RESUMEN
Fungicides should be used to the extent required to minimize economic costs of disease in a given field in a given season. The maximum number of treatments and maximum dose per treatment are set by fungicide manufacturers and regulators at a level that provides effective control under high disease pressure. Lower doses are economically optimal under low or moderate disease pressure, or where other control measures such as resistant cultivars constrain epidemics. Farmers in many countries often apply reduced doses, although they may still apply higher doses than the optimum to insure against losses in high disease seasons. Evidence supports reducing the number of treatments and reducing the applied dose to slow the evolution of fungicide resistance. The continuing research challenge is to improve prediction of future disease damage and account for the combined effect of integrated control measures to estimate the optimum number of treatments and the optimum dose needed to minimize economic costs. The theory for optimizing dose is well developed but requires translation into decision tools because the current basis for farmers' dose decisions is unclear.
Asunto(s)
Agricultura/métodos , Fungicidas Industriales/administración & dosificación , Enfermedades de las Plantas/prevención & controlRESUMEN
Parastagonospora nodorum is a necrotrophic fungal pathogen that causes Septoria nodorum blotch (SNB) (formerly Stagonospora nodorum blotch) on wheat. P. nodorum produces necrotrophic effectors (NE) that are recognized by dominant host sensitivity gene products resulting in disease development. The NE-host interaction is critical to inducing NE-triggered susceptibility (NETS). To date, seven NE-host sensitivity gene interactions, following an inverse gene-for-gene model, have been identified in the P. nodorum-wheat pathosystem. Here, we used a wheat mapping population that segregated for sensitivity to two previously characterized interactions (SnTox1-Snn1 and SnTox3-Snn3-B1) to identify and characterize a new interaction involving the NE designated SnTox6 and the host sensitivity gene designated Snn6. SnTox6 is a small secreted protein that induces necrosis on wheat lines harboring Snn6. Sensitivity to SnTox6, conferred by Snn6, was light-dependent and was shown to underlie a major disease susceptibility quantitative trait locus (QTL). No other QTL were identified, even though the P. nodorum isolate used in this study harbored both the SnTox1 and SnTox3 genes. Reverse transcription-polymerase chain reaction showed that the expression of SnTox1 was not detectable, whereas SnTox3 was expressed and, yet, did not play a significant role in disease development. This work expands our knowledge of the wheat-P. nodorum interaction and further establishes this system as a model for necrotrophic specialist pathosystems.
Asunto(s)
Ascomicetos/genética , Proteínas Fúngicas/metabolismo , Interacciones Huésped-Patógeno , Enfermedades de las Plantas/microbiología , Proteínas de Plantas/metabolismo , Triticum/microbiología , Ascomicetos/fisiología , Mapeo Cromosómico , Susceptibilidad a Enfermedades , Proteínas Fúngicas/genética , Modelos Genéticos , Micotoxinas/genética , Micotoxinas/metabolismo , Proteínas de Plantas/genética , Mapeo de Interacción de Proteínas , Sitios de Carácter Cuantitativo/genética , Triticum/genética , Triticum/metabolismoRESUMEN
A fungicide resistance model (reported and tested previously) was amended to describe the development of resistance in Mycosphaerella graminicola populations in winter wheat (Triticum aestivum) crops in two sets of fields, connected by spore dispersal. The model was used to evaluate the usefulness of concurrent, alternating, or mixture use of two high-resistance-risk fungicides as resistance management strategies. We determined the effect on the usefulness of each strategy of (i) fitness costs of resistance, (ii) partial resistance to fungicides, (iii) differences in the dose-response curves and decay rates between fungicides, and (iv) different frequencies of the double-resistant strain at the start of a treatment strategy. Parameter values for the quinine outside inhibitor pyraclostrobin were used to represent two fungicides with differing modes of action. The effectiveness of each strategy was quantified as the maximum number of growing seasons that disease was effectively controlled in both sets of fields. For all scenarios, the maximum effective lives achieved by the use of the strategies were in the order mixtures ≥ alternation ≥ concurrent use. Mixtures were of particular benefit where the pathogen strain resistant to both modes of action incurred a fitness penalty or was present at a low initial frequency.
