RESUMEN
OBJECTIVE: Oral squamous cell carcinoma (OSCC) is still one of the most frequent neck and head malignancies and is one of the most common cancers in the world. The main purpose of this research was to illustrate the functional role of LINC00961 in OSCC and provide novel insight of biomarkers and therapeutic strategies in OSCC. PATIENTS AND METHODS: The relative expression level of LINC00961 was evaluated by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay was involved for determining the ability of cell proliferation. Flow cytometric analysis was performed to detect the cell cycle and cell apoptosis. Expressions of AKT, p-AKT, BCL2, Bax protein levels were detected in Western blotting. Transfected cells were used to perform tumor xenograft formation assay. RESULTS: Low-expression of LINC00961 was detected in both OSCC tissues and cell lines. Through CCK-8 assay and flow cytometric analysis, we validated that up-regulated LINC00961 suppressed cell proliferation and promoted cell apoptosis in OSCC. Besides, over-expressed LINC00961 suppressed PI3K/AKT signaling pathways. In tumor xenograft formation assay, over-expressed LINC00961 inhibited tumor formation. CONCLUSIONS: Our research verified that LINC00961 functioned as a tumor suppressor in OSCC. Regulation of PI3K/AKT might be the underlying mechanism of the tumor suppressor role of LINC00961. The current study might bring a novel insight of biomarkers and therapeutic strategies in OSCC.
Asunto(s)
Regulación Neoplásica de la Expresión Génica , Neoplasias de la Boca/genética , Péptidos/metabolismo , Carcinoma de Células Escamosas de Cabeza y Cuello/genética , Animales , Apoptosis/genética , Línea Celular Tumoral , Proliferación Celular/genética , Progresión de la Enfermedad , Regulación hacia Abajo , Genes Supresores de Tumor , Humanos , Ratones , Mucosa Bucal/patología , Mucosa Bucal/cirugía , Neoplasias de la Boca/patología , Neoplasias de la Boca/cirugía , Fosfatidilinositol 3-Quinasas , Proteínas Proto-Oncogénicas c-akt , Transducción de Señal/genética , Carcinoma de Células Escamosas de Cabeza y Cuello/patología , Carcinoma de Células Escamosas de Cabeza y Cuello/cirugía , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
By means of indirect fluorescent antibody assay (IFA), Borrelia burgdorferi B31 strain was used for antigen, to detect the 2579 serum specimens from forest area in 9 counties of Fujian province, 47 cases in 8 counties were detected positive, positive rate was 1.82%. By epidemiological investigation, we found that peoples who infected with Lyme disease, 91.67% of them (22/24) have been bited by Ixodes, and 6 cases suffer typical clinical symptoms of Lyme disease, 9 cases have the similar or somewhat typical symptoms. We found Lyme disease could be cross-reaction to leptospirosis, in this paper the serum specimens which Lyme disease and leptospirosis all positive, we were not consider them to Lyme disease for time being, no matter the patients may be have suffer double infection. The indentification of the cross-reaction between Lyme disease and leptospirosis should be further studied.