Assessment of medicinal plants colonizing abundantly on metal-enriched fly ash deposits: phytoremediation prospective.

Heavy metal-enriched fly ash (FA) deposits are recognized as hazardous contaminated sites on the earth, which pollute our ecosystems. Consequently, the present investigation was carried out to explore the phytoremediation potential of naturally growing medicinal plants in the FA dumpsite. This present study chose two native medicinal plants i.e., Bacopa monnieri and Acmella oleracea found to be naturally colonizing abundantly on FA dumpsite to assess heavy metal accumulation. FA sample of B. monnieri thriving sites found to have metal content in order Mn (216.6)> Cr (39.27)> Zn (20.8)> Ni (16.1)> Cu (15.03)> Co (6.7)> Pb (5.43) whereas for A. oleracea FA dumpsites, the order of metal availability was Mn (750.3)> B (54.5)>Cr (37.2)>Zn (31.33)> Cu (18.7)> Ni (16.93)> Co (7.7)>Pb (4.23). In B. monnieri, higher concentrations of Cr and Mn were observed in the shoot in comparison to the root, indicative of its potential as a hyperaccumulator plant. Conversely, in A. oleracea, greater amounts of Pb were detected in the shoot relative to the root. Hence, it is recommended that B. monnieri and A. oleracea grow on such heavy metal-enriched substrates should be avoided for medicinal purposes; however, these plants can be used for phytoremediation purposes.

Fly ash phytoremediation through natural colonizer plant species is limited.Native colonizing plant species on fly ash has a pivotal role in phytoremediation.Naturally colonizing medicinal plants were dominant over the Fly ash dumpsites.Bacopa monnieri and Acmella oleracea have phytoremediation potential on fly ash.Indeed, fly ash-grown medicinal plants should not be used by local communities.

Diversity and activity of soil biota at a post-mining site highly contaminated with Zn and Cd are enhanced by metallicolous compared to non-metallicolous Arabidopsis halleri ecotypes.

Hyperaccumulators' ability to take up large quantities of harmful heavy metals from contaminated soils and store them in their foliage makes them promising organisms for bioremediation. Here we demonstrate that some ecotypes of the zinc hyperaccumulator Arabidopsis halleri are more suitable for bioremediation than others, because of their distinct influence on soil biota. In a field experiment, populations originating from metal-polluted and unpolluted soils were transplanted to a highly contaminated metalliferous site in Southern Poland. Effects of plant ecotypes on soil biota were assessed by measurements of feeding activity of soil fauna (bait-lamina test) and catabolic activity and functional diversity of soil bacteria underneath A. halleri plants (Biolog® ECO plates). Chemical soil properties, plant morphological parameters, and zinc concentration in shoots and roots were additionally evaluated. Higher soil fauna feeding activity and higher bacterial community functional diversity were found in soils affected by A. halleri plants originating from metallicolous compared to non-metallicolous ecotypes. Differences in community-level physiological profiles further evidenced changes in microbial communities in response to plant ecotype. These soil characteristics were positively correlated with plant size. No differences in zinc content in shoots and roots, zinc translocation ratio, and plant morphology were observed between metallicolous and non-metallicolous plants. Our results indicate strong associations between A. halleri ecotype and soil microbial community properties. In particular, the improvement of soil biological properties by metallicolous accessions should be further explored to optimize hyperaccumulator-based bioremediation technologies.

Investigating the recovery in ecosystem functions and multifunctionality after 10 years of natural revegetation on fly ash technosol.

Technogenic soil (technosol) developed from coal fly ash (FA) landfilling has been considered a critical environmental problem worldwide. Drought-tolerant plants often naturally grow on FA technosol. However, the impact of these natural revegetations on the recovery of multiple ecosystem functions (multifunctionality) remains largely unexplored and poorly understood. Here we assessed the response of multifunctionality, including nutrient cycling (i.e., carbon, nitrogen, and phosphorus), carbon storage, glomalin-related soil protein (GRSP), plant productivity, microbial biomass carbon (MBC), microbial processes (soil enzyme activities), and soil chemical properties (pH and electrical conductivity; EC) to FA technosol ten years' natural revegetation with different multipurpose species in Indo-Gangetic plain, and identified the key factors regulating ecosystem multifunctionality during reclamation. We evaluated four dominant revegetated species: Prosopis juliflora, Saccharum spontaneum, Ipomoea carnea, and Cynodon dactylon. We found that natural revegetation initiated the recovery of ecosystem multifunctionality on technosol, with greater recovery under higher biomass-producing species (P. juliflora and S. spontaneum) than lower biomass-producing ones (I. carnea and C. dactylon). The individual functions (11 of the total 16 variables) at higher functionality (70 % threshold) also exhibited this pattern among revegetated stands. Multivariate analyses revealed that most of the variables (except EC) significantly correlated with multifunctionality, indicating the capability of multifunctionality to consider the tradeoff between individual functions. We further performed structural equation modeling (SEM) to detect the effect of vegetation, pH, nutrients, and microbial activity (MBC and microbial processes) on ecosystem multifunctionality. Our SEM model predicted 98 % of the variation in multifunctionality and confirmed that the indirect effect of vegetation mediated by microbial activity is more important for multifunctionality than their direct effect. Collectively, our results demonstrate that FA technosol revegetation with high biomass-producing multipurpose species promotes ecosystem multifunctionality and emphasizes the significance of microbial activity in the recovery and maintenance of ecosystem attributes.

