RESUMEN
Amyloid beta (Abeta) is a main component of senile plaques in Alzheimer's disease and induces neuronal cell death. Reactive oxygen species (ROS), nitric oxide and endoplasmic reticulum (ER) stress have been implicated in Abeta-induced neurotoxicity. We have reported that apoptosis signal-regulating kinase 1 (ASK1) is required for ROS- and ER stress-induced JNK activation and apoptosis. Here we show the involvement of ASK1 in Abeta-induced neuronal cell death. Abeta activated ASK1 mainly through production of ROS but not through ER stress in cultured neuronal cells. Importantly, ASK1-/- neurons were defective in Abeta-induced JNK activation and cell death. These results indicate that ROS-mediated ASK1 activation is a key mechanism for Abeta-induced neurotoxicity, which plays a central role in Alzheimer's disease.
Asunto(s)
Péptidos beta-Amiloides/farmacología , MAP Quinasa Quinasa Quinasa 5/metabolismo , Neuronas/efectos de los fármacos , Especies Reactivas de Oxígeno/metabolismo , Enfermedad de Alzheimer/etiología , Animales , Muerte Celular/efectos de los fármacos , Retículo Endoplásmico/enzimología , Retículo Endoplásmico/metabolismo , Activación Enzimática/efectos de los fármacos , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , MAP Quinasa Quinasa Quinasa 5/genética , Proteínas de la Membrana/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Neuronas/citología , Neuronas/metabolismo , Óxidos de Nitrógeno/metabolismo , Células PC12 , Fragmentos de Péptidos/farmacología , Proteínas Serina-Treonina Quinasas/metabolismo , Ratas , eIF-2 Quinasa/metabolismoRESUMEN
Apoptosis signal-regulating kinase 1 (ASK1) is a MAP kinase kinase kinase (MAPKKK) that activates the JNK and p38 MAP kinase cascades and is activated in response to oxidative stress such as hydrogen peroxide (H(2)O(2)). A yeast two-hybrid screening identified a serine/threonine protein phosphatase 5 (PP5) as a binding partner of ASK1. PP5 directly dephosphorylated an essential phospho-threonine residue within the kinase domain of ASK1 and thereby inactivated ASK1 activity in vitro and in vivo. The interaction between PP5 and ASK1 was induced by H(2)O(2) treatment and was followed by the decrease in ASK1 activity. PP5 inhibited not only H(2)O(2)-induced sustained activation of ASK1 but also ASK1-dependent apoptosis. Thus, PP5 appears to act as a physiological inhibitor of ASK1-JNK/p38 pathways by negative feedback.
Asunto(s)
Retroalimentación Fisiológica/fisiología , Quinasas Quinasa Quinasa PAM/metabolismo , Proteínas Nucleares/metabolismo , Estrés Oxidativo/fisiología , Fosfoproteínas Fosfatasas/metabolismo , Animales , Apoptosis/efectos de los fármacos , Células COS , Línea Celular , Células HeLa/enzimología , Humanos , Peróxido de Hidrógeno/farmacología , Riñón/citología , Riñón/metabolismo , MAP Quinasa Quinasa Quinasa 5 , Quinasas Quinasa Quinasa PAM/antagonistas & inhibidores , Quinasas Quinasa Quinasa PAM/genética , Proteínas Nucleares/genética , Proteínas Nucleares/farmacología , Oxidantes/farmacología , Fosfoproteínas Fosfatasas/genética , Fosfoproteínas Fosfatasas/farmacología , Unión Proteica/efectos de los fármacos , Unión Proteica/fisiología , Transducción de Señal/fisiología , Transfección , Factor de Necrosis Tumoral alfa/farmacología , Técnicas del Sistema de Dos HíbridosRESUMEN
Arsenic trioxide (As2O3) induces clinical remission of patients with acute promyelocytic leukemia. As a novel anticancer agent for treatment of solid cancers, As2O3 is promising, but no in vivo experimental investigations of its efficacy on solid cancers have been done at clinically obtained concentrations. In addition, the cell death mechanism of As2O3 has yet to be clarified, especially in solid cancers. In this study, human androgen-independent prostate cancer cell lines, PC-3, DU-145, and TSU-PR1 were examined as cellular models for As2O3 treatment, and As2O3-induced cell death and inhibition of cell growth and colony formation were evaluated. The involvement of p38, c-Jun NH2-terminal kinase (JNK), caspase-3, and reactive oxygen species (ROS) were investigated in As2O3-induced cell death. Finally, As2O3 was administered to severe combined immunodeficient mice inoculated orthotopically with PC-3 cells to estimate in vivo efficacy. In all three of the cell lines, at high concentrations, As2O3 induced apoptosis and, at low concentrations, growth inhibition. As2O3 activated p38, JNK, and caspase-3 dose dependently. Treatment with the p38 inhibitor and over-expression of dominant-negative JNK did not guard against As2O3-induced cell death. In contrast with partial protection by the caspase-3 inhibitor, the antioxidant N-acetyl-L-cysteine gave marked protection from As2O3-induced apoptosis and eliminated the activation of p38, JNK, and caspase-3, and the generation of ROS. The orthotopic murine metastasis model showed in vivo tumor growth inhibition in orthotopic and metastatic lesions with no signs of toxicity. This study establishes that As2O3 provides a novel, safe approach for treatment of androgen-independent prostate cancer. Generation of ROS as a therapeutic target for the potentiation of As2O3-induced apoptosis also was shown.
