RESUMEN
In synthesis of silver nanoparticles (AgNPs), the composition of the stabilizer used can be closely related to the effectiveness of the synthesis and to the shape of the final nanoparticles. Recently, the use of collagen as an effective nanoparticle stabilization agent was reported. In this work, synthesis of silver nanoparticles using mixed capping agents is reported. The capping agents used were cashew gum-hydrolyzed collagen; kappa carrageenan-hydrolyzed collagen, and agar-hydrolyzed collagen. We evaluated antibacterial action against Gram-positive and Gram-negative bacteria, as well as antifungal activity and cytotoxicity. Homogenized mixtures of collagen and aqueous cashew gum, carrageenan or agar respectively were used to produce the nanoparticles AgNPcolCashew, AgNPcolCarr and AgNPcolAgar. AgNP characterization was performed using Uv-vis, XRD, TEM and DLS and the biological activities were assayed using MIC and MBC analyses for both antibacterial and antifungal application. Results showed that the AgNPcollcar sample showed the strongest bacterial inhibition with MIC values of 62.5 and 31.25⯵M/mL Ag against E. coli and P. aeruginosa respectively. Interestingly, AgNPcollAgar also presented the lowest cytotoxicity when compared with other AgNPs and AgNO3.
Asunto(s)
Colágeno/química , Nanopartículas del Metal/química , Polímeros/química , Plata/química , Plata/farmacología , Animales , Antibacterianos/síntesis química , Antibacterianos/química , Antibacterianos/farmacología , Antibacterianos/toxicidad , Antifúngicos/síntesis química , Antifúngicos/química , Antifúngicos/farmacología , Antifúngicos/toxicidad , Candida albicans/efectos de los fármacos , Técnicas de Química Sintética , Escherichia coli/efectos de los fármacos , Hemólisis/efectos de los fármacos , Hidrólisis , Macrófagos Peritoneales/efectos de los fármacos , Ratones , Pruebas de Sensibilidad Microbiana , Nanotecnología , Pseudomonas aeruginosa/efectos de los fármacos , Ovinos , Plata/toxicidadAsunto(s)
Anacardium/química , Antiinfecciosos/química , Antineoplásicos/química , Cobre/química , Nanopartículas del Metal/química , Gomas de Plantas/química , Animales , Neoplasias de la Mama/tratamiento farmacológico , Línea Celular Tumoral , Supervivencia Celular , Sulfato de Cobre/química , Sulfato de Cobre/toxicidad , Eritrocitos/citología , Macrófagos/citología , Nanopartículas del Metal/toxicidad , Ratones , Ratones Endogámicos BALB C , Ovinos , Staphylococcus aureus/efectos de los fármacos , Propiedades de Superficie , Factores de TiempoRESUMEN
Nanoprecipitation and dialysis methods were employed to obtain nanoparticles (NPs) of acetylated cashew gum (ACG). NPs synthesized by dialysis showed greater average size compared to those synthesized by nanoprecipitation, but they presented improved stability and yield. NPs were loaded with diclofenac diethylamine and the efficiency of the drug incorporation was over 60% for both methods, for an ACG:NP a weight ratio of 10:1. The cytotoxicity assay demonstrated that the NPs had no significant effect on the cell viability, verifying their biocompatibility. The release profile for the diclofenac diethylamine associated with the ACG-NPs showed a more controlled release compared to the free drug and a Fickian diffusion mechanism was observed. Transdermal permeation reached 90% penetration of the drug.