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Lateral flow immunoassay (LFIA) is extensively utilized for point-of-care testing due to its ease of operation, cost-effectiveness, and swift results. This study investigates the flow dynamics and reaction mechanisms in LFIA by developing a three-dimensional model using the Richards equation and porous media transport, and employing numerical simulations through the finite element method. The study delves into the transport and diffusion behaviors of each reaction component in both sandwich LFIA and competitive LFIA under non-uniform flow conditions. Additionally, the impact of various parameters (such as reporter particle concentration, initial capture probe concentrations for the T-line and C-line, and reaction rate constants) on LFIA performance is analyzed. The findings reveal that, in sandwich LFIA, optimizing parameters like increasing reporter particle concentration and initial capture probe concentration for the T-line, as well as adjusting reaction rate constants, can effectively enhance detection sensitivity and broaden the working range. Conversely, in competitive LFIA, the effects are inverse. This model offers valuable insights for the design and enhancement of LFIA assays.
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Nanopartículas del Metal , Inmunoensayo/métodosRESUMEN
The key to sEMG (surface electromyography)-based control of robotic hands is the utilization of sEMG signals from the affected hand of amputees to infer their motion intentions. With the advancements in deep learning, researchers have successfully developed viable solutions for CNN (Convolutional Neural Network)-based gesture recognition. However, most studies have primarily concentrated on utilizing sEMG data from the hands of healthy subjects, often relying on high-dimensional feature vectors obtained from a substantial number of electrodes. This approach has yielded high-performing sEMG recognition systems but has failed to consider the considerable inconvenience that the abundance of electrodes poses to the daily lives and work of patients. In this paper, we focused on transradial amputees and used sEMG data from the Ninapro DB3 database as our dataset. Firstly, we introduce a STFT (Short-Time Fourier Transform)-based time-frequency feature fusion map for sEMG. This map includes both time-frequency features and the time-frequency localization of sEMG signals. Secondly, we propose an Improved DenseNet (Dense Convolutional Network) model for recognizing motion intentions in the affected hand of amputees based on their sEMG signals. Finally, addressing the issue of optimizing the number of electrodes carried by amputees, we introduce the PCMIRR (Pearson Correlation and Motion Intention Recognition Rate) algorithm. This algorithm optimizes the number of channels by considering the Pearson correlation between the sEMG channels of amputees and the recognition rate of motion intentions in the affected hand based on single-channel sEMG data. The experimental results reveal that the recognition accuracy, recall, and F1 score achieved by the Improved DenseNet model were 93.82%, 93.61%, and 93.65%, respectively. When the number of electrodes was optimized to 8, the recognition accuracy reached 94.50%. In summary, this paper ultimately attained precise recognition of motion intentions in amputees' affected hands while utilizing the minimum number of sEMG channels. This method offers a novel approach to sEMG-based control of bionic robotic hands.
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Grading dried shiitake mushrooms is an indispensable production step, as there are large quality differences between different grades, which affect the product's price and marketability. Dried shiitake mushroom samples have irregular shapes, small morphological differences between different grades of the same species, and they may occur in mixed grades, which causes challenges to the automatic grade recognition using machine vision. In this study, a comprehensive method to solve this problem is provided, including image acquisition, preprocessing, dataset creation, and grade recognition. The osprey optimization algorithm (OOA) is used to improve the computational efficiency of Otsu's threshold binarization and obtain complete mushroom contours samples efficiently. Then, a method for dried shiitake mushroom grade recognition based on the improved VGG network (D-VGG) is proposed. The method uses the VGG16 network as the base framework, optimizes the convolutional layer of the network, and uses a global average pooling layer instead of a fully connected layer to reduce the risk of model overfitting. In addition, a residual module and batch normalization are introduced to enhance the learning effect of texture details, accelerate the convergence of the model, and improve the stability of the training process. An improved channel attention network is proposed to enhance the feature weights of different channels and improve the grading performance of the model. The experimental results show that the improved network model (D-VGG) can recognize different dried shiitake mushroom grades with high accuracy and recognition efficiency, achieving a final grading accuracy of 96.21%, with only 46.77 ms required to process a single image. The dried shiitake mushroom grade recognition method proposed in this study provides a new implementation approach for the dried shiitake mushroom quality grading process, as well as a reference for real-time grade recognition of other agricultural products.