Asunto(s)
Antineoplásicos/farmacología , Arsenicales/farmacología , División Celular/efectos de los fármacos , Proteínas Quinasas JNK Activadas por Mitógenos , Metástasis de la Neoplasia/prevención & control , Óxidos/farmacología , Neoplasias de la Próstata/tratamiento farmacológico , Andrógenos/fisiología , Animales , Apoptosis/efectos de los fármacos , Trióxido de Arsénico , Caspasas/metabolismo , Muerte Celular/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Activación Enzimática/efectos de los fármacos , Humanos , MAP Quinasa Quinasa 4 , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones SCID , Quinasas de Proteína Quinasa Activadas por Mitógenos/metabolismo , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Metástasis de la Neoplasia/patología , Trasplante de Neoplasias , Células Madre Neoplásicas/efectos de los fármacos , Neoplasias de la Próstata/patología , Especies Reactivas de Oxígeno/metabolismo , Trasplante Heterólogo , Células Tumorales Cultivadas , Ensayo de Tumor de Célula Madre , Ensayos Antitumor por Modelo de Xenoinjerto , Proteínas Quinasas p38 Activadas por MitógenosRESUMEN
Apoptosis signal-regulating kinase (ASK) 1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H(2)O(2), and activates c-Jun NH(2)-terminal kinase (JNK) and p38. However, the roles of JNK and p38 signaling pathways during apoptosis have been controversial. Here we show that by deleting ASK1 in mice, TNF- and H(2)O(2)-induced sustained activations of JNK and p38 are lost in ASK1(-/-) embryonic fibroblasts, and that ASK1(-/-) cells are resistant to TNF- and H(2)O(2)-induced apoptosis. TNF- but not Fas-induced apoptosis requires ROS-dependent activation of ASK1-JNK/p38 pathways. Thus, ASK1 is selectively required for TNF- and oxidative stress-induced sustained activations of JNK/p38 and apoptosis.
Asunto(s)
Apoptosis/fisiología , Quinasas Quinasa Quinasa PAM/metabolismo , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Animales , Apoptosis/efectos de los fármacos , Células Cultivadas , Activación Enzimática , Peróxido de Hidrógeno/farmacología , Proteínas Quinasas JNK Activadas por Mitógenos , MAP Quinasa Quinasa Quinasa 5 , Quinasas Quinasa Quinasa PAM/genética , Ratones , Ratones Noqueados , Transducción de Señal , Factor de Necrosis Tumoral alfa/farmacología , Receptor fas/farmacología , Proteínas Quinasas p38 Activadas por MitógenosRESUMEN
Apoptosis signal-regulating kinase 1 (ASK1) is a member of the MAPKKK family in the JNK and p38 mitogen-activated protein kinase cascades and critically involved in stress- and cytokine-induced apoptosis. The transcription factor nuclear factor-kappaB (NF-kappaB) is a pivotal regulator of immune and inflammatory responses and exerts anti-apoptotic roles in various cells. Here we show that ASK1 directly interacts with transforming growth factor-beta-activated kinase 1 (TAK1), another MAPKKK that has been identified as a signaling intermediate in the interleukin 1 (IL-1)-induced NF-kappaB pathway as well as the transforming growth factor-beta superfamily-induced JNK/p38 pathway. Overexpression of ASK1 inhibits IL-1-, TRAF6-, or TAK1-induced, but not NF-kappaB-inducing kinase-induced, NF-kappaB activation. ASK1 dissociates TAK1 but not NF-kappaB-inducing kinase from TRAF6. Moreover, IL-1-induced complex formation of endogenous TAK1 and TRAF6 was blocked by ASK1 overexpression. It thus appears that the inhibition of NF-kappaB by ASK1 may result at least in part from the disruption of the TRAF6.TAK1 complex formation in the IL-1 signaling pathway. These results provide a new insight in the mode of action of MAPKKK family members; two distinct MAPKKKs in the same MAP kinase cascades directly interact and exert opposite effects in another signaling pathway, NF-kappaB.
