Recent progress on nickel phthalocyanine-based electrocatalysts for CO2 reduction.

The electrocatalytic reduction of CO2 to high-value fuels by renewable electricity is a sustainable strategy, which can substitute for fossil fuels and circumvent climate changes induced by elevated CO2 emission levels, making the rational design of versatile electrocatalysts highly desirable. Among all the electrocatalytic materials used in the CO2 reduction reaction, nickel phthalocyanine (NiPc)-based electrocatalysts have attracted considerable attention recently because of their high CO selectivity and catalytic activity. Herein, we review the latest advances in CO2 electroreduction to CO catalyzed by immobilized NiPc and its derivatives on diverse surfaces. Specific strategies, the structure-performance relationship and the CO2-to-CO reaction mechanism of these NiPc-based electrocatalysts are analyzed. Future opportunities and challenges for this series of powerful heterogeneous electrocatalysts are also highlighted.

Predicting residual friction angle of lunar regolith based on Chang'e-5 lunar samples.

With the rapid development of human lunar exploration projects, the lunar base establishment and resource utilization are on the way, and hence it is urgent and significant to reasonably predict engineering properties of the lunar regolith, which remains to be unclear due to limited lunar samples currently accessible for geotechnical tests. In this contribution, we aim to address this outstanding challenge from the perspective of granular material mechanics. To this end, the 3D multi-aspect geometrical characteristics and mechanical properties of Chang'e-5 lunar samples are for the first time evaluated with a series of non-destructive microscopic tests. Based on the measured particle surface roughness and Young's modulus, the interparticle friction coefficients of lunar regolith particles are well predicted through an experimental fitting approach using previously published data on terrestrial geomaterials or engineering materials. Then the residual friction angle of the lunar regolith under low confining pressure is predicted as 53° to 56° according to the particle overall regularity and interparticle friction coefficients of Chang'e-5 lunar samples. The presented results provide a novel cross-scale method to predict engineering properties of the lunar regolith from particle scale information to serve for the future lunar surface engineering construction.

Characteristic of water quality indicators and its response to climate conditions in the middle and lower reaches of Lijiang River, China.

With global climate change and increasingly extreme weather conditions, the water quality of the Lijiang River Basin (LRB) is facing huge threats. At present, there is still a lack of systematic research on water quality indicators and the influence of indirect factors such as meteorological factors on it in the LRB. Therefore, this study is based on the meteorological, hydrological, and water quality data of the LRB from 2012 to 2018, using the Mann-Kendall test, Morlet wavelet method, Spearman's rank correlation coefficient, sensitivity, and contribution rate to quantitative analysis of the relationship between climate conditions and water quality indicators. The results show that the change trends of these hydrological and climatic conditions have almost no significant sudden change; precipitation and streamflow are decreasing each year; the streamflow trend exhibits time hysteresis; precipitation has a stronger influence downstream than on the local area; water quality indicators of both stations exhibited a change period of around 18 to 20 months, with the exception of pH. Water quality indicators are insensitive to precipitation and streamflow, and sensitive to humidity and wind speed; DO was negatively correlated with climate indicators apart from wind speed; almost all water quality indicators in Yangshuo are highly sensitive to air temperature, and the contribution rate of air temperature to ORP and TP reached 4.81% and 3.56%, respectively; sunshine duration has a positive impact on reducing NH4-N and TP. The difference between Yangshuo and Guilin is mostly due to the input of external sources on both sides of the Lijiang River, which results in variations in climate conditions sensitivities.

Hydrogeochemical characteristics and processes of thermokarst lake and groundwater during the melting of the active layer in a permafrost region of the Qinghai-Tibet Plateau, China.

