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In the last decade, the highest levels of greenhouse gases (GHG) in the atmosphere have been recorded, with carbon dioxide (CO2) being one of the GHGs that most concerns mankind due to the rate at which it is generated on the planet. Given its long time of permanence in the atmosphere (between 100 to 150 years); this has deployed research in the scientific field focused on the absorption and desorption of CO2 in the atmosphere. This work presents the study of CO2 adsorption employing materials based on graphene oxide (GO), such as GO foams with different oxidation percentages (3.00%, 5.25%, and 9.00%) in their structure, obtained via an environmentally friendly method. The characterization of CO2 adsorption was carried out in a closed system, within which were placed the GO foams and other CO2 adsorbent materials (zeolite and silica gel). Through a controlled chemical reaction, production of CO2 was conducted to obtain CO2 concentration curves inside the system and calculate from these the efficiency, obtained between 86.28 and 92.20%, yield between 60.10 and 99.50%, and effectiveness of CO2 adsorption of the materials under study. The results obtained suggest that GO foams are a promising material for carbon capture and the future development of a new clean technology, given their highest CO2 adsorption efficiency and yield.
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Objetivo: identificar el impacto de la medida de confinamiento social, decretada por el gobierno colombiano ante el covid-19, sobre los niveles de ansiedad y los comportamientos alimentarios de los deportistas adscritos a selecciones Antioquia. Metodología: estudio observacional descriptivo de corte transversal en deportistas de altos logros deportivos de Indeportes Antioquia que fueron atendidos en el área de medicina, entre enero y agosto 2020. Se indagó sobre aspectos de ansiedad y comportamientos alimentarios, ocasionados durante la medida de aislamiento social derivada de la pandemia por covid-19. Resultados: la encuesta fue respondida por 150 deportistas. En hombres, la ansiedad aumenta a medida que se incrementa el grupo de edad. En las mujeres, el fenómeno es opuesto. Para la misma calificación de ansiedad alta, el porcentaje va disminuyendo con la edad. Con referencia a los comportamientos relacionados con la alimentación, analizados bajo una circunstancia tan compleja, como es la pandemia por covid-19, permitió a los profesionales del deporte y la salud direccionar recomendaciones efectivas para los deportistas que asesoran, frente al manejo de la ansiedad y el control de ciertos comportamientos negativos que pueden afectar la salud y el rendimiento deportivo. Conclusiones: los resultados con respecto a la ansiedad coinciden con estudios actuales, en los que, en términos generales, las poblaciones evaluadas muestran niveles de ansiedad baja y esto se relaciona con una escasa tendencia a alteraciones en la conducta alimentaria en la población estudiada.
Objective: to identify the impact of the social confinement measure decreed by the Colombian government in the face of Covid-19 on anxiety levels and eating behaviors in athletes attached to different teams in the department of Antioquia. Methodology: cross-sectional descriptive observational study in athletes with high sports achievements from Indeportes Antioquia, who were treated in the medical area between January and August 2020. Aspects of anxiety and eating behaviors, caused during the social isolation measure derived of the Covid-19 pandemic were studied. Results: the survey was answered by 150 athletes. In men, anxiety increases as the age group increases. In women, the phenomenon is opposite. For the same high anxiety rating, the percentage decreases with age. With reference to behaviors related to eating, analyzed under such a complex circumstance, such as the Covid-19 pandemic, allowed sports and health professionals to direct effective recommendations for the athletes they advise, regarding anxiety management and the control of certain negative behaviors that can affect their health and their performance. Conclusions: the results regarding anxiety coincide with current studies, in which, in general terms, the assessed populations show low levels of anxiety; this is related to a low tendency to alterations in eating behavior in the studied population.
Objetivo: identificar o impacto da medida de confinamento social, decretada pelo governo colombiano diante da covid-19, nos níveis de ansiedade e comportamentos alimentares de atletas lotados nas equipes de Antioquia. Metodologia:estudo observacional descritivo transversal em atletas com altas conquistas esportivas do Indeportes Antioquia que foram atendidos na área médica, entre janeiro e agosto de 2020. Aspectos de ansiedade e comportamentos alimentares, causados durante a medida de isolamento social derivada do covid-19 pandemia. Resultados: a pesquisa foi respondida por 150 atletas. Nos homens, a ansiedade aumenta à medida que a faixa etária aumenta. Nas mulheres, o fenômeno é oposto. Para a mesma alta taxa de ansiedade, a porcentagem diminui com a idade. No que se refere aos comportamentos relacionados com a alimentação, analisados numa circunstância tão complexa, como é a pandemia de covid-19, permitiu aos profissionais do desporto e da saúde direcionar recomendações eficazes aos atletas que aconselham, no que diz respeito à gestão da ansiedade e ao controlo de determinados comportamentos negativos. que podem afetar a saúde e o desempenho esportivo.Conclusões:os resultados relativos à ansiedade coincidem com os estudos atuais, nos quais, em termos gerais, as populações avaliadas apresentam baixos níveis de ansiedade e isto está relacionado com uma baixa propensão a alterações do comportamento alimentar na população estudada.
