Light emitting diode effect of red, blue, and amber light on photosynthesis and plant growth parameters.

The visible light spectrum (400-700 nm) powers plant photosynthesis and innumerable other biological processes. Photosynthesis curves plotted by pioneering photobiologists show that amber light (590-620 nm) induces the highest photosynthetic rates in this spectrum. Yet, both red and blue light are viewed superior in their influence over plant growth. Here we report two approaches for quantifying how light wavelength photosynthesis and plant growth using light emitting diodes (LEDs). Resolved quantum yield spectra of tomato and lettuce plants resemble those acquired earlier, showing high quantum utilization efficiencies in the 420-430 nm and 590-620 nm regions. Tomato plants grown under blue (445 nm), amber (595 nm), red (635 nm), and combined red-blue-amber light for 14 days show that amber light yields higher fresh and dry mass, by at least 20%. Principle component analysis shows that amber light has a more pronounced and direct effect on fresh mass, whereas red light has a major effect on dry mass. These data clarify amber light's primary role in photosynthesis and suggest that bandwidth determines plant growth and productivity under sole amber lighting. Findings set precedence for future work aimed at maximizing plant productivity, with widespread implications for controlled environment agriculture.

Microencapsulation of ultrasound-assisted phenolic extracts of sugar maple leaves: Characterization, in vitro gastrointestinal digestion, and storage stability.

Sugar maple leaves (SML), usually considered residue plant biomass and discarded accordingly, contain a considerable amount of phenolic antioxidants. In this study, SML phenolics were extracted employing both advanced (homogenization pretreated ultrasound-assisted extraction) and conventional (maceration) methods followed by their encapsulation by freeze drying and spray drying using a combination of maltodextrin and gum arabic as coating agents. Detailed physicochemical analyses revealed that the encapsulated microparticles had high solubility (>90 %) and encapsulation efficiency (>95 %), acceptable thermal stability with good handling properties. Phenolic compounds were completely released from microparticles during simulated gastric conditions. The microparticles influenced the bioaccessibility of more than 43 % of the phenolic fraction in the intestinal phase. The antioxidant capacity of the microparticles was preserved during storage. These findings suggest the effectiveness of the microencapsulation process for producing high quality microparticles of SML phenolic extracts and the possibility of their use in the food, nutraceutical, bio-pharmaceutical sectors.

Germicidal efficacy of continuous and pulsed ultraviolet-C radiation on pathogen models and SARS-CoV-2.

Ultraviolet radiation's germicidal efficacy depends on several parameters, including wavelength, radiant exposure, microbial physiology, biological matrices, and surfaces. In this work, several ultraviolet radiation sources (a low-pressure mercury lamp, a KrCl excimer, and four UV LEDs) emitting continuous or pulsed irradiation were compared. The greatest log reductions in E. coli cells and B. subtilis endospores were 4.1 ± 0.2 (18 mJ cm-2) and 4.5 ± 0.1 (42 mJ cm-2) with continuous 222 nm, respectively. The highest MS2 log reduction observed was 2.7 ± 0.1 (277 nm at 3809 mJ cm-2). Log reductions of SARS-CoV-2 with continuous 222 nm and 277 nm were ≥ 3.4 ± 0.7, with 13.3 mJ cm-2 and 60 mJ cm-2, respectively. There was no statistical difference between continuous and pulsed irradiation (0.83-16.7% [222 nm and 277 nm] or 0.83-20% [280 nm] duty rates) on E. coli inactivation. Pulsed 260 nm radiation (0.5% duty rate) at 260 nm yielded significantly greater log reduction for both bacteria than continuous 260 nm radiation. There was no statistical difference in SARS-CoV-2 inactivation between continuous and pulsed 222 nm UV-C radiation and pulsed 277 nm radiation demonstrated greater germicidal efficacy than continuous 277 nm radiation. Greater radiant exposure for all radiation sources was required to inactivate MS2 bacteriophage. Findings demonstrate that pulsed irradiation could be more useful than continuous UV radiation in human-occupied spaces, but threshold limit values should be respected. Pathogen-specific sensitivities, experimental setup, and quantification methods for determining germicidal efficacy remain important factors when optimizing ultraviolet radiation for surface decontamination or other applications.

Phenolic mapping and associated antioxidant activities through the annual growth cycle of sugar maple leaves.