Asunto(s)
Ascomicetos/efectos de los fármacos , Farmacorresistencia Fúngica/efectos de los fármacos , Fungicidas Industriales/farmacología , Enfermedades de las Plantas/prevención & control , Triticum/microbiología , Carbamatos/farmacología , Simulación por Computador , Relación Dosis-Respuesta a Droga , Modelos Biológicos , Enfermedades de las Plantas/microbiología , Pirazoles/farmacología , Estaciones del Año , EstrobilurinasRESUMEN
The wheat disease tan (or yellow leaf) spot, caused by Pyrenophora tritici-repentis, was first described in the period 1934 to 1941 in Canada, India, and the United States. It was first noted in Australia in 1953 and only became a serious disease in the 1970s. The emergence of this disease has recently been linked to the acquisition by P. tritici-repentis of the ToxA gene from the wheat leaf and glume blotch pathogen, Stagonospora nodorum. ToxA encodes a host-specific toxin that interacts with the product of the wheat gene Tsn1. Interaction of ToxA with the dominant allele of Tsn1 causes host necrosis. P. tritici-repentis races lacking ToxA give minor indistinct lesions on wheat lines, whereas wheat lines expressing the recessive tsn1 are significantly less susceptible to the disease. Although the emergence and spread of tan spot had been attributed to the adoption of minimum tillage practices, we wished to test the alternative idea that the planting of Tsn1 wheat lines may have contributed to the establishment of the pathogen in Australia. To do this, wheat cultivars released in Australia from 1911 to 1986 were tested for their sensitivity to ToxA. Prior to 1941, 16% of wheat cultivars were ToxA-insensitive and hence, all other factors being equal, would be more resistant to the disease. Surprisingly, only one of the cultivars released since 1940 was ToxA insensitive, and the area planted to ToxA-insensitive cultivars varied from 0 to a maximum of only 14% in New South Wales. Thus, the majority of the cultivars were ToxA-sensitive both before and during the period of emergence and spread of the disease. We therefore conclude that the spread of P. tritici-repentis in Australia cannot be causally linked to the deployment of ToxA-sensitive cultivars.
Asunto(s)
Ascomicetos/genética , Micotoxinas/metabolismo , Enfermedades de las Plantas/genética , Proteínas de Plantas/genética , Triticum/genética , Ascomicetos/metabolismo , Ascomicetos/fisiología , Australia , Interacciones Huésped-Patógeno , Enfermedades de las Plantas/microbiología , Hojas de la Planta/genética , Hojas de la Planta/microbiología , Proteínas de Plantas/metabolismo , Triticum/microbiologíaRESUMEN
The world's oldest and largest Medicago truncatula collection is housed at the South Australian Research and Development Institute (SARDI). We used six simple sequence repeat (SSR) loci to analyse the genetic diversity and relationships between randomly selected individuals from 192 accessions in the core collection. M. truncatula is composed of three subspecies (ssp.): ssp. truncatula, ssp. longeaculeata, and ssp. tricycla. Analysis at the level of six SSR loci supports the concept of ssp. tricycla, all the samples of which showed unique alleles at two loci. Contingency Chi-squared tests were significant between ssp. tricycla and ssp. truncatula at four loci, suggesting a barrier to gene flow between these subspecies. In accessions defined as ssp. longeaculeata, no unique allelic distribution or diagnostic sizes were observed, suggesting this apparent ssp. is a morphological variant of ssp. truncatula. The data also suggest M. truncatula that exhibits unusually wide genotype dispersal throughout its native Mediterranean region, possibly due to animal and trade-related movements. Our results showed the collection to be highly diverse, exhibiting an average of 25 SSR alleles per locus, with over 90% of individuals showing discrete genotypes. The rich diversity of the SARDI collection provides an invaluable resource for studying natural allelic variation of M. truncatula. To efficiently exploit the variation in the SARDI collection, we have defined a subset of accessions (n = 61) that maximises the diversity.