Asunto(s)
Interleucina-1/farmacología , Quinasas Quinasa Quinasa PAM/metabolismo , FN-kappa B/metabolismo , Receptores de Interleucina-1/fisiología , Línea Celular , Humanos , Interleucina-1/fisiología , Proteínas Quinasas JNK Activadas por Mitógenos , Cinética , MAP Quinasa Quinasa Quinasa 5 , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Proteínas/metabolismo , Receptores de Interleucina-1/genética , Receptores Tipo I de Interleucina-1 , Proteínas Recombinantes/inmunología , Transducción de Señal/fisiología , Factor 6 Asociado a Receptor de TNF , Transfección , Proteínas Quinasas p38 Activadas por MitógenosRESUMEN
Apoptosis signal-regulating kinase 1 (ASK1) is a ubiquitously expressed mitogen-activated protein kinase kinase kinase that activates the c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase signaling cascades. We report here that expression of constitutively active ASK1 (ASK1DeltaN) induces neurite outgrowth in the rat pheochromocytoma cell line PC12. We found that p38 and to a lesser extent JNK, but not ERK, were activated by the expression of ASK1DeltaN in PC12 cells. ASK1DeltaN-induced neurite outgrowth was strongly inhibited by treatment with the p38 inhibitor SB203580 but not with the MEK inhibitors, suggesting that activation of p38, rather than of ERK, is required for the neurite-inducing activity of ASK1 in PC12 cells. We also observed that ASK1DeltaN induced expression of several neuron-specific proteins and phosphorylation of neurofilament proteins, confirming that PC12 cells differentiated into mature neuronal cells by ASK1. Moreover, ASK1DeltaN-expressing PC12 cells survived in serum-starved condition. ASK1 thus appears to mediate signals leading to both differentiation and survival of PC12 cells. Together with previous reports indicating that ASK1 functions as a pro-apoptotic signaling intermediate, these results suggest that ASK1 has a broad range of biological activities depending on cell types and/or cellular context.
Asunto(s)
Proteínas de Arabidopsis , Diferenciación Celular/fisiología , Supervivencia Celular/fisiología , Proteínas Quinasas Activadas por Mitógenos , Neuronas/citología , Proteínas de Plantas/fisiología , Animales , Proteínas Quinasas Dependientes de Calcio-Calmodulina/antagonistas & inhibidores , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Medio de Cultivo Libre de Suero , Activación Enzimática , Inhibidores Enzimáticos/farmacología , Imidazoles/farmacología , Neuritas , Células PC12 , Piridinas/farmacología , Ratas , Proteínas Quinasas p38 Activadas por MitógenosRESUMEN
The stress-activated protein kinases (SAPKs, also called c-Jun NH(2)-terminal kinases) and the p38s, two mitogen-activated protein kinase (MAPK) subgroups activated by cytokines of the tumor necrosis factor (TNF) family, are pivotal to the de novo gene expression elicited as part of the inflammatory response. Apoptosis signal-regulating kinase 1 (ASK1) is a MAPK kinase kinase (MAP3K) that activates both the SAPKs and p38s in vivo. Here we show that TNF receptor (TNFR) associated factor 2 (TRAF2), an adapter protein that couples TNFRs to the SAPKs and p38s, can activate ASK1 in vivo and can interact in vivo with the amino- and carboxyl-terminal noncatalytic domains of the ASK1 polypeptide. Expression of the amino-terminal noncatalytic domain of ASK1 can inhibit TNF and TRAF2 activation of SAPK. TNF can stimulate the production of reactive oxygen species (ROS), and the redox-sensing enzyme thioredoxin (Trx) is an endogenous inhibitor of ASK1. We also show that expression of TRAF2 fosters the production of ROS in transfected cells. We demonstrate that Trx significantly inhibits TRAF2 activation of SAPK and blocks the ASK1-TRAF2 interaction in a reaction reversed by oxidants. Finally, the mechanism of ASK1 activation involves, in part, homo-oligomerization. We show that expression of ASK1 with TRAF2 enhances in vivo ASK1 homo-oligomerization in a manner dependent, in part, upon the TRAF2 RING effector domain and the generation of ROS. Thus, activation of ASK1 by TNF requires the ROS-mediated dissociation of Trx possibly followed by the binding of TRAF2 and consequent ASK1 homo-oligomerization.