Seedling growth and physicochemical transformations of rice nursery soil under varying levels of coal fly ash and vermicompost amendment.

Fly ash is an inevitable by-product from the coal-fired power plants in many developing countries including India that needs safe, timely and productive disposal. The addition of fly ash alters physicochemical properties of soil and hence could be used as a soil conditioner or modifier along with the appropriate level of vermicompost to support plant growth. Several studies have focalized sole use of fly ash and vermicompost in agricultural production systems lacking information on combined application effects. This work was carried out at Chiplima in the district of Sambalpur, Odisha, India, to ascertain the best suited combination of native soil, fly ash and vermicompost (from farmyard manure) for rice nursery based on the changing physicochemical properties and seedling growth. The experiment consisting of 21 treatment combinations of soil, fly ash and vermicompost at 0%, 20%, 40%, 60%, 80% and 100% by weight was laid out in a factorial complete randomized design with three replications. Fly ash and vermicompost at moderate concentrations significantly ameliorated the physical properties, viz., porosity, bulk and particle densities, water holding capacity, infiltration rate and the capillary rise of water in rice nursery soil that ultimately resulted in vigorous rice seedlings at 40 DAS through beneficial soil biota as well as better root and shoot development. The porosity, water holding capacity and infiltration rate significantly increased with the addition of vermicompost while fly ash addition substantially reduced them. Fly ash and vermicompost in moderate quantities smothered soil chemical properties like electrical conductivity and organic carbon that increased the availability of N, P, K, B, S and Zn. The pH did not differ significantly due to treatment effects owing to a marginal difference in pH of the substrates, whereas electrical conductivity increased significantly with only marginal addition of fly ash to vermicompost. Considering the economic feasibility and environmental impacts, 40% soil + 20% fly ash + 40% vermicompost may be recommend to the farmers for wet rice nursery raising and also for remediating the coal fly ash in agricultural production system.

Effect of fly ash and vermicompost amendment on rhizospheric earthworm and nematode count and change in soil carbon pool of rice nursery.

Fly ash application to the soil at lower doses with organic substrates has been advocated by researchers due to its beneficial soil ameliorative properties. But its xenobiotic effects in presence of vermicompost have not yet been studied fully. The hypothesis of the present study was to ascertain the ameliorative effects of fly ash and vermicompost amendment on the soil nematode and earthworm count and change in the soil carbon pool of the rice nursery. The native soil, fly ash, and vermicompost at 0%, 20%, 40%, 60%, 80%, and 100% combinations (by weight) in triplicate were investigated under a factorial complete randomized design. The fly ash affected the earthworm survivability to an extent that the earthworms could not survive in fly ash of concentration greater than 20%. On the contrary, the concentration of vermicompost positively influenced the earthworm and nematode count in the rice rhizosphere. The population of nematodes viz. Rhabditis terricola and Dorylaimids in the rhizosphere of rice nursery was positively linked with the vermicompost concentration, while fly ash had antagonistic effects. The absence of nematodes and earthworms at a higher concentration of fly ash could be linked to the xenobiotic effects of fly ash. However, on mild addition of fly ash and vermicompost (20% each) to the native soil, the carbon stock increased positively to the maximum extent due to the larger surface area of fly ash and its xenobiotic effects limiting respirational carbon loss.

Understanding the Role of Litter Decomposition in Restoration of Fly Ash Ecosystem.