Asunto(s)
Variación Genética , Genotipo , Medicago truncatula/genética , Repeticiones de Microsatélite , Alelos , Animales , Geografía , Medicago truncatula/clasificación , Mar Mediterráneo , Filogenia , Distribución AleatoriaRESUMEN
Powdery mildew of barley is caused by the obligate fungal pathogen Blumeria graminis f. sp. hordei. Haploid conidia of B. graminis, landing on the barley leaf, germinate to form first a primary germ tube and then an appressorial germ tube. The appressorial germ tube differentiates into a mature appressorium from which direct penetration of host epidermis occurs. Here we present data on 4908 expressed sequence tags obtained from B. graminis conidia. The combined sequences represent 2676 clones describing 1669 individual genes. Comparison with sequences from other pathogenic and nonpathogenic fungi defines hypotheses on the genes required for pathogenicity and growth on the host. The putative roles of some of the identified genes are discussed.
Asunto(s)
Ascomicetos/genética , Etiquetas de Secuencia Expresada , Genes Fúngicos , Hordeum/microbiología , Enfermedades de las Plantas/microbiología , Ascomicetos/patogenicidad , Ascomicetos/fisiología , Clonación Molecular , Codón , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Biblioteca de Genes , Datos de Secuencia MolecularRESUMEN
To address the problem of the nutritional requirements of phyto-pathogenic fungi growing in planta, the environment for the intercellular biotrophic pathogen, Cladosporium fulvum Cooke, of tomato (Lycopersicon esculentum Mill.) was analysed. Using a novel technique for infiltrating the intercellular space, we measured the concentrations of 21 amino acids, nitrate and ammonia in the apoplast of the tomato leaf during infection. The concentrations of most amino acids, and total nitrogen content, increased during infection. The levels of nearly all amino acids remained relatively unchanged during an incompatible interaction. All protein amino acids were detected during infection, except cysteine and tryptophan. Most amino acids were present at a concentration between 0.1-0.7 mM. The non-protein amino acid gamma-aminobutyric acid was detected at the highest concentration (up to 2.5 mM) during the compatible interaction. Preliminary investigations on the source of the amino acids revealed that protease activity within the apoplast increased during infection and that infection induced the expression of the pathogenicity-related extracellular serine protease P69B. The nitrogen status of the infecting fungus and sources for the additional amino acids are discussed.
Asunto(s)
Cladosporium/patogenicidad , Nitrógeno/metabolismo , Enfermedades de las Plantas , Solanum lycopersicum/microbiología , Adaptación Fisiológica , Aminoácidos/metabolismo , Amoníaco/metabolismo , Pared Celular/enzimología , Pared Celular/metabolismo , Pared Celular/microbiología , Endopeptidasas/metabolismo , Regulación de la Expresión Génica de las Plantas , Solanum lycopersicum/enzimología , Solanum lycopersicum/metabolismo , Modelos Biológicos , Nitratos/metabolismo , Hojas de la Planta/enzimología , Hojas de la Planta/metabolismo , Hojas de la Planta/microbiología , Ácido gamma-Aminobutírico/metabolismoRESUMEN
C. fulvum, a fungal tomato pathogen, has previously been shown to express a complex family of hydrophobin genes including four class I hydrophobins and one class II hydrophobin. Here we describe a gene for HCf-6, a sixth member of the hydrophobin family and the second class II gene. The protein is predicted to consist of a signal sequence, an N-terminus rich in glycine and asparagine and a C-terminal hydrophobic domain which bears the hall-marks of hydrophobins. In contrast to the previously described class II hydrophobin HCf-5, HCf-6 is expressed in mycelium growing in pure culture and mRNA levels do not increase during sporulation. It is down-regulated by carbon starvation but not by depletion of nitrogen in the growth medium.