Concentrations of antioxidant components (analyzed by HPLC-UV) and antioxidant attributes (assayed by radical scavenging and non-radical redox potential methods) of sugar maple leaves (SML) from different harvesting times were investigated. Moreover, measurements of colorimetry, SEM, and FTIR spectroscopy-based characterization of leaves composition, throughout the growth cycle, were performed. Results showed that the antioxidant activities of SML are strongly correlated with phenolic contents and significantly (p < 0.05) varied with harvesting time where minimum amount of total phenolics (105.67 ± 13.16 mg GAE/g DM) and total flavonoids (3.27 ± 0.26 mg CTE/g DM) were found to be concentrated in Fall leaves. The absorption bands obtained from FTIR spectra revealed the presence of functional groups that have great significance towards the antioxidant activity of SML. Principal component analysis revealed that biosynthesis of maximum phenolic compounds in SML mostly occurs during the leaf expansion and growth phases. The obtained data provided a better understanding towards the effect of harvesting time on the phenolic mapping of SML in favor of its valorization into functional food ingredients.

Relationship between Total Antioxidant Capacity, Cannabinoids and Terpenoids in Hops and Cannabis.

Efficient determination of antioxidant activity in medicinal plants may provide added value to extracts. The effects of postharvest pre-freezing and drying [microwave-assisted hot air (MAHD) and freeze drying] on hops and cannabis were evaluated to determine the relationship between antioxidant activity and secondary metabolites. The 2,2-diphenyl-1-picrylhydrazine (DPPH) reduction and ferric reducing ability of power (FRAP) assays were assessed for suitability in estimating the antioxidant activity of extracted hops and cannabis inflorescences and correlation with cannabinoid and terpene content. Antioxidant activity in extracts obtained from fresh, undried samples amounted to 3.6 Trolox equivalent antioxidant activity (TEAC) (M) dry matter-1 and 2.32 FRAP (M) dry matter-1 for hops, in addition to 2.29 TEAC (M) dry matter-1 and 0.25 FRAP (M) dry matter-1 for cannabis. Pre-freezing significantly increased antioxidant values by 13% (DPPH) and 29.9% (FRAP) for hops, and by 7.7% (DPPH) and 19.4% (FRAP) for cannabis. ANOVA analyses showed a significant (p < 0.05) increase in total THC (24.2) and THCA (27.2) concentrations (g 100 g dry matter-1) in pre-frozen, undried samples compared to fresh, undried samples. Freeze-drying and MAHD significantly (p < 0.05) reduced antioxidant activity in hops by 79% and 80.2% [DPPH], respectively and 70.1% and 70.4% [FRAP], respectively, when compared to antioxidant activity in extracts obtained from pre-frozen, undried hops. DPPH assay showed that both freeze-drying and MAHD significantly (p < 0.05) reduced the antioxidant activity of cannabis by 60.5% compared to the pre-frozen samples although, there was no significant (p < 0.05) reduction in the antioxidant activity using the FRAP method. Greater THC content was measured in MAHD-samples when compared to fresh, undried (64.7%) and pre-frozen, undried (57%), likely because of decarboxylation. Both drying systems showed a significant loss in total terpene concentration, yet freeze-drying has a higher metabolite retention compared to MAHD. These results may prove useful for future experiments investigating antioxidant activity and added value to cannabis and hops.

Ultrasound-Assisted Extraction of Antioxidants from Melastoma malabathricum Linn.: Modeling and Optimization Using Box-Behnken Design.

This study presents modeling and optimization of ultrasound-assisted extraction (UAE) of Melastoma malabathricum with the objective of evaluating its phytochemical properties. This one-factor-at-a-time (OFAT) procedure was conducted to screen for optimization variables whose domains included extraction temperature (XET), ultrasonic time (XUT), solvent concentration (XSC), and sample-to-liquid ratio (XSLR). Response surface methodology (RSM) coupled with Box-Behnken design (BBD) was applied to establish optimum conditions for maximum antioxidant extraction. Modeling and optimization conditions of UAE at 37 kHz, XET 32 °C for XUT 16 min and dissolved in an XSC 70% ethanol concentration at a XSLR 1:10 ratio yielded scavenging effects on 2,2-diphenyl-1-picryl-hydrazyl (DPPH) at 96% ± 1.48 and recorded values of total phenolic content (TPC) and total flavonoid content (TFC) at 803.456 ± 32.48 mg GAE (gallic acid equivalents)/g, and 102.972 ± 2.51 mg QE (quercetin equivalents)/g, respectively. The presence of high flavonoid compounds was verified using TWIMS-QTOFMS. Chromatic evaluation of phytochemicals using gas chromatography-mass spectrometry (GC-MS) revealed the presence of 14 phytocompounds widely documented to play significant roles in human health. This study provides a comparative evaluation with other studies and may be used for validation of the species' potential for its much-acclaimed medicinal and cosmeceutical uses.