Asunto(s)
Quinasas Quinasa Quinasa PAM/metabolismo , Proteínas/metabolismo , Receptores del Factor de Necrosis Tumoral/metabolismo , Transducción de Señal , Tiorredoxinas/metabolismo , Factor de Necrosis Tumoral alfa/metabolismo , Línea Celular , Activación Enzimática , Regulación de la Expresión Génica , Humanos , MAP Quinasa Quinasa Quinasa 5 , Quinasas Quinasa Quinasa PAM/genética , Proteínas/genética , Receptores del Factor de Necrosis Tumoral/genética , Transducción de Señal/genética , Factor 2 Asociado a Receptor de TNF , Transfección , Factor de Necrosis Tumoral alfa/genéticaRESUMEN
Tumor necrosis factor (TNF)-induced activation of the c-jun N-terminal kinase (JNK, also known as SAPK; stress-activated protein kinase) requires TNF receptor-associated factor 2 (TRAF2). The apoptosis signal-regulating kinase 1 (ASK1) is activated by TNF and stimulates JNK activation. Here we show that ASK1 interacts with members of the TRAF family and is activated by TRAF2, TRAF5, and TRAF6 overexpression. A truncated derivative of TRAF2, which inhibits JNK activation by TNF, blocks TNF-induced ASK1 activation. A catalytically inactive mutant of ASK1 is a dominant-negative inhibitor of TNF- and TRAF2-induced JNK activation. In untransfected mammalian cells, ASK1 rapidly associates with TRAF2 in a TNF-dependent manner. Thus, ASK1 is a mediator of TRAF2-induced JNK activation.
Asunto(s)
Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Proteínas Quinasas Activadas por Mitógenos , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas/metabolismo , Animales , Apoptosis , Línea Celular , Activación Enzimática , Humanos , Proteínas Quinasas JNK Activadas por Mitógenos , Quinasas Quinasa Quinasa PAM , Mamíferos , Proteínas/genética , Receptores del Factor de Necrosis Tumoral/metabolismo , Proteínas Recombinantes/metabolismo , Transducción de Señal , Factor 2 Asociado a Receptor de TNF , Factor 5 Asociado a Receptor de TNF , Factor 6 Asociado a Receptor de TNF , TransfecciónRESUMEN
The Fas death receptor can activate the Jun NH2-terminal kinase (JNK) pathway through the receptor-associated protein Daxx. Daxx was found to activate the JNK kinase kinase ASK1, and overexpression of a kinase-deficient ASK1 mutant inhibited Fas- and Daxx-induced apoptosis and JNK activation. Fas activation induced Daxx to interact with ASK1, which consequently relieved an inhibitory intramolecular interaction between the amino- and carboxyl-termini of ASK1, activating its kinase activity. The Daxx-ASK1 connection completes a signaling pathway from a cell surface death receptor to kinase cascades that modulate nuclear transcription factors.
