Performance optimization and nitrogen removal mechanism of up-flow partial denitrification/anammox process.

This study aimed to remediate the problems of sludge floating and uneven mass transfer in up-flow partial denitrification/anammox (PDA) reactors and dissect the nitrogen removal mechanism. Two up-flow PDA reactors were operated, whereby in R1 combined biological carriers were added, while in R2 mechanical stirring was applied, the reactors were inoculated with PD sludge and anammox sludge. Results showed the TN removal rates at the end of the operation were 89% (R1) and 92% (R2). The addition of both strategies suppressed the occurrence of sludge upwelling and deterioration of settling performance, even when the granule diameter of the granular zone in R1 and R2 reached 1.921 and 2.006 mm, respectively. 16SrRNA sequencing revealed R1 had a higher abundance of anammox bacteria (AAOB, 14.53%-R1, 9.06%-R2, respectively), and R2 had a higher quantity of denitrifying bacteria (61.92%-R1, 67.11%-R2, respectively). And the nitrogen removal was contributed by anammox and denitrification in combination, with contributions of 82.17%, 17.83% (R1), and 85.07%, 14.93% (R2), respectively. In summary, both strategies prevented sludge flotation and uneven nitrogen mass transfer. However, mechanical agitation had a more substantial positive effect on the performance of PDA than the addition of biocarriers because it achieved a more adequate mass transfer.

Culturing partial-denitrification (PD) granules in continuous flow reactor with waste sludge as inoculum: performance, granular sludge characteristics and microbial community.

Partial denitrification granular sludge (PDGS) can provide long-term stable nitrite for anaerobic ammonia oxidation (anammox). The cultivation of ordinary activated sludge from wastewater treatment plants into PDGS can further promote the application of PD in practical engineering. In this study, the feasibility of fast start-up of PDGS was explored by inoculating waste sludge in up-flow anaerobic sludge blanket (UASB) reactor with synergistic control of nitrogen load rate (NLR, 0.05-0.65 kg N/m3/d) and electron donor starvation (EDS) (240-168 mg L-1), and system performance, particle characteristics and microbial structure were studied. The results showed that PD-UASB started successfully within 48 days, the average nitrite accumulation rate (NTR) and nitrate removal ratio (NRR) reached 79.6% and 82.5% after successful initiation, accompanied by high abundance of PD bacteria (Thauera, Pseudomonas, unclassflied commamonadaceae and Limnobacter) (25.3%). The increase of PD activity, and the difference between nitrate reductase (NAR) and nitrite reductase (NIR) contributed to nitrite production. Besides, the sludge shifted from flocculated (≤0.5 mm, 95.37%) to granulated state (0.5-2 mm, 64.74%), which could be due to the increase of extracellular polymers (EPS) (especially T-EPS) and metabolism of specific microorganisms (Bacteroidota and Chloroflexi, 19.92%). Good sludge granulation promoted the settleability of PD (the SVI5 was 47.248 mL/ g. ss after successful start-up). In summary, good PD sludge granulation process could be achieved in a short time by synergistically controlling NLR and EDS.