Comparison of surface roughness of additively manufactured implant-supported interim crowns fabricated with different print orientations.

PURPOSE: To assess the influence of print orientation on the surface roughness of implant-supported interim crowns manufactured by using digital light processing (DLP) 3D printing procedures. MATERIALS AND METHODS: An implant-supported maxillary right premolar full-contour crown was obtained. The interim restoration design was used to fabricate 30 specimens with 3 print orientations (0, 45, and 90 degrees) using an interim resin material (GC Temp PRINT) and a DLP printer (Asiga MAX UV) (n = 10). The specimens were manufactured, and each was cemented to an implant abutment with autopolymerizing composite resin cement (Multilink Hybrid Abutment). Surface roughness was assessed on the buccal surface of the premolar specimen by using an optical measurement system (InfiniteFocusG5 plus). The data were analyzed with a Shapiro-Wilk test, resulting in a normal distribution. One-way ANOVA and the Tukey HSD tests were selected (α = 0.05). RESULTS: Statistically significant discrepancies were found in the surface roughness mean values among the groups tested (p < 0.001). The lowest mean ± standard deviation surface roughness was found with the 90-degree group (1.2 ± 0.36 µm), followed by the 0-degree orientation (2.23 ± 0.18 µm) and the 45-degree group (3.18 ± 0.31 µm). CONCLUSIONS: Print orientation parameter significantly impacted the surface roughness of the implant-supported interim crowns manufactured by using the additive procedures tested.

Influence of the Use of Transepithelial Abutments vs. Titanium Base Abutments on Microgap Formation at the Dental Implant-Abutment Interface: An In Vitro Study.

This in vitro study aimed to assess the presence of microgaps at the implant-abutment interface in monolithic zirconia partial implant-supported fixed prostheses on transepithelial abutments versus Ti-base abutments. METHODS: Sixty conical connection dental implants were divided into two groups (n = 30). The control group consisted of three-unit bridge monolithic zirconia connected to two implants by a transepithelial abutment. The test group consisted of monolithic zirconia three-unit restoration connected to two implants directly by a titanium base (Ti-base) abutment. The sample was subjected to thermocycling (10,000 cycles at 5 °C to 55 °C, dwelling time 50 s) and chewing simulation (300,000 cycles, under 200 N at frequencies of 2 Hz, at a 30° angle). The microgap was evaluated at six points (mesiobuccal, buccal, distobuccal, mesiolingual, lingual, and distolingual) of each implant-abutment interface by using a scanning electron microscope (SEM). The data were analyzed using the Mann-Whitney U tests (p > 0.05). RESULTS: The SEM analysis showed a smaller microgap at the implant-abutment interface in the control group (0.270 µm) than in the test group (3.902 µm). Statistically significant differences were observed between both groups (p < 0.05). CONCLUSIONS: The use or not of transepithelial abutments affects the microgap size. The transepithelial abutments group presented lower microgap values at the interface with the implant than the Ti-base group in monolithic zirconia partial implant-supported fixed prostheses. However, both groups had microgap values within the clinically acceptable range.

Accuracy, scanning time, and patient satisfaction of stereophotogrammetry systems for acquiring 3D dental implant positions: A systematic review.