Permafrost degradation and the development of thermokarst lakes are important factors driving the variability of regional hydrologic processes. Hydrogeochemical and isotopic analyses are important methods for investigating the hydrologic processes of thermokarst lakes. This study focused on comparing the chemical and hydrogeochemical characteristics between lake water and groundwater during the melting of the active layer in a typical thermokarst lake region on the Qinghai-Tibet Plateau (QTP). Ninety-five samples were collected during different periods of active layer melting and analyzed using statistical, isotope, hydrogeochemical, and modeling methods. Statistical results showed that the average concentrations of almost all ions were lower in lake water than in groundwater, with wider spatial variability in groundwater. The lake water is of the ClNa and HCO3-Ca type with low TDS (total dissolved solids), whereas groundwater is of the HCO3-Ca and mixed type (or transition type) with high TDS. The chemical types of the lake water and groundwater are mainly driven by rock weathering. In terms of the saturation index (SI), halite and gypsum are unsaturated dissolved, whereas dolomite and calcite are generally saturated. Evaporation significantly affects the chemical composition of groundwater, while the hydrochemical compositions of lake water are relatively stable under the joint control of evaporation, precipitation, surface runoff, and groundwater. The isotopic analysis results showed that the contribution of permafrost meltwater and precipitation to groundwater and lake water varied during different stages of active layer melting. According to hydrogeochemical modeling, the main chemical reactions in groundwater are the precipitation of calcite and the dissolution of halite, dolomite, and gypsum. The intensity of groundwater flow determines the degree of chemical reactions along the flow path at different stages of active layer melting. The findings can provide deeper insight into hydrogeochemical processes in thermokarst lake regions under the background of permafrost degradation.

Effect of Calcium Carbide Residue on Strength Development along with Mechanisms of Cement-Stabilized Dredged Sludge.

The purpose of this research is to explore the feasibility of using calcium carbide residue (CCR), a by-product from acetylene gas production, as a solid alkaline activator on the strength development in CCR-Portland cement-stabilized dredged sludge (CPDS). The effects of cement content, CCR content and curing time on the strength development of CPDS were investigated using a series of unconfined compressive strength (UCS), pH and electric conductivity (EC) tests. Scanning electron microscopy and X-ray diffraction analyses were performed to gain additional insight into the mechanism of strength development. Meanwhile, the carbon footprints of CPDS were calculated. Following the results, it was found that CCR can significantly improve the strength of cemented dredged sludge. On the basis of the strength difference (ΔUCS) and strength growth rate (UCSgr), it was recommended that utilizing 20% cement with the addition of 20% CCR is the most effective way to develop the long-term strength of CPDS. In addition, the microstructural analysis verified that the optimum proportion of CCR benefits the formation of hydration products in CPDS, particularly needle-like gel ettringite, resulting in a less-porous and dense inter-locked structure. Furthermore, the solidification mechanism of CPDS was discussed and revealed. Finally, it was confirmed that CCR can be a sustainable alternative and effective green alkaline activator for the aim of improving cemented dredged sludge.

Temporal and Spatial Variation Characteristics of Water Quality in the Middle and Lower Reaches of the Lijiang River, China and Their Responses to Environmental Factors.

As the climate and the external environment have changed, the environmental factors of the Lijiang River Basin (LRB) have changed, posing new threats to the environmental quality, ecosystem balance, and management and protection of the water environment of the Lijiang River. Water quality indicators and environmental factors vary spatially along the Lijiang River, which runs through urban areas, farmland, and karst areas. However, research on the response of water quality to water environmental factors is still lacking. Within this context, this study considered statistical methods and hydrological, meteorological, and water quality data of the middle and lower reaches of the Lijiang River from 2012 to 2018, expounded on the temporal and spatial change characteristics and evolution trends of water quality indicators; we analyzed the correlation between water quality indicators and environmental factors; we quantitatively assessed the sensitivity and contribution rate of water quality indicators to environmental factors. The results demonstrated that rainfall feedback on the river streamflow was lagging, and upstream precipitation often affected downstream streamflow. The water quality in the upper reaches of Guilin has improved year by year, and pollution levels have increased slightly when flowing through the urban area of Guilin. In spite of this, it still falls within the range of self-purification. River characteristics heavily influence the impact of environmental factors on water quality indicators; in contrast, the effects of different locations along the same river are more similar. Four water quality indicators are negatively correlated with water temperature, pH, and dissolved oxygen (DO). The sensitivities of ammonia nitrogen (NH4-N) and chemical oxygen demand (CODMn) to streamflow increase with the flow direction. The contribution rates of DO-to-total phosphorus (TP) and pH-to-TP are over -6%. Water temperature is the major contributing factor in the Lijiang River, while DO has a higher contribution in tributaries. The external sources affect the concentration of various water quality indicators and the sensitivity of water quality indicators to the external environment. There should be a series of measures implemented to reduce pollution, such as using oxygenation or chemical means to increase pH in Dahe and Yangshuo to control water pollutants. Tourism and particular karst topography make LRB's calculations unique, but the research method can be applied to other watersheds as well.

A Semiconductor-Mediator-Catalyst Artificial Photosynthetic System for Photoelectrochemical Water Oxidation.