Asunto(s)
Cladosporium/genética , Proteínas Fúngicas/genética , Secuencia de Aminoácidos , Secuencia de Bases , Cladosporium/química , Cladosporium/metabolismo , ADN de Hongos/química , ADN de Hongos/genética , ADN de Hongos/aislamiento & purificación , Proteínas Fúngicas/química , Proteínas Fúngicas/aislamiento & purificación , Regulación Fúngica de la Expresión Génica , Genes Fúngicos , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Filogenia , Reacción en Cadena de la Polimerasa , ARN de Hongos/química , ARN de Hongos/genética , ARN de Hongos/aislamiento & purificación , ARN Mensajero/biosíntesis , ARN Mensajero/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Propiedades de SuperficieRESUMEN
Cladosporiumfulvum is a mitosporic ascomycete pathogen of tomato. A study of fungal genes expressed during carbon starvation in vitro identified several genes that were up regulated during growth in planta. These included genes predicted to encode acetaldehyde dehydrogenase (Aldh1) and alcohol oxidase (Aox1). An Aldh1 deletion mutant was constructed. This mutant lacked all detectable ALDH activity, had lost the ability to grow with ethanol as a carbon source, but was unaffected in pathogenicity. Aox1 expression was induced by carbon starvation and during the later stages of infection. The alcohol oxidase enzyme activity has broadly similar properties (Km values, substrate specificity, pH, and heat stability) to yeast enzymes. Antibodies raised to Hansenula polymorpha alcohol oxidase (AOX) detected antigens in Western blots of starved C. fulvum mycelium and infected plant material. Antigen reacting with the antibodies was localized to organelles resembling peroxisomes in starved mycelium and infected plants. Disruption mutants of Aox1 lacked detectable AOX activity and had markedly reduced pathogenicity as assayed by two different measures of fungal growth. These results identify alcohol oxidase as a novel pathogenicity factor and are discussed in relation to peroxisomal metabolism of fungal pathogens during growth in planta.
Asunto(s)
Oxidorreductasas de Alcohol/metabolismo , Aldehído Deshidrogenasa/metabolismo , Cladosporium/patogenicidad , Proteínas Fúngicas/metabolismo , Isoenzimas/metabolismo , Oxidorreductasas de Alcohol/genética , Aldehído Deshidrogenasa/genética , Familia de Aldehído Deshidrogenasa 1 , Secuencia de Aminoácidos , Northern Blotting , Southern Blotting , Western Blotting , Cladosporium/enzimología , Cladosporium/genética , ADN de Hongos/análisis , Electroforesis en Gel de Poliacrilamida , Proteínas Fúngicas/genética , Isoenzimas/genética , Solanum lycopersicum/microbiología , Datos de Secuencia Molecular , Mutagénesis , Peroxisomas/enzimología , Peroxisomas/metabolismo , Peroxisomas/ultraestructura , ARN de Hongos/análisis , Retinal-Deshidrogenasa , Alineación de Secuencia , Análisis de Secuencia de ADN , VirulenciaRESUMEN
Obligate plant-pathogenic fungi have proved extremely difficult to characterize with molecular genetics because they cannot be cultured away from host plants and only can be manipulated experimentally in limited circumstances. Previously, in order to characterize signal transduction processes during infection-related development of the powdery mildew fungus Blumeria graminis (syn. Erysiphe graminis) f. sp. hordei, we described a gene similar to the catalytic subunit of cyclic AMP-dependent protein kinase A (here renamed Bka1). Functional characterization of this gene has been achieved by expression in a deltacpkA mutant of the nonobligate pathogen Magnaporthe grisea. This nonpathogenic M. grisea deltacpkA mutant displays delayed and incomplete appressorium development, suggesting a role for PKA-c in the signal transduction processes that control the maturation of infection cells. Transformation of the deltacpkA mutant with the mildew Bka1 open reading frame, controlled by the M. grisea MPG1 promoter, restored pathogenicity and appressorium maturation kinetics. The results provide, to our knowledge, the first functional genetic analysis of pathogenicity in an obligate pathogen and highlight the remarkable conservation of signaling components regulating infection-related development in pathogenic fungi.
Asunto(s)
Proteínas Arqueales , Ascomicetos/genética , Proteínas Quinasas Dependientes de AMP Cíclico/genética , Prueba de Complementación Genética , Magnaporthe/genética , Chaperonas Moleculares/genética , Mutación , Plantas/microbiología , Ascomicetos/fisiología , Cartilla de ADN , Hordeum/microbiología , Magnaporthe/fisiologíaRESUMEN
Abstract The nutritional requirements of phytopathogenic fungi growing in planta has to date been largely ignored. We have begun to address this problem by investigating the methionine requirement for the biotrophic pathogen of tomato Cladosporium fulvum during infection. The Met6 gene from Cladosporium fulvum encoding a cobalamin-independent 5-methyltetrahydropteroyltriglutamate-homocysteinemethyltransferase, was cloned by functional yeast complementation. The open reading frame was found to be 2304 bp, containing no introns and encoding a protein of 87 kDa. In vitro Northern analysis demonstrated high levels of Met6 expression in the absence of externally supplied methionine. However in the presence of methionine or in the absence of carbon, expression of Met6 decreased significantly. Analysis of Met6 expression in planta revealed a strong increase during infection suggesting the requirement for methionine synthesis in planta by Cladosporium fulvum. This study demonstrates that Cladosporium fulvum is starving for methionine during infection and thus implies the essentiality of primary biosynthetic pathways to the infecting fungus.
