Effects of partial maize silage substitution with microalgae on viscosity and biogas yields in continuous AD trials.

The microalga Acutodesmus obliquus was investigated as a feedstock in semi-continuously fed anaerobic digestion trials, where A. obliquus was co-digested with pig slurry and maize silage. Maize silage was substituted by both 10% and 20% untreated, and 20% ultrasonicated microalgae biomass on a VS (volatile solids) basis. The substitution of maize silage with 20% of either ultrasonicated and untreated microalgae led to significantly lower biogas yields, i.e., 560 dm³ kg-1 VScorr in the reference compared to 516 and 509 dm³ kg-1VScorr for untreated and ultrasonicated microalgae substitution. Further, the viscosities in the different reactors were measured at an OLR of 3.5 g VS dm-3 d-1. However, all treatments with microalgae resulted in significantly lower viscosities. While the mean viscosity reached 0.503 Pa s in the reference reactor, mean viscosities were 53% lower in reactors where maize was substituted by 20% microalgae, i.e. 0.239 Pa s, at a constant rotation speed of 30 rpm. Reactors where maize was substituted by 20% ultrasonicated microalgae had a 32% lower viscosity, for 10% microalgae substitution a decrease of 8% was measured. Decreased viscosities have beneficial effect on the bioprocess and the economy in biogas plants. Nonetheless, with regard to other parameters, no positive effect on biogas yields by partial substitution with microalgae biomass was found. The application of microalgae may be an interesting option in anaerobic digestion when fibrous or lignocellulosic substances lead to high viscosities of the digested slurries. High production costs remain the bottleneck for making microalgae an interesting feedstock.

Lower Body vs. Upper Body Resistance Training and Arterial Stiffness in Young Men.

Resistance training has been shown to increase arterial stiffness. The purpose of the present study was to examine and compare the systemic arterial stiffness responses to acute lower body (LRT) and upper body (URT) resistance training. 20 healthy young men [median age: 26 years (interquartile range 23, 32)] underwent LRT, URT and whole body resistance training (WRT). Before and immediately after, as well as 20, 40 and 60 min after each training session, we measured the cardio-ankle vascular index (CAVI) and brachial-ankle pulse wave velocity (baPWV) using VaSera VS-1500 N. We used mixed models for repeated measurements to estimate the post-exercise differences in CAVI and baPWV between the 3 resistance training modes. Immediately after exercise cessation, both CAVI and baPWV were lower for LRT compared with URT [CAVI: - 0.93 (95% confidence interval [CI] - 1.15, - 0.70); baPWV: - 2.08 m/s (95% CI - 2.48, - 1.67)]. Differences between LRT and URT gradually decreased during follow-up. Compared with WRT, LRT induced a decrease and URT an increase in arterial stiffness across all time points. In conclusion, LRT presents more favorable post-exercise arterial stiffness than URT. Our results suggest that LRT or WRT may be preferred over URT in individuals with impaired arterial stiffness.

Effects of acute bouts of endurance exercise on retinal vessel diameters are age and intensity dependent.

Alterations of retinal vessel diameters are associated with increased cardiovascular risk. We aimed to investigate changes in retinal vessel diameters in response to acute dynamic exercise of different intensities and whether these changes are age dependent. Seventeen healthy seniors (median (IQR) age 68 (65, 69) years) and 15 healthy young adults (median (IQR) age 26 (25, 28) years) first performed a maximal treadmill test (MTT) followed by a submaximal treadmill test (SMTT) and a resting control condition in randomised order. Central retinal arteriolar (CRAE) and central retinal venular (CRVE) diameter equivalents were measured before as well as 5 (t5) and 40 (t40) minutes after exercise cessation using a static retinal vessel analyser. Both exercise intensities induced a significant dilatation in CRAE and CRVE at t5 compared to the control condition (P < 0.001). At t40, the mean increase in CRAE and CRVE was greater for MTT compared to that for SMTT (CRAE 1.7 µm (95 % confidence interval (CI) -0.1, 3.6; P = 0.061); CRVE 2.2 µm (95 % CI 0.4, 4.1; P = 0.019)). However, the estimated difference at t5 between seniors and young adults in their response to MTT compared to SMTT was 5.3 µm (95 % CI 2.0, 8.5; P = 0.002) for CRAE and 4.1 µm (95 % CI -0.4, 8.6; P = 0.076) for CRVE. Wider arteries and veins after maximal versus submaximal exercise for seniors compared to young adults suggest that myogenic vasoconstriction in response to exhaustive exercise may be reduced in seniors. Age-related loss of vascular reactivity has clinical implications since the arteriolar vasoconstriction protects the retinal capillary bed from intraluminal pressure peaks.

