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1.
J Med Food ; 24(5): 533-540, 2021 May.
Artículo en Inglés | MEDLINE | ID: mdl-34009024

RESUMEN

We evaluated the effects of unripe mandarin orange (Citrus unshiu) extract powder (unripe mandarin extract powder [UMEP]) treated with subcritical water on allergic diseases by using animal models. High performance liquid chromatography (HPLC) analysis revealed that subcritical water is a more effective solvent than alcohol and hot water, as it quickly extracted approximately 90% of the functional compounds narirutin (1) and hesperidin (2) from whole fruits. Repeated oral administration of UMEP significantly reversed the peripheral blood flow decline observed during the promotion of allergies after sensitization with the antigen, hen egg-white lysozyme (HEL). UMEP also significantly inhibited compound 48/80-induced scratching behavior in HEL-sensitized mice, which are more sensitive to itching stimuli than are normal mice, without suppressing locomotor activity. In addition, repeated oral administration of UMEP in ovalbumin-challenged guinea pigs significantly suppressed the late phase of nasal airway resistance. This study provides evidence that the subcritical water extract powder of unripe C. unshiu fruit is an effective anti-allergic functional food.


Asunto(s)
Antialérgicos , Citrus , Animales , Antialérgicos/farmacología , Cobayas , Ratones , Extractos Vegetales , Polvos , Agua
2.
J Clin Biochem Nutr ; 67(1): 84-88, 2020 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-32801473

RESUMEN

In this study, we employed a previously developed in vivo assay system to determine whether the flowers and leaves of Syringa vulgaris (S. vulgaris; commonly known as "lilac") can prevent blood stasis syndrome, known as oketsu in Japanese. This syndrome is considered an important pathology in traditional Chinese and Japanese medicine, and is related to diseases such as peripheral vascular disorders, blood vessel inflammation, and platelet aggregation, whose severities are augmented owing to lipid peroxidation, free radicals, and oxidative stress. The assay system employed in this study monitored the blood flow decrease in the tail vein of mice subjected to sensitization with hen egg white lysozyme. Through bioassay-guided fractionation of different S. vulgaris extracts, five polyphenols were isolated and identified. Among them, quercetine 3-glucoside, quercetin 3-rutinoside, and acteoside were identified as active compounds, as they significantly mitigated blood flow reduction. These findings indicate that the polyphenols obtained from S. vulgaris could be useful for preventing oketsu and improve the quality of life of individuals with disorders and diseases such as gynecopathy, cold sensitivity, poor circulation, allergy, and lifestyle-related diseases.

3.
J Nat Med ; 72(2): 582-587, 2018 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-29468577

RESUMEN

Linarinic acid, (-)-1,2,3,9-tetrahydropyrrolo[2,1-b]quinazoline-1-carboxylic acid (4a), was isolated from the ethanol extract of Linaria vulgaris Mill. In our previous study, a series of tetrahydropyrrolo[2,1-b]quinazoline derivatives 4b, 4c, 5a, 5b, 6a and 6b that were structurally related to 4a and evaluated as neuroprotective agents were synthesized. The aim of the present study was to investigate the novel features of these compounds. We examined their allergy-preventive effects using an in vivo assay system we developed previously, that monitors a decrease in blood flow in the tail vein of mice subjected to sensitization with hen egg-white lysozyme. We observed that 4a and its three derivatives, amide (6a), ester (5a), bromine (4b), and alcohol substituent (6b), showed significant allergy-preventive activities. The study confirmed the allergy-preventive activity of tetrahydropyrrolo[2,1-b]quinazoline derivatives by comprehensively monitoring the specific blood flow decrease occurring in the induction phase of allergy. This finding may aid in the development of new agents for the treatment of allergic diseases such as atopic dermatitis, allergic asthma, and hay fever.