PURPOSE: To evaluate accuracy, scanning time, and patient satisfaction of photogrammetry (PG) systems for recording the 3D position of dental implants. MATERIAL AND METHODS: A literature search was completed in five databases: PubMed/Medline, Scopus, Embase, World of Science, and Cochrane. A manual search was also conducted. Studies reporting the use of commercially available PG systems were included. Two investigators evaluated the studies independently by applying the Joanna Briggs Institute critical appraisal. A third examiner was consulted to resolve any lack of consensus. RESULTS: A total of 14 articles were included: 3 in vivo, 6 in vitro, and 6 case report manuscripts. One clinical study evaluated trueness, another one tested precision, and the third one assessed impression time and patient and operator satisfaction. All the in vitro studies evaluated the trueness and precision of a PG system. Additionally, all the reviewed studies investigated completely edentulous conditions with multiple implants. The number of placed implants per arch among the reviewed clinical studies varied from 4 to 8 implants, while the number of implants placed on the reference casts included 4, 5, 6, or 8 implants. Not all the studies compared the accuracy of PG systems with conventional impression methods, using intraoral scanners as additional experimental groups. For the PIC system, trueness ranged from 10 to 49 µm and precision ranged from 5 to 65 µm. For the iCam4D system, trueness ranged from 24 to 77 µm and the precision value ranged from 2 to 203 µm. CONCLUSIONS: PG systems may provide a reliable alternative for acquiring the 3D position of dental implants. However, this conclusion should be interpreted carefully, as one study reported a mean precision value of one PG system higher than the clinically acceptable discrepancy. Lower scanning time and higher patient and operator satisfaction have been reported when compared with conventional techniques. Further studies are needed to increase the evidence regarding the accuracy, scanning time, and patient and operator satisfaction of the commercially available PG systems.

Influence of the dental arch and number of cutting-off and rescanning mesh holes on the accuracy of implant scans in partially edentulous situations.

OBJECTIVES: To evaluate the influence of the dental arch and cutting-off and rescanning procedures on the accuracy of complete-arch implant scans in partially edentulous arches. MATERIAL AND METHODS: A maxillary and a mandibular partially edentulous typodont with implant abutment analogs placed in the right and left first molar and right central incisor sites were digitized to create reference models by using an industrial optical scanner (7 Series Desktop Scanner; Dentalwings). Two experimental groups were scanned using an intraoral scanner (IOS) (TRIOS 4; 3Shape A/S): the Maxillary group (Mx) and the Mandibular group (Mb). Four subgroups were generated depending on the number of rescanned mesh holes: No holes (Mx-G0, Mb-G0), 1 hole (Mx-G1, Mb-G1), 2 holes (Mx-G2, Mb-G2) and 3 holes (Mx-G3, Mb-G3). A 3-dimensional metrology software (Geomagic Control X; 3D Systems) was used to measure the difference between the reference and the experimental scans computing the root mean square (RMS) error calculation. Two-way ANOVA and a post-hoc Tukey test were used to analyze the trueness data (α=0.05). Levene test was used to evaluate the prevision (α=0.05). RESULTS: The Mx group obtained a trueness mean value of 54 ± 17 µm and a mean precision value of 54 ± 17 µm, while the Mb group presented a trueness mean value of 67 ± 23 µm and a mean precision value of 66 ± 22 µm. The Mx group demonstrated significantly better trueness than the Mb group (P<.001). The G0 and G1 subgroups had the highest trueness values among the subgroups tested. No significant difference was observed between G0 and G1, G1 and G2, and G2 and G3 subgroups in trueness and precision. However, the G0 had significantly better trueness and precision values compared to G2 and G3 subgroups. In addition, the G1 had significantly better trueness values than the G3 subgroup. However, the Levene test revealed no difference in the precision mean values among the subgroups tested. CONCLUSIONS: Implant scanning trueness was affected by the dental arch and the number of rescanned mesh holes using the IOS tested. A higher number of rescanned mesh holes decreased the scanning trueness. The stitching algorithm of the IOS software tested after the mesh hole scan demonstrated a significant error, especially when multiples mesh holes are involved in the same arch. CLINICAL SIGNIFICANCE: Given that cutting-off and rescanning techniques can reduce trueness, clinicians should consider whether these techniques are necessary in complete digital workflows. This is particularly important when fabricating multiple single implant-supported restorations in the same arch.

Root microbiome diversity and structure of the Sonoran desert buffelgrass (Pennisetum ciliare L.).