Medieval cattle from Bern (Switzerland): an archaeozoological, genetic and historical approach.

This study deals with genetic analyses of an assemblage of mediaeval (13th century) cattle metapodials from Bern that had previously been osteometrically examined regarding sex, shape and wither height. The results from the genetic sexing of these small (height 100 to 120 cm) cattle correlate well with the osteometric interpretations. Some few exceptions we interpreted as cows used as draft animals with stouter bones and thus osteometrically determined as males. Two morphologically different groups of cow metatarsals however, we took as proof of the historical fact that Bern relied on livestock from different geographical origins: the town's vicinity and the alpine pastures with their favourable grazing conditions. It was not possible to distinguish them genetically. An analysis of one single nucleotide polymorphism (SNP) in the melanocortin receptor 1 (MC1R) showed that predominant coat colour most likely was red-brown. Furthermore, an analysis of the SNP in the Y-chromosomal intron UTY19 that divide modern taurine cattle in two major haplogroups (Y1 and Y2) showed that the mediaeval cattle belonged to the haplogroup Y2 with one single exception of a Y1.

Is routine biopsy of the lacrimal sac wall indicated at dacryocystorhinostomy? A prospective study and literature review.

OBJECTIVE: To determine whether routine biopsy of the lacrimal sac wall at dacryocystorhinostomy (DCR) is indicated. METHODS: A prospective study and literature review. In 193 consecutive endoscopic DCRs performed on 164 patients (108 females and 56 males) part of the medial wall of the lacrimal sac was sent for histological examination. The mean age of the patients was 64 years with a range of 2.5-89 years. Previous reported series were reviewed. RESULTS: Of the 193 specimens, 44 (23%) showed normal histology, 146 (76%) showed varying degrees of non-specific chronic inflammation, and three (1.2%) showed specific pathology. Of the three specimens that showed specific pathology two showed sarcoidosis and one showed transitional cell papilloma. The two specimens with sarcoidosis were obtained from one patient who underwent bilateral surgery. In this and the six previous reported series only seven of 1294 specimens (0.5%) showed specific pathology, which was definitely not suspected preoperatively or intraoperatively, and only one of these (0.08%) was found to be malignant (a lymphoma). CONCLUSIONS: Biopsy of the lacrimal sac wall at DCR is not indicated routinely and is only indicated if there is a reason to suspect pathology other than chronic inflammation preoperatively or intraoperatively.

[The blood group distribution in domestic cats in Switzerland]. / Die Blutgruppenverteilung bei den Hauskatzen in der Schweiz.

1014 Swiss housecats have been blood typed. 99.6% had blood group A and 0.4% had blood group B, with no type AB cats detected. The transfusion of blood, or the transfer of antibodies to newborn kittens through colostrum, can cause clinical signs of incompatibility. Because almost all B-cats have high naturally occurring antibody titers against the other blood group, it is the B-cat which is most likely at risk to have incompatibility reactions after a blood transfusion and it is the B-queen which has high antibody titers in the colostrum. In the contrary only about one third of the A-cats have antibodies and usually in low titers. The present investigation demonstrates that the incidence of blood type B in Swiss housecats is very low. Therefore, it can be concluded that there is a minimal risk for incompatibility reactions due to blood transfusions. The same could be said of neonatal isoerythrolysis which is unlikely to occur with the random mating of housecats.

[CEA test in domestic animals]. / CEA-test beim Haustier.

In human cancer treatment, CEA (carcino embryonic antigen) testing is a routine procedure, even though the test is of low sensitivity (40%) and low specificity (70%). Since tests with polyclonal antibodies render no reproducible results with animal sera, the applicability of a recently available monoclonal CEA test designed for human sera was evaluated. We were able to show that the latter test was of supplemental diagnostic value when testing animal sera. The upper normal limit for dogs is 1.65 ng/ml, for cats 2.81 ng/ml, for cows 2.85 ng/ml, for sheep 2.85 ng/ml and for horses 1.61 ng/ml.

Relationships of total protein, specific gravity, viscosity, refractive index and latex agglutination to immunoglobulin G concentration in mare colostrum.

A colostrum sample was collected within 24 h after foaling from 27 mares and from 10 other mares a milk sample was collected several weeks post partum. Immunoglobulin G concentrations were determined quantitatively by radial immunodiffusion and semi-quantitatively using a commercial latex agglutination test. Total protein, specific gravity, viscosity and refractive index were determined and their relationships to the immunoglobulin G concentration analysed. All parameters correlated with the immunoglobulin G concentration. The latex agglutination test divided the colostrum samples into three groups with different means for immunoglobulin G and total protein concentrations. Specific gravity and the latex agglutination test were found to be the methods best suited for on-farm evaluation of colostrum quality.