Asunto(s)
Hipersensibilidad/prevención & control , Linaria/química , Quinazolinas/química , Animales , Hipersensibilidad/tratamiento farmacológico , Ratones
4.
Biol Pharm Bull ; 39(5): 874-8, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-27150154

RESUMEN

Stress exacerbates allergic disorders such as atopic dermatitis and asthma. It is also an important factor affecting blood flow (BF). Allergic reactions also affect blood flow. For example, we observed that mice sensitized with hen egg-white lysozyme (HEL) have decreased BF during the allergy induction phase. Based on this finding, we established a model for evaluating chronic restraint stress-enhanced allergies. Mice were sensitized with 12.5 µg/head of HEL on day 0, then restrained for 90 min daily on days 1-3, 5, and 6 in a modified 50 mL polystyrene conical centrifuge tube with multiple air holes for ventilation. We used the decrease in BF during that time as a guide for developing an in vivo assay for substances that can inhibit stress-enhanced allergies. Finally, we demonstrated the utility of the new method by testing crude drugs that are used solely or in combination with other crude drugs to treat stress-related illness and neuropsychiatric symptoms. Our model should be useful for identifying potential anti-stress-enhanced allergy drugs.


Asunto(s)
Antialérgicos/farmacología , Bioensayo , Mezclas Complejas/farmacología , Hipersensibilidad/tratamiento farmacológico , Flujo Sanguíneo Regional/efectos de los fármacos , Estrés Psicológico/complicaciones , Animales , Antígenos , Corticosterona/sangre , Hipersensibilidad/complicaciones , Hipersensibilidad/fisiopatología , Magnoliopsida/química , Masculino , Ratones , Muramidasa , Poria/química , Restricción Física , Estrés Psicológico/fisiopatología , Cola (estructura animal)/irrigación sanguínea
5.
Biol Pharm Bull ; 34(8): 1330-3, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-21804227

RESUMEN

Allergy-preventive activity of flower buds of Lonicera japonica THUNB. was found in the 35% EtOH extract (LJ) using an in vivo assay, The assay system uses monitoring of a decrease in blood flow (BF) in the tail vein of mice subjected to sensitization with hen-egg white lysozyme (HEL). Bioassay-guided fractionation of the 35% EtOH extract led to isolation of chlorogenic acid (1) and three known iridoid derivatives, loganin (2), secoxyloganin (3) and sweroside (4), all of which inhibited the BF decrease. This suggested that the flower buds of L. japonica and compounds isolated from them have allergy-preventive properties. The structure-activity relationship of iridoid derivatives, morroniside (5), geniposide (6), asperuloside (7), aucubin (8) and catalpol (9), were also tested using the same bioassay method. Compounds 2-5 and 9 having the sp(3) atom at C-8 showed an allergy-preventive effect, while compounds 6, 7 and 8 having a double bond at C-7, C-8 did not.


Asunto(s)
Ácido Clorogénico/uso terapéutico , Hipersensibilidad/prevención & control , Iridoides/uso terapéutico , Lonicera/química , Fitoterapia , Extractos Vegetales/uso terapéutico , Animales , Antialérgicos/aislamiento & purificación , Antialérgicos/farmacología , Antialérgicos/uso terapéutico , Pollos , Ácido Clorogénico/aislamiento & purificación , Ácido Clorogénico/farmacología , Femenino , Flores/química , Hipersensibilidad/inmunología , Glucósidos Iridoides/aislamiento & purificación , Glucósidos Iridoides/farmacología , Glucósidos Iridoides/uso terapéutico , Iridoides/aislamiento & purificación , Iridoides/farmacología , Masculino , Ratones , Ratones Endogámicos , Muramidasa , Extractos Vegetales/química , Extractos Vegetales/farmacología , Relación Estructura-Actividad , Venas
6.
J Nat Med ; 65(3-4): 610-6, 2011 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-21479861