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Humanos , Masculino , Femenino , Atletas , Ansiedad , Aislamiento Social , Conducta Alimentaria , COVID-19RESUMEN
Cyanobacterial molecular biology can identify pathways that affect the adhesion and settlement of biofouling organisms and, consequently, obtain novel antifouling strategies for marine applications. Proteomic analyses can provide an essential understanding of how cyanobacteria adapt to different environmental settings. However, only a few qualitative studies have been performed in some cyanobacterial strains. Considering the limited knowledge about protein expression in cyanobacteria in different growing conditions, a quantitative proteomic analysis by LC-MS/MS of biofilm cells from a filamentous strain was performed. Biofilms were also analysed through standard methodologies for following cyanobacterial biofilm development. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s-1 and 40 s-1). Biofilm development was higher at 4 s-1 and no significant differences were found between surfaces. Proteomic analysis identified 546 proteins and 41 were differentially expressed. Differences in protein expression were more noticeable between biofilms formed on glass and perspex at 4 s-1. When comparing biofilms formed on different surfaces, results suggest that biofilm development may be related to the expression of several proteins like a beta-propeller domain-containing protein, chaperone DnaK, SLH domain-containing proteins, an OMF family outer membrane protein, and/or additional uncharacterized proteins. Regarding the hydrodynamic effect, biofilm development can be related to SOD enzyme expression, to proteins related to photosynthetic processes and to a set of uncharacterized proteins with calcium binding domains, disordered proteins, and others involved in electron transfer activity. Studies that combine distinct approaches are essential for finding new targets for antibiofilm agents. The characterisation performed in this work provides new insights into how shear rate and surface affect cyanobacterial biofilm development and how cyanobacteria adapt to these different environmental settings from a macroscopic standpoint to a proteomics context.
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Cianobacterias , Proteómica , Biopelículas , Cromatografía Liquida , Espectrometría de Masas en TándemRESUMEN
Revisando un siglo de historia, el desarrollo de las actividades anatómicas en la ciudad de Concepción, en el sur de Chile, no estuvieron ajenas a dificultades. El presente trabajo reunió los antecedentes históricos que relatan los inicios y desarrollo de la enseñanza de la anatomía en la naciente Universidad de Concepción. Se realizó una búsqueda que incluyó la revisión de libros históricos, revistas, archivos digitales y registros institucionales como actas y archivos fotográficos. La fundación de la Universidad, los inicios de las actividades anatómicas en 1919 y el aporte de sus principales pioneros, evidencian las dificultades de la enseñanza de esta disciplina en Chile. Desde una perspectiva histórica, conocer dentro de este siglo de enseñanza los recursos físicos utilizados para la disección cadavérica, el detalle de las metodologías pedagógicas utilizadas y los recursos anatómicos disponibles, resultan ser un aporte al conocimiento del desarrollo de la anatomía en Chile.
Reviewing a century of history, the development of anatomical activities in the city of Concepción, in the south of Chile, did not occur without difficulties. The present work gathered the historical background related with the beginnings and development of Anatomy teaching at the Universidad de Concepcion. Research was carried out including the review of historical books, magazines, digital archives and institutional records such as files or photographic archives. The foundation of the university, the beginnings of anatomical activities in 1919 and the contribution of its highlighted pioneers, show the difficulties of teaching this discipline in Chile. From a historical perspective, having knowledge during this century of teaching, the physical resources used in cadaveric dissection, the detail of teaching methodologies applied, and the anatomical resources available, contribute the anatomy´s development knowledge in Chile.
