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1.
Environ Monit Assess ; 194(10): 673, 2022 Aug 16.
Artículo en Inglés | MEDLINE | ID: mdl-35972702

RESUMEN

Groundwater stands as a unique source of water supply in Kabul city, Afghanistan. In this investigation, 35 samples of groundwater were comprehensively analyzed to determine its hydrogeochemical characterizations, quality, water types, and its acceptability as drinking sources. A portable digital multiparameter instrument (LAB MAN Scientific instrument) was used to measure the total dissolved solids (TDS), hydrogen potential (pH), and electrical conductivity (EC). Total hardness, chloride, and bicarbonate were examined via a titrimetric approach. Sodium, calcium, magnesium, and potassium concentrations were measured with a flame photometer. Fluoride was determined by using a digital portable multiparameter. UV-VIS spectrophotometers were employed to count sulfate and nitrate concentrations. The distribution pattern of measured parameters and the Water Quality Index (WQI) in groundwater were spatially modeled utilizing the ArcGIS tool. The findings provide insight into the main anions and cations, which are found in ascending sequence F < NO3 < SO4 < Cl < HCO3 and K < Ca < Na < Mg, respectively. Based on the measurements of ion concentrations, bicarbonate (71.4%), chloride (14.28%), nitrate (2.85%), magnesium (80%), sodium (82.85%), calcium (5.71%), and potassium (17.14%) were all determined to be over the World Health Organization (WHO) limits of drinking water. Using the Piper trilinear diagram, two significant hydrochemical facies (CaNaHCO3 and NaHCO3) were discovered. Based on the mathematical model of WQI outputs, 88.57% of the research region has excellent to good water, whereas 11.43% has poor to very poor water.


Asunto(s)
Agua Subterránea , Contaminantes Químicos del Agua , Afganistán , Bicarbonatos , Calcio , Cloruros/análisis , Monitoreo del Ambiente , Sistemas de Información Geográfica , Agua Subterránea/química , Humanos , Magnesio/análisis , Nitratos/análisis , Potasio , Sodio , Contaminantes Químicos del Agua/análisis , Calidad del Agua
2.
Biochimie ; 180: 23-29, 2021 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-33132160

RESUMEN

Split luciferase complementary assay has been used to investigate the effect of WD domain deletion on Apaf-1 oligomerization. Apaf-1 is an adaptor molecule in formation of apoptosome that activates caspase-9, an activation that is a key event in the mitochondrial cell death pathway. Structural studies suggest that normally Apaf-1 is held in an inactive conformation by intramolecular interactions between Apaf-1's nucleotide binding domain and one of its WD40 domains (WD1). In the prevailing model of Apaf-1 activation, cytochrome c binds to sites in WD1 and in Apaf-1's second WD40 domain (WD2), moving WD1 and WD2 closer together and rotating WD1 away from the nucleotide binding domain. This allows Apaf-1 to bind dATP or ATP and to form the apoptosome, which activates caspase-9. This model predicts that cytochrome c binding to both WD domains is necessary for apoptosome formation and that an Apaf-1 with only WD1 will be locked in an inactive conformation that cannot be activated by cytochrome c. Here we investigated the effect of removing one WD domain (Apaf-1 1-921) on Apaf-1 interactions and caspase activation. Apaf-1 1-921 could not activate caspase-9, even in the presence of cytochrome c. These data show that a single WD domain is sufficient to lock Apaf-1 in an inactive state and this state cannot be altered by cytochrome c.


Asunto(s)
Apoptosomas/química , Apoptosomas/metabolismo , Factor Apoptótico 1 Activador de Proteasas/química , Factor Apoptótico 1 Activador de Proteasas/metabolismo , Repeticiones WD40/fisiología , Factor Apoptótico 1 Activador de Proteasas/genética , Caspasa 3/metabolismo , Caspasa 9/metabolismo , Citocromos c/metabolismo , Nucleótidos de Desoxiadenina/metabolismo , Activación Enzimática , Células HEK293 , Humanos , Luciferasas/metabolismo , Mediciones Luminiscentes/métodos , Mutación/genética , Unión Proteica , Estructura Cuaternaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo
3.
Arch Biochem Biophys ; 693: 108574, 2020 10 30.
Artículo en Inglés | MEDLINE | ID: mdl-32898566

RESUMEN

Studies reported that Δ9-tetrahydrocannabinol (Δ9-THC) is an essential drug as an anti-cancer, neuroprotective, anti-inflammatory, and immune-modulatory agent. However, the mechanism by which Δ9-THC causes these events remains to be elucidated. We attempted to investigate the in vivo studies of Δ9-THC on brain microtubule dynamicity, and acetylcholinesterase (AChE) activity. The microtubule polymerization, secondary and tertiary structures of α/ß-tubulins, as well as the AChE activity, were evaluated in the experimental groups. The significantly lowest optical density and initial rate of polymerization was observed in THC 3 mg/kg, THC 9 mg/kg, and THC 18 mg/kg treated groups. The content of secondary and tertiary structures of α/ß-tubulins was significantly affected in treated groups. The AChE activity was significantly lower in treated groups in a dose-dependent manner. These data highlight the microtubule dynamicity as a molecular target for Δ9-THC, which affects memory dysfunction. However, Δ9-THC can be inhibited the AChE activity and provide an improved therapeutics for neurodegenerative diseases.


Asunto(s)
Dronabinol/farmacología , Microtúbulos/efectos de los fármacos , Acetilcolinesterasa/efectos de los fármacos , Animales , Inhibidores de la Colinesterasa/farmacología , Dicroismo Circular , Relación Dosis-Respuesta a Droga , Polimerizacion , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , Ratas , Tubulina (Proteína)/química , Tubulina (Proteína)/efectos de los fármacos , Tubulina (Proteína)/aislamiento & purificación
4.
Arch Biochem Biophys ; 650: 39-48, 2018 07 15.
Artículo en Inglés | MEDLINE | ID: mdl-29758202

RESUMEN

Ursolic Acid (UA), a pentacyclic triterpenoid compound, plays a vital role in aging process. However, the role of UA in the regulation of aging and longevity is still controversial as we have previously demonstrated that UA increases SIRT1 protein level in aged-mice. Here, we reveal that UA directly activates SIRT1 in silico, in vitro and in vivo. We have identified that UA binds to outer surface of SIRT1 and leads to tight binding of substrates to enzyme in comparison with Resveratrol (RSV) and control. Furthermore, our results indicate that UA drives the structure of SIRT1 toward a closed state (an active form of enzyme). Interestingly, our experimental findings are in agreement with the molecular dynamic results. Based on our data, UA increases the affinity of enzyme for both substrates with decreasing Km value, while enhances the Vmax of enzyme. Additionally, we have determined that UA heightened SIRT1 catalytic efficiency by 2 folds compared with RSV. Thereby, to identify the endogenous activator of SIRT1, UA was administrated to aged-mice and then the tissues were isolated. According to our results, it can be concluded that UA increases SIRT1 activity and mimics Lamin A and AROS behavior in the living cells.


Asunto(s)
Activadores de Enzimas/farmacología , Sirtuina 1/metabolismo , Triterpenos/farmacología , Sitio Alostérico/efectos de los fármacos , Animales , Activación Enzimática/efectos de los fármacos , Humanos , Lamina Tipo A/metabolismo , Masculino , Ratones Endogámicos C57BL , Simulación del Acoplamiento Molecular , Conformación Proteica/efectos de los fármacos , Sirtuina 1/química , Ácido Ursólico
5.
Arch Biochem Biophys ; 642: 46-51, 2018 03 15.
Artículo en Inglés | MEDLINE | ID: mdl-29410086

RESUMEN

Apaf-1 is a cytosolic multi-domain protein in the apoptosis regulatory network. When cytochrome c releases from mitochondria; it binds to WD-40 repeats of Apaf-1 molecule and induces oligomerization of Apaf-1. Here in, a split luciferase assay was used to compare apoptosome formation in cell-free and cell-based systems. This assay uses Apaf-1 tagged with either N-terminal fragment or C-terminal fragment of P. pyralis luciferase. In cell based-system, the apoptosome formation is induced inside the cells which express Apaf-1 tagged with complementary fragments of luciferase while in cell-free system, the apoptosome formation is induced in extracts of the cells. In cell-free system, cytochrome c dependent luciferase activity was observed with full length Apaf-1. However, luciferase activity due to apoptosome formation was much higher in cell based system compared to cell-free system. The truncated Apaf-1 which lacks WD-40 repeats (ΔApaf-1) interacted with endogenous Apaf-1 in a different fashion compared to native form as confirmed by different retention time of eluate in gel filtration and binding to affinity column. The interactions between endogenous Apaf-1 and ΔApaf-1 is stronger than its interaction with native exogenous Apaf-1 as indicated by dominant negative effect of ΔApaf-1 on caspase-3 processing.


Asunto(s)
Apoptosomas/metabolismo , Factor Apoptótico 1 Activador de Proteasas/metabolismo , Apoptosis , Factor Apoptótico 1 Activador de Proteasas/química , Biopolímeros/metabolismo , Caspasa 3/metabolismo , Caspasa 9/metabolismo , Sistema Libre de Células , Cromatografía de Afinidad , Cromatografía en Gel , Activación Enzimática , Células HEK293 , Humanos , Luciferasas/metabolismo , Unión Proteica , Proteolisis , Repeticiones WD40
6.
Appl Biochem Biotechnol ; 172(6): 3116-27, 2014 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-24492953

RESUMEN

Ionic liquids as neoteric solvents, microwave irradiation, and alternative energy source are becoming as a solvent for many enzymatic reactions. We recently showed that the incubation of firefly luciferase from Photinus pyralis with various ionic liquids increased the activity and stability of luciferase. Magnetic nanoparticles supported ionic liquids have been obtained by covalent bonding of ionic liquids-silane on magnetic silica nanoparticles. In the present study, the effects of [γ-Fe2O3@SiO2][BMImCl] and [γ-Fe2O3@SiO2][BMImI] were investigated on the structural properties and function of luciferase using circular dichroism, fluorescence spectroscopy, and bioluminescence assay. Enzyme activity and structural stability increased in the presence of magnetic nanoparticles supported ionic liquids. Furthermore, the effect of ingredients which were used was not considerable on K(m) value of luciferase for adenosine-5'-triphosphate and also K(m) value for luciferin.


Asunto(s)
Enzimas Inmovilizadas/química , Luciérnagas/química , Líquidos Iónicos/química , Luciferasas de Luciérnaga/química , Nanopartículas de Magnetita/química , Adenosina Trifosfato/química , Animales , Estabilidad de Enzimas , Compuestos Férricos/química , Luciérnagas/enzimología , Cinética , Nanopartículas de Magnetita/ultraestructura , Silanos/química
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