RESUMEN
Long-term preservation of gametes has been identified as a tool to improve broodstock management and increase the number of juveniles produced by artificial fertilization. Paralichthys orbignyanus is an important commercial and recreational species distributed in marine and estuarine waters from Rio de Janeiro (Brazil) to the San Matías Gulf (Argentina). This work focused on studying the seminal quality of tank-reared P. orbignyanus, demonstrating that males are fluent year-round, with the highest yields at the early reproductive season. Fresh sperm exhibited good forward swimming, and samples could be refrigerated up to 48 h while retaining their motility after activation. The optimal conditions for P. orbignyanus sperm motility activation were established as 950 mOsmol/Kg and pH values between 7 and 7.9. Additionally, a well-defined protocol for semen vitrification was developed to assess the cryotolerance of this species' sperm. We successfully produced high-quality sperm samples, using two vitrification formulations containing trehalose and both z-1000 and x-1000 polymers, that can be used in a near-future in vitro embryo production program.
RESUMEN
Survival of tumor cells is favored by mitochondrial changes that make death induction more difficult in a variety of stress conditions, such as exposure to chemotherapeutics. These changes are not fully characterized in tumor mitochondria, and include unbalance of the redox equilibrium, inhibition of permeability transition pore (PTP) opening through kinase signaling pathways and modulation of members of the Bcl-2 protein family. Here we show that a novel chemotherapeutic, the Gold(III)-dithiocarbamato complex AUL12, induces oxidative stress and tumor cell death both favoring PTP opening and activating the pro-apoptotic protein Bax of the Bcl-2 family. AUL12 inhibits the respiratory complex I and causes a rapid burst of mitochondrial superoxide levels, leading to activation of the mitochondrial fraction of GSK-3α/ß and to the ensuing phosphorylation of the mitochondrial chaperone cyclophilin D, which in turn facilitates PTP opening. In addition, following AUL12 treatment, Bax interacts with active GSK-3α/ß and translocates onto mitochondria, where it contributes to PTP induction and tumor cell death. These findings provide evidence that targeting the redox equilibrium maintained by mitochondria in tumor cells allows to hit crucial mechanisms that shield neoplasms from the toxicity of many anti-tumor strategies, and identify AUL12 as a promising chemotherapeutic compound.
Asunto(s)
Antineoplásicos/farmacología , Glucógeno Sintasa Quinasa 3/metabolismo , Mitocondrias/enzimología , Neoplasias/metabolismo , Estrés Oxidativo , Proteína X Asociada a bcl-2/metabolismo , Animales , Apoptosis/efectos de los fármacos , Muerte Celular , Permeabilidad de la Membrana Celular/efectos de los fármacos , Glucógeno Sintasa Quinasa 3/genética , Glucógeno Sintasa Quinasa 3 beta , Humanos , Ratones , Ratones Endogámicos BALB C , Mitocondrias/efectos de los fármacos , Mitocondrias/metabolismo , Neoplasias/enzimología , Neoplasias/genética , Estrés Oxidativo/efectos de los fármacos , Unión Proteica/efectos de los fármacos , Proteína X Asociada a bcl-2/genéticaRESUMEN
The present study examined blood and urinary concentrations of Cr and Co in 30 patients with metal-on-metal hip prostheses without signs of wear and 6 patients with prosthetic bearing and clear signs of wear and metallosis. The determination in biological fluids showed in patients with not signs of wear the geometric mean concentration of metals only modestly increased (CoS 0.5 microg/l, CoU 5.7 microg/l, CrS 0.8 microg/l, CrU 3.4 microg/l) compared to the reference values, while the wear caused a significant increase in the concentration of both Co (CoS 94.6 microg/l, CoU 334.5 microg/l) and Cr (CrS 57.7 microg/l, CrU 89.4 microg/I). As the results, the not functioning implants are a risks to the patients and are associated with high levels of metals in biological fluids. Currently, the patients with metallosis had not signs and symptoms associated with metal toxicity, but high concentrations could to cause kidney, peripheral nervous system, heart, and thyroid damage.