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Historia del Siglo XX , Educación Médica/historia , Anatomía/educación , Anatomía/historia , ChileRESUMEN
Despite the widespread use of nanoparticles (NPs), there are still major gaps of knowledge regarding the impact of nanomaterials in the environment and aquatic animals. The present work aimed to study the effects of 7 and 40â¯nm gold nanoparticles (AuNPs) - citrate and polyvinylpyrrolidone (PVP) coated - on the liver proteome of the estuarine/marine fish gilthead seabream (Sparus aurata). After 96â¯h, exposure to AuNP elicited alterations on the abundance of 26 proteins, when compared to the control group. AuNPs differentially affected several metabolic pathways in S. aurata liver cells. Among the affected proteins were those related to cytoskeleton and cell structure, gluconeogenesis, amino acids metabolism and several processes related to protein activity (protein synthesis, catabolism, folding and transport). The increased abundance of proteins associated with energy metabolism (ATP synthase subunit beta), stress response (94â¯kDa glucose-regulated protein) and cytoskeleton structure (actins and tubulins) may represent the first signs of cellular oxidative stress induced by AuNPs. Although higher gold accumulation was found in the liver of S. aurata exposed to 7â¯nm PVP-AuNPs, the 7â¯nm cAuNPs were more bioactive, inducing more effects in liver proteome. Gold accumulated more in the spleen than in the other assessed tissues of S. aurata exposed to AuNPs, highlighting its potential role on the elimination of these NPs.
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Oro/toxicidad , Hígado/efectos de los fármacos , Nanopartículas del Metal/toxicidad , Proteoma/metabolismo , Dorada/metabolismo , Contaminantes Químicos del Agua/toxicidad , Animales , Ácido Cítrico/química , Hígado/metabolismo , Redes y Vías Metabólicas/efectos de los fármacos , Oxidación-Reducción , Estrés Oxidativo/efectos de los fármacos , Povidona/química , Proteómica , Propiedades de SuperficieRESUMEN
In mainland Europe, the mosquito species Culex modestus Ficalbi (1890) is a bridge vector for West Nile virus (WNV) from its natural bird-mosquito cycle to mammals. The present study assessed the genetic diversity of Cx. modestus, as well as related Culex species, using the mitochondrial COI DNA barcoding region and compared this with the population structure across Europe. A haplotype network was mapped to determine genealogical relationships among specimens. The intraspecific genetic diversity within individual Culex species was below 2%, whereas the interspecific genetic divergence varied from 2.99% to 13.74%. In total, 76 haplotypes were identified among 198 sequences. A median-joining network determined from 198 COI sequences identified two major lineages that were separated by at least four mutation steps. A high level of intraspecific genetic diversity was not detected in Cx. modestus in samples submitted from different European populations, which indicates that morphologically identified specimens represent a single species and not a species complex. Therefore, it is deduced that different populations of Cx. modestus will show a similar potential to transmit WNV, lending support to concerns that the population present in southeast England represents a risk of transmission to humans.
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Distribución Animal , Culex/fisiología , Variación Genética , Animales , Culex/genética , Complejo IV de Transporte de Electrones/análisis , Proteínas de Insectos/análisis , Reino UnidoRESUMEN
This study compares the role of hepatic cytosolic glutathione transferases (cGST) isoforms of three different bivalve species to a Microcystis aeruginosa extract and purified MC-LR exposure (both at 150 µg MC-LR L-1) for 24 h. Characterization and alterations of the cytosolic GST activities in Mytilus galloprovincialis, Ruditapes philippinarum and Corbicula fluminea were measured using four class-specific substrates and changes in individual GST isoforms expression were achieved by a subsequent two-dimensional electrophoresis analysis. Evaluation of cGST activity basal levels using the four class-specific substrates denoted quantitative differences between the three bivalves. Purified MC-LR did not induce any significant response from bivalves. On the other hand, cell extracts caused significant alterations according to bivalves and substrates. Among the three bivalves, only R. philippinarum showed a significant induction of cGST activity using generic 1-chloro-2,4-dinitrobenzene (CDNB) substrate. However, no significant alterations were detected in these clams by cell extracts using the other specific substrates. In contrast, C. fluminea revealed significant induction of cGST activity when using 3,4-dichloronitrobenzene (DCNB) and ethacrynic acid (EA). In M. galloprovincialis, cell extracts promoted a significant decrease of cGST activity when using EA substrate. Altered protein expression was quantitatively detected upon exposure to cell extracts for one spot in R. philippinarum and another for C. fluminea, both upregulated (2.0 and 8.5-fold, respectively) and identified as a sigma1-class GST in the case of the first. The results showed that the three bivalves presented specific adaptive biotransformation responses to MCs and other cyanobacteria compounds supported by the modulation of distinct cGST classes.