RESUMEN

Kinka-cha (dried leaf of Camellia nitidissima) is used as a folk tea for detoxication, diuresis and antihypertension. In the present study, we evaluated the extract of kinka-cha on metabolic, vascular and oxidative stress parameters in a model of metabolic syndrome, SHR/NDmcr-cp/cp (SHR/cp) rats that manifest hypertension, obesity, glucose intolerance and hyperlipidemia. Treatment with the extract of kinka-cha alleviated the increase in blood pressure, decrease in tail blood flow and elevated serum oxidative stress marker levels including lipid peroxides, 8-hydroxy-2'-deoxyguanosine, 3-nitrotyrosine and 3-chlorotyrosine. However, kinka-cha did not affect weight gain, hyperglycemia and hyperlipidemia, nor the relaxation responses of the aorta mesenteric artery, thoracic aortas and tail vein, and blood clotting and platelet aggregation. These results suggest that kinka-cha can help reduce the risk of developing metabolic syndrome, possibly due to the presence of antioxidants.


Asunto(s)
Síndrome Metabólico/sangre , Síndrome Metabólico/tratamiento farmacológico , Extractos Vegetales/uso terapéutico , Té/química , Animales , Presión Sanguínea/efectos de los fármacos , Cromatografía Líquida de Alta Presión , Modelos Animales de Enfermedad , Femenino , Extractos Vegetales/química , Ratas , Ratas Endogámicas WKY , Flujo Sanguíneo Regional/efectos de los fármacos
7.
Biosci Biotechnol Biochem ; 75(1): 7-12, 2011.
Artículo en Inglés | MEDLINE | ID: mdl-21228488

RESUMEN

The uptake activity ratio for AMP, ADP, and ATP in mutant (T-1) cells of Escherichia coli W, deficient in de novo purine biosynthesis at a point between IMP and 5-aminoimidazole-4-carboxiamide-1-ß-D-ribofuranoside (AICAR), was 1:0.43:0.19. This ratio was approximately equal to the 5'-nucleotidase activity ratio in E. coli W cells. The order of inhibitory effect on [2-³H]ADP uptake by T-1 cells was adenine > adenosine > AMP > ATP. About 2-fold more radioactive purine bases than purine nucleosides were detected in the cytoplasm after 5 min in an experiment with [8-¹4C]AMP and T-1 cells. Uptake of [2-³H]adenosine in T-1 cells was inhibited by inosine, but not in mutant (Ad-3) cells of E. coli W, which lacked adenosine deaminase and adenylosuccinate lyase. These experiments suggest that AMP, ADP, and ATP are converted mainly to adenine and hypoxanthine via adenosine and inosine before uptake into the cytoplasm by E. coli W cells.


Asunto(s)
Nucleótidos de Adenina/metabolismo , Escherichia coli/metabolismo , 5'-Nucleotidasa/metabolismo , Nucleótidos de Adenina/farmacología , Adenosina Difosfato/metabolismo , Adenosina Difosfato/farmacología , Adenosina Monofosfato/metabolismo , Adenosina Monofosfato/farmacología , Adenosina Trifosfato/metabolismo , Adenosina Trifosfato/farmacología , Transporte Biológico/efectos de los fármacos , Radioisótopos de Carbono , Citoplasma/enzimología , Citoplasma/metabolismo , Escherichia coli/citología , Escherichia coli/genética , Escherichia coli/crecimiento & desarrollo , Mutación , Nucleotidasas/metabolismo , Periplasma/enzimología , Periplasma/metabolismo , Purina-Nucleósido Fosforilasa/metabolismo
8.
Biol Pharm Bull ; 33(4): 714-6, 2010.
Artículo en Inglés | MEDLINE | ID: mdl-20410612

RESUMEN

The allergy-preventive activity of a 35% EtOH extract (IT) of flowers of Impatiens textori MIQ. was demonstrated in a continuing search for allergy-preventive substances from natural sources. The evaluation of its activity used an in vivo assay method for monitoring the blood flow decrease in the tail vein microcirculation of mice subjected to sensitization with hen-egg white lysozyme. Among the principal compounds in IT, apigenin (1), luteolin (3), and luteolin 7-glucoside (4) showed significant allergy-preventive effects.


