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Biocontrol Sci ; 24(4): 221-227, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31875614

RESUMEN

The commercially available 3 types of selective media in Japan were compared for the detection of Bacillus cereus. When assessed inclusivity using 25 B. cereus strains, MYP agar, NGKG agar, and chromogenic X-BC agar demonstrated excellent inclusivity. For exclusivity study using 50 non-B. cereus strains, MYP, NGKG, and X-BC allowed to grow 11, 7, and 3 strains, respectively. Of the grown bacteria on each strains tested, only 2 strains of B. thuringiensis formed typical B. cereus colonies on all selective media tested. The NGKG and X-BC were compared with MYP as a reference using artificially contaminated food (fried rice, plain rice, fried noodle, and potato salad ), since MYP is recommended in ISO 7932: 2004. The both correlation coefficients between NGKG and MYP, and X-BC and MYP were 0.999. Therefore, we demonstrated that NGKG and X-BC can be adapted to ISO 7932: 2004 method for selected food as well as MYP.


Asunto(s)
Bacillus cereus/aislamiento & purificación , Medios de Cultivo , Microbiología de Alimentos , Agar , Bacillus cereus/crecimiento & desarrollo , Bacillus thuringiensis/crecimiento & desarrollo , Bacillus thuringiensis/aislamiento & purificación , Carga Bacteriana , Medios de Cultivo/química , Contaminación de Alimentos , Microbiología de Alimentos/normas , Inocuidad de los Alimentos , Humanos , Japón
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