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Position determination is a critical technical challenge to be addressed in the unmanned and intelligent advancement of crane systems. Traditional positioning techniques, such as those based on magnetic grating or encoders, are limited to measuring the positions of the main carriage and trolley. However, during crane operations, accurately determining the position of the load becomes problematic when it undergoes swinging motions. To overcome this limitation, this paper proposes a novel Ultra-Wide-Band (UWB) positioning method for unmanned crane systems, leveraging the Snake Optimizer Long Short-Term Memory (SO-LSTM) framework. The objective is to achieve real-time and precise localization of the crane hook. The proposed method establishes a multi-base station and multi-tag UWB positioning system using a Time Division Multiple Access (TDMA) combined with Two-Way Ranging (TWR) scheme. This system enables the acquisition of distance measurements between the mobile tag and UWB base stations. Furthermore, the hyperparameters of the LSTM network are optimized using the Snake Optimizer algorithm to enhance the accuracy and effectiveness of UWB positioning estimation. Experimental results demonstrate that the SO-LSTM-based positioning method yields a maximum positioning error of 0.1125 meters and a root mean square error of 0.0589 meters. In comparison to conventional approaches such as the least squares method (LS) and the Kalman filter method (KF), the proposed SO-LSTM-based positioning method significantly reduces the root mean square error (RMSE) by 63.39% and 58.01%, respectively, while also decreasing the maximum positioning error (MPE) by 60.77% and 52.65%.
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Algoritmos , Camillas , Inteligencia , Memoria a Largo Plazo , Movimiento (Física)RESUMEN
The unique infinite self-renewal ability and multidirectional differentiation potential of stem cells provide a strong support for the clinical treatment. In light of the growing demands for stem cell storage, how to ensure personal privacy security and comply with strict ethical supervision requirements is particularly important. In order to solve the problem of low security of traditional encryption algorithm, we proposed a double encryption protection (DEP) algorithm for stem cell bank privacy data based on improved AES and chaotic encryption technology. Firstly, we presented the hash value key decomposition algorithm, through the hash value dynamic coding, cyclic shift, conversion calculation to get the key of each subsystem in the built algorithm. Secondly, DEP algorithm for privacy data is realized with two level of encryption. The first level of encryption protection algorithm used AES as the main framework, adding dynamic coding and byte filling based on DNA coding, and carries out dynamic shift of rows and simplified mixing of columns. The second level of encryption protection algorithm conducted random encoding, operation, diffusion and decoding based on the results of our proposed sequence conversion algorithm. Finally, we raised two evaluation indexes, the number of characters change rate (NCCR) and the unified average change intensity of text (UACIT) to measure the sensitivity of encryption algorithms to changes in plain information. The experimental results of using DEP shown that the average values of histogram variance, information entropy, NCCR and UACIT are116.7883, 7.6688, 32.52% and 99.67%, respectively. DEP algorithm has a large key space, high key sensitivity, and enables dynamic encryption of private data in stem cell bank. The encryption scheme provided in this study ensures the security of the private information of stem cell bank in private cloud environment, and also provides a new method for the encryption of similar high confidentiality data.