RESUMEN
Erysiphe graminis f. sp. hordei, the causal agent of barley powdery mildew, is an obligate biotroph. On arrival on the host, a primary germ tube (PGT) emerges from the conidium. An appressorial germ tube (AGT) then appears, forms an appressorium, and effects host penetration. Such developmental precision may be due to multiple, plant-derived signals and to endogenous tactile and chemical signals. The transduction mechanism remains obscure. The isolation of an expressed sequence tag (EST) homologue of the catalytic subunit of cyclic AMP (cAMP)-dependent protein kinase A (PKA) enabled the corresponding gene to be characterized and the transcript to be identified in conidia and in PGT and AGT stage spores. cAMP-dependent PKA activity was detected in ungerminated conidia. These data suggest that PKA and cAMP are involved in conidial development. To substantiate this we exploited the responses of developing conidia to various surfaces, including exposure to the host leaf (fully inductive to AGT formation), cellulose membrane (semi-inductive), and glass (non-inductive). Assessment of fungal development, following application of exogenous cAMP or cAMP analogues, revealed that, at different concentrations and on different surfaces, cAMP either promoted or inhibited conidial differentiation. Various PKA inhibitors were tested for their effect on PKA activity and conidial development. A negative correlation was established between PKA inhibition in vitro and fungal development in vivo. Taken collectively, these data suggest that PKA and cAMP play a role in conidial differentiation in this obligate, plant-pathogenic fungus.
Asunto(s)
Ascomicetos/crecimiento & desarrollo , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , AMP Cíclico/metabolismo , Secuencia de Aminoácidos , Ascomicetos/enzimología , Ascomicetos/metabolismo , Secuencia de Bases , Dominio Catalítico , Proteínas Quinasas Dependientes de AMP Cíclico/genética , Cartilla de ADN , Inhibidores Enzimáticos/farmacología , Datos de Secuencia Molecular , Homología de Secuencia de AminoácidoRESUMEN
Five different hydrophobin-encoding cDNA clones from Cladosporium fulvum were isolated from cDNA libraries, made from nutrient-depleted mycelium. One cDNA clone was identical to the previously isolated hydrophobin HCf-1. The other clones were named HCf-2, -3, -4 and -5. HCf-1, -2, -3 and -4 show a high degree of identity, and are predicted to encode class I hydrophobins. HCf-5 encodes a class II hydrophobin. The expression patterns of these hydrophobins at various stages of development, and in liquid media lacking either carbon or nitrogen, or both, showed clear differences. All hydrophobins were more strongly expressed during sporulation than before, with HCf-4 and -5 showing the highest increase. Expression of HCf genes in infected plants was also higher at 16 days than at 10 days after infection. The expression of HCf-5 in sporulating mycelium was much lower in planta than in vitro. All HCf genes were upregulated under conditions of nutrient deprivation. HCf-1, -2, -3 and -4 showed highest levels of transcription in medium lacking both carbon and nitrogen. Expression of HCf-5 was highest in medium lacking nitrogen but containing carbon. HCf-1 was generally the most abundant hydrophobin. The introduction of multiple copies of HCf-1, which caused co-suppression of the endogenous HCf-1 gene, was shown to affect the expression of HCf-2, -3 and -4 also. Expression of HCf-4 was suppressed, but expression of HCf-2 and -3 was upregulated. Expression of HCf-5 was not changed.