Buffelgrass (Pennisetum ciliare) is an invasive plant introduced into Mexico's Sonoran desert for cattle grazing and has converted large areas of native thorn scrub. One of the invasion mechanisms buffelgrass uses to invade is allelopathy, which consists of the production and secretion of allelochemicals that exert adverse effects on other plants' growth. The plant microbiome also plays a vital role in establishing invasive plants and host growth and development. However, little is known about the buffelgrass root-associated bacteria and the effects of allelochemicals on the microbiome. We used 16S rRNA gene amplicon sequencing to obtain the microbiome of buffelgrass and compare it between samples treated with root exacknudates and aqueous leachates as allelochemical exposure and samples without allelopathic exposure in two different periods. The Shannon diversity values were between H' = 5.1811-5.5709, with 2,164 reported bacterial Amplicon Sequence Variants (ASVs). A total of 24 phyla were found in the buffelgrass microbiome, predominantly Actinobacteria, Proteobacteria, and Acidobacteria. At the genus level, 30 different genera comprised the buffelgrass core microbiome. Our results show that buffelgrass recruits microorganisms capable of thriving under allelochemical conditions and may be able to metabolize them (e.g., Planctomicrobium, Aurantimonas, and Tellurimicrobium). We also found that the community composition of the microbiome changes depending on the developmental state of buffelgrass (p = 0.0366; ANOSIM). These findings provide new insights into the role of the microbiome in the establishment of invasive plant species and offer potential targets for developing strategies to control buffelgrass invasion.

Influence of arch location and scanning pattern on the scanning accuracy, scanning time, and number of photograms of complete-arch intraoral digital implant scans.

OBJECTIVES: To measure the influence of arch location and scanning pattern on the accuracy, scanning time, and number of photograms of complete-arch implant scans acquired using an intraoral scanner (IOS). MATERIALS AND METHODS: A maxillary (maxillary group) and mandibular (mandibular group) model with 6 implant abutments on each cast was digitized using a desktop scanner (control scans). Six subgroups were created based on the scanning pattern used to acquire the scans using an IOS (Trios 4): occluso-buccal-lingual (OBL subgroup), occluso-linguo-buccal (OLB subgroup), bucco-linguo-occlusal (BLO subgroup), linguo-buccal-occlusal (LBO subgroup), zigzag (ZZ subgroup), and circumferential (C subgroup). The control scans were used as a reference to measure the discrepancy with the experimental scans calculating the root mean square error. Two-way ANOVA and the pairwise comparison Tukey tests were used to analyze the data (α = .05). RESULTS: Significant discrepancies in trueness (p < .001), precision (p < .001), scanning time (p < .001), and number of photograms (p < .001) were found. The maxillary group obtained poorer trueness and precision values, higher scanning times, and a larger number of photograms than the mandibular group. The C subgroup obtained the best trueness and precision values, but was not significantly different from the OLB, BLO, and LBO subgroups. The ZZ subgroup obtained the worst trueness and precision values (p < .05). The C subgroup obtained the lowest scanning time and number of photograms (p < .05). CONCLUSIONS: Arch location and scanning pattern influenced scanning accuracy, scanning time, and number of photograms of complete-arch implant scans.

Non-chlorophyllous and crypto-chlorophyllous fern spores differ in their mobilisation of fatty acids during priming.

During fern spore germination, lipid hydrolysis primarily provides the energy to activate their metabolism. In this research, fatty acids (linoleic, oleic, palmitic and stearic) were quantified in the spores exposed or not to priming (hydration-dehydration treatments). Five fern species were investigated, two from xerophilous shrubland and three from a cloud forest. We hypothesised that during the priming hydration phase, the fatty acids profile would change in concentration, depending on the spore type (non-chlorophyllous and crypto-chlorophyllous). The fatty acid concentration was determined by gas chromatograph-mass spectrometer. Chlorophyll in spores was vizualised by epifluorescence microscopy and quantified by high-resolution liquid chromatography with a DAD-UV/Vis detector. Considering all five species and all the treatments, the oleic acid was the most catabolised. After priming, we identified two patterns in the fatty acid metabolism: (1) in non-chlorophyllous species, oleic, palmitic, and linoleic acids were catabolised during imbibition and (2) in crypto-chlorophyllous species, these fatty acids increased in concentration. These patterns suggest that crypto-chlorophyllous spores with homoiochlorophylly (chlorophyll retained after drying) might not require the assembly of new photosynthetic apparatus during dark imbibition. Thus, these spores might require less energy from pre-existing lipids and less fatty acids as 'building blocks' for cell membranes than non-chlorophyllous spores, which require de novo synthesis and structuring of the photosynthetic apparatus.