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Seguridad Computacional , Privacidad , Algoritmos , Confidencialidad , ADNRESUMEN
Detection of the four tobacco shred varieties and the subsequent unbroken tobacco shred rate are the primary tasks in cigarette inspection lines. It is especially critical to identify both single and overlapped tobacco shreds at one time, that is, fast blended tobacco shred detection based on multiple targets. However, it is difficult to classify tiny single tobacco shreds with complex morphological characteristics, not to mention classifying tobacco shreds with 24 types of overlap, posing significant difficulties for machine vision-based blended tobacco shred multi-object detection and unbroken tobacco shred rate calculation tasks. This study focuses on the two challenges of identifying blended tobacco shreds and calculating the unbroken tobacco shred rate. In this paper, a new multi-object detection model is developed for blended tobacco shred images based on an improved YOLOv7-tiny model. YOLOv7-tiny is used as the multi-object detection network's mainframe. A lightweight Resnet19 is used as the model backbone. The original SPPCSPC and coupled detection head are replaced with a new spatial pyramid SPPFCSPC and a decoupled joint detection head, respectively. An algorithm for two-dimensional size calculation of blended tobacco shreds (LWC) is also proposed, which is applied to blended tobacco shred object detection images to obtain independent tobacco shred objects and calculate the unbroken tobacco shred rate. The experimental results showed that the final detection precision, mAP@.5, mAP@.5:.95, and testing time were 0.883, 0.932, 0.795, and 4.12 ms, respectively. The average length and width detection accuracy of the blended tobacco shred samples were -1.7% and 13.2%, respectively. The model achieved high multi-object detection accuracy and 2D size calculation accuracy, which also conformed to the manual inspection process in the field. This study provides a new efficient implementation method for multi-object detection and size calculation of blended tobacco shreds in cigarette quality inspection lines and a new approach for other similar blended image multi-object detection tasks.
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Introduction: The classification of the four tobacco shred varieties, tobacco silk, cut stem, expanded tobacco silk, and reconstituted tobacco shred, and the subsequent determination of tobacco shred components, are the primary tasks involved in calculating the tobacco shred blending ratio. The identification accuracy and subsequent component area calculation error directly affect the composition determination and quality of the tobacco shred. However, tiny tobacco shreds have complex physical and morphological characteristics; in particular, there is substantial similarity between the expanded tobacco silk and tobacco silk varieties, and this complicates their classification. There must be a certain amount of overlap and stacking in the distribution of tobacco shreds on the actual tobacco quality inspection line. There are 24 types of overlap alone, not to mention the stacking phenomenon. Self-winding does not make it easier to distinguish such varieties from the overlapped types, posing significant difficulties for machine vision-based tobacco shred classification and component area calculation tasks. Methods: This study focuses on two significant challenges associated with identifying various types of overlapping tobacco shreds and acquiring overlapping regions to calculate overlapping areas. It develops a new segmentation model for tobacco shred images based on an improved Mask region-based convolutional neural network (RCNN). Mask RCNN is used as the segmentation network's mainframe. Convolutional network and feature pyramid network (FPN) in the backbone are replaced with Densenet121 and U-FPN, respectively. The size and aspect ratios of anchors parameters in region proposal network (RPN) are optimized. An algorithm for the area calculation of the overlapped tobacco shred region (COT) is also proposed, which is applied to overlapped tobacco shred mask images to obtain overlapped regions and calculate the overlapped area. Results: The experimental results showed that the final segmentation accuracy and recall rates are 89.1% and 73.2%, respectively. The average area detection rate of 24 overlapped tobacco shred samples increases from 81.2% to 90%, achieving high segmentation accuracy and overlapped area calculation accuracy. Discussion: This study provides a new implementation method for the type identification and component area calculation of overlapped tobacco shreds and a new approach for other similar overlapped image segmentation tasks.
