Histaminergic System and Inflammation-Related Genes in Normal Large Intestine and Adenocarcinoma Tissues: Transcriptional Profiles and Relations.

Transcriptional analyses such as microarray data have contributed to the progress in the diagnostics and therapy of colorectal cancer (CRC). The need for such research is still present because of the disease being common in both men and women with a high second position in cancer rankings. Little is known about the relations between the histaminergic system and inflammation in the large intestine and CRC. Therefore, the aim of this study was to evaluate the expression of genes related to the histaminergic system and inflammation in the CRC tissues at three cancer development designs: all tested CRC samples, low (LCS) and high (HCS) clinical stage, and four clinical stages (CSI-CSIV), to the control. The research was carried out at the transcriptomic level, analysing hundreds of mRNAs from microarrays, as well as carrying out RT-PCR analysis of histaminergic receptors. The following histaminergic mRNAs: GNA15, MAOA, WASF2A, and inflammation-related: AEBP1, CXCL1, CXCL2, CXCL3, CXCL8, SPHK1, TNFAIP6, were distinguished. Among all analysed transcripts, AEBP1 can be considered the most promising diagnostic marker in the early stage of CRC. The results showed 59 correlations between differentiating genes of the histaminergic system and inflammation in the control, control and CRC, and CRC. The tests confirmed the presence of all histamine receptor transcripts in both the control and colorectal adenocarcinoma. Significant differences in expression were stated for HRH2 and HRH3 in the advanced stages of CRC adenocarcinoma. The relations between the histaminergic system and inflammation-linked genes in both the control and the CRC have been observed.

Expression Level of Genes Coding for Cell Adhesion Molecules of Cadherin Group in Colorectal Cancer Patients.

BACKGROUND: Colorectal Cancer (CRC) is one of the most frequently diagnosed neoplasms and also one of the main death causes. Cell adhesion molecules are taking part in specific junctions, contributing to tissue integrality. Lower expression of the cadherins may be correlated with poorer differentiation of the CRC, and its more aggressive phenotype. The aim of the study is to designate the cadherin genes potentially useful for the diagnostics, prognostics, and the treatment of CRC. MATERIAL AND METHODS: Specimens were collected from 28 persons (14 female and 14 male), who were operated for CRC. The molecular analysis was performed using oligonucleotide microarrays, mRNA used was collected from adenocarcinoma, and macroscopically healthy tissue. The results were validated using qRT-PCR technique. RESULTS: Agglomerative hierarchical clustering of normalized mRNA levels has shown 4 groups with statistically different gene expression. The control group was divided into 2 groups, the one was appropriate control (C1), the second (C2) had the genetic properties of the CRC, without pathological changes histologically and macroscopically. The other 2 groups were: LSC (Low stage cancer) and HSC (High stage cancer). Consolidated results of the fluorescency of all of the differential genes, designated two coding E-cadherin (CDH1) with the lower expression, and P-cadherin (CDH3) with higher expression in CRC tissue. CONCLUSIONS: The levels of genes expression are different for several groups of cadherins, and are related with the stage of CRC, therefore could be potentially the useful marker of the stage of the disease, also applicable in treatment and diagnostics of CRC.

[The application of transversostomy in colorectal cancer surgery]. / Rola odbarczajacej transwersostomii w chirurgii raka jelita grubego.

UNLABELLED: Defunctioning stomas (ileostomy and transversostomy) are the most common methods of the decompression of distal digestive tract. The aim of this study was to assess the results of transverse loop colostomy in colorectal cancer patients. MATERIAL AND METHOD: Between 1998 and 2007, 119 patients with colorectal cancer underwent transversostomy. In 97 patients the colostomy was formed during the primary operation as a planned prevention from further complications (1st group) and in 22 previously operated patients due to postoperative complications as a second procedure (2nd group). RESULTS: In the study group, there were 2 deaths in the perioperative period due to the anastomosis leakage and the intraabdominal sepsis. Three patients required reconstructive operation because of the colostomy stenosis and further 3 due to colostomy prolapse. There were also several cases of superficial wound infection after termination of transversostomy. Almost 78 patients in total had the restoration of digestive tract continuity performed, prevalently from the 1st group. CONCLUSIONS: 1. In our opinion transversostomy is an effective solution for the proximal faecal diversion especially at high risk of anastomosis insufficiency or with present anastomosis leak. 2. In our study group, the incidence of complications (such as: chyme loss or dyselectrolytemia) in patients with performed transversostomy was low, thus we find it recommendable for the colon decompression.

[Cecum mobility as a cause of ileus]. / Ruchoma katnica przyczyna niedroznosci przewodu pokarmowego.

The authors present a rare case of ileus caused by unusual cecum mobility. The 31-years old female patient was diagnosed ineffectively several times due to abdominal pain. The diagnostics, an attempt of a conservative treatment, along with surgical and postoperative treatment are discussed.