Techniques to improve the accuracy of complete arch implant intraoral digital scans: A systematic review.

STATEMENT OF PROBLEM: The best method of optimizing the accuracy of complete arch intraoral digital scans is still unclear. For instance, the location of the scan bodies can be significantly distorted with respect to their actual positions, which would lead to a nonpassive fit of the definitive prosthesis. PURPOSE: The purpose of this systematic review was to analyze available techniques for improving the accuracy of digital scans in implant-supported complete arch fixed prostheses. MATERIAL AND METHODS: Three databases (Medline, Embase, and Google Scholar) were searched, and the results obtained were supplemented by a hand search. Specific descriptors identified techniques whose objective were to increase the accuracy of digital scans in implant-supported complete arch fixed prostheses. Titles and abstracts were screened by 2 independent reviewers, and unclear results were discussed with a third independent reviewer. A qualitative analysis based on procedural parameters was used. The interexaminer agreements of both were assessed by the Cohen kappa statistic, and the Risk of Bias Tool was used to assess the risk of bias across the studies. RESULTS: A total of 17 techniques matching the inclusion criteria were evaluated. Higher accuracy but also differences regarding the need for supplementary devices, number of intraoral scans, and time consumption of clinical and software program steps were observed compared with the conventional digital scanning protocol. The use of a splinting device was common to most of the studies. The outcome variables for the evaluation of the effectiveness of these protocols were heterogeneous. CONCLUSIONS: The use of additional techniques during intraoral scanning can improve accuracy in implant-supported complete arch fixed prostheses. However, higher complexity for those procedures should be expected.

Influence of the surface humidity, implant angulation, and interimplant distance on the accuracy and scanning time of complete-arch implant scans.

OBJECTIVES: To assess the influence of implant angulation, humidity, and interimplant distance on the accuracy and scanning time of complete-arch implant scans. METHODS: A definitive cast with 4 parallel implant abutment analogs (P group), and another cast with 4 angulated (up to 30 degrees) implant abutment analogs (NP group) were digitized by using a scanner (7Series) (reference scans). Two subgroups were created: dry (D subgroup) and wet (W subgroup). For the D subgroup, the casts were digitized without altering the surface humidity by using an intraoral scanner (IOS) (TRIOS 3). For the W subgroup, the cast surface was humidified with artificial saliva and digitized by using the same IOS. The interimplant distance discrepancies were assessed by computing linear and angular measurements. Trueness data was analyzed using 3-way ANOVA followed by the pairwise comparison Tukey tests. The Bartlett test, followed by the pairwise comparison tests, was used to assess the precision (α=.05). RESULTS: Regarding the trueness, implant angulation (P<.001) and inter-implant distance measurement (P<.001) influenced the linear discrepancies. Implant angulation (P=.002), humidity conditions (P<.001), and inter-implant distance (P=.001) influenced the angular discrepancies. Regarding the precision, significant differences in the variance of linear and angular measurements and inter-implant distances were found. Humidity conditions (P<.001) influenced the scanning time. CONCLUSIONS: Implant angulation, humidity, and interimplant distance influenced the accuracy and scanning time of complete-arch implant scans. Parallel implants resulted in higher trueness and precision values. Dry conditions resulted in slightly higher scanning trueness and precision and shorter scanning time. CLINICAL SIGNIFICANCE: Drying the surface being scanned increases intraoral scanning accuracy and decreases intraoral scanning time.