Asunto(s)
Apoptosis , Proteínas Portadoras/metabolismo , Péptidos y Proteínas de Señalización Intracelular , Proteínas Quinasas Activadas por Mitógenos , Proteínas Nucleares , Proteínas Serina-Treonina Quinasas/metabolismo , Proteínas Adaptadoras Transductoras de Señales , Alelos , Secuencia de Aminoácidos , Animales , Proteínas Quinasas Dependientes de Calcio-Calmodulina/metabolismo , Línea Celular , Proteínas Co-Represoras , Activación Enzimática , Humanos , Proteínas Quinasas JNK Activadas por Mitógenos , Quinasas Quinasa Quinasa PAM , Chaperonas Moleculares , Datos de Secuencia Molecular , Proteínas Serina-Treonina Quinasas/genética , Proteínas Recombinantes de Fusión/metabolismo , Transducción de Señal , Células Tumorales Cultivadas , Receptor fas/metabolismoRESUMEN
Apoptosis signal-regulating kinase (ASK) 1 was recently identified as a mitogen-activated protein (MAP) kinase kinase kinase which activates the c-Jun N-terminal kinase (JNK) and p38 MAP kinase pathways and is required for tumor necrosis factor (TNF)-alpha-induced apoptosis; however, the mechanism regulating ASK1 activity is unknown. Through genetic screening for ASK1-binding proteins, thioredoxin (Trx), a reduction/oxidation (redox)-regulatory protein thought to have anti-apoptotic effects, was identified as an interacting partner of ASK1. Trx associated with the N-terminal portion of ASK1 in vitro and in vivo. Expression of Trx inhibited ASK1 kinase activity and the subsequent ASK1-dependent apoptosis. Treatment of cells with N-acetyl-L-cysteine also inhibited serum withdrawal-, TNF-alpha- and hydrogen peroxide-induced activation of ASK1 as well as apoptosis. The interaction between Trx and ASK1 was found to be highly dependent on the redox status of Trx. Moreover, inhibition of Trx resulted in activation of endogenous ASK1 activity, suggesting that Trx is a physiological inhibitor of ASK1. The evidence that Trx is a negative regulator of ASK1 suggests possible mechanisms for redox regulation of the apoptosis signal transduction pathway as well as the effects of antioxidants against cytokine- and stress-induced apoptosis.
Asunto(s)
Apoptosis , Inhibidores Enzimáticos/farmacología , Proteínas Serina-Treonina Quinasas/metabolismo , Tiorredoxinas/metabolismo , Animales , Encéfalo/metabolismo , Línea Celular , Cloruros/farmacología , Medio de Cultivo Libre de Suero , Activación Enzimática , Humanos , Peróxido de Hidrógeno/farmacología , Células L , Quinasas Quinasa Quinasa PAM , Mamíferos , Ratones , Oxidación-Reducción , Proteínas Serina-Treonina Quinasas/antagonistas & inhibidores , Proteínas Serina-Treonina Quinasas/biosíntesis , Proteínas Recombinantes/antagonistas & inhibidores , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/metabolismo , Transducción de Señal , Tiorredoxinas/biosíntesis , Tiorredoxinas/farmacología , Transfección , Factor de Necrosis Tumoral alfa/farmacología , Compuestos de Zinc/farmacologíaRESUMEN
Bone morphogenetic proteins (BMPs)/osteogenic proteins (OPs), members of the transforming growth factor-beta superfamily, have a wide variety of effects on many cell types including osteoblasts and chondroblasts, and play critical roles in embryonic development. BMPs transduce their effects through binding to two different types of serine/threonine kinase receptors, type I and type II. Signaling by these receptors is mediated by the recently identified Smad proteins. Despite the rapid progress in understanding of the signaling mechanism downstream of BMP receptors, the target genes of BMPs are poorly understood in mammals. Here we identified a novel gene, termed BMP/OP-responsive gene (BORG), in C2C12 mouse myoblast cell line which trans-differentiates into osteoblastic cells in response to BMPs. Expression of BORG was dramatically induced in C2C12 cells by the treatment with BMP-2 or OP-1 within 2 h and peaked at 12-24 h, whereas transforming growth factor-beta had a minimal effect. BMP-dependent expression of BORG was also detected in other cell types which are known to respond to BMPs, suggesting that BORG is a common target gene of BMPs. Cloning and sequence analysis of BORG cDNA and the genomic clones revealed that, unexpectedly, the transcript of BORG lacks any extensive open reading frames and contains a cluster of multiple interspersed repetitive sequences in its middle part. The unusual structural features suggested that BORG may function as a noncoding RNA, although it is spliced and polyadenylated as authentic protein-coding mRNAs. Together with the observation that transfection of antisense oligonucleotides of BORG partially inhibited BMP-induced differentiation in C2C12 cells, it is possible that a new class of RNA molecules may have certain roles in the differentiation process induced by BMPs.
