Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
Más filtros











Base de datos
Intervalo de año de publicación
1.
Sci Rep ; 9(1): 3357, 2019 03 04.
Artículo en Inglés | MEDLINE | ID: mdl-30833597

RESUMEN

Inherited hearing loss is associated with gene mutations that result in sensory hair cell (HC) malfunction. HC structure is defined by the cytoskeleton, which is mainly composed of actin filaments and actin-binding partners. LIM motif-containing protein kinases (LIMKs) are the primary regulators of actin dynamics and consist of two members: LIMK1 and LIMK2. Actin arrangement is directly involved in the regulation of cytoskeletal structure and the maturation of synapses in the central nervous system, and LIMKs are involved in structural plasticity by controlling the activation of the actin depolymerization protein cofilin in the olfactory system and in the hippocampus. However, the expression pattern and the role of LIMKs in mouse cochlear development and synapse function also need to be further studied. We show here that the Limk genes are expressed in the mouse cochlea. We examined the morphology and the afferent synapse densities of HCs and measured the auditory function in Limk1 and Limk2 double knockout (DKO) mice. We found that the loss of Limk1 and Limk2 did not appear to affect the overall development of the cochlea, including the number of HCs and the structure of hair bundles. There were no significant differences in auditory thresholds between DKO mice and wild-type littermates. However, the expression of p-cofilin in the DKO mice was significantly decreased. Additionally, no significant differences were found in the number or distribution of ribbon synapses between the DKO and wild-type mice. In summary, our data suggest that the Limk genes play a different role in the development of the cochlea compared to their role in the central nervous system.


Asunto(s)
Cóclea/crecimiento & desarrollo , Audición/fisiología , Quinasas Lim/genética , Factores Despolimerizantes de la Actina/metabolismo , Actinas/metabolismo , Animales , Células Ciliadas Auditivas , Quinasas Lim/deficiencia , Quinasas Lim/fisiología , Ratones , Ratones Noqueados , Sinapsis
2.
Front Mol Neurosci ; 11: 183, 2018.
Artículo en Inglés | MEDLINE | ID: mdl-29904342

RESUMEN

In developing sensory systems, elaborate morphological connectivity between peripheral cells and first-order central neurons emerges via genetic programming before the onset of sensory activities. However, how the first-order central neurons acquire the capacity to interface with peripheral cells remains elusive. By making patch-clamp recordings from mouse brainstem slices, we found that a subset of neurons in the cochlear nuclei, the first central station to receive peripheral acoustic impulses, exhibits spontaneous firings (SFs) as early as at birth, and the fraction of such neurons increases during the prehearing period. SFs are reduced but not eliminated by a cocktail of blockers for excitatory and inhibitory synaptic inputs, implicating the involvement of intrinsic pacemaker channels. Furthermore, we demonstrate that these intrinsic firings (IFs) are largely driven by hyperpolarization- and cyclic nucleotide-gated channel (HCN) mediated currents (Ih), as evidenced by their attenuation in the presence of HCN blockers or in neurons from HCN1 knockout mice. Interestingly, genetic deletion of HCN1 cannot be fully compensated by other pacemaker conductances and precludes age-dependent up regulation in the fraction of spontaneous active neurons and their firing rate. Surprisingly, neurons with SFs show accelerated development in excitability, spike waveform and firing pattern as well as synaptic pruning towards mature phenotypes compared to those without SFs. Our results imply that SFs of the first-order central neurons may reciprocally promote their wiring and firing with peripheral inputs, potentially enabling the correlated activity and crosstalk between the developing brain and external environment.

3.
Sci Rep ; 7(1): 6409, 2017 07 25.
Artículo en Inglés | MEDLINE | ID: mdl-28743950

RESUMEN

Auditory neuropathy (AN) is a hearing disorder characterized by normal cochlear amplification to sound but poor temporal processing and auditory perception in noisy backgrounds. These deficits likely result from impairments in auditory neural synchrony; such dyssynchrony of the neural responses has been linked to demyelination of auditory nerve fibers. However, no appropriate animal models are currently available that mimic this pathology. In this study, Cre-inducible diphtheria toxin receptor (iDTR +/+ ) mice were cross-mated with mice containing Cre (Bhlhb5-Cre +/- ) specific to spiral ganglion neurons (SGNs). In double-positive offspring mice, the injection of diphtheria toxin (DT) led to a 30-40% rate of death for SGNs, but no hair cell damage. Demyelination types of pathologies were observed around the surviving SGNs and their fibers, many of which were distorted in shape. Correspondingly, a significant reduction in response synchrony to amplitude modulation was observed in this group of animals compared to the controls, which had a Cre- genotype. Taken together, our results suggest that SGN damage following the injection of DT in mice with Bhlhb5-Cre +/- and iDTR +/- is likely to be a good AN model of demyelination.


Asunto(s)
Toxina Diftérica/toxicidad , Pérdida Auditiva Central/fisiopatología , Factor de Crecimiento Similar a EGF de Unión a Heparina/metabolismo , Ganglio Espiral de la Cóclea/patología , Animales , Cóclea/fisiopatología , Modelos Animales de Enfermedad , Potenciales Evocados Auditivos del Tronco Encefálico/efectos de los fármacos , Femenino , Masculino , Ratones Mutantes , Ratones Transgénicos , Ganglio Espiral de la Cóclea/efectos de los fármacos , Ganglio Espiral de la Cóclea/metabolismo
4.
Artículo en Chino | MEDLINE | ID: mdl-27192923

RESUMEN

Cochlear implantation has been a standard therapy for treating severe deafness because patients who receive it have better speech perception. However, the hearing performance of cochlear implantation in noisy environment is far from satisfaction. Efforts have been made to reverse such condition, such as EAS, bimodal stimulation, environment-adaptive speech enhancement and multipolar stimulation, and patients who receive it get more or less better speech perception in noisy environment than traditional cochlear implantation.


Asunto(s)
Implantes Cocleares , Ruido , Percepción del Habla , Diseño de Equipo , Humanos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA