RESUMEN
Salmonella enterica serovar Choleraesuis (S. Choleraesuis) isolates derived from diseased pigs in Japan during 2001 and 2005 were analyzed for biotype, based on H(2)S production and dulcitol fermentation, pulsed-field gel electrophoresis (PFGE) profile, and antimicrobial resistance profile. S. Choleraesuis biotype Choleraesuis (biotype Choleraesuis) was classified into one genotype, while varietas Kunzendorf (var. Kunzendorf) was classified into two genotypes. The isolates of var. Kunzendorf belonging to one genotype were isolated in a limited area of Japan. Variation in the antimicrobial resistance pattern was observed in isolates of both biotypes Choleraesuis and var. Kunzendorf. We have also shown that the PFGE profile was associated with the biotype and isolation region of each isolate.
Asunto(s)
Salmonelosis Animal , Salmonella enterica/clasificación , Salmonella enterica/efectos de los fármacos , Animales , Antibacterianos/farmacología , Técnicas de Tipificación Bacteriana , Electroforesis en Gel de Campo Pulsado , Fermentación , Microbiología de Alimentos , Galactitol/metabolismo , Variación Genética , Genoma Bacteriano , Japón/epidemiología , Carne/microbiología , Pruebas de Sensibilidad Microbiana , Salmonelosis Animal/epidemiología , Salmonelosis Animal/microbiología , Salmonella enterica/aislamiento & purificación , Salmonella enterica/fisiología , Sulfitos/metabolismo , PorcinosRESUMEN
To determine the prevalence of the virulence plasmid in Salmonella Typhimurium isolates from pigs in Japan, a total of 106 porcine isolates were subjected to PCR amplification for the detection of the virulence plasmid. Out of the isolates of S. Typhimurium, 38 (35.8%) harbored the virulence plasmid. The presence of the virulence plasmid was widely observed in the isolates from systemically infected pigs (92.0%, 23/25), compared with diarrheic (18.8%, 12/64) and apparently healthy pigs (17.6%, 3/17) (P<0.01).
Asunto(s)
Plásmidos/genética , Salmonelosis Animal/microbiología , Salmonella typhimurium/genética , Enfermedades de los Porcinos/microbiología , Animales , ADN Bacteriano/química , ADN Bacteriano/genética , Femenino , Reacción en Cadena de la Polimerasa/veterinaria , Salmonella typhimurium/aislamiento & purificación , Salmonella typhimurium/patogenicidad , Porcinos , VirulenciaRESUMEN
The growth of Salmonella Choleraesuis was examined in Rappaport Vassiliadis broth (RV) and Hajna-tetrathionate broth (HTT) at 37 and 42 degrees C. As the enrichment in RV at 37 degrees C was satisfactory for isolating S. Choleraesuis, we used this enrichment for isolation from the samples collected from 15 asymptomatic pigs reared on a S. Choleraesuis contaminated farm. S. Choleraesuis was frequently isolated from six pigs (40.0%) under field conditions. The isolation of other Salmonella serovars than S. Choleraesuis was attempted by using both RV enrichment at 37 degrees C and HTT enrichment at 42 degrees C. Salmonella organisms were isolated from 156 (44.8%) of 348 fecal samples and more frequently with HTT at 42 degrees C (43.4%) than with RV at 37 degrees C (20.9%). If other serovars in addition to S. Choleraesuis are to be surveyed, HTT enrichment should be used in combination with RV enrichment.
Asunto(s)
Salmonelosis Animal/microbiología , Salmonella/aislamiento & purificación , Enfermedades de los Porcinos/microbiología , Porcinos/microbiología , Animales , Medios de Cultivo , Geografía , Salmonella/clasificación , Salmonella/crecimiento & desarrollo , Especificidad de la EspecieRESUMEN
A total of 267 fecal and serum samples collected from individual pigs reared on a Salmonella-positive farm were subjected to bacteriological and serological examinations of Salmonella. Salmonella was isolated from 47 pigs (17.6%) and prevalence of antibody to lipopolysaccharide (LPS) of S. Typhimurium, which was partly common to S. O4, 12: d: -, was observed in 90 pigs (33.7%). Salmonella was isolated from 26 (28.9%) of 90 antibody-positive pigs and 21 (11.9%) of 177 antibody-negative pigs. Twenty-one of 36 pigs (58.3%) positive for S. O4, 12: d: -, five of 10 pigs (50.0%) positive for S. Havana, and none for S. Anatum had antibodies. Thus, seropositive rates were higher than isolation-positive rates, and antibody prevalence was associated with serovars of the isolates. Then, we analyzed antibody prevalence among pigs on Japanese pig farms. The antibodies to LPS of S. Typhimurium were found in 195 of 1,498 pigs (13.0%) and in at least one serum sample on 35 of 52 farms (67.3%). Our results indicate that Salmonella does not seem to be so prevalent in pigs though it is widely prevalent among pig farms.
Asunto(s)
Salmonella/aislamiento & purificación , Porcinos/microbiología , Envejecimiento , Animales , Anticuerpos Antibacterianos/sangre , Heces/microbiología , Japón , Prevalencia , Salmonella/clasificación , Salmonella/inmunología , Estudios Seroepidemiológicos , Porcinos/inmunologíaRESUMEN
A survey of Salmonella was carried out in fecal samples of 887 pigs with diarrhea collected from 235 pig farms between April 1996 and March 2001. Salmonella was isolated from 84 feces (9.5%) of 887 pigs and from 45 (19.1%) of 235 farms. The higher prevalence was found in weaned pigs (12.4%) and fattening pigs (17.3%) than in sows (4.2%) and suckling pigs (4.5%). Isolation rates of S. Typhimurium were higher from weaned and fattening pigs than from the others. Therefore, risk of horizontal infection of S. Typhimurium will increase, if no adequate health managements are practiced when weaned and fattening pigs have diarrhea.