[BONE METABOLISM AND BONE MINERAL DENSITY IN CHILDREN WITH RECURRENT BRONCHITIS].

The aim - to determine the bone metabolism status and bone mineral density (BMD) in children with recurrent bronchitis (RB). 120 children aged 6 to 10 years were examined. Formed 2 groups: 1st group - children with RB (n=60); 2nd group - children occasionally suffering of upper respiratory tract infections (n=60). The average age was 8 years 2 months. Among children with RB, compared with children of the 2nd group, an increase in the number of patients with an increase in alkaline phosphatase activity (by 35.0%) (p<0.05), and serum osteocalcin level (by 66.7%) (p<0.05) was revealed, collagen type 1 degradation products (by 15.0%) (p<0.05), parathyroid hormone (by 13.3%) (p<0.05), decrease of serum 25(OH)D less than 30 ng/ml (by 59.3%) (p<0.05). A DXA in children with RB showed the presence of 24 (40.0%) episodes of decreased BMD in the L1, 25 (41.7%) episodes in L2, 24 (40.0%) episodes in L3, 27 (45.0%) episodes in L4, 28 (46.7%) cases in the left femur neck, 22 (36.7%) cases in the right femur neck. In children with RB was established activation of bone resorption on background of vitamin D deficiency and BMD decrease.

[SERUM CONTENTS OF SUBSTANCE P, ENDOTHELIN-1 AND VASOACTIVE INTESTINAL PEPTIDE IN CHILDREN WITH RECURRENT BRONCHITIS AND VERTEBROBASILAR INSUFFICIENCY].

The recurrent bronchitis (RB) course is caused by the bronchi secretory-evacuation mechanisms state, which provide clearance from pathogens. This mechanism can be disrupted by vegetative reflexes and neuropeptides imbalance that develops in children with the syndrome of the vertebrobasilar arterial system (SVBAS). The objective: study of the neurogenic maintenance of the RB pathogenesis in children with SVBAS by studying the serum content of substances affecting of the bronchial mucosa secretory-evacuation function and inflammatory activity (substance P, vasoactive intestinal peptide - VIP and endothelin-1 - ET-1). 90 children aged 7 to 11 years were examined, 3 observation groups were formed: Group 1 - children with RB and SVBAS (n=30); Group 2 - children with SVBAS without RB (n=30); Group 3 - children with RB without SVBAS (n=30). In the Group 1, compared with the 2nd and 3rd, there was an increase in the children number with high serum content of substance P (by 66.7% and 50.0%, respectively, p<0.05) and ET -1 (by 23.3% and 40.0%, respectively, p<0.05), low content of VIP (by 46.7% and 23.4%, respectively, p<0.05). Children with RB and SVBAS have serum level imbalance of the pro-inflammatory substance P, ET-1 and anti-inflammatory VIP as the bronchitis severe course basis.

[The vitamin D sufficiency in children with recurrent bronchitis].

UNLABELLED: Currently in the world there is no consensus on the provision of sufficient vitamin D and its optimum serum levels of both healthy children and patients with various pathological conditions. THE OBJECTIVE: investigation of vitamin D sufficiency in children with recurrent bronchitis (RB), living in Zaporozhye, by examining of 25(OH)D and parathormone serum level. The study involved 120 children aged 4 to 10 years, divided into 2 groups (60 children each): 1) children, occasionally ill with acute respiratory infections, 2) children with RB. Investigation of serum 25(OH)D was conducted between November and February. Decrease of vitamin D3 below 30 ng/ml in serum was observed in 85% (р<0,05) patients with RB (insufficiency), below 20 ng/ml - in 15% (deficit). The children aged 4-10 years with RB, who living in Zaporozhye, have decrease of serum 25(OH)D that characterizes their vitamin D3 supply as insufficient.

Methylobacterium sp. resides in unculturable state in potato tissues in vitro and becomes culturable after induction by Pseudomonas fluorescens IMGB163.