An overview of different homogenizers, their working mechanisms and impact on processing of fruits and vegetables.

Fruits and vegetables (F&V) are the second highest recommended foods, rich in antioxidants, vitamins and minerals, vital for building immunity against chronic diseases. F&V processing involves particle size reduction, for which different types of homogenizers, categorized as mechanical homogenizers, pressure homogenizers and ultrasonic homogenizers are used. The review discusses different types of homogenizers, their working mechanism, and application in F&V processing. Among mechanical homogenizers, knife mills are used for primary size reduction, ball mills for the micronization of dried F&V and rotor-stator homogenizers for emulsification. Use of the ultrasonic homogenizer is limited to extraction of bioactive compounds or as a pre-treatment for dehydration of F&V. High-pressure homogenizers are most widely used and reported due to the synergistic effect of homogenization and temperature increase, resulting in longer shelf-life and better physicochemical properties of the product. Additionally, the review also explains the effect of homogenization on the physicochemical, sensory and nutraceutical properties of the product.

Green extraction and characterization of leaves phenolic compounds: a comprehensive review.

Although containing significant levels of phenolic compounds (PCs), leaves biomass coming from either forest, agriculture, or the processing industry are considered as waste, which upon disposal, brings in environmental issues. As the demand for PCs in functional food, pharmaceutical, nutraceutical and cosmetic sector is escalating day by day, recovering PCs from leaves biomass would solve both the waste disposal problem while ensuring a valuable "societal health" ingredient thus highly contributing to a sustainable food chain from both economic and environmental perspectives. In our search for environmentally benign, efficient, and cost-cutting techniques for the extraction of PCs, green extraction (GE) is presenting itself as the best option in modern industrial processing. This current review aims to highlight the recent progress, constraints, legislative framework, and future directions in GE and characterization of PCs from leaves, concentrating particularly on five plant species (tea, moringa, stevia, sea buckthorn, and pistacia) based on the screened journals that precisely showed improvements in extraction efficiency along with maintaining extract quality. This overview will serve researchers and relevant industries engaged in the development of suitable techniques for the extraction of PCs with increasing yield.

Cold Ethanol Extraction of Cannabinoids and Terpenes from Cannabis Using Response Surface Methodology: Optimization and Comparative Study.

Efficient cannabis biomass extraction can increase yield while reducing costs and minimizing waste. Cold ethanol extraction was evaluated to maximize yield and concentrations of cannabinoids and terpenes at different temperatures. Central composite rotatable design was used to optimize two independent factors: sample-to-solvent ratio (1:2.9 to 1:17.1) and extraction time (5.7 min-34.1 min). With response surface methodology, predicted optimal conditions at different extraction temperatures were a cannabis-to-ethanol ratio of 1:15 and a 10 min extraction time. With these conditions, yields (g 100 g dry matter-1) were 18.2, 19.7, and 18.5 for -20 °C, -40 °C and room temperature, respectively. Compared to the reference ground sample, tetrahydrocannabinolic acid changed from 17.9 (g 100 g dry matter-1) to 15, 17.5, and 18.3 with an extraction efficiency of 83.6%, 97.7%, 102.1% for -20 °C, -40 °C, and room temperature, respectively. Terpene content decreased by 54.1% and 32.2% for extraction at -20 °C and room temperature, respectively, compared to extraction at -40 °C. Principal component analysis showed that principal component 1 and principal component 2 account for 88% and 7.31% of total variance, respectively, although no significant differences in cold ethanol extraction at different temperatures were observed.

Microwave- and Ultrasound-Assisted Extraction of Cannabinoids and Terpenes from Cannabis Using Response Surface Methodology.

