RESUMEN
Effects of bovine mastitis pathogen virulence factors on mammary epithelial cell function are not clearly understood. In this study, the effect of streptococcal lipoteichoic acid (LTA), streptokinase, and Escherichia coli lipopolysaccharide (LPS) on proliferation of a primary bovine mammary epithelial cell culture (BTE) and on an established bovine mammary epithelial cell line (MAC-T) was evaluated. Mammary epithelial cells were cultured in the presence of bacterial virulence factors for 48 h at 37 degrees C. BTE cell proliferation was inhibited by streptococcal LTA at 8 and 16 micrograms/ml whereas MAC-T cell proliferation was reduced significantly by concentrations of LTA > or = 2 micrograms/ml. Streptokinase had no effect on proliferation of either MAC-T or BTE cells and LPS inhibited proliferation of BTE but not of MAC-T cells. Effect of LTA and LPS on mammary epithelial cell proliferation could be relevant during the periparturient period when mammary glands are markedly susceptible to new intramammary infection and when mammary epithelial cells undergo extensive proliferation, differentiation and synthesis of milk components.
Asunto(s)
Células Epiteliales/efectos de los fármacos , Escherichia coli/patogenicidad , Lipopolisacáridos/toxicidad , Glándulas Mamarias Animales/citología , Mastitis Bovina/microbiología , Streptococcus/patogenicidad , Estreptoquinasa/farmacología , Ácidos Teicoicos/toxicidad , Animales , Biomarcadores , Bovinos , División Celular/efectos de los fármacos , Línea Celular Transformada , Células Cultivadas , Células Clonales , Infecciones por Escherichia coli/microbiología , Infecciones por Escherichia coli/veterinaria , Femenino , Técnicas para Inmunoenzimas , Queratinas/análisis , Lipopolisacáridos/farmacología , Embarazo , Infecciones Estreptocócicas/microbiología , Infecciones Estreptocócicas/veterinaria , Ácidos Teicoicos/farmacología , Vimentina/análisis , VirulenciaRESUMEN
Effects of bovine mastitis pathogen virulence factors on mammary epithelial cell function are not clearly understood. In this study, the effect of streptococcal lipoteichoic acid (LTA), streptokinase, and Escherichia coli lipopolysaccharide (LPS) on proliferation of a primary bovine mammary epithelial cell culture (BTE) and on an established bovine mammary epithelial cell line (MAC-T) was evaluated. Mammary epithelial cells were cultured in the presence of bacterial virulence factors for 48 h at 37 degrees C. BTE cell proliferation was inhibited by streptococcal LTA at 8 and 16 micrograms/ml whereas MAC-T cell proliferation was reduced significantly by concentrations of LTA > or = 2 micrograms/ml. Streptokinase had no effect on proliferation of either MAC-T or BTE cells and LPS inhibited proliferation of BTE but not of MAC-T cells. Effect of LTA and LPS on mammary epithelial cell proliferation could be relevant during the periparturient period when mammary glands are markedly susceptible to new intramammary infection and when mammary epithelial cells undergo extensive proliferation, differentiation and synthesis of milk components.
Asunto(s)
Animales , Bovinos , Femenino , Embarazo , Ácidos Teicoicos/toxicidad , Células Epiteliales/efectos de los fármacos , Escherichia coli , Lipopolisacáridos/toxicidad , Glándulas Mamarias Animales , Mastitis Bovina , Streptococcus , Estreptoquinasa , Ácidos Teicoicos/farmacología , Línea Celular Transformada , Células Cultivadas , Células Clonales , División Celular/efectos de los fármacos , Técnicas para Inmunoenzimas , Infecciones Estreptocócicas/microbiología , Infecciones Estreptocócicas/veterinaria , Infecciones por Escherichia coli/microbiología , Infecciones por Escherichia coli/veterinaria , Queratinas , Lipopolisacáridos/farmacología , Biomarcadores , Vimentina , VirulenciaRESUMEN
Effects of bovine mastitis pathogen virulence factors on mammary epithelial cell function are not clearly understood. In this study, the effect of streptococcal lipoteichoic acid (LTA), streptokinase, and Escherichia coli lipopolysaccharide (LPS) on proliferation of a primary bovine mammary epithelial cell culture (BTE) and on an established bovine mammary epithelial cell line (MAC-T) was evaluated. Mammary epithelial cells were cultured in the presence of bacterial virulence factors for 48 h at 37 degrees C. BTE cell proliferation was inhibited by streptococcal LTA at 8 and 16 micrograms/ml whereas MAC-T cell proliferation was reduced significantly by concentrations of LTA > or = 2 micrograms/ml. Streptokinase had no effect on proliferation of either MAC-T or BTE cells and LPS inhibited proliferation of BTE but not of MAC-T cells. Effect of LTA and LPS on mammary epithelial cell proliferation could be relevant during the periparturient period when mammary glands are markedly susceptible to new intramammary infection and when mammary epithelial cells undergo extensive proliferation, differentiation and synthesis of milk components.(AU)
Asunto(s)
Animales , Bovinos , Estudio Comparativo , Femenino , Embarazo , RESEARCH SUPPORT, NON-U.S. GOVT , Células Epiteliales/efectos de los fármacos , Escherichia coli/patogenicidad , Lipopolisacáridos/toxicidad , Glándulas Mamarias Animales/citología , Mastitis Bovina/microbiología , Streptococcus/patogenicidad , Estreptoquinasa/farmacología , Ácidos Teicoicos/toxicidad , Biomarcadores , División Celular/efectos de los fármacos , Línea Celular Transformada , Células Cultivadas , Células Clonales , Infecciones por Escherichia coli/microbiología , Infecciones por Escherichia coli/veterinaria , Técnicas para Inmunoenzimas , /análisis , Lipopolisacáridos/farmacología , Infecciones Estreptocócicas/microbiología , Infecciones Estreptocócicas/veterinaria , Ácidos Teicoicos/farmacología , Vimentina/análisis , VirulenciaRESUMEN
Effects of bovine mastitis pathogen virulence factors on mammary epithelial cell function are not clearly understood. In this study, the effect of streptococcal lipoteichoic acid (LTA), streptokinase, and Escherichia coli lipopolysaccharide (LPS) on proliferation of a primary bovine mammary epithelial cell culture (BTE) and on an established bovine mammary epithelial cell line (MAC-T) was evaluated. Mammary epithelial cells were cultured in the presence of bacterial virulence factors for 48 h at 37 degrees C. BTE cell proliferation was inhibited by streptococcal LTA at 8 and 16 micrograms/ml whereas MAC-T cell proliferation was reduced significantly by concentrations of LTA > or = 2 micrograms/ml. Streptokinase had no effect on proliferation of either MAC-T or BTE cells and LPS inhibited proliferation of BTE but not of MAC-T cells. Effect of LTA and LPS on mammary epithelial cell proliferation could be relevant during the periparturient period when mammary glands are markedly susceptible to new intramammary infection and when mammary epithelial cells undergo extensive proliferation, differentiation and synthesis of milk components.