AIMS: To induce growth of endophytic bacteria residing in an unculturable state in tissues of in vitro-grown potato plantlets. To isolate and identify the induced bacteria and to localize the strains in tissues of in vitro-grown potato plantlets. METHODS AND RESULTS: The inoculation of in vitro-grown potato plants with Pseudomonas fluorescens IMBG163 led to induction of another bacterium, a pink-pigmented facultative methylotroph that was identified as Methylobacterium sp. using phylogenetic 16S rDNA approach. Two molecular methods were used for localizing methylobacteria in potato plantlets: PCR and in situ hybridization (ISH/FISH). A PCR product specific for the Methylobacterium genus was found in DNA isolated from the surface-sterilized plantlet leaves. Presence of Methylobacterium rRNA was detected by ISH/FISH in leaves and stems of inoculated as well as axenic potato plantlets although the bacterium cannot be isolated from the axenic plants. CONCLUSION: Methylobacterium sp. resides in unculturable state within tissues of in vitro-grown potato plants and becomes culturable after inoculation with P. fluorescens IMBG163. SIGNIFICANCE AND IMPACT OF THE STUDY: In order to develop endophytic biofertilizers and biocontrol agents, a detailed knowledge of the life-style of endophytes is essential. To our knowledge, this is the first report on increase of the culturability of endophytes in response to inoculation by nonpathogenic bacteria.

[Diagnosis and therapy of tumors and tumor-like formations of the stomach and intestine in children]. / Diagnostika i lechenie opukholei i opukholepodobnykh obrazovanii zheludka i kishechnika u detei.

The experience with diagnosis and treatment of 45 children at the age of from 2 mos to 15 yrs with tumours and tumour-like formations of the stomach and intestine was summarized. Most frequently (more than in 60% of cases), lymphosarcoma was revealed, cancer--in 4.4% of cases. The main causes of diagnostic errors--ignoring the data of history, wrong interpretation of the results of palpation of the abdomen, incomplete examination of a patient and absence of oncologic suspiciousness in a physician. Results of treatment of the patients with malignant masses depended on a stage of the disease.

[Characteristics of the endocrine and immune systems and lipid metabolism in children with lymphatico-hyperplastic diathesis]. / Osobennosti éndokrinnoi, immunnoi sistem i zhirovogo obmena u detei s limfatiko-giperplasticheskim diatezom.

The data on the genealogic anamnesis and of the incidence of lymphohyperplastic diathesis (LHD) are presented. The measurements of the size of the thymus depending on the age were made with account of the thymicothoracic index. Substantial alterations were discovered in the function of the endocrine system (high content of somatotropic hormone, reduced content of ACTH, hydrocortisone, and TTH), a decline of the relative amount of T and B lymphocytes, and persistent hypoimmunoglobulinemia (G, M, A). The high content of total lipids, cholesterol, triglycerides, low density and very low density lipoproteins and the reduced content of high density lipoproteins were detectable since the first months of life, persisting, up to 3 years of life. Children with LHD should be registered in the dispensary second health group since the first months of life.

[Pathogenetic mechanisms of the development of cardiac rhythm disorders in the acute period of myocardial infarction]. / Patogeneticheskie mekhanizmy razvitiia narushenii ritma serdtsa v ostrom periode infarkta miokarda.

The clinical course and biochemical, radioimmune and electrophysiologic findings were assessed in 421 patients with acute myocardial infarction involving heart rhythm and conductivity disorders and 49 patients with acute myocardial infarction, free of cardiac electrical instability. The studies were carried out during arrhythmias and after they were controlled, prior to treatment, at the peak of antiarrhythmic effect of medication, and after a continuous treatment course. Heart rhythm disorders related to acute myocardial infarction were accompanied with a marked response on the part of blood free fatty acids, sympatho-adrenal and kallikrein-kinin activity and carboxycathepsin. Metabolic and neurohumoral changes were eventually reversed and tended to normalize as soon as the sinus rhythm was restored by treatment.