Limited studies have explored different extraction techniques that improve cannabis extraction with scale-up potential. Ultrasound-assisted and microwave-assisted extraction were evaluated to maximize the yield and concentration of cannabinoids and terpenes. A central composite rotatable design was used to optimize independent factors (sample-to-solvent ratio, extraction time, extraction temperature, and duty cycle). The optimal conditions for ultrasound- and microwave-assisted extraction were the sample-to-solvent ratios of 1:15 and 1:14.4, respectively, for 30 min at 60 °C. Ultrasound-assisted extraction yielded 14.4% and 14.2% more oil and terpenes, respectively, compared with microwave-assisted extracts. Ultrasound-assisted extraction increased cannabinoid concentration from 13.2−39.2%. Considering reference ground samples, tetrahydrocannabinolic acid increased from 17.9 (g 100 g dry matter−1) to 28.5 and 20 with extraction efficiencies of 159.2% and 111.4% for ultrasound-assisted and microwave-assisted extraction, respectively. Principal component analyses indicate that the first two principal components accounted for 96.6% of the total variance (PC1 = 93.2% and PC2 = 3.4%) for ultrasound-assisted extraction and 92.4% of the total variance (PC1 = 85.4% and PC2 = 7%) for microwave-assisted extraction. Sample-to-solvent ratios significantly (p < 0.05) influenced the secondary metabolite profiles and yields for ultrasound-assisted extracts, but not microwave-assisted extracts.

Semisynthetic transformation of banana peel to enhance the conversion of sugars to 5-hydroxymethylfurfural.

This study aimed to efficiently convert banana peels (BP) into 5-hydroxymethylfurfural (HMF) by using an integrated mechanoenzymatic/catalytic approach. There is no report on HMF production using mechanoenzymatic hydrolysis. Moreover, this method enables saccharification of lignocellulose without bulk solvents or pretreatment. The effects of the reaction volume, milling time, and reactive aging (RAging) on the mechanoenzymatic hydrolysis of BP were studied. The solvent-free enzymatic hydrolysis of BP under RAging conditions was found to provide higher glucose (40.5 wt%) and fructose (17.2 wt%) yields than chemical hydrolysis. Next, the conversion of the resulting monosaccharides into HMF in the presence of the AlCl3·H2O/HCl-DMSO/H2O system resulted in 71.9 mol% yield, which is so far the highest HMF yield obtained from cellulosic food wastes. Under identical reaction conditions, direct conversion of untreated BP to HMF yielded 22.7 mol% HMF, suggesting that mechanoenzymatic hydrolysis greatly promotes the release of sugars from BP to improve HMF yield.

Effect of plasma activated water on Escherichia coli disinfection and quality of kale and spinach.

Plasma activated water (PAW) is one of the promising technologies for fresh food disinfection. In this study, PAW was generated by activating water under nonthermal plasma for 10, 20, 30, 45 and 60 min. The effectiveness of Escherichia coli inactivation by PAW treatment on kale and spinach samples was assessed. The differences between kale and spinach samples in terms of the product quality and nutritional characteristics upon PAW treatment was also investigated. Further, changes in leaf structure and surface morphology upon PAW treatment was also evaluated through FTIR cuticle analysis and SEM imaging of leaf surfaces. Results showed that, around 6 log CFU/g reduction in E. coli population was observed in PAW-45 min treatment. However, PAW treatment significantly reduced the total chlorophyll content in both kale and spinach. The total phenolic content, flavonoid content and ascorbic content were altered according to the PAW activation time. Further, kale and spinach behaved differently in terms of antioxidant activity and membrane electrolytic leakage values upon PAW treatment. Clear changes in the cuticular layer and the surface morphological characteristics of the leaf samples were observed after PAW which could be the reason for the significant differences between kale and spinach characteristics in response to PAW treatments.

Influence of plasma activated water treatment on enzyme activity and quality of fresh-cut apples.

Plasma activated water (PAW) is a new approach to disinfecting surfaces including fresh-cut foods while maintaining their quality attributes. The aim of this study was to evaluate the effect of PAW on enzyme activity, microbial and physicochemical quality of fresh-cut apples. PAW was produced at different production activation times of 10 min, 20 min, 30 min, 45 min and 60 min and the fresh-cut apple slices were washed with PAW for 5 min and stored at 4 °C for 12 days. Results showed that PAW treatments reduced the polyphenol oxidase activity immediately after treatment and the lowest activity was recorded in PAW-20 min (5.10 ± 0.16 U/g FW) after 12 days. Conversely, peroxidase activity of the samples increased immediately after PAW treatment and the samples treated with PAW activated for 30 min had the lowest peroxidase activity at the end of 12 days of storage. No significant changes in the total phenolic content and FRAP antioxidant activity of the fresh-cut apple samples after PAW treatments. The results from firmness, membrane permeability, respiration rate and microstructural imaging showed that at higher PAW activation times (45 min and 60 min) had adverse effects on the quality of fresh-cut apples. Significant reductions in the total aerobic bacteria and total yeast and molds were observed in all PAW treatments except PAW activated for 10 min. The results suggests that plasma activated water could maintain the quality of the fresh-cut apples during storage for plasma activation times of 20 min and 30 min for up to 12 days of storage.