Accuracy, scanning time, and number of photograms of various scanning patterns for the extraoral digitalization of complete dentures by using an intraoral scanner.

STATEMENT OF PROBLEM: The use of intraoral scanners (IOSs) has been described as a method of digitizing complete dentures (CDs) extraorally; however, accuracy, scanning time, and number of photograms remain unclear. PURPOSE: The purpose of this in vitro study was to assess the accuracy, scanning time, and number of photograms of different scanning patterns for digitizing CDs extraorally with an IOS. MATERIAL AND METHODS: The virtual design of a maxillary and mandibular CD was used to manufacture milled CDs. The maxillary (Mx group) and mandibular (Mb group) CDs were digitized extraorally by using an IOS (Medit i500). Three subgroups were created depending on the scanning pattern: using the protocol of the IOS selected (Medit Link) (ML subgroup), using the technique recommended by the implant manufacturer (DIOnavi) (DIO subgroup), and using a custom method (C subgroup). The design of the CDs (reference files) was used to compare the discrepancy between each virtual design and the corresponding scans by using the root mean square (RMS) error. The scanning time and number of photograms were recorded. Two-way ANOVA and Tukey tests were used to analyze the trueness data. The Bartlett test was used to analyze the precision values (α=.05). RESULTS: The group (P<.001) was a significant predictor of trueness. The Mx group (RMS mean of 0.452 mm) obtained higher trueness than the Mb group (RMS mean of 0.536 mm). The Mx-C and Mb-DIO subgroups obtained the highest trueness, and the Mx-ML and Mb-ML subgroups showed the lowest trueness. Significant differences were found in precision among groups (P<.01) and across subgroups (P<.01). The Mb group obtained the highest precision mean (0.586 mm) and was significantly different from the Mx group that obtained the lowest precision mean (0.611 mm). The C subgroup obtained the highest precision mean (0.339 mm) and was significantly different from the DIO subgroup that obtained the lowest precision mean (0.425 mm). The group (P<.01) and scanning pattern (P<.01) were significant predictors of the scanning time and number of photograms obtained. The Mb group showed lower scanning time and number of photograms than the Mx group. CONCLUSIONS: The scanning patterns tested significantly influenced the trueness and precision values, scanning time, and number of photograms of the extraoral digitalization of maxillary and mandibular CDs by using the IOS tested.

Fosfomycin and Its Derivatives: New Scale Inhibitors for Oilfield Applications.

Aminomethylenephosphonate-based scale inhibitors (SIs) have been widely studied and recognized for several decades to mitigate various oilfield scales. However, most of these compounds afforded several drawbacks, such as poor biodegradability and intolerance with the production system. As environmental regulations become more rigid, new production chemicals must adhere to certain criteria to qualify for use in the oil and gas industry, particularly in areas with strict regulations, such as the Norwegian Sea. The low toxicity of fosfomycin encouraged us to test fosfomycin and related molecules as new aminomethylene-free phosphonate SIs for calcite and gypsum scales. The tested chemicals are fosfomycin disodium salt (SI-1), fosfomycin trometamol (SI-2), and hydrolysis of fosfomycin called 1,2-dihydroxypropyl phosphonic acid (SI-3). The inhibition efficiency of all these chemicals was evaluated against calcite and gypsum scales compared to commercial oilfield scale inhibitor hydroxyphosphonoacetic acid (HPAA) according to the NACE Standard TM0374-2007. In addition, the calcite scale inhibition efficiency of all aminomethylene-free phosphonate SIs (SI-1 to SI-3 and HPAA) was investigated based on the Heidrun oilfield, Norway. Moreover, we have reported the calcium compatibility of these chemicals at various concentrations of SIs and calcium ions at 80 °C over 24 h. All new aminomethylene-free phosphonate SIs showed good gypsum and calcite inhibition performance. It was also found that all tested chemicals derived from fosfomycin demonstrated excellent compatibility with calcium ions of up to 1000 ppm throughout the 24 h experiment period compared to HPAA.

