RESUMEN
The decomposition of organic matter mediated by soil enzymes is the key process that transports carbon from the soil into the atmosphere. To better understand the effect of global warming on organic matter decomposition, we evaluated the temperature sensitivity (Q10) of invertase (EC3.2.1.26), ß-glucosidase (EC3.2.1.21), urease (EC3.1.1.5), acid phosphatase (EC3.1.3.2), and arylsulfatase (EC3.1.6.1) activities in red soil from the subtropical region and black soil from the mid-temperate region at 5, 15, 25, 35, and 45 °C. Further, the in-situ stoichiometry of the products released by enzymes was modelled. All of the enzyme activities in the tested soils increased with the increasing temperature (1.1-8.9 fold per 10 °C), indicating an enhanced degradation of the organic substrate with warming. In the lower temperature range (5-25 °C), Q10 of the enzyme activities in the red soil evaluated in terms of total enzyme activity index were more prominent than that in black soil (1.53 and 3.46 vs 1.16 and 3.19). Changes in the in-situ stoichiometry of enzyme products with warming indicated that, in colder months (Jan. to Apr. and Oct. to Dec.), the microbial nutrient demand in the red soil exhibited the following order, N > P > S > C. While in the black soil, it suggested that there is increasing microbial demand for only N and S. In the warmer months (May to Sep.), the microbial nutrient demands in the two soils were opposite to the colder months. The results suggested differential changes in microbial nutrient limitation with warming, which has significant implications for the carbon stocks management in farmlands under the changing global climate.
Asunto(s)
Microbiología del Suelo , Suelo , Carbono , Granjas , Calentamiento Global , NutrientesRESUMEN
Arsenate [As(V)], in general, is associated with various aggregates and exists as different species in soil, which in turn influences its toxicity and potential contamination. Previous studies have demonstrated the usefulness of alkaline phosphatases (ALP) to evaluate As(V) pollution. However, the effect of different arsenic fractions on ALP among soil aggregates is still unclear. Thus, the distribution of As fractions and ALP kinetics was determined in four-month As-aged paddy soil aggregates. Results revealed the two major fractions of As in aggregates were humic-bound and Fe and Mn oxides-bound [both around 30% under 800 mg kg-1 of As(V)]. Besides, it was observed that available soil phosphorus could positively affect the relative content of water-soluble, exchangeable and carbonate-bound arsenic. In the kinetics experiment, both the Michaelis-Menten constant (Km) and maximum reaction velocity (Vmax) of ALP increased with increasing As(V) concentration under four months ageing for each size aggregate. Multiple linear stepwise regression analysis between kcat and the relative content of arsenic fraction indicated that carbonate-bound arsenic is the main fraction that inhibited the kcat for macroaggregates (> 0.25 mm size). For soil aggregates of 0.1-0.25 mm size, kcat increased with an increase in arsenic residual fraction. As for aggregates <0.1 mm size, Fe and Mn oxide-bound fraction is the main fraction that inhibited the kcat. Overall, this study suggests carbonate-bound and Fe and Mn oxide-bound arsenic fractions could decrease the ALP activities via a decrease in the catalytic efficiency in macroaggregates and <0.1 mm size aggregates, respectively. Besides, available phosphorus should be considered as the main factor when assessing As biotoxicity and mobility.