Solid-state co-culture fermentation of simulated food waste with filamentous fungi for production of bio-pigments.

The use of waste stream residues as feedstock for material production simultaneously helps reduce dependence on fossil-based resources and to shift toward a circular economy. This study explores the conversion of food waste into valuable chemicals, namely, bio-pigments. Here, a simulated food waste feedstock was converted into pigments via solid-state fermentation with the filamentous fungus Talaromyces albobiverticillius (NRRL 2120). Pigments including monascorubrin, rubropunctatin, and 7-(2-hydroxyethyl)-monascorubramine were identified as products of the fermentation via ultra-performance liquid chromatography coupled with quadrupole-time-of-flight electrospray ionization mass spectrometry. Pigments were obtained at concentrations of 32.5, 20.9, and 22.4 AU/gram dry substrate for pigments absorbing at 400, 475, and 500 nm, respectively. Pigment production was further enhanced by co-culturing T. albobiverticillius with Trichoderma reesei (NRRL 3652), and ultimately yielded 63.8, 35.6, and 43.6 AU/gds at the same respective wavelengths. This represents the highest reported production of pigments via solid-state fermentation of a non-supplemented waste stream feedstock. KEY POINTS: • Simulated food waste underwent solid-state fermentation via filamentous fungi. • Bio-pigments were obtained from fermentation of the simulated food waste. • Co-culturing multiple fungal species substantially improved pigment production.

Development of a Nuclear Magnetic Resonance Method and a Near Infrared Calibration Model for the Rapid Determination of Lipid Content in the Field Pea (Pisum sativum).

Pisum sativum is a leguminous crop suitable for cultivation worldwide. It is used as a forage or dried seed supplement in animal feed and, more recently, as a potential non-traditional oilseed. This study aimed to develop a low-cost, rapid, and non-destructive method for analyzing pea lipids with no chemical modifications that would prove superior to existing destructive solvent extraction methods. Different pea accession seed samples, prepared as either small portions (0.5 mm2) of endosperm or ground pea seed powder for comparison, were subjected to HR-MAS NMR analyses and whole seed samples underwent NIR analyses. The total lipid content ranged between 0.57-3.45% and 1.3-2.6% with NMR and NIR, respectively. Compared to traditional extraction with butanol, hexane-isopropanol, and petroleum ether, correlation coefficients were 0.77 (R2 = 0.60), 0.56 (R2 = 0.47), and 0.78 (R2 = 0.62), respectively. Correlation coefficients for NMR compared to traditional extraction increased to 0.97 (R2 = 0.99) with appropriate correction factors. PLS regression analyses confirmed the application of this technology for rapid lipid content determination, with trends fitting models often close to an R2 of 0.95. A better robust NIR quantification model can be developed by increasing the number of samples with more diversity.

Techno-Economic Analysis of the Multiple-Pass Ultrasonication with Mechanical Homogenization (MPUMH) Processing of Processed Carrot Discards to Puree.

A sustainable valorization process for puree processing from processed carrot discards (PDCs) was proposed by using multiple-pass ultrasonication with mechanical homogenization (MPUMH), optimized at 9 min ultrasonication followed by mechanical homogenization for 1 min, subjected to three passes. Techno-economic analysis of the puree processing plant was studied for two process models using SuperPro Designer for a plant with a capacity of 17.4 MT/day, operational for 26 weeks, with a 20-year lifetime. The two scenarios were (i) base case (PDCs processed without peels and crowns) and (ii) case 2 (PDCs and carrots (50:50, w/w) processed with peels and crowns). Both scenarios were economically feasible with an internal rate of return (IRR) and return on investment (ROI) at 24.71% and 31.04% (base case) and 86.11% and 119.87% (case 2), respectively. Case 2 had a higher total capital investment (Can$13.7 million) but a lower annual operating cost (Can$8.9 million), resulting in greater revenue generation (Can$29.7 million), thus offering a higher ROI. Sensitivity analysis related to the number of passes on puree quality and price is suggested to lower the capital investment. For the base case, a lower ROI was due to the high labor cost incurred for manual peeling of PDCs, indicating the critical need for developing a commercial peeler equipped to cut labor costs and increase profitability. The study casts insights into the techno-economic performance of a sustainable process for the valorization of PDCs.