Asunto(s)
Cromo/sangre , Cromo/orina , Cobalto/sangre , Cobalto/orina , Prótesis de Cadera/efectos adversos , Prótesis Articulares de Metal sobre Metal/efectos adversos , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Certain esterase inhibitors, such as carbamates, phosphinates and sulfonyl halides, do not cause neuropathy as some organophosphates, but they may exacerbate chemical or traumatic insults to axons. This phenomenon is called promotion of axonopathies. Given the biochemical and toxicological characteristics of these compounds, the hypothesis was made that the target of promotion is a phenyl valerate (PV) esterase similar to neuropathy target esterase (NTE), the target of organophosphate induced delayed polyneuropathy. However, attempts to identify a PV esterase in hen peripheral nerve have been, so far, unsuccessful. We tested several esters, other than PV, as substrates of esterases from crude homogenate of the hen peripheral nerve. The ideal substrate should be poorly hydrolysed by NTE but extensively by enzyme(s) that are insensitive to non-promoters, such as mipafox, and sensitive to promoters, such as phenyl methane sulfonyl fluoride (PMSF). When phenyl benzoate (PB) was used as substrate, about 65% of total activity was resistant to the non-promoter mipafox (up to 0.5 mM, 20 min, pH 8.0), that inhibits NTE and other esterases. More than 90% of this resistant activity was sensitive to the classical promoter PMSF (1 mM, 20 min, pH 8.0) with an IC(50) of about 0.08 mM (20 min, pH 8.0). On the contrary, the non-promoter p-toluene sulfonyl fluoride caused only about 10% inhibition at 0.5 mM. Several esterase inhibitors including, paraoxon, phenyl benzyl carbamate, di-n-butyl dichlorovinyl phosphate and di-isopropyl fluorophosphate, were tested both in vitro and in vivo for inhibition of this PB activity. Mipafox-resistant PMSF-sensitive PB esterase activity(ies) was inhibited by promoters but not by non promoters and neuropathic compounds.
Asunto(s)
Benzoatos/toxicidad , Hidrolasas de Éster Carboxílico/antagonistas & inhibidores , Inhibidores Enzimáticos/toxicidad , Nervio Ciático/efectos de los fármacos , Animales , Pollos , Isoflurofato/análogos & derivados , Isoflurofato/toxicidad , Fluoruro de Fenilmetilsulfonilo/toxicidad , Nervio Ciático/enzimología , Especificidad por SustratoRESUMEN
This review summarizes recent work on the regulation of the permeability transition pore, a cyclosporin A-sensitive mitochondrial channel that may play a role in intracellular calcium homeostasis and in a variety of forms of cell death. The basic bioenergetics aspects of pore modulation are discussed, with some emphasis on the links between oxidative stress and pore dysregulation as a potential cause of mitochondrial dysfunction that may be relevant to cell injury.
Asunto(s)
Permeabilidad de la Membrana Celular , Mitocondrias/ultraestructura , Animales , Calcio/metabolismo , Muerte Celular , Humanos , Mitocondrias/metabolismo , Translocasas Mitocondriales de ADP y ATP/metabolismo , Estrés OxidativoRESUMEN
Mitochondria possess an inner membrane channel, the permeability transition pore, which is inhibited by cyclosporin A (CsA) and by matrix protons. As suggested recently by our laboratory, pore closure by these inhibitors may be due to dissociation of mitochondrial cyclophilin (CyP-M), a matrix peptidyl-prolyl-cis-trans isomerase, from its putative binding site on the pore. Unbinding of CyP-M would follow a CsA-dependent or proton-dependent change in conformation of the CyP-M molecule. It is interesting that upon binding of CsA the enzymatic activity of CyP-M is inhibited, but it is not clear whether this event plays a role in pore inhibition. Here we report experiments designed to further test the role of CyP-M in pore function. Our results indicate that CyP-M-dependent and independent mechanisms of pore activation may exist, and that the peptidylprolyl-cis-trans-isomerase activity of CyP-M is not necessarily involved in pore modulation by CyP-M.