In fabricating an artificial photosynthesis (AP) electrode for water oxidation, we have devised a semiconductor-mediator-catalyst structure that mimics photosystem II (PSII). It is based on a surface layer of vertically grown nanorods of Fe2 O3 on fluorine doped tin oxide (FTO) electrodes with a carbazole mediator base and a Ru(II) carbene complex on a nanolayer of TiO2 as a water oxidation co-catalyst. The resulting hybrid assembly, FTO|Fe2 O3 |-carbazole|TiO2 |-Ru(carbene), demonstrates an enhanced photoelectrochemical (PEC) water oxidation performance compared to an electrode without the added carbaozle base with an increase in photocurrent density of 2.2-fold at 0.95 V vs. NHE and a negatively shifted onset potential of 500 mV. The enhanced PEC performance is attributable to carbazole mediator accelerated interfacial hole transfer from Fe2 O3 to the Ru(II) carbene co-catalyst, with an improved effective surface area for the water oxidation reaction and reduced charge transfer resistance.

Ice needles weave patterns of stones in freezing landscapes.

Patterned ground, defined by the segregation of stones in soil according to size, is one of the most strikingly self-organized characteristics of polar and high-alpine landscapes. The presence of such patterns on Mars has been proposed as evidence for the past presence of surface liquid water. Despite their ubiquity, the dearth of quantitative field data on the patterns and their slow dynamics have hindered fundamental understanding of the pattern formation mechanisms. Here, we use laboratory experiments to show that stone transport is strongly dependent on local stone concentration and the height of ice needles, leading effectively to pattern formation driven by needle ice activity. Through numerical simulations, theory, and experiments, we show that the nonlinear amplification of long wavelength instabilities leads to self-similar dynamics that resemble phase separation patterns in binary alloys, characterized by scaling laws and spatial structure formation. Our results illustrate insights to be gained into patterns in landscapes by viewing the pattern formation through the lens of phase separation. Moreover, they may help interpret spatial structures that arise on diverse planetary landscapes, including ground patterns recently examined using the rover Curiosity on Mars.

A stable dye-sensitized photoelectrosynthesis cell mediated by a NiO overlayer for water oxidation.

In the development of photoelectrochemical cells for water splitting or CO2 reduction, a major challenge is O2 evolution at photoelectrodes that, in behavior, mimic photosystem II. At an appropriate semiconductor electrode, a water oxidation catalyst must be integrated with a visible light absorber in a stable half-cell configuration. Here, we describe an electrode consisting of a light absorber, an intermediate electron donor layer, and a water oxidation catalyst for sustained light driven water oxidation catalysis. In assembling the electrode on nanoparticle SnO2/TiO2 electrodes, a Ru(II) polypyridyl complex was used as the light absorber, NiO was deposited as an overlayer, and a Ru(II) 2,2'-bipyridine-6,6'-dicarboxylate complex as the water oxidation catalyst. In the final electrode, addition of the NiO overlayer enhanced performance toward water oxidation with the final electrode operating with a 1.1 mA/cm2 photocurrent density for 2 h without decomposition under one sun illumination in a pH 4.65 solution. We attribute the enhanced performance to the role of NiO as an electron transfer mediator between the light absorber and the catalyst.

Freezing/thawing index variations over the circum-Arctic from 1901 to 2015 and the permafrost extent.

Due to sparse data and discontinuous time observations in the circum-Arctic region, freezing index and thawing index, as useful indicators, are widely used in permafrost distribution, climate changes and cold-region engineering analysis. However, previous researches on freezing/thawing index over this region were estimated based on mean monthly air temperature. In this paper, we analyzed the spatial and temporal variations of the freezing/thawing index over the circum-Arctic from 1901 to 2015 based on the daily datasets, besides monthly datasets. The results showed that freezing index had a downward changing trend and thawing index had an upward trend during 1901-2015. More important, the change trend in freezing/thawing index after 1988 was more significant than before. Furthermore, different freezing/thawing index based on daily datasets and the monthly datasets were assessed and compared according to daily data from 17 meteorological stations, comprehensive relative errors evaluation implied that freezing/thawing based on daily datasets was more accurate generally, although both of other datasets were available in calculating the freezing/thawing index. As the daily datasets are better in calculating annual freezing/thawing index, therefore, the permafrost extent was estimated by a climate-based predictive model combined with snow depth data from Canadian Meteorological Centre (CMC). Finally, considering that the published permafrost map of the circum-Arctic only shows the past permafrost distribution, but it cannot reflect the permafrost distribution after 2000 under the climate warming. Hence, we simulated the current (mean from 2000 to 2015) permafrost area which is 19.96 × 106 km2, and the results showed some discrepancies between published and simulated permafrost extent mainly located in isolated permafrost regions.

