Biochemical and Histological Biomarkers in Crassostrea sp. (Bivalvia, Ostreidae) for Environmental Monitoring of a Neotropical Estuarine Area (São José Bay, Northeastern Brazil).

This study aimed to compare biochemical and histological biomarkers in oysters to identify impacted areas in a Brazilian port region. Oysters belonging to the Crassostrea genus were collected in two points in São José Bay (Brazil): (A1) Curupu Island (control area) and (A2) Braga Port (impacted area). Digestive glands from oysters were used to analyze the enzymatic activity of glutathione S-transferase and Catalase. The gills were used for standard histology analyses. Water samples were collected for metal analyses. Our results indicated that there was a change in the activity of oyster GST and CAT enzymes, especially in A2. Histological gill analysis indicated more frequent changes in A2. The analyzed metals presented higher values in A2. The results of this study suggest that enzymatic alterations, histological changes and higher metal values are indicative of initial stress caused by contaminants in São José Bay, especially in the port region.

Biomarkers (glutathione S-transferase and catalase) and microorganisms in soft tissues of Crassostrea rhizophorae to assess contamination of seafood in Brazil.

The goal of this study was to evaluate biomarkers (glutathione S-transferase and catalase) and microorganisms in soft tissues of Crassostrea rhizophorae to assess possible contamination of seafood in Brazil. The oysters were sampled from a reference area (Ports 1 and 2) and an impacted area (Ports 3 and 4) in Brazil (São Luís Island, Maranhão). Six attributes were examined in sampled oysters: glutathione S-transferase activity, catalase activity, concentrations of total coliforms and thermotolerant coliforms, and levels of Escherichia coli and Aeromonas hydrophila. Water samples were analysed for aluminium, cadmium, iron, manganese, lead, mercury, phenolics, and polychlorinated biphenyls. We found that Ports 3 and 4 are impacted by several contaminants (mercury, phenolics, and polychlorinated biphenyls), while Ports 1 and 2 are still relatively free of these contaminants. Changes in enzymes activity as well as the highest tissue bacterial concentrations were recorded in oysters from Ports 3 and 4 during the rainy season.

Enzymatic and Histological Biomarkers in Ucides cordatus (Crustacea, Decapoda) in an Industrial Port on the North Coast of Brazil.

The aim of this study was to evaluate enzymatic (glutathione-S-transferase and catalase) and histological (branchial lesions) biomarkers in Ucides cordatus (Crustacea, Decapoda) from an industrial port region on the north coast of Brazil. The crabs were collected in two distinct locations of the Brazilian coast: A1 = region under influence of port activities; and A2 = low-impacted area. We performed histological examination in the gills and glutathione-S-transferase (GST) and catalase activity in the hepatopancreas. The most frequent and severe histological lesions were found in A1, especially rupture of pilaster cells and lamellar collapse. Catalase activity did not show a pattern capable of differentiating the two analyzed areas. On the other hand, GST activity presented a more pronounced response in the crabs of the port area (p < 0.05), coinciding with the most frequent branchial lesions in these same organisms. These results suggest that the species is susceptible to environmental stress, once alterations at different organizational levels were verified.