Outer Membrane Vesicles: An Emerging Vaccine Platform.

Vaccine adjuvants are substances that improve the immune capacity of a recombinant vaccine to a great extent and have been in use since the early 1900s; they are primarily short-lived and initiate antigen activity, mainly an inflammatory response. With the developing technologies and innovation, early options such as alum were modified, yet the inorganic nature of major vaccine adjuvants caused several side effects. Outer membrane vesicles, which respond to the stressed environment, are small nano-sized particles secreted by gram-negative bacteria. The secretory nature of OMV gives us many benefits in terms of infection bioengineering. This article aims to provide a detailed overview of bacteria's outer membrane vesicles (OMV) and their potential usage as adjuvants in making OMV-based vaccines. The OMV adjuvant-based vaccines can be a great benefactor, and there are ongoing trials for formulating OMV adjuvant-based vaccines for SARS-CoV-2. This study emphasizes engineering the OMVs to develop better versions for safety purposes. This article will also provide a gist about the advantages and disadvantages of such vaccines, along with other aspects.

Reactive oxygen species: the root cause of nanoparticle-induced toxicity in Drosophila melanogaster.

Nanotechnology is a rapidly developing technology in the twenty-first century. Nanomaterials are extensively used in numerous industries including cosmetics, food, medicines, industries, agriculture, etc. Along with its wide application toxicity is also reported from studies of various model organisms including Drosophila. The toxicity reflects cytotoxicity, genotoxicity, and teratogenicity. The current study correlates the toxicity as a consequence of reactive oxygen species (ROS) generated owing to the presence of nanoparticles with the living cell. ROS mainly includes hydroxyl ions, peroxide ions, superoxide anions, singlet oxygen, and hypochlorous acids. An elevated level of ROS can damage the cells by various means. To protect the body from excess ROS, living cells possess a set of antioxidant enzymes which includes peroxidase, glutathione peroxidase, and catalase. If the antioxidant enzymes cannot nullify the elevated ROS level than DNA damage, cell damage, cytotoxicity, apoptosis, and uncontrolled cell regulations occur resulting in abnormal physiological and genotoxic conditions. Herewith, we are reporting various morphological and physiological defects caused after nanoparticle treatment as a function of redox imbalance.