RESUMEN
The NAFLD rats were intervened with the extracts of Polygonum cuspidatum compound for 4 weeks. The reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) methods were used to detecte the relative level of leptin mRNA in the adipose tissue of intervenient and control groups. Their variances of fat and glucose in serum were detected with the biochemical methods. The results showed that the level of leptin mRNA of intervenient group was significantly increased (P <0.05) and the triglycered and total cholesterol were significantly decreased (P <0.05). The extracts of Polygonum cuspidatum compound could increase leptin mRNA level in adipose tissue and improve the fat metablolism in serum. However, the serum glucose of the intervenient group was a little raised (P>0.05).
Asunto(s)
Tejido Adiposo/efectos de los fármacos , Medicamentos Herbarios Chinos/farmacología , Hígado Graso/genética , Leptina/genética , Tejido Adiposo/metabolismo , Animales , Glucemia/metabolismo , Colesterol/sangre , Medicamentos Herbarios Chinos/aislamiento & purificación , Hígado Graso/sangre , Hígado Graso/patología , Femenino , Hipolipemiantes/farmacología , Leptina/biosíntesis , Hígado/efectos de los fármacos , Hígado/metabolismo , Hígado/patología , Masculino , ARN Mensajero/genética , ARN Mensajero/metabolismo , Distribución Aleatoria , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Triglicéridos/sangreRESUMEN
OBJECTIVE: To investigate the toxicity attenuation and efficacy potentiation effects of Fu Zheng Yang Yin Decoction with HIFU on the experimental model of VX2 liver cancer in rabbits. METHODS: The rabbits were all transplanted with VX2 cancer on livers. The 30 rabbits were divided into three groups: HIFU group (HIFU therapy), Combination group (HIFU + Fu Zheng Yang Yin decoction), Control group (without any treatment). Each group involved 10 rabbits. After treatment, the volume of the tumor was observed by sonography. Metastases, pathological changes, Immunoglobulin, ALT were also observed. RESULTS: After treatment, ALT and temperature of Combination group and HIFU group were lower than Control group (P < 0.05). The tumor volume of Combination group and HIFU group showed significant difference with control group (P < 0.05). Immunoglobulin of HIFU group and Combination group increased, that of Control group decreased. One month after treatment, all animals were dissected. Combination group had no lung transferred, HIFU group had 2 lung transferred, all of control group had lung transferred. CONCLUSION: The combination therapy of Fu Zheng Yang Yin decoction with HIFU can effectively inhibited VX2 cancer growth in rabbit's liver. Fu Zheng Yang Yin decoction can reduce side-effect, improve immune function, and has special effects of toxicity attenuation and efficacy potentiation.
Asunto(s)
Antineoplásicos Fitogénicos/uso terapéutico , Medicamentos Herbarios Chinos/uso terapéutico , Neoplasias Hepáticas Experimentales/terapia , Alanina Transaminasa/sangre , Animales , Antineoplásicos Fitogénicos/administración & dosificación , Línea Celular Tumoral , Terapia Combinada , Medicamentos Herbarios Chinos/administración & dosificación , Femenino , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Neoplasias Hepáticas Experimentales/sangre , Neoplasias Hepáticas Experimentales/patología , Masculino , Fitoterapia/métodos , Conejos , Distribución Aleatoria , Resultado del Tratamiento , Terapia por Ultrasonido/métodosRESUMEN
The real-time qPCR method had been used to detect and analyze the non-alcohol fatty liver disease (NEFLD) model in medical intervention in this research. The relative level of TNF-alpha mRNA in adipose tissue of intervention group was lower than that of control group. Their difference was significant (t = 2.452, P = 0.22). Compared with the control group, it decreased that the contents of liver trilyceride, total cholesterol, and glucose in intervention group. The difference of total cholesterol between two groups was significant (t = 2.555, P = 0.019). The extracts of Rizoma Polygoni Cuspidati could significantly decrease TNF-alpha mRNA level in adipose tissue, and it could decrease the contents of triglyceride, total cholesterol, and glucose in liver tissue. This Chinese traditional medicine can adjust the metabolism of liver adipose and glucose,and improve steatosis in liver cell.
