RESUMEN
Leukemia-associated antigens such as proteins encoded by MAGE genes might provide tools for immunotherapy of leukemia. Positive and negative results of MAGE-A gene expression in hematological malignancies have been reported. This led us to study MAGE-A gene expression in human leukemias using RT-PCR. Among 115 leukemias from various subtypes, 14/34 (41.17%) AML were positive for one of the three genes analyzed (MAGE-A1 1/32; MAGE-A3 10/32; MAGE-B2 3/12). Expression was also detected in 23/76 (30.26%) B-cell ALL patients (MAGE-A1 2/53; MAGE-A3 20/53; MAGE-B2 1/32). One of these patients expressed both MAGE-A1 (weak signal) and -A3 (strong signal) genes. Other patient with CML were positive for MAGE-B2 (1/5, 20%). MAGE-A3 expression data were corroborated by real time RT-PCR through determination of MAGE-A3 transcript levels. We concluded that the MAGE-A3 gene is expressed at the mRNA level in a proportion of human leukemias.
Asunto(s)
Antígenos de Neoplasias/genética , Leucemia/genética , Proteínas de Neoplasias/genética , ARN Mensajero/genética , Transcripción Genética , Adulto , Antígenos de Neoplasias/sangre , Secuencia de Bases , Cartilla de ADN , Femenino , Amplificación de Genes , Humanos , Leucemia/sangre , Leucemia Mielógena Crónica BCR-ABL Positiva/genética , Leucemia Mieloide Aguda/genética , Masculino , Persona de Mediana Edad , Proteínas de Neoplasias/sangre , Leucemia-Linfoma Linfoblástico de Células Precursoras/genética , Reacción en Cadena de la Polimerasa de Transcriptasa InversaAsunto(s)
Antibacterianos/farmacología , Resistencia a Múltiples Medicamentos , Macrólidos , Streptococcus agalactiae/efectos de los fármacos , Virginiamicina/farmacología , Hemólisis , Humanos , Lincosamidas , Pruebas de Sensibilidad Microbiana , Fenotipo , España , Streptococcus agalactiae/fisiologíaAsunto(s)
Enfermedades Transmisibles , Laboratorios/normas , Microbiología , Sociedades Médicas , Humanos , Control de Calidad , EspañaRESUMEN
BACKGROUND: To study the characteristics of bite wounds with unfavorable evolution, developing infectious complications that requires hospital admission. METHODS: The data from 22 patients admitted to the Ciudad Sanitaria Vall d'Hebron hospital for the above mentioned reason over the last 5 years were reviewed. RESULTS: The patients (8 males, 8 females and 6 children) were bitten by 10 dogs, 6 cats, and 6 men with predominance of the wound site being in the upper limb (10) followed by the lower limbs (6), head or face (5) and exceptionally on the breast (1). The most frequent clinical manifestation was abscess and/or cellulitis (13) and adenopathies or lymphangitis (4); 5 patients presented osteoarticular involvement including 3 bone fractures due to human aggression. With regard to the etiology of infection, the common bucal flora bacteria were isolated in all the cases; Pasteurella multocida in 15/16 animal bites, Eikenella corrodens associated to streptococcus in 5/6 human bites, Fusobacterium spp. (5), Bacteroides spp. (3) and Peptococcus sp. (1). The most frequently administered antibiotics were gentamycin (15), penicillin (13), cloxacillin (5) and clindamycin (4). The evolution was favorable, although slow in many cases, with sequelae in 3 patients. CONCLUSIONS: It is very difficult to foresee in which cases infectious complications will develop in bite wounds. According to the authors' experience, in the case of deep wounds the bacteria implied come from the mouth of the aggressor. Careful cleansing, rapid administration of an adequate antibiotic and clinical control being the most recommendable procedure.