Plant species possess a huge potential in restoration of fly ash ecosystem. Litter deposition and its decomposition in the ash deposited sites are two important processes of the fly ash ecosystem. In order to identify the biological potential of a plant species to aid restoration of fly ash deposited sites, it is needed to assess leaf litter decomposition as well as nutrient release pattern. In the present investigation, we studied the leaf litter decomposition of the plant species (Leucaena leucocephala, Pithecellobium dolce and Prosopis juliflora) and mix plantation in the fly ash ecosystem. The litter bag experiment was conducted in the area of plantation on the fly ash deposited site during a period of 365 days. Percentage of C and N was higher in L. leucocephala > P. dolce >Mix Plantation > P. juliflora while C/N ratio was higher in P. juliflora >Mix Plantation > L. leucocephala > P. dolce. L. leucocephala and P. dolce showed relatively fast decomposition rates (k = 1.27, 1.17), respectively while mix plantation (k = 0.82) and P. juliflora (k = 0.73) exhibited relatively slower decomposition rates. Thus, we noted that the decomposition rate of L. leucocephala was greater than the other selected species. This shows that the species having faster decomposition rate and nutrient release could be a factual choice for rehabilitation of fly ash deposited sites.

Impact of pH on Pollutional Parameters of Textile Industry Wastewater with Use of Chlorella pyrenoidosa at Lab-Scale: A Green Approach.

The current study focused on the pollution remediation of textile industry wastewater by using Chlorella pyrenoidosa in two different physical forms: free algal biomass and immobilized algal biomass. The hypothesis behind the present study was to analyze the pollution reduction efficiency of immobilized algal biomass and free algal biomass on comparative scale on the basis of the adsorption process which is directly proportional with the surface area of the adsorbate. So, in this context the immobilized form of algae could enhance the pollution reduction efficiency due to availability of more surface area. So, the textile industry wastewater was treated by both free algal biomass and immobilized algal biomass and the major wastewater contributors like nitrate, phosphate, Biochemical Oxygen Demand (BOD) and Chemical Oxygen Demand (COD) were assessed before and after the treatment process. To conclude the optimum comparative results, the pH of wastewater was maintained constant, as it can capitalize or moderate the adsorption process (initial pH of was 8.2 ± 0.1, but it was maintained to 8). The contamination remediation was found to be effective with immobilized algal biomass (46.7% of nitrate, 59.4% of phosphate, 83.1% BOD and 83.0% of COD) than free algal biomass (43.2% of nitrate, 56.7% of phosphate, 71.4% of BOD and 78.0% COD).

Screening and Optimization of Zinc Removal Potential in Pseudomonas aeruginosa-HMR1 and its Plant Growth-Promoting Attributes.

The soil samples of old Zawar mine sites were sandy texture, basic, electric conductivity range from 16 to 59 dSm-1 with a high content of heavy metals of Zn, Pb, Cd, and Fe, indicating poor soil-health. Two bacterial isolates Pseudomonas aeruginosa HMR1 and P. aeruginosa HMR16 (GenBank-accession-number KJ191700 and KU174205, respectively), differed in the Phylogenetic tree based on 16S-rDNA sequences. HMR1 isolate showed the high potential of Plant growth-promoting attributes like IAA, Phosphate-solubilization, Exopolysaccharide production, and Proline activities at high concentration of Zn augmented nutrient media after 24 h, followed by HMR1 + HMR16 and HMR16. Both isolates were survived at 100 ppm Zn (w/v) concentration, followed by Pb, Cd, and Fe. Linear RL value from Langmuir and Freundlich isotherms revealed that the suitable condition of Zn adsorption by HMR1 was at pH8 with 40°C. The value of r2 from pseudo-second-order kinetics and Transmission-Electron-Microscopic analysis confirmed Zn adsorption by HMR1.

Potential and safe utilization of Fly ash as fertilizer for Pisum sativum L. Grown in phytoremediated and non-phytoremediated amendments.

The present study focuses on the possibility of applying fly ash to agricultural fields for enhancing the production of agricultural crops. In this study, Pisum sativum L. was grown from germination stage to maturation stage in phytoremediated and non-phytoremediated or raw fly ash-amended soil. All the morphological (height, biomass, number of leaf, and leaf size) and physiological parameters like, protein content, chlorophyll content, nitrate reductase activity, and peroxidase activity were monitored to understand the effects of fly ash or its usefulness for using it as a fertilizer for facilitating micronutrients. Major finding of this study is that 40% (w/w) of non-phytoremediated fly ash amendment could be used for field application. Percentage increase of toxic metals in below ground organs was 6% for Cd, 6% for Cr, 5% for Cu, 15% for Mn, and 7% for Pb when compared with the control. In the non-phytoremediated fly ash-amended set, heavy metals and metalloids were present in the grains only at higher amendments T3 (60%) and T4 (80%). However, except Cd, all the metals were below the permissible limits suggested by the WHO. Phytoremediated fly ash could be used as a fertilizer up to 100% for the cultivation of pea plant as metals concentrations were found either below detection limit or below the WHO permissible limit.

