RESUMEN
The aim of this research was to examine the effect of the hydroalcoholic extracts from the peel (APE) and pulp (APP) of a traditional apple cultivar from central Italy (Mela Rosa dei Monti Sibillini) on CCl4-induced hepatotoxicity in rats. Phytoconstituents were determined by liquid chromatography-mass spectrometry (LC-MS) analysis showing an abundance of proanthocyanidins and flavonol derivatives together with the presence of annurcoic acid in APE. Wistar rats received APE/APP (30 mg/kg oral administration) for three days before CCl4 injection (2 mL/kg intraperitoneal once on the third day). Treatment with both APE and APP prior to CCl4 injection significantly decreased the serum levels of aspartate aminotransferase (AST), alkaline phosphatase (ALP) and alanine aminotransferase (ALT) compared to the CCl4 group. Besides, pretreatment with APE reversed the CCl4 effects on superoxide dismutase (SOD), myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α) and interleukin-1beta (IL-1ß) levels in liver tissue in rats and reduced tissue damage as shown in hematoxylin and eosin staining. These results showed that this ancient Italian apple is worthy of use in nutraceuticals and dietary supplements to prevent and/or protect against liver disorders.
Asunto(s)
Tetracloruro de Carbono/efectos adversos , Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Enfermedad Hepática Inducida por Sustancias y Drogas/patología , Malus/química , Extractos Vegetales/farmacología , Sustancias Protectoras/farmacología , Animales , Antiinflamatorios , Biomarcadores , Biopsia , Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Modelos Animales de Enfermedad , Inmunohistoquímica , Hígado/efectos de los fármacos , Hígado/metabolismo , Hígado/patología , Estructura Molecular , Extractos Vegetales/química , Sustancias Protectoras/química , RatasRESUMEN
Correction for 'Protective effects of hydroalcoholic extracts from an ancient apple variety 'Mela Rosa dei Monti Sibillini' against renal ischemia/reperfusion injury in rats' by Hasan Yousefi-Manesh et al., Food Funct., 2019, 10, 7544-7552.
RESUMEN
The purpose of this work was to investigate the effect of hydroalcoholic extracts from the peel (APE) and pulp (APP) of a traditional apple variety of central Italy, the 'Mela Rosa dei Monti Sibillini', on the damage caused by renal ischemia/reperfusion injury (IRI) in rats. Thirty mg per kg b.w. of the extracts were administered intraperitoneally to male adult Wistar rats 3 days before the induction of IRI by pedicle clamping. A significant decrease in the levels of malondialdehyde (MDA), tumor necrosis factor-α (TNFα), interleukin 1 beta (IL-1ß) and nuclear factor-κB (NF-κB) was observed in the groups pre-treated with APE when compared with IRI rats. The chemical composition of APE was determined by HPLC-DAD-MSn highlighting a significant amount of proanthocyanidins (52.9 mg g-1), flavonols (42.27 mg g-1) and dihydrochalcones (11.75 mg g-1). These findings indicated that this ancient apple variety is a promising source of nutraceuticals and functional foods helpful to manage complications of renal disorders.
Asunto(s)
Frutas/química , Enfermedades Renales/tratamiento farmacológico , Enfermedades Renales/etiología , Malus/química , Extractos Vegetales/farmacología , Daño por Reperfusión/tratamiento farmacológico , Animales , Antiinflamatorios/química , Antiinflamatorios/farmacología , Regulación hacia Abajo , Inflamación/tratamiento farmacológico , Masculino , FN-kappa B/genética , FN-kappa B/metabolismo , Extractos Vegetales/química , Ratas , Ratas WistarRESUMEN
In the present work, we reported for the first time a comprehensive study on the phytonutrients, that is, natural compounds able to provide benefits to health, found in an ancient apple variety cultivated in orchards of the Sibillini Mountains, central Italy, known as Mela Rosa dei Monti Sibillini. This fruit has recently been promoted by authorities and local institutions as a typical food of the Marche Region. For the purpose, analysis of its nutrients, phenolics, triterpenes and volatile components as well as a morpho-anatomical study was carried out in order to give an added value for its consumption and promotion at regional and national level. ICP-MS, HPLC-MSn and GC-MS analyses were useful techniques for giving a typical fingerprint to this apple, consisting in a high content of K and B, quercetin derivatives as the main phenolic compounds and carboxylic esters, aldehydes, alcohols and (E,E)-α-farnesene as the main key odorants.