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Toxinas Bacterianas/toxicidad , Bivalvos/efectos de los fármacos , Glutatión Transferasa/antagonistas & inhibidores , Hígado/enzimología , Microcistinas/toxicidad , Animales , Biotransformación , Bivalvos/enzimología , Isoenzimas/antagonistas & inhibidores , Toxinas Marinas , Microcystis , Proteómica , Extractos de Tejidos/toxicidadRESUMEN
Carbonic anhydrase plays a key role in CO2 transport, acid-base and ion regulation and metabolic processes in vertebrates. While several carbonic anhydrase isoforms have been identified in numerous vertebrate species, basal lineages such as the cyclostomes have remained largely unexamined. Here we investigate the repertoire of cytoplasmic carbonic anhydrases in the sea lamprey (Petromyzon marinus), that has a complex life history marked by a dramatic metamorphosis from a benthic filter-feeding ammocoete larvae into a parasitic juvenile which migrates from freshwater to seawater. We have identified a novel carbonic anhydrase gene (ca19) beyond the single carbonic anhydrase gene (ca18) that was known previously. Phylogenetic analysis and synteny studies suggest that both carbonic anhydrase genes form one or two independent gene lineages and are most likely duplicates retained uniquely in cyclostomes. Quantitative PCR of ca19 and ca18 and protein expression in gill across metamorphosis show that the ca19 levels are highest in ammocoetes and decrease during metamorphosis while ca18 shows the opposite pattern with the highest levels in post-metamorphic juveniles. We propose that a unique molecular switch occurs during lamprey metamorphosis resulting in distinct gill carbonic anhydrases reflecting the contrasting life modes and habitats of these life-history stages.
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Anhidrasas Carbónicas , Citoplasma , Proteínas de Peces , Regulación Enzimológica de la Expresión Génica/fisiología , Petromyzon , Animales , Dióxido de Carbono/metabolismo , Anhidrasas Carbónicas/biosíntesis , Anhidrasas Carbónicas/genética , Citoplasma/enzimología , Citoplasma/genética , Proteínas de Peces/biosíntesis , Proteínas de Peces/genética , Branquias/enzimología , Petromyzon/genética , Petromyzon/metabolismoRESUMEN
Harmful algal blooms (HABs) contaminate aquatic ecosystems and are responsible for animal poisoning worldwide. We conducted a toxicity test with the aquatic fern and the biofertilizer, Azolla filiculoides. The sporophytes were exposed to three concentrations (0.01, 0.1 and 1µgmL(-1)) of a microcystin (MC) cyanobacterial crude extract and purified MC-LR. The growth of A. filiculoides decreased only at 1µgmL(-1) crude extract concentration while with MC-LR it decreased at all the tested concentrations, indicating that the presence of other compounds in the crude extract altered toxicity and stimulated the fern growth at lower concentrations (0.01 and 0.1µgmL(-1)). Both phycoerythrocyanin and allophycocyanin levels decreased in all the concentrations of crude extract and MC-LR. The phycocyanin had a marked increase at 0.1µgmL(-1) crude extract concentration and a marked decrease at 1µgmL(-1) MC-LR concentration. These changes in the phycobiliprotein content indicate a shift in the antenna pigments of the cyanobionts of A. filiculoides. The changes in two oxidative stress enzymes, glutathione reductase for the crude extract assay and glutathione peroxidase for MC-LR assay, points towards the induction of stress defense responses. The low bioconcentration factor in both crude extract and MC-LR treatments can suggest the low uptake of microcystins, and indicates that the aquatic fern can be used as a biofertilizer and as animal feed but is not suitable for MC phytoremediation.
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Helechos/fisiología , Microcistinas/toxicidad , Simbiosis , Contaminantes Químicos del Agua/toxicidad , Anabaena/química , Alimentación Animal , Antioxidantes/metabolismo , Biodegradación Ambiental , Relación Dosis-Respuesta a Droga , Helechos/efectos de los fármacos , Helechos/crecimiento & desarrollo , Fertilizantes , Proteínas de Plantas/metabolismo , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/fisiologíaRESUMEN
Mutations of the PARK2 and PINK1 genes, encoding the cytosolic E3 ubiquitin-protein ligase Parkin and the mitochondrial serine/threonine kinase PINK1, respectively, cause autosomal recessive early-onset Parkinson's disease (PD). Parkin and PINK1 cooperate in a biochemical mitochondrial quality control pathway regulating mitochondrial morphology, dynamics and clearance. This study identifies the multifunctional PD-related mitochondrial matrix enzyme 17-ß hydroxysteroid dehydrogenase type 10 (HSD17B10) as a new Parkin substrate. Parkin overproduction in cells increased mitochondrial HSD17B10 abundance by a mechanism involving ubiquitin chain extension, whereas PARK2 downregulation or deficiency caused mitochondrial HSD17B10 depletion in cells and mice. HSD17B10 levels were also found to be low in the brains of PD patients with PARK2 mutations. Confocal and Förster resonance energy transfer (FRET) microscopy revealed that HSD17B10 recruited Parkin to the translocase of the outer membrane (TOM), close to PINK1, both in functional mitochondria and after the collapse of mitochondrial membrane potential (ΔΨm). PD-causing PARK2 mutations impaired interaction with HSD17B10 and the HSD17B10-dependent mitochondrial translocation of Parkin. HSD17B10 overproduction promoted mitochondrial elongation and mitigated CCCP-induced mitochondrial degradation independently of enzymatic activity. These effects were abolished by overproduction of the fission-promiting dynamin-related protein 1 (Drp1). By contrast, siRNA-mediated HSD17B10 silencing enhanced mitochondrial fission and mitophagy. These findings suggest that the maintenance of appropriate mitochondrial HSD17B10 levels is one of the mechanisms by which Parkin preserves mitochondrial quality. The loss of this protective mechanism may contribute to mitochondrial dysfunction and neuronal degeneration in autosomal recessive PD.
