New Insights into the Antimicrobial Potential of Polyalthia longifolia-Antibiofilm Activity and Synergistic Effect in Combination with Penicillin against Staphylococcus aureus.

Widespread antibiotic resistance has led to the urgent need for the identification of new antimicrobials. Plants are considered a valuable potential resource for new effective antimicrobial compounds. Therefore, in the present study, we focused on the antimicrobial activity of Polyalthia longifolia plants harvested from Cameroon using the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and time-kill assays. The mechanism of action was investigated by employing fluorescence and scanning electron microscopy. The anti-Staphylococcus aureus activity was studied using biofilm inhibition and checkerboard assays. Our results revealed that the tested extracts possess important antimicrobial activities, notably against Gram positive bacteria (MICs as low as 0.039 mg/mL). P. longifolia leaf extracts exhibited a significant bactericidal effect, with a total kill effect recorded after only 2 h of exposure at concentrations equivalent to MBC (0.078 and 0.156 mg/mL). The extracts showed a synergistic antibacterial activity in combination with penicillin against a MRSA clinical isolate and significantly inhibited S. aureus biofilm formation. The mechanism of action is related to the impairment of cell membrane integrity and cell lysis. All these findings suggest that P. longifolia could be an important source of reliable compounds used to develop new antimicrobials.

Assessment of the Fertilization Capacity of the Aquaculture Sediment for Wheat Grass as Sustainable Alternative Use.

Periodic removal of sediment from aquaculture ponds is practiced to maintain their productivity and animal welfare. The recovery of sediment as a plant fertilizer could alleviate the costs of sediment removal. The objective of this study was to test the effects of a dried sediment, extracted from an aquaculture pond used for common carp cultivation, on the growth and physiology of potted wheat grass and the quality of the juice obtained from wheat grass. The results showed that sediment application did not produce significant morphological changes, although the values for plant height (16.94-19.22 cm), leaf area (19.67-139.21 mm2), and biomass (3.39-4.26 g/plant) were higher in sediment-grown plants. However, at a physiological level, the effect was negative, decreasing photosynthesis (0.82-1.66 µmol CO2 m2s-1), fluorescence ΦPSII (0.737-0.782), and chlorophyll content (1.40-1.83 CCI). The juice yield was reduced in the sediment treatments (46-58 g/100 g), while the quality was improved by increasing the content of phenols (2.55-3.39 µg/mL gallic acid equivalent), flavonoids (1.41-1.85 µg/mL quercetin equivalent), and antioxidant activity (47.99-62.7% inhibition of; 2,2-diphenyl-1-picrylhydrazyl). The positive results obtained in this study can be attributed to the moderate nutrient content of the sediment and a negligible concentration of heavy metals.