Asunto(s)
Cladosporium/genética , Proteínas Fúngicas/genética , Regulación Fúngica de la Expresión Génica , Solanum lycopersicum/microbiología , Secuencia de Aminoácidos , Secuencia de Bases , Cladosporium/patogenicidad , Cladosporium/fisiología , Clonación Molecular , ADN Complementario , Proteínas Fúngicas/biosíntesis , Proteínas Fúngicas/química , Datos de Secuencia Molecular , Filogenia , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Esporas Fúngicas , Factores de TiempoRESUMEN
The nematode-trapping fungus Arthrobotrys oligospora was transformed to hygromycin resistance using the hygromycin-B phosphotransferase gene from Escherichia coli under the control of various heterologous fungal promoters. Plasmid DNA was introduced into fungal protoplasts by polyethylene glycol/CaCl2 treatment. Transformation frequencies varied between 1-6 transformants per microgram DNA. Seven out of 13 integration events analyzed from transformants were single copy integrations, whereas the remaining were multiple and more complex integrations. The addition of restriction enzymes during transformations increased the frequency of single copy integrations. Co-transformation, using the E. coli uidA gene encoding the beta-glucuronidase reporter gene under the control of an Aspergillus nidulans promoter, occurred at frequencies of up to 63%.
Asunto(s)
Hongos Mitospóricos/genética , Nematodos/microbiología , Transformación Genética , Animales , Antibacterianos/farmacología , Enzimas de Restricción del ADN , Farmacorresistencia Microbiana/genética , Genes Fúngicos , Genes Reporteros , Vectores Genéticos , Glucuronidasa , Higromicina B/farmacología , Mitosis , Hongos Mitospóricos/efectos de los fármacosRESUMEN
In previous work, UEA1 and UCSC1, two geographically distinct, powdery mildew isolates, were recognized for their ability to infect Arabidopsis thaliana. We have clarified the identity of these isolates by determining their host ranges, reexamining their morphology, and comparing their DNA sequences for the 5.8S ribosomal RNA and two flanking internal transcribed spacer sequences. These experiments confirm that UEA1 is a member of Erysiphe cruciferarum and that UCSC1 belongs to E. cichoracearum. Interactions of the two Erysiphe isolates with 360 A. thaliana accessions were examined to provide a comprehensive profile of naturally occurring powdery mildew resistance in this weedy species. The majority of A. thaliana accessions (213) were susceptible to both isolates. Among the accessions exhibiting some degree of resistance, most (84) responded differentially to UEA1 and UCSC1 and the remainder were resistant to both isolates. Notably, resistance to UCSC1 cosegregated with RPW7, a locus previously demonstrated to confer resistance to UEA1 in Ms-0 x Landsberg (erecta) crosses. With this large collection of resistant accessions, questions about species specificity, genetic diversity and the evolution of resistance to powdery mildews can be addressed.
Asunto(s)
Arabidopsis/microbiología , Ascomicetos/patogenicidad , Ascomicetos/clasificación , Ascomicetos/genética , Secuencia de Bases , ADN de Hongos , ADN Ribosómico/genética , Microscopía Electrónica de Rastreo , Datos de Secuencia Molecular , ARN Ribosómico/genética , Homología de Secuencia de Ácido NucleicoRESUMEN
The anti-fungal, steroidal, glycoalkaloid saponin, alpha-tomatine, is present in uninfected tomato plants in substantial concentrations, and may contribute to the protection of tomato plants against attack by phytopathogenic fungi. In general, successful fungal pathogens of tomato are more resistant to alpha-tomatine in vitro than fungi that do not infect this plant. For a number of tomato pathogens, this resistance has been associated with the ability to detoxify alpha-tomatine through the action of enzymes known as tomatinases. In contrast, the biotrophic tomato pathogen Cladosporium fulvum is sensitive to alpha-tomatine and is unable to detoxify this saponin. This paper describes the effects of heterologous expression of the cDNA encoding tomatinase from the necrotroph Septoria lycopersici in two different physiological races of C. fulvum. Tomatinase-producing C. fulvum transformants showed increased sporulation on cotyledons of susceptible tomato lines. They also caused more extensive infection of seedlings of resistant tomato lines. Thus, alpha-tomatine may contribute to the ability of tomato to restrict the growth of C. fulvum in both compatible and incompatible interactions.