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3-Hidroxiacil-CoA Deshidrogenasas/metabolismo , Encéfalo/metabolismo , Mitocondrias/fisiología , Enfermedad de Parkinson/metabolismo , Ubiquitina-Proteína Ligasas/metabolismo , 3-Hidroxiacil-CoA Deshidrogenasas/genética , Animales , Encéfalo/fisiopatología , Regulación de la Expresión Génica , Humanos , Ratones , Mitocondrias/metabolismo , Proteínas de Transporte de Membrana Mitocondrial/metabolismo , Proteínas Mitocondriales/metabolismo , Recambio Mitocondrial , Mutación , Enfermedad de Parkinson/fisiopatología , Ratas , Ubiquitina-Proteína Ligasas/genética , UbiquitinaciónRESUMEN
The potential protective effect of the dietary antioxidant curcumin (120 mg/Kg/day for 6 days) against the renal injury induced by maleate was evaluated. Tubular proteinuria and oxidative stress were induced by a single injection of maleate (400 mg/kg) in rats. Maleate-induced renal injury included increase in renal vascular resistance and in the urinary excretion of total protein, glucose, sodium, neutrophil gelatinase-associated lipocalin (NGAL) and N-acetyl ß-D-glucosaminidase (NAG), upregulation of kidney injury molecule (KIM)-1, decrease in renal blood flow and claudin-2 expression besides of necrosis and apoptosis of tubular cells on 24 h. Oxidative stress was determined by measuring the oxidation of lipids and proteins and diminution in renal Nrf2 levels. Studies were also conducted in renal epithelial LLC-PK1 cells and in mitochondria isolated from kidneys of all the experimental groups. Maleate induced cell damage and reactive oxygen species (ROS) production in LLC-PK1 cells in culture. In addition, maleate treatment reduced oxygen consumption in ADP-stimulated mitochondria and diminished respiratory control index when using malate/glutamate as substrate. The activities of both complex I and aconitase were also diminished. All the above-described alterations were prevented by curcumin. It is concluded that curcumin is able to attenuate in vivo maleate-induced nephropathy and in vitro cell damage. The in vivo protection was associated to the prevention of oxidative stress and preservation of mitochondrial oxygen consumption and activity of respiratory complex I, and the in vitro protection was associated to the prevention of ROS production.
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Curcumina/farmacología , Complejo I de Transporte de Electrón/metabolismo , Hemodinámica/efectos de los fármacos , Enfermedades Renales/prevención & control , Mitocondrias/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Consumo de Oxígeno/efectos de los fármacos , Aldehído Reductasa/antagonistas & inhibidores , Animales , Antiinflamatorios no Esteroideos/farmacología , Apoptosis/efectos de los fármacos , Biomarcadores/análisis , Western Blotting , Complejo I de Transporte de Electrón/efectos de los fármacos , Inhibidores Enzimáticos/toxicidad , Enfermedades Renales/inducido químicamente , Células LLC-PK1 , Peroxidación de Lípido/efectos de los fármacos , Masculino , Maleatos/toxicidad , Mitocondrias/metabolismo , Oxidación-Reducción , Ratas , Ratas Wistar , Especies Reactivas de Oxígeno/metabolismo , PorcinosRESUMEN
BACKGROUND: Airborne Plantago pollen triggers respiratory allergies in Mediterranean countries. OBJECTIVES: We aimed to study sensitization in patients with seasonal respiratory allergy and identify proteins of Plantago lanceolata pollen that could be responsible for hypersensitivity reactions in sensitized patients. We also determined the airborne pollen concentration of Plantago species from 2004 to 2011. METHODS: IgE-binding proteins were analyzed and characterized using 1D and 2D gel electrophoresis and immunoblotting with sera from individuals sensitized to P lanceolata pollen extracts, mass spectrometry analysis, and protein data mining. We used aerobiological methods to study airborne pollen. RESULTS: P lanceolata pollen accounts for 3% of the annual pollen spectrum in the air of Porto. Of a total of 372 patients, 115 (31%) showed specific IgE levels to P lanceolata pollen extracts. All sera from P lanceolata-allergic patients recognized 8 prominent groups of IgE-reactive allergens. Separation of proteins using 2D gel electrophoresis followed by identification with mass spectrometry revealed the presence of other IgE-reactive components that could be involved in sensitization. CONCLUSIONS: We detected proteins in P lanceolata pollen extracts that, to our knowledge, have not yet been studied and could worsen sensitization to this weed pollen species. The proteins identified were involved in a variety of cellular functions. By applying 2D electrophoresis and immunoblotting with a pool of 2 sera from different P lanceolata-allergic patients, we obtained a more detailed characterization of the P lanceolata allergen profile.