Asunto(s)
Antialérgicos/uso terapéutico , Glucósidos/farmacología , Hipersensibilidad Tardía/prevención & control , Impatiens/química , Extractos Vegetales/uso terapéutico , Animales , Antialérgicos/farmacología , Apigenina/farmacología , Pollos , Clara de Huevo , Flavonas/farmacología , Flores , Hemorreología/efectos de los fármacos , Hipersensibilidad Tardía/inducido químicamente , Hipersensibilidad Tardía/fisiopatología , Luteolina/farmacología , Ratones , Muramidasa , Fitoterapia , Extractos Vegetales/farmacología , Cola (estructura animal)/irrigación sanguínea
9.
Biol Pharm Bull ; 32(3): 509-12, 2009 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-19252306

RESUMEN

Allergy-preventive activity was demonstrated for the MeOH extract (HM) of the petals of Hibiscus mutabilis L. 'versicolor' MAKINO in a continuing search for allergy-preventive substances from natural sources, using the in vivo assay method. This assay system uses monitoring of a decrease in the blood flow at the tail vein of mice subjected to hen egg-white lysozyme (HEL) sensitization. By bioassay-directed fractionation, a new flavonol triglycoside, quercetin 3-O-[beta-D-xylopyranosyl(1-->2)-alpha-L-rhamnopyranosyl(1-->6)]-beta-D-galactopyranoside (1: mutabiloside), was isolated, together with four known flavonols identified as quercetin 3-O-[beta-D-xylopyranosyl(1-->2)]-beta-D-galactopyranoside (2) and kaempferol 3-O-[beta-D-xylopyranosyl(1-->2)]-beta-D-galactopyranoside (3), quercetin (4) and hyperoside (5). The structure of the new flavonol 1 was elucidated by spectroscopic methods. Among these flavonol derivatives, compounds 1 and 2 showed significant allergy-preventive effects.


Asunto(s)
Antialérgicos/farmacología , Flavonoides/farmacología , Glicósidos/farmacología , Hibiscus/química , Animales , Antialérgicos/aislamiento & purificación , Flavonoides/aislamiento & purificación , Glicósidos/aislamiento & purificación , Masculino , Ratones , Muramidasa/inmunología , Extractos Vegetales/química , Extractos Vegetales/farmacología , Agregación Plaquetaria/efectos de los fármacos , Flujo Sanguíneo Regional/efectos de los fármacos , Cola (estructura animal)/irrigación sanguínea
10.
Biol Pharm Bull ; 30(7): 1324-8, 2007 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-17603174

RESUMEN

Our in vivo assay system developed to search for allergy-preventive substances, assesses the blood flow decrease in tail vein microcirculation of mice subjected to sensitization with hen-egg white lysozyme (HEL). The blood flow decrease appears to be regulated by various factors such as nitric oxide (NO), thromboxane (TX) A(2), prostacyclin (PGI(2)) and endothelin (ET)-1 together with cyclooxygenase (COX)-1, COX-2, inducible nitric oxide synthase (iNOS), and constitutive nitric oxide synthase (cNOS). In this study, we examined in detail the roles of iNOS in this assay system using an iNOS knockout (KO) mouse. We found that the blood flow decrease in the HEL-sensitized iNOS KO mice was slightly weaker than that in their wild type (WT) mice. This blood flow decrease was not affected by a selective COX-1 inhibitor, a selective COX-2 inhibitor and a PGI(2) agonist unlike the case of the WT mice. However, it was inhibited by a nonselective NOS inhibitor, a specific TXA(2) synthase inhibitor and a specific ET-1 receptor blocker as in the case of the WT mice. The present results indicate that the blood flow decrease occurs via two pathways; one is an iNOS-independent response involving TXA(2) and ET-1, and the other is an iNOS-dependent response involving COX-1, COX-2 and PGI(2). cNOS appears to play some roles in the blood flow decrease and iNOS acts as an exacerbation factor. Our method using HEL-sensitized should be useful for searching for agents that can prevent allergy via new mechanisms.