Asunto(s)
Cladosporium/fisiología , Glicósido Hidrolasas/biosíntesis , Hongos Mitospóricos/fisiología , Solanum lycopersicum/microbiología , Solanum lycopersicum/fisiología , Tomatina/metabolismo , Tomatina/farmacología , Antifúngicos/farmacología , Cladosporium/efectos de los fármacos , Cladosporium/patogenicidad , Cotiledón , Hongos Mitospóricos/efectos de los fármacos , Esporas FúngicasRESUMEN
The pathogenicity of fungal pathogens is presumably dependent on genes that are expressed during infection. In order to isolate such genes from the tomato pathogen Cladosporium fulvum, and to test the hypothesis that starvation-induced genes are also plant induced, a cDNA library was prepared from mycelia grown in a defined medium and then transferred to a starvation medium. The library was then screened with cDNA prepared from starved and replete fungal mycelium. Five unique, differentially expressed cDNAs were isolated from 1,000 clones screened. Northern (RNA) hybridization confirmed that all five were starvation induced. Interestingly, all five were also found to be plant induced. The identity of two of the clones was indicated by partial DNA sequencing as alcohol and aldehyde dehydrogenase. The observed correlation between starvation induction and plant induction in discussed.
Asunto(s)
Cladosporium/genética , Regulación Fúngica de la Expresión Génica , Genes Fúngicos , Solanum lycopersicum/microbiología , Secuencia de Aminoácidos , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Homología de Secuencia de AminoácidoRESUMEN
The extent and causes of yam (Dioscorea rotundata) tuber rots were investigated in Igalaland, Nigeria. Rots were found to be associated with prior physical damage in almost all cases. Numerous fungal species were isolated from the infected lesions of which Fusarium spp. predominated, not Botrydiplodia theobromae as previously reported in other studies. None of the fungi was able to infect undamaged yams in laboratory experiments. The damage was caused by both biotic and abiotic factors of which the yam beetle (Heteroligus meles) was the largest single cause. There were significant differences in the frequency with which different varieties of yam were attacked by H. meles and found to be infected with Fusarium spp. In an independent survey of farmers' experience of yam rots, varieties that scored well correlated with apparent resistance to the beetle and Fusarium spp. These studies highlight the importance of yam beetle infestation and the need to have independent methods for varietal authentication.
Asunto(s)
Escarabajos , Fusarium/aislamiento & purificación , Liliaceae/microbiología , Animales , Manipulación de Alimentos , NigeriaRESUMEN
Arabidopsis thaliana accession La-er was susceptible, and accession Ms-0 was resistant, to powdery mildew diseases caused by Erysiphe cruciferarum UEA1 and E. cichoracearum UCSC1. The resistance reaction phenotype of A. thaliana Ms-0 to both pathogens was characterized, and the resistance loci were genetically mapped. Growth of E. cruciferarum UEA1 on Ms-0 leaves was arrested after formation of the first appressorium: the underlying host epidermal cell collapsed, and occasionally there was necrosis of one or two host mesophyll cells. Growth of E. cichoracearum UCSC1 on Ms-0 leaves was arrested after emergence of several germ tubes from the conidium, and there was necrosis of host mesophyll cells at the sites of infection. Examination of F2 progeny of a cross La-er x Ms-0 indicated that two independently-segregating dominant loci were required for resistance to E. cruciferarum UEA1. One locus, named RPW6, was genetically mapped to chromosome 5, in a 5.6 cM interval flanked by pCITf16 and PI. The other locus, named RPW7, mapped to chromosome 3 in a 8.5 cM interval flanked by CDC2A and AFC1. Independent effects of RPW6 and RPW7 on E. cruciferarum UEA1 could be detected by quantitative measurements of growth of mycelium and production of conidia. Resistance to E. cichoracearum UCSC1 mapped to a single locus, named RPW8, at a location on chromosome 3 which we could not distinguish from RPW7. Evidently, RPW7 and RPW8 define either a complex resistance locus, or a common resistance gene with dual specificity.
Asunto(s)
Arabidopsis/genética , Ascomicetos/patogenicidad , Enfermedades de las Plantas/genética , Arabidopsis/inmunología , Mapeo Cromosómico , Genes de Plantas , Genotipo , Inmunidad Innata/genéticaRESUMEN
We surveyed the use of implantable cardioverter-defibrillators in patients with congenital long QT syndrome. The implantable cardioverter-defibrillator was used primarily in high-risk persons and appeared safe and effective over a mean 31-month follow-up.