Evaluation of thermokarst lake water balance in the Qinghai-Tibet Plateau via isotope tracers.

Thermokarst lakes are a ubiquitous landscape feature, which widely distributed in the pan-arctic and some low latitude regions, and are associated with regional hydrological processes. The studies were taken to obtain a better understanding of the water balance of thermokarst lakes in the Qinghai-Tibet Plateau (QTP) in order to gain insight of the regional hydrological cycle. The characteristics of the stable isotopes δ 18O and δ D were investigated in precipitation, permafrost meltwater, and thermokarst lake water in the continuous permafrost region of the QTP and analyzed the lake water balance using the isotope mass model. The results showed that the δ D-δ 18O relationship in the thermokarst lakes (δ D = 5.45 δ 18O - 18.95) differed from that of the local precipitation (δ D = 8.30 δ 18O + 18.49) and permafrost meltwater (δ D = 5.78 δ 18O - 23.41), and the mean isotope compositions in the thermokarst lakes were -7.2‰ in δ 18O and -58.0‰ in δ D. The more positive isotope signals in thermokarst lakes than in the precipitation and permafrost meltwater revealed that the lakes had experienced stronger isotope enrichment. Additionally, the evaporation-to-inflow ratio (E/I) values were < 1 in most of the thermokarst lakes (84%), which might be explained by the recent expansion of the lake surfaces. However, 16% of the thermokarst lakes had shrunk, owing to thermokarst erosion, lateral expansion as the temperature increases, and lower recharge volume. Moreover, precipitation on the lake surface was only 14-18% of the inflow volume in the thermokarst lakes, and the surface-subsurface inflow and permafrost meltwater are very important for recharging the lakes and maintaining the water balance. The results of this study provide a comprehensive understanding of the influence of climate warming on hydrological processes in the permafrost regions in the QTP.

Effects of local factors and climate on permafrost conditions and distribution in Beiluhe basin, Qinghai-Tibet Plateau, China.

Beiluhe basin is underlain by warm and ice-rich permafrost, and covered by vegetation and soils characteristic of the Qinghai-Tibet Plateau. A field monitoring network was established to investigate permafrost conditions and to assess potential impacts of local factors and climate change. This paper describes the spatial variations in permafrost conditions from instrumented boreholes, controlling environmental factors, and recent thermal evolution of permafrost in the basin. The study area was divided into 10 ecotypes using satellite imagery based classification. The field investigations and cluster analysis of ground temperatures indicated that permafrost underlies most of the ground in swamp meadow, undisturbed alpine meadow, degrading alpine meadow, and desert alpine grassland, but is absent in other cover types. Permafrost-ecotope relations examined over a 2-year (2014-2016) period indicated that: (i) ground surface temperatures varied largely among ecotopes; (ii) annual mean ground temperatures ranged from -1.5 to 0°C in permafrost, indicating sensitive permafrost conditions; (iii) active-layer thicknesses ranged from 1.4m to 3.4m; (iv) ground ice content at the top of permafrost is high, but the active-layer soil is relatively dry. Long-term climate warming has driven thermal changes to permafrost, but ground surface characteristics and soil moisture content strongly influence the ground thermal state. These factors control local-scale spatial variations in permafrost conditions. The warm permafrost in the basin is commonly in thermal disequilibrium, and is sensitive to future climate change. Active-layer thicknesses have increased by at least 42cm and the mean annual ground temperatures have increased by up to 0.2°C in the past 10years over the basin. A permafrost distribution map was produced based on ecotypes, suggesting that permafrost underlies 64% of the study region.

Impact of a thermokarst lake on the soil hydrological properties in permafrost regions of the Qinghai-Tibet Plateau, China.