Asunto(s)
Mitocondrias Hepáticas/metabolismo , Isomerasa de Peptidilprolil/metabolismo , Animales , Ciclosporina/farmacología , Membranas Intracelulares/metabolismo , Canales Iónicos/efectos de los fármacos , Canales Iónicos/metabolismo , Canales Iónicos/ultraestructura , Mitocondrias Hepáticas/ultraestructura , Permeabilidad/efectos de los fármacos , RatasRESUMEN
Mammalian mitochondria possess an inner membrane channel, the permeability transition pore (MTP), which can be inhibited by nanomolar concentrations of cyclosporin (CS) A. The molecular basis for MTP inhibition by CSA remains unclear. Mitochondria also possess a matrix cyclophilin (CyP) with a unique N-terminal sequence (CyP-M). To test the hypothesis that it interacts with the MTP, we have studied the interactions of CyP-M with rat liver mitochondria by Western blotting with a specific antibody against its unique N terminus. Although sonication in isotonic sucrose at pH 7.4 refraction sediments with submitochondrial particles at 150,000 x g. We show that the interactions of this CyP-M pool with submitochondrial particles are disrupted (i) by the addition of CSA, which inhibits the pore, but not of CSH, which does not, and (ii) by acidic pH condition, which also leads to selective inhibition of the MTP; furthermore, we show that the effect of acidic pH on CyP-M fully prevents the inhibitory effect of H+ on the MTP (Nicolli, A., Petronilli, V., and Bernardi, P. (1993) Biochemistry 32, 4461-4465). These data suggest that CyP-M inhibition by CSA and protons may be due to unbinding of CyP-M from its putative binding site on the MTP. A role for CyP-M in MTP regulation is also supported by a study with a series of CSA derivatives with graded affinity for CyP. We show that with each derivative the isomerase activity of CyP-M purified to homogeneity is similar to that displayed at inhibition of MTP opening, CyP-M (but not CyP-A) and decreased efficiency at MTP inhibition is obtained by substitution in position 8 while a 4-substituted, nonimmunosuppressive derivative is a as effective as the native CSA molecule, indicating that calcineurin is not involved in MTP inhibition by CSA.
Asunto(s)
Isomerasas de Aminoácido/metabolismo , Proteínas Portadoras/metabolismo , Ciclosporina/metabolismo , Membranas Intracelulares/química , Mitocondrias Hepáticas/química , Isomerasas de Aminoácido/química , Secuencia de Aminoácidos , Animales , Proteínas Portadoras/química , Membranas Intracelulares/metabolismo , Mitocondrias Hepáticas/metabolismo , Datos de Secuencia Molecular , Péptidos/química , Péptidos/metabolismo , Isomerasa de Peptidilprolil , Permeabilidad , Ratas , Partículas Submitocóndricas/química , Partículas Submitocóndricas/metabolismoAsunto(s)
Ciclosporina/farmacología , Canales Iónicos/fisiología , Mitocondrias/fisiología , Daño por Reperfusión/fisiopatología , Nucleótidos de Adenina/metabolismo , Adenosina Trifosfato/metabolismo , Animales , Cationes Bivalentes/farmacología , Humanos , Membranas Intracelulares/fisiología , Activación del Canal Iónico , Canales Iónicos/efectos de los fármacos , Potenciales de la Membrana , Mitocondrias/efectos de los fármacos , Modelos Biológicos , ATPasas de Translocación de Protón/metabolismoRESUMEN
Mitochondria from a variety of sources possess a regulated inner membrane channel, the permeability transition pore (MTP), which is responsible for the 'permeability transition', a sudden permeability increase to solutes with molecular masses < or = 1500 Da, most easily observed after Ca2+ accumulation. The MTP is a voltage-dependent channel blocked by cyclosporin A with Ki in the nanomolar range. The MTP open probability is regulated by both the membrane potential and matrix pH. The probability of pore opening increases as the membrane is depolarized, while it decreases as matrix pH is decreased below 7.3 through reversible protonation of histidine residues. Many physiological and pathological effectors, including Ca2+ and ADP, modulate MTP operation directly through changes of the gating potential rather than indirectly through changes of the membrane potential (Petronilli, V., Cola, C., Massari, S., Colonna, R. and Bernardi, P. (1993) J. Biol. Chem. 268, 21939-21945). Here we present recent work from our laboratory indicating that (i) the voltage sensor comprises at least two vicinal thiols whose oxidation-reduction state affects the MTP gating potential; as the couple becomes more oxidized the gating potential increases; conversely, as it becomes more reduced the gating potential decreases; (ii) that MTP opening is fully reversible, as mitochondria maintain volume homeostasis through several cycles of pore opening/closure; and (iii) that the mechanism of MTP inhibition by cyclosporin A presumably involves a mitochondrial cyclophilin but does not utilize a calcineurin-dependent pathway.
