Intraindividual Relationships Between Training Loads and Heart-Rate Variability in High-Level Female Futsal Players: A Longitudinal Study.

PURPOSE: To investigate the internal training loads of a professional Spanish female futsal team throughout 26 weeks of training including preseason and in-season weeks and verify the impact of training period and/or training load magnitudes on heart-rate variability responses. Furthermore, we aimed to assess, intraindividually, the relationship between training load and the coefficient of variation (CV) of weekly natural log of the root mean square difference of successive normal interbeat (RR) intervals (lnRMSSDCV), obtained from ∼5 measures per week, and recorded in the seated position. METHODS: A within-subject design involved 12 high-level outfield female futsal players (mean [SD] age: 23.9 [3.4] y). RESULTS: lnRMSSD was significantly lower and lnRMSSDCV was significantly higher during the preseason (weeks 1-6) compared to in-season (weeks 7-26) (P < .001). Individually, players presented moderate to large negative correlations between lnRMSSDCV and lnRMSSD during the 26 weeks of observation. Correlations ranged between rplayer4 = -.41 (95% CI, -.69 to -.02) and rplayer12 = -.55 (-.78 to -.18). Players also presented moderate to very large positive correlations between lnRMSSDCV and session rating of perceived exertion. Correlations ranged between rplayer7 = .41 (.04 to .71) and rplayer1 = .71 (.45 to .86). CONCLUSION: Professional female futsal players in this study presented increased lnRMSSD and reduced lnRMSSDCV during 20 weeks into the competitive season compared to 6 weeks of preseason. Furthermore, lnRMSSDCV was negatively associated with lnRMSSD on an intraindividual basis. Finally, higher internal training loads were positively correlated with lnRMSSDCV, indicating that heart-rate variability is responsive to weekly training loads.

Analysis of key external and internal load variables in professional female futsal players: a longitudinal study.

The aims of this study were to identify the key external and internal load variables in professional futsal through principal components analysis (PCA), and analyse the physical performance required by the players in official matches. Data were collected from 14 female players during 10 matches using WIMU PROTM. The PCA selected a total of 22 variables as key indicators of players' load. Specifically, these variables were represented by five principal components. However, a novel finding was that different components were extracted when the analysis was carried out by full match (68.83% of total variance), first half (69.81% of total variance), or second half (65.96% of total variance). Also, this study found that the players decreased their physical performance during the second half. Based on these results, this study may help optimize performance and reduce the injury risk. Performance should not be only analysed considering the full match external/internal load but also specifying by match halves. This is explained by the fact that there were variables that made up the principal components in the first half, but not in the second half or full match. Finally, coaches should adopt training strategies which deal with the decrease in physical performance during the second half.

The Most Demanding Exercise in Different Training Tasks in Professional Female Futsal: A Mid-Season Study through Principal Component Analysis.

The contextual factors related to training tasks can play an important role in how a player performs and, subsequently, in how a player trains to face a competition. To date, there has been no study that has investigated the most demanding exercise in different training tasks in female futsal. Therefore, this study aimed to determine the most demanding efforts during different training tasks in a cohort study conducted in professional biological women futsal players using principal component analysis (PCA). A total of 14 elite women futsal players (age = 24.34 ± 4.51 years; height = 1.65 ± 0.60 m; body mass = 63.20 ± 5.65 kg) participated in this study. Seventy training sessions of an elite professional women's team were registered over five months (pre-season and in-season). Different types of exercises were grouped into six clusters: preventive exercises; analytical situations; exercises in midcourt; exercises in ¾ of the court; exercises in full court; superiorities/inferiorities. Each exercise cluster was composed of 5-7 principal components (PCs), considering from 1 to 5 main variables forming each, explaining from 65 to 75% of the physical total variance. A total of 13-19 sub-variables explained the players' efforts in each training task group. The first PCs to explain the total variance of training load were as follows: preventive exercises (accelerations; ~31%); analytical situations (impacts; ~23%); exercises in midcourt (high-intensity efforts; ~28%); exercises in ¾ of the court (~27%) and superiorities/inferiorities (~26%) (aerobic/anaerobic components); exercises in full court (anaerobic efforts; ~24%). The PCs extracted from each exercise cluster provide evidence that may assist researchers and coaches during training load monitoring. The descriptive values of the training load support a scientific base to assist coaches in the planning of training schedules.

Application of a Parallel Synthetic Strategy in the Discovery of Biaryl Acyl Sulfonamides as Efficient and Selective NaV1.7 Inhibitors.

The majority of potent and selective hNaV1.7 inhibitors possess common pharmacophoric features that include a heteroaryl sulfonamide headgroup and a lipophilic aromatic tail group. Recently, reports of similar aromatic tail groups in combination with an acyl sulfonamide headgroup have emerged, with the acyl sulfonamide bestowing levels of selectivity over hNaV1.5 comparable to the heteroaryl sulfonamide. Beginning with commercially available carboxylic acids that met selected pharmacophoric requirements in the lipophilic tail, a parallel synthetic approach was applied to rapidly generate the derived acyl sulfonamides. A biaryl acyl sulfonamide hit from this library was elaborated, optimizing for potency and selectivity with attention to physicochemical properties. The resulting novel leads are potent, ligand and lipophilic efficient, and selective over hNaV1.5. Representative lead 36 demonstrates selectivity over other human NaV isoforms and good pharmacokinetics in rodents. The biaryl acyl sulfonamides reported herein may also offer ADME advantages over known heteroaryl sulfonamide inhibitors.

Does inactivation of USP14 enhance degradation of proteasomal substrates that are associated with neurodegenerative diseases?

A common pathological hallmark of age-related neurodegenerative diseases is the intracellular accumulation of protein aggregates such as α-synuclein in Parkinson's disease, TDP-43 in ALS, and tau in Alzheimer's disease. Enhancing intracellular clearance of aggregation-prone proteins is a plausible strategy for slowing progression of neurodegenerative diseases and there is great interest in identifying molecular targets that control protein turnover. One of the main routes for protein degradation is through the proteasome, a multisubunit protease that degrades proteins that have been tagged with a polyubiquitin chain by ubiquitin activating and conjugating enzymes. Published data from cellular models indicate that Ubiquitin-specific protease 14 (USP14), a deubiquitinating enzyme (DUB), slows the degradation of tau and TDP-43 by the proteasome and that an inhibitor of USP14 increases the degradation of these substrates. We conducted similar experiments designed to evaluate tau, TDP-43, or α-synuclein levels in cells after overexpressing USP14 or knocking down endogenous expression by siRNA.