Influence of the implant scan body bevel location, implant angulation and position on intraoral scanning accuracy: An in vitro study.

PURPOSE: To assess the influence of the scan body geometry bevel location and implant angulation and position of complete-arch implant digital scans. MATERIAL AND METHODS: Two definitive casts with 4 implant analogs placed parallel (P group) or angulated up to 30° (NP group) were fabricated. Five subgroups were created based on the scan body geometry bevel position: facial, mesial, distal, lingual, or random (F, M, D, L, and R subgroup). Casts were digitized using a laboratory scanner (reference) (7Series Desktop Scanner) and an intraoral scanner (TRIOS 3). The implant position discrepancies between the reference and experimental scans were calculated. Data was analyzed using 3-way ANOVA and Tukey tests (α = .05). RESULTS: The scan body geometry bevel position (P < .001) and the inter-implant distance (P < .001) were shown as significant predictors of the linear discrepancies obtained. The L subgroup had a significantly lower discrepancy compared with the other subgroups. Implant angulation (P < .001), the scan body geometry bevel position (P < .001), and the inter-implant distance (P < .001) were all significant predictors on the angular discrepancies obtained. CONCLUSIONS: The scan body geometry bevel location and implant angulation and position influenced the accuracy of the IOS tested. The lingual orientation obtained significantly better accuracy values compared with the other positions. The parallel implant analog position obtained better accuracy than the angulated positions. Lastly, the implant positioned in the dental arch where the intraoral digital scan was finished obtained significantly higher distortion than the contralateral implant.

Antihyperglycemic Effects of Salvia polystachya Cav. and Its Terpenoids: α-Glucosidase and SGLT1 Inhibitors.

The antihyperglycemic activity of ethanolic extract from Salvia polystachya (EESpS) and its products was evaluated using in vivo, ex vivo and in silico assays; additionally, an acute toxicity assay was evaluated. EESpS was classified as a nontoxic class 5 drug. EESpS, ethyl acetate fraction (EtOAcFr), secondary-6-fraction (SeFr6), ursolic acid (UA), and oleanolic acid (OA) reduced the hyperglycemia in DM2 mice. α-glucosidase inhibition was evaluated with oral sucrose and starch tolerance tests (OSuTT and OStTT), an intestinal sucrose hydrolysis (ISH) assay and molecular docking studies using acarbose as control. SGLT1 inhibition was evaluated with oral glucose and galactose tolerance tests (OGTT and OGaTT), an intestinal glucose absorption (IGA) assay and molecular docking studies using canagliflozin as the control. During the carbohydrate tolerance tests, all the treatments reduced the postprandial peak, similar to the control drugs. During the ISH, IC50 values of 739.9 and 726.3 µM for UA and OA, respectively, were calculated. During the IGA, IC50 values of 966.6 and 849.3 for UA, OA respectively, were calculated. Finally, during the molecular docking studies, UA and OA showed ∆G values of -6.41 and -5.48 kcal/mol-1, respectively, on α-glucosidase enzymes. During SGLT1, UA and OA showed ∆G values of -10.55 and -9.65, respectively.

Influence of implant angulation and clinical implant scan body height on the accuracy of complete arch intraoral digital scans.

