), and reaction time (1.2-2.8 h) on the size of cellulose nanocrystals (IL-CCCNC) were investigated by response surface methodology-central composite design. The obtained IL-CCCNC was characterized by Fourier transforms infrared spectroscopy, zeta sizer, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and thermogravimetry. The results showed that the dimensions of the nanocellulose products were affected by the mass percent of CC and the reaction temperature, but were not significantly influenced by the reaction time under the studied conditions. The optimal conditions, estimated by the developed model, were a mass percent of 2.49%, reaction temperature of 100 °C, and reaction time of 1.5 h. The process successfully produced IL-CCCNC with a yield of 40.13%, average size of 166 nm, and crystallinity index (CrI) of 62.5%. The morphology, chemical fingerprints, and thermal properties of the obtained IL-CCCNC were comparable to those extracted by alkaline and acid hydrolysis. After the reaction, [Bmim][HSO4] could be recovered with a yield of 88.32%, making it a viable green catalyst for the hydrolysis of CC cellulose. The findings are of direct industrial relevance as optimal processes can be developed to produce nanocellulose crystals with desirable size and physicochemical characteristics.

Effect of blanching pretreatment and microwave-vacuum drying on drying kinetics and physicochemical properties of purple-fleshed sweet potato.

In this study, drying kinetics and quality of purple-fleshed sweet potato (PFSP) subjected to microwave-vacuum drying were investigated. The effects of hot water and steam blanching pretreatment on physicochemical characteristics of the dried products were also considered. The samples were dehydrated in a custom-made microwave-vacuum system at different power levels including 450, 600 and 850 W. Hot air drying at 70 °C was also conducted for comparison. The results showed that drying time of PFSP under microwave-vacuum conditions ranged from 6 to 12 min, significantly reduced as compared to that of hot air drying (600 min). The improvement of drying rate was also evidenced by increased effective moisture diffusivity (2.22 × 10-7-4.05 × 10-7 m2/s) of the samples. Drying kinetics of PFSP was best fitted by Page and logarithmic model with R2 ranging from 0.991 to 0.998, and RMSE from 0.016 to 0.030. PFSP dried under microwave-vacuum condition had lower water absorption index and swelling capacity than hot air drying. Color, antioxidant activity and total phenolic content of dried PFSP were also improved under microwave-vacuum drying. The effects of blanching pretreatment on quality of dried PFSP were more dominant in hot air than microwave-vacuum dried samples.

Assessing the photodynamic efficacy of different photosensitizer-light treatments against foodborne bacteria based on the number of absorbed photons.

Increasing interests in photodynamic treatment (PDT) for food preservation require a holistic method to evaluate and compare different photosensitizer (PS)-light treatments. In this report, the absorbed photons were used as the basis to assess the antimicrobial photodynamic efficacy of two PSs, chlorophyllin sodium magnesium salt (Chl-Mg) and chlorophyllin sodium copper salt (Chl-Cu), under blue and white light against two typical foodborne pathogens, Gram-negative Escherichia coli, and Gram-positive Staphylococcus aureus. The results showed that the phototoxicity of a PS was predominantly decided by the absorbed photons rather than the characteristics of light sources. Photosensitized Chl-Mg exhibited superior antimicrobial activity as compared to that of ChlCu. The applied treatments were found to be more effective against S. aureus than E. coli. Bacterial inactivation kinetics as a function of the number of absorbed photons could be described by Weibull model with R2 from 0.947-0.962, and kinetics constants D in the range of 0.202 × 1017 photons/cm2-2.409 × 1018 photons/cm2. The kinetics models may find promising applications in the design, assessment, and optimization of PDT processes.

Epitope-imprinted polydopamine electrochemical sensor for ovalbumin detection.

A novel, sensitive and selective electrochemical sensor based on epitope-imprinted polydopamine (PDA) was developed for ovalbumin (OVA) detection. Molecularly imprinted polydopamine was synthesized on an AuNP-coated screen-printed carbon electrode (SPCE) via electropolymerization in the presence of OVA IgE-binding epitope as the template. Key process parameters including template concentration, electropolymerization cycle, pH, time required for template removal and rebinding were optimized. Electrochemical detection of OVA was performed by differential pulse voltammetry (DPV) in 5 mM K3Fe(CN)6 and 0.1 M KCl as the supporting electrolyte. Under optimized conditions, the sensor demonstrated excellent sensitivity toward OVA with linear range from 23.25 to 232.50 nM (1 to 10 ppm), limit of detection (LOD) of 10.76 nM (0.46 ppm), and limit of quantification (LOQ) of 35.87 nM (1.54 ppm). The sensor also exhibited good selectivity against other proteins such as human serum albumin (HSA), bovine serum albumin (BSA), and lysozyme (LYZ). OVA in wine samples was detected with RSD of 5.63-10.82%, and recovery percentage of 104.74-105.96%. The developed method can be easily adapted to detect other allergic proteins in the food supply chain.