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The primary task in calculating the tobacco shred blending ratio is identifying the four tobacco shred types: expanded tobacco silk, cut stem, tobacco silk, and reconstituted tobacco shred. The classification precision directly affects the subsequent determination of tobacco shred components. However, the tobacco shred types, especially expanded tobacco silk and tobacco silk, have no apparent differences in macro-scale characteristics. The tobacco shreds have small size and irregular shape characteristics, creating significant challenges in their recognition and classification based on machine vision. This study provides a complete set of solutions aimed at this problem for screening tobacco shred samples, taking images, image preprocessing, establishing datasets, and identifying types. A block threshold binarization method is used for image preprocessing. Parameter setting and method performance are researched to obtain the maximum number of complete samples with acceptable execution time. ResNet50 is used as the primary classification and recognition network structure. By increasing the multi-scale structure and optimizing the number of blocks and loss function, a new tobacco shred image classification method is proposed based on the MS-X-ResNet (Multi-Scale-X-ResNet) network. Specifically, the MS-ResNet network is obtained by fusing the multi-scale Stage 3 low-dimensional and Stage 4 high-dimensional features to reduce the overfitting risk. The number of blocks in Stages 1-4 are adjusted from the original 3:4:6:3 to 3:4:N:3 (A-ResNet) and 3:3:N:3 (B-ResNet) to obtain the X-ResNet network, which improves the model's classification performance with lower complexity. The focal loss function is selected to reduce the impact of identification difficulty for different sample types on the network and improve its performance. The experimental results show that the final classification accuracy of the network on a tobacco shred dataset is 96.56%. The image recognition of a single tobacco shred requires 103 ms, achieving high classification accuracy and efficiency. The image preprocessing and deep learning algorithms for tobacco shred classification and identification proposed in this study provide a new implementation approach for the actual production and quality detection of tobacco and a new way for online real-time type identification of other agricultural products.
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In this study, an application of a voltammetric electronic tongue for discrimination and prediction of different varieties of rice was investigated. Different pretreatment methods were selected, which were subsequently used for the discrimination of different varieties of rice and prediction of unknown rice samples. To this aim, a voltammetric array of sensors based on metallic electrodes was used as the sensing part. The different samples were analyzed by cyclic voltammetry with two sample-pretreatment methods. Discriminant Factorial Analysis was used to visualize the different categories of rice samples; however, radial basis function (RBF) artificial neural network with leave-one-out cross-validation method was employed for prediction modeling. The collected signal data were first compressed employing fast Fourier transform (FFT) and then significant features were extracted from the voltammetric signals. The experimental results indicated that the sample solutions obtained by the non-crushed pretreatment method could efficiently meet the effect of discrimination and recognition. The satisfactory prediction results of voltammetric electronic tongue based on RBF artificial neural network were obtained with less than five-fold dilution of the sample solution. The main objective of this study was to develop primary research on the application of an electronic tongue system for the discrimination and prediction of solid foods and provide an objective assessment tool for the food industry.
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Técnicas Electroquímicas/instrumentación , Electrónica/instrumentación , Oryza/química , Análisis Discriminante , Electricidad , Electrodos , Análisis Factorial , Análisis de Fourier , Redes Neurales de la Computación , Soluciones , Factores de Tiempo , LenguaRESUMEN
Peanut meal is the byproduct of high-temperature peanut oil extraction; it is mainly composed of proteins, which have complex tastes after enzymatic hydrolysis to free amino acids and small peptides. The enzymatic hydrolysis method was adopted by using two compound proteases of trypsin and flavorzyme to hydrolyze peanut meal aiming to provide a flavor base. Hence, it is necessary to assess the taste attributes and assign definite taste scores of peanut meal double enzymatic hydrolysis hydrolysates (DEH). Conventionally, sensory analysis is used to assess taste intensity in DEH. However, it has disadvantages because it is expensive and laborious. Hence, in this study, both taste attributes and taste scores of peanut meal DEH were evaluated using an electronic tongue. In this regard, the response characteristics of the electronic tongue to the DEH samples and standard five taste samples were researched to qualitatively assess the taste attributes using PCA and DFA. PLS and RBF neural network (RBFNN) quantitative prediction models were employed to compare predictive abilities and to correlate results obtained from the electronic tongue and sensory analysis, respectively. The results showed that all prediction models had good correlations between the predicted scores from electronic tongue and those obtained from sensory analysis. The PLS and RBFNN prediction models constructed using the voltage response values from the sensors exhibited higher correlation and prediction ability than that of principal components. As compared with the taste performance by PLS model, that of RBFNN models was better. This study exhibits potential advantages and a concise objective taste assessment tool using the electronic tongue in the assessment of DEH taste attributes in the food industry.