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Alérgenos/análisis , Polen/inmunología , Proteómica/métodos , Electroforesis en Gel Bidimensional , Humanos , Immunoblotting , Inmunoglobulina E/sangre , Plantago/inmunología , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
Background: Airborne Plantago pollen triggers respiratory allergies in Mediterranean countries. Objectives: We aimed to study sensitization in patients with seasonal respiratory allergy and identify proteins of Plantago lanceolata pollen that could be responsible for hypersensitivity reactions in sensitized patients. We also determined the airborne pollen concentration of Plantago species from 2004 to 2011. Methods: IgE-binding proteins were analyzed and characterized using 1D and 2D gel electrophoresis and immunoblotting with sera from individuals sensitized to P lanceolata pollen extracts, mass spectrometry analysis, and protein data mining. We used aerobiological methods to study airborne pollen. Results: P lanceolata pollen accounts for 3% of the annual pollen spectrum in the air of Porto. Of a total of 372 patients, 115 (31%) showed specific IgE levels to P lanceolata pollen extracts. All sera from P lanceolata allergic patients recognized 8 prominent groups of IgE-reactive allergens. Separation of proteins using 2D gel electrophoresis followed by identification with mass spectrometry revealed the presence of other IgE-reactive components that could be involved in sensitization. Conclusions: We detected proteins in P lanceolata pollen extracts that, to our knowledge, have not yet been studied and could worsen sensitization to this weed pollen species. The proteins identified were involved in a variety of cellular functions. By applying 2D electrophoresis and immunoblotting with a pool of 2 sera from different P lanceolata -allergic patients, we obtained a more detailed characterization of the P lanceolata allergen profile (AU)
Antecedentes: El polen de Plantago provoca alergia respiratoria en los países mediterráneos. Objetivos: El objetivo de este estudio fue analizar las sensibilizaciones de pacientes con alergia estacional e identificar las proteínas de polen de Plantago lanceolata que puedan ser responsables de las reacciones de hipersensibilidad en pacientes sensibles. Adicionalmente determinamos la concentración de polen de Plantago spp en el aire, en los años 2004-2011. Métodos: Las proteínas que se unen a la IgE fueron analizadas y caracterizadas a través de electroforesis en gel 1-D y 2-D e inmunobloting con suero de pacientes sensibilizados al polen de P. lanceolata . Se analizó mediante espectrometría de masas el contenido en las proteínas y se aplicaron métodos aerobiológicos para estudiar el espectro de polen en el ambiente. Resultados: En cuanto a los resultados obtenidos, el polen de P. lanceolata representa el 3% del espectro de polen anual en la atmósfera de Oporto. De los 372 pacientes, el 31% presentaban IgE específica frente al polen de P. lanceolata. Todos los sueros de los pacientes alérgicos a P. Lanceolata reconocían los ocho grupos prominentes de alérgenos reactivos a IgE. La separación de proteínas mediante electroforesis en gel 2-D, seguida de la espectrofotometría de masas permitieron identificar en el polen la presencia de otros componentes IgE reactivos que podrían estar implicados en la sensibilización de estos pacientes. Conclusiones: En conclusión, este estudio muestra la presencia de proteínas en el polen de P. Lanceolata que hasta ahora no habían sido estudiadas y que pueden intervenir en la sensibilización a éste polen. Se detectaron proteínas involucradas en una gran variedad de funciones celulares. Mediante las técnicas aplicadas en este estudio, entre ellas el inmunobloting, nos permite realizar una detallada caracterización del perfil alergénico del polen de P. lanceolata (AU)
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Humanos , Masculino , Femenino , Alérgenos/análisis , Polen/inmunología , Proteómica/métodos , Inmunoglobulina E/administración & dosificación , Inmunoglobulina E/análisis , Plantago/química , Plantago/inmunología , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/instrumentación , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Electroforesis en Gel Bidimensional/métodos , Electroforesis en Gel Bidimensional/tendencias , Electroforesis en Gel Bidimensional , ImmunoblottingRESUMEN