Asunto(s)
Proteínas Morfogenéticas Óseas/metabolismo , ARN/metabolismo , Animales , Secuencia de Bases , Northern Blotting , Línea Celular , Clonación Molecular , ADN Complementario , Exones , Intrones , Ratones , Ratones Endogámicos C3H , Datos de Secuencia Molecular , Oligonucleótidos Antisentido/farmacología , ARN/genéticaRESUMEN
Bone morphogenetic proteins (BMPs) are multifunctional cytokines, which induce bone and cartilage formation and exert various other effects on many tissues. Since angiogenesis is involved in the bone formation process, certain members in the BMP family may induce angiogenesis. We examined the in vivo angiogenic activity of BMP family members, i.e., growth/differentiation factor (GDF)-5 and BMP-2. GDF-5 induced angiogenesis in both chick chorioallantoic membrane and rabbit cornea assays. In contrast, BMP-2 did not induce angiogenesis. In order to elucidate the mechanism of angiogenesis, we examined the effects of GDF-5 on cultured bovine aortic endothelial cells (BECs). GDF-5 induced plasminogen activator activity and accelerated the migration of BECs in a chemotactic fashion, which may contribute to the process of angiogenesis in vivo. These results suggest that GDF-5 is one of the molecules which induce angiogenesis in the bone formation process.
Asunto(s)
Proteínas Morfogenéticas Óseas/farmacología , Córnea/irrigación sanguínea , Endotelio Vascular/fisiología , Sustancias de Crecimiento/farmacología , Neovascularización Fisiológica/efectos de los fármacos , Receptores de Factores de Crecimiento , Factor de Crecimiento Transformador beta/farmacología , Alantoides/irrigación sanguínea , Animales , Aorta , Proteína Morfogenética Ósea 2 , Receptores de Proteínas Morfogenéticas Óseas , Bovinos , División Celular/efectos de los fármacos , Movimiento Celular/efectos de los fármacos , Movimiento Celular/fisiología , Células Cultivadas , Embrión de Pollo , Corion/irrigación sanguínea , Endotelio Vascular/citología , Endotelio Vascular/efectos de los fármacos , Factor 5 de Diferenciación de Crecimiento , Humanos , Activadores Plasminogénicos/biosíntesis , Conejos , Receptores de Superficie Celular/biosíntesis , Proteínas Recombinantes/farmacologíaRESUMEN
Bone morphogenetic protein (BMP)-7/osteogenic protein (OP)-1 and growth/differentiation factor (GDF)-5 are members of the BMP family. BMPs transduce their effects through binding to two different types of serine/threonine kinase receptors, type I and type II. Here we investigated the binding and signaling properties of BMP-7/OP-1 and GDF-5 through type I and type II receptors. BMP-7/OP-1 was found to bind Activin receptor-like kinase (ALK)-1 as well as ALK-3/BMPR-IA in ATDC5 cells. When ALK-1 or ALK-3/BMPR-IA was stably transfected into mink lung epithelial cells, ALK-1 and ALK-3/BMPR-IA mediated signals for BMP-7/OP-1 with heterogeneous signaling specificities. GDF-5 bound to ALK-6/BMPR-IB and BMP type II receptor (BMPR-II) but not to ALK-3/BMPR-IA in ROB-C26 cells. Analysis using COS-1 cells revealed that GDF-5 bound to ALK-6/BMPR-IB, but not to the other type I receptors when expressed alone. When COS-1 cells were transfected with type II receptor cDNAs, GDF-5 bound to Activin type II receptor (ActR-II) and type IIB receptors as well as BMPR-II but not to TGF-beta type II receptor. In the presence of type II receptors, GDF-5 bound to different sets of type I receptors, but the binding was most efficient to ALK-6/BMPR-IB compared to the other type I receptors. Moreover, GDF-5 transduced the signal efficiently by ALK-6/BMPR-IB in the presence of BMPR-II or ActR-II.
