1.
Chem Commun (Camb)
; 53(91): 12274-12277, 2017 Nov 14.
Artículo
en Inglés
| MEDLINE
| ID: mdl-29090689
RESUMEN
Biocatalysis is a fast developing field in which an enzyme's natural capabilities are harnessed or engineered for synthetic chemistry. The enzyme PatG is an extremely promiscuous macrocyclase enzyme tolerating both non-natural amino acids and non-amino acids within the substrate. It does, however, require a proline or thiazoline at the C-terminal position of the core peptide which means the final product must contain this group. Here, we show guided by structural insight we have identified two synthetic routes, triazole and a double cysteine, that circumvent this requirement. With the triazole, we show PatGmac can macrocyclise substrates that do not contain any amino acids in the final product.
Asunto(s)
Ligasas/metabolismo , Prolina/metabolismo , Tiazoles/metabolismo , Biocatálisis , Prolina/química , Especificidad por Sustrato , Tiazoles/química
2.
Clin Nephrol
; 50(3): 198, 1998 Sep.
Artículo
en Inglés
| MEDLINE
| ID: mdl-9776427