STATEMENT OF PROBLEM: The accuracy of digital implant scans can be affected by the implant angulation, implant depth, or interimplant distance. However, studies analyzing intraoral scanning accuracy with different implant angulations and different scan body heights are scarce. PURPOSE: The purpose of this in vitro study was to determine the influence of the implant angulation and clinical implant scan body height on the accuracy of complete arch scans. MATERIAL AND METHODS: Two definitive implant casts with 6 implant analogs (Zimmer Biomet) were obtained: 1 cast had all the implant analogs parallel (GP group), and 1 cast had the implant analogs with divergence of up to 30 degrees (GD group). A coordinate measurement machine (Global Evo 09.15.08) was used to measure the positions of the implant analogs. Each group was divided into 3 subgroups depending on the clinical implant scan body height: 10, 6, and 3 mm. An implant scan body (Elos Accurate Scan Body Brånemark system) was positioned on each implant analog. A total of 10 scans of each subgroup were recorded by using an intraoral scanner (TRIOS 3). Each STL file obtained was imported into a reverse engineering software program (Geomagic), and linear and angular Euclidean measurements were obtained. The Euclidean calculations between the implant analog positions of the definitive implant casts were used as a reference to calculate the discrepancies among the corresponding subgroups. The Kolmogorov-Smirnov test revealed that the lineal measurements were not normally distributed, so the Kruskal-Wallis and pairwise comparison Dunn tests were used (α=.05). The Kolmogorov-Smirnov test revealed that the angular measurements were normally distributed. Therefore, the 2-way ANOVA and pairwise comparison Tukey tests were used (α=.05). RESULTS: The Kruskal-Wallis test revealed significant differences in the linear Euclidean medians between the GP and GD groups with different clinical implant scan body heights (H(5)=23.18, P<.001). Significant differences in the linear Euclidean medians were computed between the GP-6 and GD-10 subgroups (P=.009), GD-3 and GD-6 subgroups (P=.029), and GD-3 and GD-10 subgroups (P=.001). Two-way ANOVA revealed that the implant angulation (F(1, 3.3437)=28.93, P<.001) and clinical implant scan body height (F(2, 0.4358)=3.77, P=.029) were significant predictors of discrepancies in the angular measurement. CONCLUSIONS: Implant angulation and clinical scan body height influenced scanning accuracy. The lowest clinical implant scan body height tested had the lowest accuracy in both parallel and angulated implants, but statistically significant differences were found only in the angulated group.

Squash root microbiome transplants and metagenomic inspection for in situ arid adaptations.

Arid zones contain a diverse set of microbes capable of survival under dry conditions, some of which can form relationships with plants under drought stress conditions to improve plant health. We studied squash (Cucurbita pepo L.) root microbiome under historically arid and humid sites, both in situ and performing a common garden experiment. Plants were grown in soils from sites with different drought levels, using in situ collected soils as the microbial source. We described and analyzed bacterial diversity by 16S rRNA gene sequencing (N = 48) from the soil, rhizosphere, and endosphere. Proteobacteria were the most abundant phylum present in humid and arid samples, while Actinobacteriota abundance was higher in arid ones. The ß-diversity analyses showed split microbiomes between arid and humid microbiomes, and aridity and soil pH levels could explain it. These differences between humid and arid microbiomes were maintained in the common garden experiment, showing that it is possible to transplant in situ diversity to the greenhouse. We detected a total of 1009 bacterial genera; 199 exclusively associated with roots under arid conditions. By 16S and shotgun metagenomics, we identified dry-associated taxa such as Cellvibrio, Ensifer adhaerens, and Streptomyces flavovariabilis. With shotgun metagenomic sequencing of rhizospheres (N = 6), we identified 2969 protein families in the squash core metagenome and found an increased number of exclusively protein families from arid (924) than humid samples (158). We found arid conditions enriched genes involved in protein degradation and folding, oxidative stress, compatible solute synthesis, and ion pumps associated with osmotic regulation. Plant phenotyping allowed us to correlate bacterial communities with plant growth. Our study revealed that it is possible to evaluate microbiome diversity ex-situ and identify critical species and genes involved in plant-microbe interactions in historically arid locations.

Intraoral Digital Scans for Fabricating Tooth-Supported Prostheses Using a Custom Intraoral Scan Body.