Asunto(s)
Hipersensibilidad/prevención & control , Muramidasa/inmunología , Óxido Nítrico Sintasa de Tipo II/fisiología , Flujo Sanguíneo Regional , Animales , Epoprostenol/análogos & derivados , Epoprostenol/farmacología , Femenino , Metacrilatos/farmacología , Ratones , Ratones Endogámicos C57BL , NG-Nitroarginina Metil Éster/farmacología , Nitrobencenos/farmacología , Péptidos Cíclicos/farmacología , Sulfonamidas/farmacología , Tromboxano A2/biosíntesis , Venas/fisiología
11.
Chem Pharm Bull (Tokyo) ; 55(4): 675-8, 2007 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-17409571

RESUMEN

Allergy-preventive activity was demonstrated for an extract of resins from Xanthorrhoea hastilis R. BR. in a search for allergy-preventive substances from natural sources. By bioassay-directed fractionation of this plant extract, a new flavanone, 3',5'-dihydroxy-7,4'-dimethoxyflavanone (1), and two new chalcones, 3,5,2'-trihydroxy-4,4'-dimethoxychalcone (2) and 5,2'-dihydroxy-3,4,4'-trimethoxychalcone (3), were isolated together with five known compounds, 5'-hydroxy-7,3',4'-trimethoxyflavanone (4), 3'-hydroxy-7,4'-dimethoxyflavanone (5), liquiritigenin 7-methyl ether (6), 4,2'-dihydroxy-4'-methoxychalcone (7) and sakuranetin (8). The structures of 1, 2 and 3 were elucidated by spectroscopic methods. All of these compounds showed allergy-preventive effects.


Asunto(s)
Flavonoides/farmacología , Hipersensibilidad/prevención & control , Magnoliopsida/química , Flavonoides/química , Flavonoides/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Espectrometría de Masa por Ionización de Electrospray , Espectrofotometría Ultravioleta
12.
J Nat Prod ; 69(8): 1215-7, 2006 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-16933880

RESUMEN

Allergy-preventive activity was demonstrated for an extract of the bark of Populus sieboldii in a continuing search for allergy-preventive substances from natural sources. By bioassay-directed fractionation of this plant bark, two new phenolic glycosides, siebolside A {2-hydroxy-5-[(benzoyloxy)methyl]phenyl (6'-O-acetyl) beta-D-glucopyranoside} (1) and siebolside B {2-hydroxy-5-[(benzoyloxy)methyl]phenyl beta-D-glucopyranoside} (2), were isolated, together with three known compounds, salicin (3), sakuranin (4), and neosakuranin (5). The structures of 1 and 2 were elucidated by spectroscopic methods. Compounds 1-5 all showed allergy-preventive effects.


Asunto(s)
Glicósidos , Hipersensibilidad/prevención & control , Populus/química , Animales , Plaquetas/efectos de los fármacos , Glucósidos , Glicósidos/sangre , Glicósidos/química , Glicósidos/aislamiento & purificación , Glicósidos/farmacología , Japón , Ratones , Estructura Molecular , Fenoles/sangre , Fenoles/química , Fenoles/aislamiento & purificación , Fenoles/farmacología , Corteza de la Planta/química
13.
Neurosci Lett ; 394(3): 206-10, 2006 Feb 20.
Artículo en Inglés | MEDLINE | ID: mdl-16260090

