Alleviation role of exogenous cadaverine on cell cycle, endogenous polyamines amounts and biochemical enzyme changes in barley seedlings under drought stress.

Cadaverine (Cad), which has an independent synthesis pathway compared to other polyamine (PA) types, contributes to the health of plants by regulating plant growth and development, abiotic stress tolerance and antioxidant defense mechanisms. In this work, experiments were carried out to understand the effects of exogenous Cad (10 µM) application under drought stress (%22 PEG 6000) and without stress on cell cycle, total protein content, endogenous PA levels, and biochemical enzyme activities in barley (Hordeum vulgare cv. Burakbey) considering the potential of Cad to stimulate the drought-related tolerance system. Cad application in a stress-free environment showed an effect almost like low-impact drought stress, causing changes in all parameters examined compared to samples grown in distilled water environment (Control). The results clearly show that Cad applied against the negative effects of drought stress on all parameters creates a drought resistance mechanism of the plant. Accordingly, Cad applied together with drought stress increased the density of cells in the cell cycle (G1-S and S-G2 phases) and the amount of endogenous (spermidine 10% and spermine 40%) PAs. In addition, while superoxide dismutase (SOD) (5%), (CAT) (55%) and ascorbate peroxidase (APX) (18%) enzyme levels increased, a stress response mechanism occurred due to the decrease in total protein content (20%) and malondialdehyde (MDA) (80%). As a result, exogenous application of 10 µM Cad showed that it reduced the negative effects of drought stress on endogenous PA amounts, cell division and biochemical activities in barley.

Exogenous L-Carnitine Promotes Plant Growth and Cell Division by Mitigating Genotoxic Damage of Salt Stress.

L-carnitine is a fundamental ammonium compound responsible for energy metabolism in all living organisms. It is an oxidative stress regulator, especially in bacteria and yeast and lipid metabolism in plants. Besides its metabolic functions, l-carnitine has detoxification and antioxidant roles in the cells. Due to the complex interrelationship of l-carnitine between lipid metabolism and salinity dependent oxidative stress, this study investigates the exogenous l-carnitine (1 mM) function on seed germination, cell division and chromosome behaviour in barley seeds (Hordeum vulgare L. cv. Bulbul-89) under different salt stress concentrations (0, 0.25, 0.30 and 0.35 M). The present work showed that l-carnitine pretreatment could not be successful to stimulate cell division on barley seeds under non-stressed conditions compared to stressed conditions. Depending on increasing salinity without pretreatment with l-carnitine, the mitotic index significantly decreased in barley seeds. Pretreatment of barley seeds with l-carnitine under salt stress conditions was found promising as a plant growth promoter and stimulator of mitosis. In addition, pretreatment of barley seeds with l-carnitine alleviated detrimental effects of salt stress on chromosome structure and it protected cells from the genotoxic effects of salt. This may be caused by the antioxidant and protective action of the l-carnitine. Consequently, this study demonstrated that the exogenous application of 1 mM l-carnitine mitigates the harmful effects of salt stress by increasing mitosis and decreasing DNA damage caused by oxidative stress on barley seedlings.

Assessment of shared alleles in drought-associated candidate genes among southern California white oak species (Quercus sect. Quercus).

BACKGROUND: Hybridization and introgression are common phenomena among oak species. These processes can be beneficial by introducing favorable genetic variants across species (adaptive introgression). Given that drought is an important stress, impacting physiological and morphological variation and limiting distributions, our goal was to identify drought-related genes that might exhibit patterns of introgression influenced by natural selection. Using RNAseq, we sequenced whole transcriptomes of 24 individuals from three oaks in southern California: (Quercus engelmannii, Quercus berberidifolia, Quercus cornelius-mulleri) and identified genetic variants to estimate admixture rates of all variants and those in drought genes. RESULTS: We found 398,042 variants across all loci and 4352 variants in 139 drought candidate genes. STRUCTURE analysis of all variants revealed the majority of our samples were assignable to a single species, but with several highly admixed individuals. When using drought-associated variants, the same individuals exhibited less admixture and their allele frequencies were more polarized between Engelmann and scrub oaks than when using the total gene set. These findings are consistent with the hypothesis that selection may act differently on functional genes, such as drought-associated genes, and point to candidate genes that are suggestive of divergent selection among species maintaining adaptive differences. For example, the drought genes that showed the strongest bias against engelmannii-fixed oak variants in scrub oaks were related to sugar transporter, coumarate-coA ligases, glutathione S-conjugation, and stress response. CONCLUSION: This pilot study illustrates that whole transcriptomes of individuals will provide useful data for identifying functional genes that contribute to adaptive divergence among hybridizing species.