RESUMEN
The choline-glycine betaine pathway plays an important role in bacterial survival in hyperosmotic environments. Osmotic activation of the choline transporter BetT promotes the uptake of external choline for synthesizing the osmoprotective glycine betaine. Here, we report the cryo-electron microscopy structures of Pseudomonas syringae BetT in the apo and choline-bound states. Our structure shows that BetT forms a domain-swapped trimer with the C-terminal domain (CTD) of one protomer interacting with the transmembrane domain (TMD) of a neighboring protomer. The substrate choline is bound within a tryptophan prism at the central part of TMD. Together with functional characterization, our results suggest that in Pseudomonas species, including the plant pathogen P. syringae and the human pathogen Pseudomonas aeruginosa, BetT is locked at a low-activity state through CTD-mediated autoinhibition in the absence of osmotic stress, and its hyperosmotic activation involves the release of this autoinhibition.
Asunto(s)
Proteínas Bacterianas , Colina , Microscopía por Crioelectrón , Proteínas de Transporte de Membrana , Proteínas de Transporte de Membrana/metabolismo , Proteínas de Transporte de Membrana/química , Colina/metabolismo , Colina/química , Proteínas Bacterianas/metabolismo , Proteínas Bacterianas/química , Pseudomonas syringae/metabolismo , Modelos Moleculares , Osmorregulación , Presión Osmótica , Betaína/metabolismo , Conformación Proteica , Unión Proteica , Relación Estructura-Actividad , Dominios ProteicosRESUMEN
BACKGROUND: Fleas, considered to be the main transmission vectors of Bartonella, are highly prevalent and show great diversity. To date, no investigations have focused on Bartonella vectors in Southeast China. The aim of this study was to investigate the epidemiological and molecular characteristics of Bartonella in fleas in Southeast China. METHODS: From 2016 to 2022, flea samples (n = 1119) were collected from 863 rodent individuals in seven inland and coastal cities in Southeast China. Flea species, region, gender, host species and habitat were recorded. The DNA samples from each individual flea were screened by real-time PCR for the Bartonella ssrA gene. All positive samples were confirmed by PCR based on the presence of the gltA gene and sequenced. The factors associated with Bartonella infection were analyzed by the Chi-square test and Fisher's exact test. ANOVA and the t-test were used to compare Bartonella DNA load. RESULTS: Bartonella DNA was detected in 26.2% (293/1119) of the flea samples, including in 27.1% (284/1047) of Xenopsylla cheopis samples, 13.2% (5/38) of Monopsyllus anisus samples, 8.3% (2/24) of Leptopsylla segnis samples and 20.0% (2/10) of other fleas (Nosopsyllus nicanus, Ctenocephalides felis, Stivalius klossi bispiniformis and Neopsylla dispar fukienensis). There was a significant difference in the prevalence of Bartonella among flea species, sex, hosts, regions and habitats. Five species of Bartonella fleas were identified based on sequencing and phylogenetic analyses targeting the gltA gene: B. tribocorum, B. queenslandensis, B. elizabethae, B. rochalimae and B. coopersplainsensis. CONCLUSIONS: There is a high prevalence and diversity of Bartonella infection in the seven species of fleas collected in Southeast China. The detection of zoonotic Bartonella species in this study, including B. tribocorum, B. elizabethae and B. rochalimae, raises public health concerns.
Asunto(s)
Infecciones por Bartonella , Bartonella , Infestaciones por Pulgas , Variación Genética , Insectos Vectores , Roedores , Siphonaptera , Animales , Bartonella/genética , Bartonella/aislamiento & purificación , Bartonella/clasificación , China/epidemiología , Siphonaptera/microbiología , Infecciones por Bartonella/epidemiología , Infecciones por Bartonella/veterinaria , Infecciones por Bartonella/microbiología , Infecciones por Bartonella/transmisión , Roedores/microbiología , Femenino , Infestaciones por Pulgas/epidemiología , Infestaciones por Pulgas/veterinaria , Infestaciones por Pulgas/parasitología , Insectos Vectores/microbiología , Masculino , Filogenia , ADN Bacteriano/genética , Enfermedades de los Roedores/epidemiología , Enfermedades de los Roedores/microbiología , Enfermedades de los Roedores/parasitología , PrevalenciaRESUMEN
Papaya (Carica papaya L.) is a climacteric fruit susceptible to postharvest losses attributable to ethylene-induced ripening and softening. In this study, we examined the effect of 1-methylcyclopropene (1-MCP) treatment (1 µL L-1 for 20 h) on the textural properties of "SunUp" papaya fruit and investigated the regulatory mechanisms of molecular profiles. Compared with control, postharvest 1-MCP treatment significantly inhibited fruit softening, which is associated with higher hemicellulose content and lower xylanase activity of papaya fruit. Moreover, RNA-seq and qRT-PCR analyses indicated that CpbHLH3 and CpXYN1 were differentially expressed during storage. Yeast one-hybrid, electrophoretic mobility shift assays, and dual-luciferase reporter assays disclosed that CpbHLH3 activated the transcription of CpXYN1 by binding directly to its promoter. Transient overexpression of CpbHLH3 alleviates the inhibitory effect of 1-MCP on softening by increasing xylanase activity and upregulating the gene expression. Our observations provide new insights into the transcriptional regulatory mechanisms that govern softening of postharvest papaya fruit.
Asunto(s)
Carica , Carica/genética , Carica/metabolismo , Ciclopropanos , Frutas/metabolismo , Regulación de la Expresión Génica de las Plantas , Proteínas de Plantas/metabolismo , Factores de Transcripción/genética , Verduras/metabolismoRESUMEN
Scorpiothyrsus erythrotrichus belongs to Melastomataceae. Here, we present its complete plastome. To our knowledge, this is the first reported complete chloroplast genome of S. erythrotrichus. The complete plastome of S. erythrotrichus is 160,731 bp in length with a typical quadripartite structure, consisting of four regions: large single-copy (LSC) region (85,483 bp), small single-copy (SSC) region (17,007 bp), and two inverted repeat regions (IRs, 26,780 bp). It contains 128 genes (79 coding genes, four rRNAs, and 30 tRNAs). The overall GC content is 36.9% and in the LSC, SSC, and IR regions are 34.70%, 30.40%, and 42.50%, respectively. Our study contributes to the molecular phylogenetic studies of Scorpiothyrsus and Melastomataceae.