Asunto(s)
Ciclosporina/farmacología , Membranas Intracelulares/efectos de los fármacos , Mitocondrias Hepáticas/efectos de los fármacos , Isomerasas de Aminoácido/fisiología , Animales , Arsenitos/farmacología , Calcio , Proteínas Portadoras/fisiología , Ácido Egtácico , Canales Iónicos/efectos de los fármacos , Potenciales de la Membrana/efectos de los fármacos , Mitocondrias Hepáticas/metabolismo , Isomerasa de Peptidilprolil , Permeabilidad/efectos de los fármacos , Ratas , Transducción de SeñalRESUMEN
Energized mitochondria in sucrose medium take up a Ca2+ pulse but do not show opening of the permeability transition pore (MTP) upon membrane depolarization by uncoupler. This is due to locking of the pore in the closed conformation by matrix acidification and fast Ca2+ efflux following membrane depolarization (Petronilli, V., Cola, C., & Bernardi P. (1993) J. Biol. Chem. 268, 1011-1016). Here we show that addition of diethyl pyrocarbonate (DPC) prior to membrane depolarization restores the ability of uncoupler to induce MTP opening. Since DPC does not modify the rate and extent of matrix acidification and the rate and extent of Ca2+ release following addition of uncoupler, its effects on pore opening appear to be due to modification of histidyl residues regulating the pore open-closed probability. This hypothesis was confirmed in studies with deenergized mitochondria incubated in potassium thiocyanate medium. While at acidic pH values pore opening is otherwise prevented, DPC allows Ca2(+)-dependent pore opening at pH 6.5 in a process that maintains full sensitivity to cyclosporin A. Pore induction by DPC can be completely prevented and partially reversed by hydroxylamine, indicating that the effect of DPC can be specifically traced to carbethoxylation of histidyl residue(s) rather than to reaction with tyrosyl or sulfhydryl groups, while the possible involvement of lysyl residues cannot be excluded. Since DPC increases the pore open probability even at matrix pH values between 7.0 and 7.7, we propose that reversible protonation of one or more histidyl residues on the matrix side of the MTP plays a role in the physiological modulation of pore opening.
Asunto(s)
Calcio/metabolismo , Ciclosporina/farmacología , Dietil Pirocarbonato/farmacología , Histidina , Canales Iónicos/fisiología , Mitocondrias Hepáticas/fisiología , Animales , Carbonil Cianuro p-Trifluorometoxifenil Hidrazona/farmacología , Fluoresceínas , Colorantes Fluorescentes , Concentración de Iones de Hidrógeno , Hidroxilamina , Hidroxilaminas/farmacología , Activación del Canal Iónico/efectos de los fármacos , Canales Iónicos/efectos de los fármacos , Cinética , Mitocondrias Hepáticas/efectos de los fármacos , RatasRESUMEN
Addition of A23187 plus EDTA to rat liver mitochondria induces a common uniport pathway for monovalent cations. In this study, we have carried out a detailed characterization of the flow/force relationship for K+ transport along this pathway under steady state conditions. In the presence of EDTA, the K+ conductance is a linear function of external K+ in the range 0-20 mM K+, with a slope of 0.15 nmol of K+ x mg of protein-1 x min-1 x mV-1. The K+ conductance is inhibited by Mg2+ in the range 10(-9)-10(-6) M, while K+ flux is stimulated by the sulfhydryl group reagent mersalyl. Uniport activity can be detected in native mitochondria. These findings are compatible with the notion that electrophoretic K+ flux across the inner membrane takes place via a regulated K+ uniport with the potential of transporting K+ at rates in excess of 600 nmol x mg of protein-1 x min-1.