RESUMEN

In the course of our survey of natural compounds inhibiting prostaglandin E2 release and/or lipopolysaccharide (LPS)-induced transcriptional stimulation via NF-kappaB, a central regulator of inflammatory genes, from natural resources, we found garcinone B, a xanthone from callus tissue culture of Hypericum patulum, as a compound with such pharmacological activities, that is a derivative of gamma-mangostin which potently inhibits COX-1 and COX-2 activities to reduce PGE2 release from C6 rat glioma cells, and inhibits IKK activity to prevent NF-kappaB-dependent COX-2 gene transcription. Garcinone B, to a lesser extent, reduced A23187-induced increase in prostaglandin E2 release than gamma-mangostin and its structurally related compound, patulone, in C6 cells. This compound also prevented LPS-induced stimulation of NF-kappaB-dependent transcription. These results suggest that garcinone B becomes a unique pharmacological tool to investigate intracellular signaling pathways involved in inflammation.


Asunto(s)
Neoplasias Encefálicas/metabolismo , Dinoprostona/metabolismo , Glioma/metabolismo , FN-kappa B/genética , Antagonistas de Prostaglandina/farmacología , Transcripción Genética/efectos de los fármacos , Xantonas/farmacología , Animales , Calcimicina/farmacología , Línea Celular Tumoral , Ciclooxigenasa 1/metabolismo , Hypericum/química , Mediadores de Inflamación/metabolismo , Lipopolisacáridos/farmacología , Ratas , Xantinas/farmacología
14.
Biol Pharm Bull ; 28(9): 1786-90, 2005 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-16141562

RESUMEN

The anti-anaphylactic and anti-pruritic activities of a 35% EtOH extract (IT) of the flowers of Impatiens textori MIQ. were investigated by in vivo assay. IT and apigenin (1), apigenin 7-glucoside (2) and luteolin (3), principal compounds from IT, inhibited compound 48/80 (COM)-induced by blood pressure (BP) decrease, which was an immunoglobulin (Ig)E-independent anaphylaxis-like response. Compounds 1-3 all inhibited BP decrease induced by IgE-dependent anaphylaxis. Furthermore, IT also inhibited the blood flow (BF) decrease induced by antigen-induced anaphylaxis in actively sensitized mice. IT showed a significant inhibitory effect on scratching behavior induced by COM without a central depressant. IT also significantly inhibited platelet activating factor (PAF)- and serotonin (5-HT)-induced scratching behavior and mitigated protease (PA)-induced scratching behavior. These findings showed that the flowers of I. textori can be utilized as an anti-anaphylactic and anti-pruritic agent in addition to the traditional applications of this plant.


Asunto(s)
Anafilaxia/tratamiento farmacológico , Impatiens/química , Prurito/tratamiento farmacológico , Animales , Conducta Animal/efectos de los fármacos , Presión Sanguínea/efectos de los fármacos , Flores/química , Hipnóticos y Sedantes/farmacología , Inmunoglobulina E/inmunología , Masculino , Ratones , Actividad Motora/efectos de los fármacos , Muramidasa/inmunología , Factor de Activación Plaquetaria/farmacología , Prurito/psicología , Flujo Sanguíneo Regional/efectos de los fármacos , Serotonina/farmacología , p-Metoxi-N-metilfenetilamina/farmacología
15.
Biol Pharm Bull ; 28(8): 1490-5, 2005 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-16079499

RESUMEN

We discovered a phenomenon in which the blood flow in vein microcirculation markedly decreases in response to hen-egg white lysozyme (HEL)-sensitization without any change in blood pressure. Using this blood flow decrease as a guide, we developed an in vivo assay method to search for substances, which can prevent allergies. Antagonists of histamine, serotonin and platelet activating factor (PAF) did not affect the blood flow decrease in response to HEL-sensitization. On the other hand, cyclooxygenase (COX)-1, COX-2, thromboxane (TX) A(2), endothelin-1 (ET-1), prostacyclin (PGI(2)) and granulocytic elastase (GE) as well as nitric oxide (NO) from inducible NO synthase (iNOS) were involved in the blood flow decrease. Thus, these substances might injure vascular endothelial cells, and cause a decrease in blood flow in vein microcirculation. Our method can be used to search for preventive agents against allergies involving NO, COX-1, 2 and PGI(2). This is the first report to applying to an assay method the specific blood flow decrease to occur in the promotion stage of allergy.