The formation of thermokarst lakes can degrade alpine meadow ecosystems through changes in soil water and heat properties, which might have an effect on the regional surface water and groundwater processes. In this study, a typical thermokarst lake was selected in the Qinghai-Tibet Plateau (QTP), and the ecological index (SL) was used to divide the affected areas into extremely affected, severely affected, medium-affected, lightly affected, and non-affected areas, and soil hydrological properties, including saturated hydraulic conductivity and soil water-holding capacity, were investigated. The results showed that the formation of a thermokarst lake can lead to the degradation of alpine meadows, accompanied by a change in the soil physiochemical and hydrological properties. Specifically, the soil structure turned towards loose soil and the soil nutrients decreased from non-affected areas to severely affected areas, but the soil organic matter and available potassium increased slightly in the extremely affected areas. Soil saturated hydraulic conductivity showed a 1.7- to 4.1-fold increase in the lake-surrounding areas, and the highest value (401.9cmd-1) was detected in the severely affected area. Soil water-holding capacity decreased gradually during the transition from the non-affected areas to the severely affected areas, but it increased slightly in the extremely affected areas. The principal component analysis showed that the plant biomass was vital to the changes in soil hydrological properties. Thus, the vegetation might serve as a link between the thermokarst lake and soil hydrological properties. In this particular case, it was concluded that the thermokarst lake adversely affected the regional hydrological services in the alpine ecosystem. These results would be useful for describing appropriate hydraulic parameters with the purpose of modeling soil water transportation more accurately in the Qinghai-Tibet Plateau.

Enhanced photoelectrochemical water oxidation of bismuth vanadate via a combined strategy of W doping and surface RGO modification.

Nanoporous bismuth vanadate is modified simultaneously via tungsten doping and graphene surface modification for use as an efficient photoanode. The modified films were prepared on a FTO substrate by a drop-cast method followed by photoreduction of graphene oxide. SEM, XRD, Raman and XPS characterization was conducted to confirm the incorporation of tungsten and reduced graphene oxide (RGO), and to look into their influences on the structure and performance of BiVO4. Electrochemical impedance spectroscopy analysis clearly revealed enhanced carrier density and improved electronic conductivity, which are beneficial for the enhancement of PEC performance in comparison to either individually doped or RGO modified BiVO4. Our results indicated that the enhanced PEC performance can be attributed to the synergistic effect of bulk doping and surface modification that facilitates electron and hole transport and transfer in the bulk and at the semiconductor-electrolyte interface.

A naturally-occurring 'cold earth' spot in Northern China.

Permafrost is determined to a large extent by the Earth's surface temperature, therefore it distributes mainly in high altitude and latitude regions. However, stable, warm (about -1 °C) permafrost occurs within a scree slope in northern China that is more than 600 km south of the southernmost limit of latitudinal permafrost on the Eurasian Continent. It is at an elevation of only 900 m above sea level (ASL). The area has a mean annual air temperature (MAAT) of 6 to 8 °C. Thermal processes of the scree slope, investigated through field monitoring and numerical simulation, showed that the permafrost is caused by winter air convection within the porous rock deposits and is stable as air convection does not occur in summer time. The deposit is covered by a 30-cm-thick peaty soil layer dated (carbon C-14) to between 1,000 to 1,600 years ago. The layer also contributes to the permafrost's existence due to the peat's thermal conductivity offset when frozen and thawed. The existence of permafrost under such warm climatic conditions confirms the effectiveness of using crushed rock layer as basement or slope cover to protect the warm permafrost subgrade of the recently-constructed Qinghai-Tibet Railway (QTR), even under the predicted climate warming conditions.

Characterization of the prokaryotic diversity through a stratigraphic permafrost core profile from the Qinghai-Tibet Plateau.

Permafrost on the Qinghai-Tibet Plateau is one of the most sensitive regions to climate warming, thus characterizing its microbial diversity and community composition may be important for understanding their potential responses to climate changes. Here, we investigated the prokaryotic diversity in a 10-m-long permafrost core from the Qinghai-Tibet Plateau by restriction fragment length polymorphism analysis targeting the 16S rRNA gene. We detected 191 and 17 bacterial and archaeal phylotypes representing 14 and 2 distinct phyla, respectively. Proteobacteria was the dominant bacterial phylum, while archaeal communities were characterized by a preponderance of Thaumarchaeota. Some of prokaryotic phylotypes were closely related to characterized species involved in carbon and nitrogen cycles, including nitrogen fixation, methane oxidation and nitrification. However, the majority of the phylotypes were only distantly related to known taxa at order or species level, suggesting the potential of novel diversity. Additionally, both bacterial α diversity and community composition changed significantly with sampling depth, where these communities mainly distributed according to core horizons. Arthrobacter-related phylotypes presented at high relative abundance in two active layer soils, while the deeper permafrost soils were dominated by Psychrobacter-related clones. Changes in bacterial community composition were correlated with most measured soil variables, such as carbon and nitrogen contents, pH, and conductivity.

Relative Roles of Deterministic and Stochastic Processes in Driving the Vertical Distribution of Bacterial Communities in a Permafrost Core from the Qinghai-Tibet Plateau, China.