Asunto(s)
Proteínas Morfogenéticas Óseas/fisiología , Receptores de Factores de Crecimiento/aislamiento & purificación , Animales , Células Cultivadas , Factor 5 de Diferenciación de Crecimiento , Sustancias de Crecimiento/fisiología , Ratones , Ratas , Transducción de Señal/fisiologíaRESUMEN
Growth/differentiation factor-5 (GDF-5) is a member of the bone morphogenetic protein (BMP) family, which plays an important role in bone development in vivo. Mutations in the GDF-5 gene result in brachypodism in mice and Hunter-Thompson type chondrodysplasia in human. BMPs transduce their effects through binding to two different types of serine/threonine kinase receptors, type I and type II. However, binding abilities appear to be different among the members of the BMP family. BMP-4 binds to two different type I receptors, BMP receptors type IA (BMPR-IA) and type IB (BMPR-IB), and a type II receptor, BMP receptor type II (BMPR-II). In addition to these receptors, osteogenic protein-1 (OP-1, also known as BMP-7) binds to activin type I receptor (ActR-I) as well as activin type II receptors (ActR-II and ActR-IIB). Here we investigate the binding and signaling properties of GDF-5 through type I and type II receptors. GDF-5 induced alkaline phosphatase activity in a rat osteoprogenitor-like cell line, ROB-C26. 125I-GDF-5 bound to BMPR-IB and BMPR-II but not to BMPR-IA in ROB-C26 cells and other nontransfected cell lines. Analysis using COS-1 cells transfected with the receptor cDNAs revealed that GDF-5 bound to BMPR-IB but not to the other type I receptors when expressed alone. When COS-1 cells were transfected with type II receptor cDNAs, GDF-5 bound to ActR-II, ActR-IIB, and BMPR-II but not to transforming growth factor-beta type II receptor. In the presence of type II receptors, GDF-5 bound to different sets of type I receptors, but the binding was most efficient to BMPR-IB compared with the other type I receptors. Moreover, a transcriptional activation signal was efficiently transduced by BMPR-IB in the presence of BMPR-II or ActR-II after stimulation by GDF-5. These results suggest that BMPR-IB mediates certain signals for GDF-5 after forming the heteromeric complex with BMPR-II or ActR-II.
Asunto(s)
Proteínas Morfogenéticas Óseas , Sustancias de Crecimiento/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Receptores de Superficie Celular/metabolismo , Receptores de Factores de Crecimiento , Fosfatasa Alcalina/metabolismo , Animales , Receptores de Proteínas Morfogenéticas Óseas , Receptores de Proteínas Morfogenéticas Óseas de Tipo 1 , Línea Celular , Factor 5 de Diferenciación de Crecimiento , Visón , Unión Proteica , Ratas , Transducción de SeñalRESUMEN
Proteins in the transforming growth factor-beta (TGF-beta) superfamily exert their effects by forming heteromeric complexes of their type I and type II serine/threonine kinase receptors. The type I and type II receptors form distinct subgroups in the serine/threonine kinase receptor family based on the sequences of the kinase domains and the presence of a highly conserved region called the GS domain (or type I box) located just N-terminal to the kinase domain in the type I receptors. Recent studies have revealed that upon TGF-beta binding several serine and threonine residues in the GS domain of TGF-beta type I receptor (T beta R-I) are phosphorylated by TGF-beta type II receptor (T beta R-II) and that the phosphorylation of GS domain is essential for TGF-beta signaling. Here we investigated the role of cytoplasmic juxtamembrane region located between the transmembrane domain and the GS domain of T beta R-I by mutational analyses using mutant mink lung epithelial cells, which lack endogenous T beta R-I. Upon transfection, wild-type T beta R-I restored the TGF-beta signals for growth inhibition and production of plasminogen activator inhibitor-1 (PAI-1) and fibronectin. A deletion mutant, T beta R-I/JD1(delta 150-181), which lacks the juxtamembrane region preceding the GS domain, bound TGF-beta in concert with T beta R-II and transduced a signal leading to production of PAI-I but not growth inhibition. Recombinant receptors with mutations that change serine 172 to alanine (S172A) or threonine 176 to valine (T176V) were similar to wild-type T beta R-I in their abilities to bind TGF-beta, formed complexes with T beta R-II, and transduced a signal for PAI-1 and fibronectin. Similar to T beta R-I/JD1 (delta 150-181), however, these missence mutant receptors were impaired to mediate a growth inhibitory signal. These observations indicate that serine 172 and threonine 176 of T beta R-I are dispensable for extracellular matrix protein production but essential to the growth inhibition by TGF-beta.