Asunto(s)
Antialérgicos/farmacología , Bioensayo , Muramidasa/farmacología , Flujo Sanguíneo Regional/efectos de los fármacos , Animales , Clara de Huevo , Ratones , Óxido Nítrico/biosíntesis
16.
Planta Med ; 71(1): 90-2, 2005 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-15678383

RESUMEN

The inhibitory effects of 22 xanthones from three Guttiferae plants (Hypericum patulum, Calophyllum inophyllum and C. austroindium) on exogenous platelet activating factor (PAF)-induced hypotension were examined using a blood pressure monitoring in vivo assay method. Guanandin (2), caloxanthone E (3), 1,3,5,6-tetrahydroxy-2-isoprenylxanthone (8), 6-deoxyjacareubin (11) and patulone (18) showed strong inhibition of PAF-induced hypotension, with inhibitory effects of more than 60 %. Their ID50 values were greater than that of ginkgolide B (BN-52 021), a natural PAF-antagonist from the Ginkgo biloba.


Asunto(s)
Antihipertensivos/farmacología , Calophyllum , Hypericum , Fitoterapia , Extractos Vegetales/farmacología , Animales , Antihipertensivos/administración & dosificación , Antihipertensivos/uso terapéutico , Presión Sanguínea/efectos de los fármacos , Hipertensión/inducido químicamente , Hipertensión/tratamiento farmacológico , Masculino , Ratones , Ratones Endogámicos , Extractos Vegetales/administración & dosificación , Extractos Vegetales/uso terapéutico , Factor de Activación Plaquetaria
17.
J Pharmacol Sci ; 95(1): 33-40, 2004 May.
Artículo en Inglés | MEDLINE | ID: mdl-15153648

RESUMEN

We investigated the cell death effects of eight xanthones on PC12 rat pheochromocytoma cells. Among these compounds, alpha-mangostin, from the fruit hull of Garcinia mangostana L., had the most potent effect with the EC(50) value of 4 microM. Alpha-mangostin-treated PC12 cells demonstrated typical apoptotic DNA fragmentation and caspase-3 cleavage (equivalent to activation). The flow cytometric analysis indicated that this compound induced apoptosis in time-and concentration-dependent manners. Alpha-mangostin showed the features of the mitochondrial apoptotic pathway such as mitochondrial membrane depolarization and cytochrome c release. Furthermore, alpha-mangostin inhibited the sarco(endo)plasmic reticulum Ca(2+)-ATPase markedly. There was a correlation between the Ca(2+)-ATPase inhibitory effects and the apoptotic effects of the xanthone derivatives. On the other hand, c-Jun NH(2)-terminal kinase (JNK/SAPK), one of the signaling molecules of endoplasmic reticulum (ER) stress, was activated with alpha-mangostin treatment. These results suggest that alpha-mangostin inhibits Ca(2+)-ATPase to cause apoptosis through the mitochondrial pathway.