Understanding the processes that influence the structure of biotic communities is one of the major ecological topics, and both stochastic and deterministic processes are expected to be at work simultaneously in most communities. Here, we investigated the vertical distribution patterns of bacterial communities in a 10-m-long soil core taken within permafrost of the Qinghai-Tibet Plateau. To get a better understanding of the forces that govern these patterns, we examined the diversity and structure of bacterial communities, and the change in community composition along the vertical distance (spatial turnover) from both taxonomic and phylogenetic perspectives. Measures of taxonomic and phylogenetic beta diversity revealed that bacterial community composition changed continuously along the soil core, and showed a vertical distance-decay relationship. Multiple stepwise regression analysis suggested that bacterial alpha diversity and phylogenetic structure were strongly correlated with soil conductivity and pH but weakly correlated with depth. There was evidence that deterministic and stochastic processes collectively drived bacterial vertically-structured pattern. Bacterial communities in five soil horizons (two originated from the active layer and three from permafrost) of the permafrost core were phylogenetically random, indicator of stochastic processes. However, we found a stronger effect of deterministic processes related to soil pH, conductivity, and organic carbon content that were structuring the bacterial communities. We therefore conclude that the vertical distribution of bacterial communities was governed primarily by deterministic ecological selection, although stochastic processes were also at work. Furthermore, the strong impact of environmental conditions (for example, soil physicochemical parameters and seasonal freeze-thaw cycles) on these communities underlines the sensitivity of permafrost microorganisms to climate change and potentially subsequent permafrost thaw.

A cocatalyst-free Eosin Y-sensitized p-type of Co3O4 quantum dot for highly efficient and stable visible-light-driven water reduction and hydrogen production.

Owing to the effect of energy band bending, p-type Co3O4 quantum dots sensitized by Eosin Y showed a high and stable photocatalytic activity (∼13,440 µmol h(-1) g(-1)(cat)) for water reduction and hydrogen production under visible-light irradiation without any cocatalyst.

Effects of enhanced UV-B radiation on the diversity and activity of soil microorganism of alpine meadow ecosystem in Qinghai-Tibet Plateau.

The effects of enhanced UV-B radiation on abundance, community composition and the total microbial activity of soil bacteria in alpine meadow ecosystem of Qinghai-Tibet Plateau were investigated. Traditional counting and 16S rRNA gene sequencing were used to investigate the culturable bacteria and their composition in soil, meanwhile the total microbial activity was measured by microcalorimetry. The population of soil culturable bacteria was slightly reduced with the enhanced UV-B radiation in both of the two depths, 2.46 × 10(6) CFU/g in upper layer (0-10 cm), 1.44 × 10(6) CFU/g in under layer (10-20 cm), comparing with the control (2.94 × 10(6) CFU/g in upper layer, 1.65 × 10(6) CFU/g in under layer), although the difference was not statistically significant (P > 0.05). However, the bacteria diversity decreased obviously due to enhanced UV-B, the number of species for upper layer was decreased from 20 to 13, and from 16 to 13 for the lower layer. The distribution of species was also quite different between the two layers. Another obvious decrease induced by enhanced UV-B radiation was in the total soil microbial activities, which was represented by the microbial growth rate constant (k) in this study. The results indicated that the culturable bacteria community composition and the total activity of soil microbes have been considerably changed by the enhanced UV-B radiation.

Diversity and community structure of fungi through a permafrost core profile from the Qinghai-Tibet Plateau of China.

While a vast number of studies have addressed the prokaryotic diversity in permafrost, characterized by subzero temperatures, low water activity, and extremely low rates of nutrient and metabolite transfer, fungal patterns have received surprisingly limited attention. Here, the fungal diversity and community structure were investigated by culture-dependent technique combined with cloning-restriction fragment length polymorphism (RFLP) analysis of sediments in a 10-m-long permafrost core from the Qinghai-Tibet Plateau of China. A total of 62 fungal phylotypes related to 10 distinct classes representing three phyla were recovered from 5031 clones generated in 13 environmental gene libraries. A large proportion of the phylotypes (25/62) that were distantly related to described fungal species appeared to be novel diversity. Ascomycota was the predominant group of fungi, with respect to both clone and phylotype number. Our results suggested there was the existence of cosmopolitan psychrophilic or psychrotolerant fungi in permafrost sediments, the community composition of fungi varied with increasing depth, while these communities largely distributed according to core layers.