This article describes a technique to assist with intraoral digital scans for fabricating tooth-supported prostheses by using a custom intraoral scan body when the extension of the scan or the clinical characteristics might compromise the reliability of the intraoral digital scan. A preliminary intraoral scan of the tooth preparations is used to design a custom intraoral scan body which is manufactured using polymethylmethacrylate and a 5-axis milling machine. A low-viscosity polyvinyl siloxane impression of the tooth preparations is obtained using the custom intraoral scan body. Subsequently, the custom intraoral scan body is digitized using an intraoral scanner. A design software program is used to align the digitized custom intraoral scan body with the preliminary intraoral scan to obtain the definitive virtual cast. This technique aims to reduce manual conventional laboratory procedures such as pouring dental impression or die trimming which might minimize inaccuracies on the virtual definitive cast.

Merging intraoral scans and CBCT: a novel technique for improving the accuracy of 3D digital models for implant-supported complete-arch fixed dental prostheses.

AIM: A technique for merging digital intraoral and CBCT scans for implant-supported complete-arch fixed dental prostheses (FDPs) is described. The aim is to improve the dimensional accuracy of intraoral scans in edentulous arches. MATERIALS AND METHOD: Two files are recorded: an intraoral scan and a CBCT scan, both obtained with scan bodies connected to the implants in the same position. The intraoral scan is then divided into several fragments and realigned, taking as reference the position of the implants recorded in the CBCT file. RESULTS: An improved intraoral digital model with corrected implant positions appropriate for complete-arch implant FDPs is generated. CONCLUSION: The methodology proposed can minimize possible intraoral scanning error and deliver more reliable digital impressions for implant-supported complete-arch FDPs.

Metal and Metalloid Toxicity in Plants: An Overview on Molecular Aspects.

Worldwide, the effects of metal and metalloid toxicity are increasing, mainly due to anthropogenic causes. Soil contamination ranks among the most important factors, since it affects crop yield, and the metals/metalloids can enter the food chain and undergo biomagnification, having concomitant effects on human health and alterations to the environment. Plants have developed complex mechanisms to overcome these biotic and abiotic stresses during evolution. Metals and metalloids exert several effects on plants generated by elements such as Zn, Cu, Al, Pb, Cd, and As, among others. The main strategies involve hyperaccumulation, tolerance, exclusion, and chelation with organic molecules. Recent studies in the omics era have increased knowledge on the plant genome and transcriptome plasticity to defend against these stimuli. The aim of the present review is to summarize relevant findings on the mechanisms by which plants take up, accumulate, transport, tolerate, and respond to this metal/metalloid stress. We also address some of the potential applications of biotechnology to improve plant tolerance or increase accumulation.

Metagenomics of mine tailing rhizospheric communities and its selection for plant establishment towards bioremediation.

Mining operations often generate tailing dams that contain toxic residues and are a source of contamination when left unconfined. The establishment of a plant community over the tailings has been proposed as a containment strategy known as phytostabilization. Previously, we described naturally occurring mine tailing colonizing plants such as Acacia farnesiana, Brickellia coulteri, Baccharis sarothroides, and Gnaphalium leucocephalum without finding local adaptation. We explored the rhizosphere microbes as contributors in plant establishment and described both the culturable and in situ diversity of rhizospheric bacteria using the 16S rRNA gene and metagenomic shotgun sequencing. We built a synthetic community (SC) of culturable rhizosphere bacteria from the mine tailings. The SC was then the foundation for a serial passes experiment grown in plant-derived nutrient sources, selecting for heavy metals tolerance, community cooperation, and competition. The outcome of the serial passes was named the 'final synthetic community' (FSC). Overall, diversity decreased from in situ uncultivable microbes from roots (399 bacteria genera) to the cultivated communities (291 genera), the SC (94 genera), and the lowest diversity was in the FSC (43 genera). Metagenomic diversity clustered into 94,245 protein families, where we found plant growth promotion-related genes such as the csgBAC and entCEBAH, coded in a metagenome-assembled genome named Kosakonia sp. Nacozari. Finally, we used the FSC to inoculate mine tailing colonizing plants in a greenhouse experiment. The plants with the FSC inocula observed higher relative plant growth rates in sterile substrates. The FSC presents promising features that might make it useful for phytostabilization tailored strategies.