Locomotor Behaviour and Clock Neurons Organisation in the Agricultural Pest Drosophila suzukii.

Drosophila suzukii (Matsumara) also called Spotted Wing Drosophila (SWD), is an invasive pest species originally from Asia that has now spread widely across Europe and North America. The majority of drosophilids including the best known Drosophila melanogaster only breed on decaying fruits. On the contrary, the presence of a strong serrated ovipositor and behavioural and metabolic adaptations allow D. suzukii to lay eggs inside healthy, ripening fruits that are still on the plant. Here we present an analysis of the rhythmic locomotor activity behaviour of D. suzukii under several laboratory settings. Moreover, we identify the canonical clock neurons in this species by reporting the expression pattern of the major clock proteins in the brain. Interestingly, a fundamentally similar organisation of the clock neurons network between D. melanogaster and D. suzukii does not correspond to similar characteristics in rhythmic locomotor activity behaviour.

The Euphausia superba transcriptome database, SuperbaSE: An online, open resource for researchers.

Antarctic krill (Euphausia superba) is a crucial component of the Southern Ocean ecosystem, acting as the major link between primary production and higher trophic levels with an annual predator demand of up to 470 million tonnes. It also acts as an ecosystem engineer, affecting carbon sequestration and recycling iron and nitrogen, and has increasing importance as a commercial product in the aquaculture and health industries. Here we describe the creation of a de novo assembled head transcriptome for E. superba. As an example of its potential as a molecular resource, we relate its exploitation in identifying and characterizing numerous genes related to the circadian clock in E. superba, including the major components of the central feedback loop. We have made the transcriptome openly accessible for a wider audience of ecologists, molecular biologists, evolutionary geneticists, and others in a user-friendly format at SuperbaSE, hosted at http://www.krill.le.ac.uk.

The logic of circadian organization in Drosophila.

BACKGROUND: In the fruit fly Drosophila melanogaster, interlocked negative transcription/translation feedback loops provide the core of the circadian clock that generates rhythmic phenotypes. Although the current molecular model portrays the oscillator as cell autonomous, cross-talk among clock neurons is essential for robust cycling behavior. Nevertheless, the functional organization of the neuronal network remains obscure. RESULTS: Here we show that shortening or lengthening of the circadian period of locomotor activity can be obtained either by targeting different groups of clock cells with the same genetic manipulation or by challenging the same group of cells with activators and repressors of neuronal excitability. CONCLUSIONS: Based on these observations we interpret circadian rhythmicity as an emerging property of the circadian network and we propose an initial model for its architectural design.

The circadian clock of the fly: a neurogenetics journey through time.

Forty years ago, a mutagenesis screening in the fruit fly, Drosophila melanogaster, led to the discovery of period, the first gene to be involved in the endogenous 24-h rhythmicity of an organism. Since then circadian clocks have been identified in fungi, cyanobacteria, plants, and other animals. Although the molecular components are not conserved across the main divisions of life, it appears that in every organism, a common design, based upon a transcription-translation feedback loop (TTL), is in place to regulate endogenous 24 h cycles. The TTL model has informed chronobiology research for the majority of the past 30 years with spectacular results. However, new evidence and the rediscovery of old observations suggest that this model is coming to age. Here, we provide a comprehensive review of the current TTL model in Drosophila highlighting its accomplishments and its limitations. We conclude by offering our personal view on the organization and the evolution of circadian clocks.

Differential gene expression during the moult cycle of Antarctic krill (Euphausia superba).

BACKGROUND: All crustaceans periodically moult to renew their exoskeleton. In krill this involves partial digestion and resorption of the old exoskeleton and synthesis of new cuticle. Molecular events that underlie the moult cycle are poorly understood in calcifying crustaceans and even less so in non-calcifying organisms such as krill. To address this we constructed an Antarctic krill cDNA microarray in order to generate gene expression profiles across the moult cycle and identify possible activation pathways. RESULTS: A total of 26 different cuticle genes were identified that showed differential gene expression across the moult cycle. Almost all cuticle genes were up regulated during premoult and down regulated during late intermoult. There were a number of transcripts with significant sequence homology to genes potentially involved in the synthesis, breakdown and resorption of chitin. During early premoult glutamine synthetase, a gene involved in generating an amino acid used in the synthesis of glucosamine, a constituent of chitin, was up regulated more than twofold. Mannosyltransferase 1, a member of the glycosyltransferase family of enzymes that includes chitin synthase was also up regulated during early premoult. Transcripts homologous to a ß-N-acetylglucosaminidase (ß-NAGase) precursor were expressed at a higher level during late intermoult (prior to apolysis) than during premoult. This observation coincided with the up regulation during late intermoult, of a coatomer subunit epsilon involved in the production of vesicles that maybe used to transport the ß-NAGase precursors into the exuvial cleft. Trypsin, known to activate the ß-NAGase precursor, was up regulated more than fourfold during premoult. The up regulation of a predicted oligopeptide transporter during premoult may allow the transport of chitin breakdown products across the newly synthesised epi- and exocuticle layers. CONCLUSION: We have identified many genes differentially expressed across the moult cycle of krill that correspond with known phenotypic structural changes. This study has provided a better understanding of the processes involved in krill moulting and how they may be controlled at the gene expression level.