Mercury concentrations and stable isotopes (δ15N and δ13C) in fish muscle indicate human impacts in tropical coastal lagoons.

Coastal lagoons are essential nursery habitats of many marine fishes, but they are often sites of intense human activities that impact water quality and potentially the fish using these habitats. We compared the variability in nutrient uptake (using δ15N and δ13C) and total mercury (THg) levels in juveniles and adults of three common species in two lagoons on the central Mexican coast of the Pacific Ocean during the wet and dry seasons. One of three species, Achirus mazatlanus had higher THg, δ15N, and δ13C levels in the lagoon with the greatest wastewater inputs (Barra de Navidad). Delta13C varied seasonally for all three species and THg was higher in the dry season for Lutjanus argentiventris and in males of A. mazatlanus. Our results demonstrate that mercury and stable isotopes can identify impacts of human activities on estuarine ichthyofauna and the importance of understanding seasonal and spatial variability of measures that could impact monitoring and predictions of impacts in these lagoons.

Increased mercury and body size and changes in trophic structure of Gambusia puncticulata (Poeciliidae) along the Almendares River, Cuba.

The Almendares River is the largest river draining the area around Havana City, Cuba. The watershed is heavily populated and industrialized, which has had a significant impact on the flow and water quality of the river. The main goal of this study was to analyze the spatial variability in dietary habits, nutrient flow (using stable isotope ratios δ(15)N and δ(13)C), and mercury (Hg) levels along the Almendares River upstream and downstream of point-source discharges using localized fish Gambusia puncticulata. Stomach contents of G. puncticulata were similar among these sites. However, mean δ(15)N values ranged from 6 to 18 ‰ across sites and were lower in fish from downstream than upstream sites, suggesting localized influences of nutrient inputs along the river. δ(13)C values were between -22 and -25 ‰, except at a mid-basin site (-26 to -27‰), indicating that fish relied on similar carbon sources at most sites. Total mercury concentrations ranged from 0.04 to 0.49 µg/g wet weight whole body and were unrelated to the among-site differences in δ(15)N, but Hg exceeded the threshold considered to be protective of fish health (0.2 µg/g ww whole body) in the majority of fish from all sites but one. Results of this study indicate that although the dietary habits of this species do not vary across sites, tissue differences in δ(15)N, δ(13)C and Hg show little movement of this species among sites. Localized effects of human activities on nutrients and metals may be affecting the health of this species and posing a risk to other consumers in the ecosystem.