Ecological restoration of coal fly ash-dumped area through bamboo plantation.

The present study entails the phytoremediation potential of different bamboo species on 5-year-old FA-dumped site near Koradi thermal power plant of Nagpur, Maharashtra, India. The selected FA-dumped site was treated with farmyard manure, press mud, and bio fertilizer followed by plantation of six promising species of bamboo namely Bambusa balcooa Roxb., Dendrocalamus stocksii (Munro.) M. Kumar, Remesh and Unnikrishnan, Bambusa bambos (L.) Voss, Bambusa wamin E.G. Camus, Bambusa vulgaris var. striata (Lodd. ex Lindl.) Gamble, and Bambusa vulgaris var. vittata Riviere and Riviere. The experimental results indicated that the organic input in the FA-dumped site nourished the soil by improving its physico-chemical, and biological characteristics. The results revealed the contamination of the site with different trace elements in varied quantity including Cr (89.29 mg kg-1), Zn (84.77 mg kg-1), Ni (28.84 mg kg-1), Cu (22.91 mg kg-1), Li (19.65 mg kg-1), Pb (13.47 mg kg-1), and Cd (2.35 mg kg-1). A drastic reduction in concentration of heavy metals in FA was observed after 1 year of bamboo plantation as compared to the initial condition. The results showed that bamboo species are good excluders of Ba, Co, Cr, Li, Ni, Mn, and Zn, whereas they are good accumulators of Cd, Pb, and Cu. The values of biochemical parameters, such as pH, total chlorophyll, ascorbic acid (AA), and relative water content of all the bamboo leaves ranged from 5.11-5.70, 1.56-6.33 mg g-1, 0.16-0.19 mg g-1, and 60.23-76.68%, respectively. It is thereby concluded that the bamboo plantation with biofertilizers and organic amendments may indicate adaptive response to environmental pollution on FA-dumped site.

Metal remediation potential of naturally occurring plants growing on barren fly ash dumps.

The present study aimed to elucidate the remediation potential of visibly dominant, naturally growing plants obtained from an early colonized fly ash dump near a coal-based thermal power station. The vegetation comprised of grasses like Saccharum spontaneum L., Cynodon dactylon (L.) Pers., herbs such as Tephrosia purpurea (L.) Pers., Sida rhombifolia L., Dysphania ambrosioides (L.) Mosyakin & Clemants, Chromolaena odorata (L.) King & H.E. Robins along with tree saplings Butea monosperma (Lam.) Taub. The growth of the vegetation improved the N and P content of the ash. Average metal concentrations (mg kg-1) in the ash samples and plants were in order Mn (345.1) > Zn (63.7) > Ni (29.3) > Cu (16.8) > Cr (9.9) > Pb (1.7) > Cd (0.41) and Cr (58.58) > Zn (52.74) > Mn (39.09) > Cu (10.71) > Ni (7.45) > Pb (5.52) > Cd (0.14), respectively. The plants showed fly ash dump phytostabilization potential and accumulated Cr (80.19-178.11 mg kg-1) above maximum allowable concentrations for plant tissues. Positive correlations were also obtained for metal concentration in plant roots versus fly ash. Saccharum spontaneum showed highest biomass and is the most efficient plant which can be used for the restoration of ash dumps.

Phytoremediation ability of naturally growing plant species on the electroplating wastewater-contaminated site.

The presence of heavy metal in soil and water resources has serious impact on human health. The study was designed to examine the phytoremediation ability of plant species that are growing naturally on the Zn-contaminated site. For the study, six plant species and their rhizospheric soil as well as non-rhizospheric soil samples were collected from different parts of the industrial sites for chemical and biological characterization. Visual observations and highest importance value index (IVI) through biodiversity study revealed potential plants as effective ecological tools for the restoration of the contaminated site. Among the plants, almost all were the most efficient in accumulating Fe, Mn, Cu and Zn in its shoots and roots, while Cynodon dactylon, Chloris virgata and Desmostachya bipinnata were found to be stabilizing Cr, Pb and Cd (bioconcentration factor in root = 7.95, 6.28 and 1.98 as well as translocation factor = 0.48, 0.46 and 0.78), respectively. Thus, the results of this study showed that the naturally growing plant species have phytoremediation potential to remediate the electroplating wastewater-contaminated site. These plant species are successful phytoremediators with their efficient metal stabilizing and well-evolved tolerance to heavy metal toxicity.