Use of filamentous fungi as biocatalysts in the oxidation of 5-(hydroxymethyl)furfural (HMF).

The objective of this study was to explore the use of filamentous fungi as oxidative biocatalysts. To that end, filamentous fungal whole-cells, comprising five different species were employed in the oxidation of 5-(hydroxymethyl)furfural (HMF). Two species (A. niger and T. reesei), which demonstrated superior HMF conversion and product accumulation, were further evaluated for growth on alternative substrates (e.g. pentoses) as well as for use in a chemo-biocatalytic reaction system. Concerning the latter, the two whole-cell biocatalysts were coupled with laccase/TEMPO in a one-pot reaction designed to enable catalysis of the three oxidative steps necessary to convert HMF into 2,5-furandicarboxylic acid (FDCA), a compound with immense potential in the production of sustainable and eco-friendly polymers. Ultimately, the optimal one-pot chemo-biocatalytic cascade system, comprising 1 g/L T. reesei whole cells coupled with 2.5 mM laccase and 20 mol% TEMPO, achieved a molar yield of 88% after 80 h.

Encapsulation of Russian Olive Water Kefir as an Innovative Functional Drink with High Antioxidant Activity.

Processing of Russian olive water kefir (RWK), as a fermented functional drink made with Russian olive juice and water kefir grains with high antioxidant activity, into powder is crucial for improving its stability for the commercialization of this product. For the first time, this study aimed to encapsulate water kefir microorganisms and bioactive compounds in RWK using carrier materials to develop a synbiotic functional powder using spray drying as an encapsulation method. The goal was maximizing antioxidant activity, product yield, and survival rate of water kefir microorganisms in the produced Russian olive water kefir powder. The optimal spray drying conditions were observed to be at an inlet air temperature of 120ºC, 35 % feed flow rate, and 7 % concentration of drying aid. The effects of spray drying conditions on the quality of microcapsules were assessed and modeled, and the validity of the model was verified. Also, the spray-dried powder's physicochemical properties were assessed and showed promising microbial and physicochemical characteristics compared with the freeze-dried powder.

Phycoremediation and valorization of synthetic dairy wastewater using microalgal consortia of Chlorella variabilis and Scenedesmus obliquus.

Microalgae are known to grow on wastewater utilizing their available nutrients. The residual algal biomass thus obtained could be used for producing value-added products thereby making it an economically viable and sustainable option for the dairy industry. The present study evaluates the ability of the microalgal consortia composed of Chlorella variabilis and Scenedesmus obliquus to treat and valorize diluted synthetic dairy wastewater under controlled laboratory conditions. The effect of time, inoculum concentration and light intensity on five responses, namely phosphate removal, ammoniacal nitrogen removal, COD removal, biomass productivity and lutein content, are studied by response surface methodology utilizing central composite design. The quadratic models are found to be suitable for phosphate removal, ammoniacal nitrogen removal, COD removal and biomass productivity. At optimized experimental conditions, the microalgal consortia exhibited phosphate removal of 70.19%, ammoniacal nitrogen removal of 86.22%, COD removal of 54.72%, biomass productivity of 29.13 mg/L/day and lutein content of 12.59 mg/g respectively. This study is of high importance as the lutein content exhibited by the microalgal consortia is higher when compared to other microalgal species and could be considered in the future as a commercial source of lutein.

Effect of pulsed ultrasound, a green food processing technique, on the secondary structure and in-vitro digestibility of almond milk protein.

In recent years, almond has been considered as one of the most common alternative plant-based protein sources due to its nutritional attributes and health benefits. However, almond protein has a lower digestibility compared with the animal protein. The objective of this study is to investigate the impact of pulsed high-intensity ultrasound on the secondary structure of the almond protein. The changes in the in-vitro protein digestibility (IVPD %) are also evaluated to investigate the relationship between the structure and digestibility of the almond protein. The secondary structures were analyzed using Fourier-transform infrared spectroscopy (FT-IR) and circular dichroism (CD) spectroscopy. FT-IR analysis showed a slight relocation in the ordered and unordered structures in the ultrasonicated almond protein compared to the control. CD spectroscopy revealed that ultrasound resulted in the restructuring of α-helices into ß-sheets. However, upon treating the almond protein for 16 min, a slight recovery in α-helices was observed. Moisture content was found to affect the secondary structure orientations of almond protein significantly. Although the IVPD% change was not statistically significant, it was found to be increasing slightly with processing duration and was dependent on protein secondary structure.