Enhanced Stability of Dopamine Delivery via Hydrogel with Integrated Graphene.

The synthesis of graphene-based materials for drug delivery represents an area of active research, and the use of graphene in drug delivery systems is promising due to its unique properties. Thus, in the present work, we discuss the potential of few-layer graphene in a hydrogel system for dopamine release. The hydrogels are frequently used for these systems for their special physico-chemical properties, which can ensure that the drug is effectively released in time. However, the release from such structures is mostly determined by diffusion alone, and to overcome this restriction, the hydrogel can be "improved" with nanoscale fillers like graphene. The release kinetics of the composite obtained were analyzed to better understand how the use of graphene, instead of the more common graphene oxide (GO) and reduced graphene oxide (rGO), affects the characteristics of the system. Thus, the systems developed in this study consist of three main components: biopolymer, graphene, and dopamine. The hydrogels with graphene were prepared by combining two different solutions, one with polyacrylic acid and agarose and one with graphene prepared by the exfoliation method with microwave irradiation. The drug delivery systems were developed by adding dopamine to the obtained hydrogels. After 24 h of release, the presence of dopamine was observed, demonstrating that the system developed can slow down the drug's degradation because of the interactions with the graphene nanoplates and the polymer matrix.

Correlation of Elemental Transfer, Bioactive Compounds and Antioxidant Activity on Lactuca sativa L. Grown in Soil with Functionalized CNT and HMs.

While heavy metals (HM) have been considered in recent decades to be the most common and problematic pollutants, the expansion of the list of pollutants due to the active use of carbon nanotubes (CNT) raises new questions about the benefit and harm of HM released to nature individually or fixed on CNT walls. A pot experiment was conducted to compare the effect of two classes of potential pollutants-metal salts of Pb, Mn, Cu, Zn, Cd, and Ni; and functionalized CNTs with COOH, MnO2, Fe3O4, and MnO2-Fe3O4-applied in soil, on the elemental transfer, the bioactive compounds accumulation, and the antioxidant activity in lettuce. While CNTs mainly increased the elemental transfer from soil to leaves, HM salts strongly obstructed it. In the presence of CNTs, the antioxidant activity in lettuce leaves correlated with the transfer of elements from soil to root and from root to leaves. The excess of HMs in soil induced a greater variation of the polyphenols quantity and antioxidant activity than the excess of CNTs. It might be assumed that lettuce perceived HMs as a more aggressive stressor than CNTs and more strongly activated the defense mechanism, showing the reduction of the element transfer and enhancing of total polyphenol production and antioxidant activity.

The Antibacterial Properties of Nanocomposites Based on Carbon Nanotubes and Metal Oxides Functionalized with Azithromycin and Ciprofloxacin.

Different microorganisms are present in nature, some of which are assumed to be hazardous to the human body. It is crucial to control their continuing growth to improve human life. Nanomaterial surface functionalization represents a current topic in continuous evolution that supports the development of new materials with multiple applications in biology, medicine, and the environment. This study focused on the antibacterial activity of different nanocomposites based on functionalized multi-walled carbon nanotubes against four common bacterial strains. Two metal oxides (CuO and NiO) and two antibiotics (azithromycin and ciprofloxacin) were selected for the present study to obtain the following nanocomposites: MWCNT-COOH/Antibiotic, MWCNT-COOH/Fe3O4/Antibiotic, and MWCNT-COOH/Fe3O4/MO/Antibiotic. The present study included two Gram-positive bacteria (Staphylococcus aureus and Enterococcus faecalis) and two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Ciprofloxacin (Cip) functionalized materials (MWCNT-COOH/Fe3O4/Cip) were most efficient against all tested bacterial strains; therefore, we conclude that Cu and Ni reduce the effects of Cip. The obtained results indicate that the nanocomposites functionalized with Cip are more effective against selected bacteria strains compared to azithromycin (Azi) functionalized nanocomposites. The current work determined the antibacterial activities of different nanocomposites and gave fresh insights into their manufacture for future research regarding environmental depollution.

The Effect of Copper Salts on Bioactive Compounds and Ultrastructure of Wheat Plants.

Abiotic stress agents, among them metal stress, can cause oxidative damage to plant cells. In defense, plants can increase the production of secondary metabolites in order to mitigate the harmful effects caused by them. The purpose of this work was to evaluate the effect of two types of copper salts (CuSO4 and Cu(NO3)2), added in two different amounts in soil (150 mg/kg, respectively 300 mg/kg), on assimilating pigments, total polyphenols, antioxidant activity and the elemental composition of wheat. The obtained results were compared with those from control plants grown in the same conditions but without copper salts. The amount of assimilating pigments, total polyphenols, and antioxidant activity respectively increases or decreases in the plants treated with copper salts compared to the control depending on the stage of development of the plant. No significant damage induced in the leaves of the wheat plants treated with the selected salts was observed following the TEM analysis. In six-week-old plants it was observed by EDX analysis that the salts are transformed into nanoparticles. The bioactive compounds, elemental composition and their interaction is influenced by concentration of metal's salt, type of salt and exposure period.

The Effect of TiO2 Nanoparticles on the Composition and Ultrastructure of Wheat.

The present work aims to follow the influence of TiO2 nanoparticles (TiO2 NPs) on bioactive compounds, the elemental content of wheat, and on wheat leaves' ultrastructure. Synthesized nanoparticles were characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and transmission electron microscopy (TEM). The concentration of phenolic compounds, assimilation pigments, antioxidant capacity, elemental content, as well as the ultrastructural changes that may occur in the wheat plants grown in the presence or absence of TiO2 NPs were evaluated. In plants grown in the presence of TiO2 NPs, the amount of assimilating pigments and total polyphenols decreased compared to the control sample, while the antioxidant activity of plants grown in amended soil was higher than those grown in control soil. Following ultrastructural analysis, no significant changes were observed in the leaves of TiO2-treated plants. Application of TiO2 NPs to soil caused a significant reaction of the plant to stress conditions. This was revealed by the increase of antioxidant capacity and the decrease of chlorophyll, total polyphenols, and carotenoids. Besides, the application of TiO2 NPs led to significant positive (K, Zn, Br, and Mo) and negative (Na, Mn, Fe, As, Sr, Sb, and Ba) variation of content.

Investigating the effects of non-steroidal anti-inflammatory drugs (NSAIDs) on the composition and ultrastructure of green leafy vegetables with important nutritional values.

The global presence of pharmaceuticals in the environment has been particularly considered a concerning problem with unknown consequences. Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most frequently prescribed drugs in the world, and as a result, they are commonly found in different environmental compartments. In the present work, we studied the effects of NSAIDs (diclofenac, ibuprofen, and naproxen) on the composition and ultrastructure of Atriplex patula L., S. oleracea, and Lactuca sativa L., three green leafy vegetables with significant nutritional value. Contaminant solutions of NSAIDs were applied every two days using concentrations of 0.1 mg L-1, 0.5 mg L-1, and 1 mg L-1. After eight weeks of exposure of the green leafy vegetables to the selected NSAIDs, the chlorophylls (a + b), carotenoids (zeaxanthin, lutein, and ß-carotene), total polyphenol and total flavonoid contents, antioxidant capacity, and the ultrastructural modifications were determined. The obtained results indicated a moderate reduction in the assimilating pigments, total polyphenol and flavonoid contents. In addition, ultrastructural damages of the chloroplasts and cell walls were observed in the leaves of the selected vegetables, which were exposed to abiotic stress-induced by NSAIDs. All data collectively suggest that this group of drugs induced harmful effects on plants, and implicitly they may also negatively affected human health on the long term.