Epitope-imprinted polymers: applications in protein recognition and separation.

Molecularly imprinted polymers (MIPs) have evolved as promising platforms for specific recognition of proteins. However, molecular imprinting of the whole protein molecule is complicated by its large size, conformational instability, and structural complexity. These inherent limitations can be overcome by using epitope imprinting. Significant breakthroughs in the synthesis and application of epitope-imprinted polymers (EIPs) have been achieved and reported. This review highlights recent advances in epitope imprinting, from the selection of epitope peptide sequences and functional monomers to the methods applied in polymerization and template removal. Technological innovations in detection and extraction of proteins by EIPs are also provided.

Antimicrobial photodynamic efficacy of selected natural photosensitizers against food pathogens: Impacts and interrelationship of process parameters.

Photodynamic treatment (PDT) could be a viable option to decontaminate food or food contact surfaces. Such applications require a rigorous method to assess the efficacy of different photosensitizer-light source systems. It is also essential to determine suitable treatment conditions to achieve desirable microbial inhibition for a given process. In this connection, we evaluated and compared the antimicrobial activity of two natural photosensitizers (aloe emodin, curcumin) under PDT based on the number of absorbed photons. The degree of bacterial inactivation was then correlated to the absorbed photons as well as the process parameters through kinetics study. The results showed that aloe emodin was more effective than curcumin against both S. aureus and E. coli when the number of absorbed photons was matched. Aloe emodin reduced about 2.3 log units of S. aureus and 1.1 log units of E. coli more than curcumin. E. coli was more resistant to PDT than S. aureus. Inactivation kinetics of S. aureus and E. coli as a function of the number of absorbed photons can be described by the Weibull model with D values of 1.296 × 1017 photons/cm2 and 2.446 × 1018 photons/cm2, R2 of 0.969 and 0.968, respectively. The interrelationship between the concentration of photosensitizer, radiant fluence, and degree of bacterial inactivation could be used to determine and optimize treatment conditions of PDT processes.

Programmable electrochemical flow system for high throughput determination of total antioxidant capacity.

The aim of this work was to develop a programmable flow system for rapid assessment of total antioxidant capacity (TAC). Novel features of the prototype include a single pressure-driven source, versatile manipulation of fluid flows, ability to adapt to different TAC assays, and compatibility with microfluidic design. Antioxidant activity was determined by electrochemical measurement of residual 2,2-diphenyl-2-picrylhydrayl hydrate (DPPH•) free radicals in the solution. The overall performance of the device was validated by the spectrophotometric method. The results indicated that dosing of reagents and samples could be controlled by pressure (R2 = 0.992) and time (R2 = 0.999) with high accuracy, and the mixing uniformity of the device was equivalent to that of the batch mixing (R2 = 0.994). TAC assays of a standard antioxidant, ascorbic acid, as well as selected samples such as orange and pomegranate juices, white wine, and green tea by the device were comparable to traditional measurement techniques. Due to the short incubation time, the approach may be more suitable for fast, rather than slow reacting antioxidant compounds. The developed system could be used for rapid TAC screening of food and biological samples.

Development of a portable electrochemical loop mediated isothermal amplification (LAMP) device for detection of hepatitis B virus.

The objective of this study was to develop a simple, inexpensive prototype device for rapid detection of hepatitis B virus (HBV). The device was able to simultaneously amplify, detect and quantify the target HBV DNA. The system was fabricated from a custom-made electrochemical set-up of which the temperature was thermostatically controlled by a water bath. Real-time monitoring of HBV DNA was accomplished by measuring the response of redox indicator in the reaction mixture. Concentration of HBV DNA in the samples was determined from the peak high ratio (PHR) and threshold time relationship. The signal was processed by sigmoidal model fitting to enhance the accuracy of the results. Key parameters including concentrations of redox indicator and reaction temperatures were optimized. Sensitivity and specificity of the method toward HBV DNA were evaluated. The prototype was capable of real-time amplification and detection of HBV DNA with concentration as low as 6.18 fg µl-1. The test showed high specificity against HBV DNA. The system was also able to detect HBV positive serum directly with simple thermal pretreatment instead of tedious DNA extraction. The electrochemical set-up was compatible with microfluidic platforms and can be readily adapted for efficient and high throughput point-of-care (POC) diagnosis of HBV.