Culex (Culex) pipiens (Diptera: Culicidae) has two recognized biotypes, pipiens and molestus, which differ in physiology and behaviour; this difference may influence vectorial capacity for West Nile virus (WNV). Our goal was first to determine the presence of Cx. pipiens populations in 31 locations in Portugal and to subsequently analyse their host-feeding preferences and habitat determinants. Molecular identification of Cx. pipiens forms and their hybrids was performed in 97 females; bloodmeal sources were identified in 59 engorged specimens. Overall, 61.9% of specimens were identified as Cx. pipiens f. pipiens, 20.6% as Cx. pipiens f. molestus, and 17.5% as hybrid forms. Culex pipiens f. pipiens fed preferentially on birds, and Cx. pipiens f. molestus on humans. Hybrid forms fed mostly on birds, but human bloodmeals were common. With reference to habitat, Cx. pipiens f. pipiens and hybrid forms were positively correlated with peri-urban habitats. Our results confirm the sympatric presence of different Cx. pipiens biotypes in 14 of the 31 locations studied. Peri-urban areas were a common habitat of all biotypes and may represent zones of hybridization. The feeding preferences and sympatric distribution of the Cx. pipiens biotypes observed in Portugal favour the epizootic circulation of WNV and the occurrence of disease outbreaks of WNV.
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Culex/fisiología , Ecosistema , Insectos Vectores/fisiología , Simpatría , Animales , Culex/genética , Conducta Alimentaria , Femenino , Humanos , Hibridación Genética , Insectos Vectores/genética , Masculino , Repeticiones de Microsatélite , Reacción en Cadena de la Polimerasa , Polimorfismo Genético , Portugal , Especificidad de la Especie , Fiebre del Nilo Occidental/transmisión , Fiebre del Nilo Occidental/virología , Virus del Nilo Occidental/fisiologíaRESUMEN
BACKGROUND: Autoantibodies have been detected in sera before diagnosis of cancer leading to interest in their potential as screening/early detection biomarkers. As we have found autoantibodies to MUC1 glycopeptides to be elevated in early-stage breast cancer patients, in this study we analysed these autoantibodies in large population cohorts of sera taken before cancer diagnosis. METHODS: Serum samples from women who subsequently developed breast cancer, and aged-matched controls, were identified from UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) and Guernsey serum banks to formed discovery and validation sets. These were screened on a microarray platform of 60mer MUC1 glycopeptides and recombinant MUC1 containing 16 tandem repeats. Additional case-control sets comprised of women who subsequently developed ovarian, pancreatic and lung cancer were also screened on the arrays. RESULTS: In the discovery (273 cases, 273 controls) and the two validation sets (UKCTOCS 426 cases, 426 controls; Guernsey 303 cases and 606 controls), no differences were found in autoantibody reactivity to MUC1 tandem repeat peptide or glycoforms between cases and controls. Furthermore, no differences were observed between ovarian, pancreatic and lung cancer cases and controls. CONCLUSION: This robust, validated study shows autoantibodies to MUC1 peptide or glycopeptides cannot be used for breast, ovarian, lung or pancreatic cancer screening. This has significant implications for research on the use of MUC1 in cancer detection.
Asunto(s)
Autoanticuerpos/sangre , Neoplasias de la Mama/diagnóstico , Carcinoma/diagnóstico , Detección Precoz del Cáncer/métodos , Neoplasias Pulmonares/diagnóstico , Mucina-1/inmunología , Neoplasias Ováricas/diagnóstico , Neoplasias Pancreáticas/diagnóstico , Adulto , Anciano , Neoplasias de la Mama/sangre , Neoplasias de la Mama/inmunología , Carcinoma/sangre , Carcinoma/inmunología , Estudios de Casos y Controles , Estudios de Cohortes , Femenino , Glicopéptidos/inmunología , Humanos , Inmunoensayo , Neoplasias Pulmonares/sangre , Neoplasias Pulmonares/inmunología , Persona de Mediana Edad , Neoplasias Ováricas/sangre , Neoplasias Ováricas/inmunología , Neoplasias Pancreáticas/sangre , Neoplasias Pancreáticas/inmunologíaRESUMEN
An outbreak of dengue fever in Madeira island was reported in 2012. Clinical and laboratory findings of the first two laboratory-confirmed autochthonous cases are reported. Both cases had fever (≥38 °C) and petechial rash. Symptoms also included myalgia, asthenia, nausea, vomiting, anorexia, diffuse abdominal pain, and diarrhoea. The two cases were confirmed by serology and one tested positive for a dengue viral sequence. Dengue virus serotype DEN-1 was identified with probable Central or South American origin.