Asunto(s)
Apoptosis/efectos de los fármacos , ATPasa de Ca(2+) y Mg(2+)/metabolismo , Mitocondrias/efectos de los fármacos , Xantonas/farmacología , Animales , Apoptosis/fisiología , Supervivencia Celular/efectos de los fármacos , Supervivencia Celular/fisiología , Relación Dosis-Respuesta a Droga , Mitocondrias/enzimología , Células PC12 , Ratas
18.
Biol Pharm Bull ; 26(10): 1505-7, 2003 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-14519965

RESUMEN

A 35% EtOH extract of flowers of Impatiens textori MIQ. showed an inhibitory effect on blood pressure decrease in response to platelet activating factor (PAF) measured with a blood pressure monitoring system. Bioassay-guided fractionation of the 35% EtOH extract (IT) led to isolation of the flavones apigenin (1) and luteolin (3), which significantly inhibited blood pressure decrease in response to PAF. Their compounds and apigenin 7-glucoside (2), chrysoeriol (4), quercetin (5), quercetin 3-glucoside (6), kaempferol (7), kaempferol 3-glucoside (8) and kaempferol 3-rhamnosyldiglucoside (9) were also isolated from the flowers of I. textori for the first time. This study revealed that the flowers of I. textori might be a possible anti-allergy agent.


Asunto(s)
Presión Sanguínea/efectos de los fármacos , Impatiens , Factor de Activación Plaquetaria/farmacología , Animales , Presión Sanguínea/fisiología , Flores , Masculino , Ratones , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/farmacología
19.
Biol Pharm Bull ; 26(7): 1031-4, 2003 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-12843634

RESUMEN

A 35% EtOH extract of the fruits of Chaenomeles sinensis, long utilized as a folk medicine for cough, significantly inhibited the pruritogenic agent compound 48/80 (COM)-induced scratching behavior in mice. Antipruritic activity-guided fractionation and purification yielded active quercetin, apigenin, and catechin derivatives, which exhibited significant inhibitory effects on COM-induced scratching behavior. To the best of our knowledge, apigenin (5), apigenin 7-glucronide (6), and apigenin 4'-methoxy-7-glucronide (acacetin 7-glucronide) (7) were isolated from the fruits of C. sinensis for the first time. The active fraction and these compounds also inhibited serotonin-, platelet activating factor-, and prostaglandin E(2)-induced scratching behavior, but did not inhibit histamine-induced scratching behavior or locomotive behavior. This study also showed that the fruits of C. sinensis could be used to treat allergic itching sensation.


Asunto(s)
Antipruriginosos/uso terapéutico , Frutas , Prurito/tratamiento farmacológico , Rosaceae , Animales , Antipruriginosos/química , Antipruriginosos/farmacología , Locomoción/efectos de los fármacos , Locomoción/fisiología , Masculino , Ratones , Ratones Endogámicos ICR , Fitoterapia/métodos , Extractos Vegetales/química , Extractos Vegetales/farmacología , Extractos Vegetales/uso terapéutico , Prurito/inducido químicamente
20.
Biol Pharm Bull ; 25(12): 1655-7, 2002 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-12499660

RESUMEN

The effects of conjugated linoleic acid (CLA) against anaphylaxis and allergic pruritus were investigated using a in vivo assay. Inhibitory effects of CLA were observed on the immediate (type 1) hypersensitivity reaction, with CLA significantly suppressing the decrease in blood pressure (BP) and blood flow (BF) induced by the hen egg-white lysozyme (HEL)-anaphylactic reaction in ddY mice. After oral administration, CLA showed antipruritic activity, with significant inhibition of scratching behavior induced by compound 48/80 (COM), a histamine-release agent. When painted onto the skin, CLA also inhibited COM, platelet-activating factor, and protease-induced scratching behavior, and COM-induced vasodilation of the skin. CLA offers promise as a drug for the treatment of allergic and inflammatory pruritus not only as an oral but also a topical agent. The present findings demonstrate that CLA can be effective for the prevention and treatment of allergic disease with severe pruritus.


Asunto(s)
Anafilaxia/tratamiento farmacológico , Ácido Linoleico/uso terapéutico , Prurito/tratamiento farmacológico , Anafilaxia/inducido químicamente , Anafilaxia/fisiopatología , Animales , Masculino , Ratones , Prurito/inducido químicamente , Prurito/fisiopatología , Ratas , Ratas Wistar
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