Identification of 5-hydroxytryptamine-producing cells by detection of fluorescence in paraffin-embedded tissue sections.

5-Hydroxytryptamine (5-HT) produced by enterochromaffin (EC) cells is an important enteric mucosal signaling ligand and has been implicated in several gastrointestinal diseases, including inflammatory bowel disease and functional disorders such as irritable bowel syndrome. The present study reports a new, simple and rapid visualization method of 5-HT-producing EC cells utilizing detection of autofluorescence in paraffin-embedded tissue sections after formalin fixation. In human samples, there was a high incidence of autofluorescence+ cells in the 5-HT+ cells in the pyloric, small intestinal and colonic glands, while co-localization was lacking between autofluorescence+ and gastrin+ cells in the pyloric and small intestinal glands. Autofluorescence+ EC cells were detected in the colon of mice and rats. Autofluorescence+ cells were also observed in 5-HT+ ß cells in the pancreatic islets of Langerhans in pregnant mice, while non-pregnant mouse pancreatic islet cells showed no 5-HT immunoreactivity or autofluorescence. These results suggest that autofluorescence+ cells are identical to 5-HT+ cells, and the source of autofluorescence may be 5-HT itself or molecules related to its synthesis or degradation. This autofluorescence signal detection method may be applicable for monitoring of inflammatory status of inflammatory bowel diseases in both the experimental and clinical settings.

Pancreatic carcinosarcoma in a cat.

A 10-year-old female American shorthair cat was presented for evaluation of weight loss. An intra-abdominal mass was found on ultrasonography and laparotomy was performed. The mass was located in the left uterine horn and further masses were found in the pancreas, greater omentum and diaphragm. Microscopical examination revealed that the pancreatic mass had epithelial and mesenchymal components, which on immunohistochemistry expressed cytokeratin and vimentin, respectively. In addition, some spindle cells expressed vimentin and E-cadherin, which might suggest epithelial to mesenchymal transition. In contrast, the uterine, omental and diaphragmatic masses had only mesenchymal composition. The pancreatic lesion is proposed to be a primary carcinosarcoma with metastasis of only the mesenchymal component to distant sites. This the first report of pancreatic carcinosarcoma in a cat.

Total knee arthroplasty in a patient with rheumatoid arthritis and spontaneous fusion of the right hip.

Abstract Bilateral total knee arthroplasty for a painful stiff knee was performed in a 50-year-old woman with rheumatoid arthritis who had spontaneous fusion of the right hip. Despite several manipulations, the range of motion of the right knee worsened. After total hip arthroplasty and ipsilateral knee revision, the right knee had a stable range motion of -15° to 75°. This case suggests that even if the ankylosed hip is in a good position, ipsilateral knee arthroplasty with a fused hip may result in a poorer range of motion than that after total hip arthroplasty.

A new approach to extending the dynamic range in inductively coupled plasma-mass spectrometry.

By the use of a new simultaneous dual stage discrete detector for inductively coupled plasma-mass spectrometry (ICP-MS), a linear dynamic range of 8 orders of magnitude was achieved in a single spectral scan. The design of the detection system and its evaluation are described. The acquisition of the full spectrum of a human urine sample in a single scan is shown.