Asunto(s)
Virus del Dengue/genética , Virus del Dengue/aislamiento & purificación , Dengue/diagnóstico , Dengue/epidemiología , Brotes de Enfermedades , Adolescente , Adulto , Aedes/virología , Animales , Técnicas de Laboratorio Clínico , Dengue/transmisión , Dengue/virología , Virus del Dengue/clasificación , Femenino , Humanos , Masculino , Filogenia , Portugal/epidemiología , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia , SerotipificaciónRESUMEN
The subversion of the normal function exerted by the cellular prion protein (PrP(C)) in neurons by pathogenic prions is assumed to have a central role in the pathogenesis of transmissible spongiform encephalopathies. Using two murine models of prion infection, the 1C11 neuronal cell line and neurospheres, we document that prion infection is associated with the constitutive activation of signaling targets normally coupled with PrP(C), including the Fyn kinase, the mitogen-associated protein kinases ERK1/2 and the CREB transcription factor. PrP(C)-dependent signaling overactivation in infected cells is associated with the recruitment of p38 and JNK stress-associated kinases. Downstream from CREB, prion-infected cells exhibit reduced activity of the matrix metalloprotease (MMP)-9. As MMP-9 catalyzes the degradation of the amyloid A-beta peptide, the decrease in MMP-9 activity in prion-infected cells causes a significant impairment of the clearance of A-beta, leading to its accumulation. By exploiting two 1C11-infected clones accumulating high or moderate levels of prions, we show that the prion-induced changes are correlated with the level of infectivity. Of note, a dose-dependent increase in A-beta levels was also found in the cerebrospinal fluid of mice inoculated with these infected clones. By demonstrating that pathogenic prions trigger increases in A-beta levels through the deviation of PrP(C) signaling, our data argue that A-beta may exacerbate prion-induced toxicity.
Asunto(s)
Péptidos beta-Amiloides/metabolismo , Neuronas/metabolismo , Proteínas PrPC/metabolismo , Priones/metabolismo , Péptidos beta-Amiloides/líquido cefalorraquídeo , Animales , Línea Celular , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Proteína 1 de la Respuesta de Crecimiento Precoz/metabolismo , Proteínas Quinasas JNK Activadas por Mitógenos/metabolismo , Metaloproteinasa 9 de la Matriz/metabolismo , Ratones , Proteína Quinasa 1 Activada por Mitógenos/metabolismo , Proteína Quinasa 3 Activada por Mitógenos/metabolismo , Neuronas/citología , Fosforilación , Enfermedades por Prión/metabolismo , Enfermedades por Prión/patología , Proteínas Proto-Oncogénicas c-fyn/antagonistas & inhibidores , Proteínas Proto-Oncogénicas c-fyn/genética , Proteínas Proto-Oncogénicas c-fyn/metabolismo , Interferencia de ARN , ARN Interferente Pequeño/metabolismo , Transducción de Señal , Proteínas Quinasas p38 Activadas por Mitógenos/metabolismoRESUMEN
Thrombus formation, due to thrombin generation, is a major problem affecting blood-contacting medical devices. This work aimed to develop a new strategy to improve the hemocompatibility of such devices by the immobilization of a naturally occurring thrombin inhibitor into a nanostructured surface. Boophilin, a direct thrombin inhibitor from the cattle tick Rhipicephalus microplus, was produced as a recombinant protein in Pichia pastoris. Boophilin was biotinylated and immobilized on biotin-terminated self-assembled monolayers (SAM) via neutravidin. In order to maintain its proteinase inhibitory capacity after surface immobilization, boophilin was biotinylated after the formation of a boophilin-thrombin complex to minimize the biotinylation of the residues involved in thrombin-boophilin interaction. The extent of boophilin biotinylation was determined using matrix-assisted laser desorption/ionization-time of flight/time of flight mass spectrometry. Boophilin immobilization and thrombin adsorption were quantified using quartz crystal microbalance with dissipation. Thrombin competitive adsorption from human serum was assessed using ¹²5I-thrombin. Thrombin inhibition and plasma clotting time were determined using spectrophotometric techniques. Boophilin-coated SAM were able to promote thrombin adsorption in a selective way, inhibiting most of its activity and delaying plasma coagulation in comparison with boophilin-free surfaces, demonstrating boophilin's potential to improve the hemocompatibility of biomaterials used in the production of blood-contacting devices.