Pteridophytes in phytoremediation.

Soil contamination by heavy metals and metalloids is a serious problem which needs to be addressed. There are several methods for removal of contaminants, but they are costly, while the method of phytoremediation is eco-friendly and cost-effective. Pteridophytes have been found to remediate heavy metal-contaminated soil. Pteridophytes are non-flowering plant that reproduces by spores. Pteris vittata has been reported as the first fern plant to hyperaccumulate arsenic. The Pteris species belongs to the order Pteridales. Other ferns that are known phytoremediators are, for example, Nephrolepis cordifolia and Hypolepis muelleri (identified as phytostabilisers of Cu, Pb, Zn and Ni); similarly Pteris umbrosa and Pteris cretica accumulate arsenic in leaves. So, pteridophytes have a number of species that accumulate contaminants. Many of them have been identified, while various other are being explored. The present review article describes the phytoremediation potential of pteridophytes plants and suggests as a potential asset for phytoremediation programs.

Assessment of Ziziphus mauritiana grown on fly ash dumps: Prospects for phytoremediation but concerns with the use of edible fruit.

A field study was carried out on fly ash (FA) dumps of Panki Thermal Power Station to assess the phytoaccumulation of elements in various plant parts of edible fruit tree Ziziphus mauritiana. Of the twelve analyzed elements, the highest concentration was found for Fe followed by Mn > Se > Zn > Mo > Cu > Cr > Pb > Cd >Ni > As > Co in rhizospheric substrate of Z. mauritiana grown on FA dumps. Metal accumulation, bioconcentration factor, and translocation factor for each metal were calculated in various parts of the edible fruit tree. Significant variations of metal accumulations were observed among various plant parts. Accumulation of toxic elements was higher in roots, and it gradually declined toward the aerial parts of the plant corresponding to its distance from the ground. The concentration of some elements in fruit tree was found to be above prescribed limits in edible parts. Therefore, the present study suggested that additional care should be undertaken, if edible fruit trees are considered for phytoremediation or afforestation programs of FA dumps.

Phytoremediation efficiency of Eichhornia crassipes in fly ash pond.

The present study was focused on field research to examine the phytoremediation potential of naturally grown Eichhornia crassipes in fly ash (FA) pond. Field results indicate the efficiency of E. crassipes for remediation of heavy metals from FA pond. The bioconcentration factor trend was Cr (3.75) > Cu (2.62) > Cd (1.05), and Cu (1.35) in root and stem, respectively. The survival and abundance growth of E. crassipes in the circumstance of heavy metal enriched FA pond is another highlight of the present research that reveals its toxitolerant characteristics. Thus, this lesson on phytoremediation proved that E. crassipes is a potential accumulator of Cu, Cr, and Cd from FA ponds and is a promising species for FA pond's remediation globally.

Phytodiversity on fly ash deposits: evaluation of naturally colonized species for sustainable phytorestoration.

Proliferation of fly ash (FA) deposits and its toxicity have become a global concern, which contaminate the ecosystems of our Earth. In this regard, identification of potential plant species for FA deposits' restoration is the main concern. Keeping this view in mind, the present study was conducted to identify potential plant species naturally growing on FA deposits for the restoration purposes. Six intensive surveys were made during 2010-2014 to collect naturally growing plant species during different seasons from two FA deposits in Unchahar of Raebareli district, Uttar Pradesh, India. The plant species having potential for FA deposits' restoration were identified on the basis of their ecological importance, dominance at the study sites and socio-economic importance for rural livelihoods. Typha latifolia L., Cynodon dactylon (L.) Pers., Saccharum spontaneum L., Saccharum bengalense Retz. (syn. Saccharum munja), Prosopis juliflora (Sw.) DC., Ipomoea carnea Jacq. and Acacia nelotica L. are identified as potential plant species for FA deposits' restoration. Furthermore, the characteristics of naturally colonized species can be used for the phytorestoration during a revegetation plan of new FA deposits for multiple benefits.

Fly ash application in nutrient poor agriculture soils: impact on methanotrophs population dynamics and paddy yields.

There are reports that the application of fly ash, compost and press mud or a combination thereof, improves plant growth, soil microbial communities etc. Also, fly ash in combination with farmyard manure or other organic amendments improves soil physico-chemical characteristics, rice yield and microbial processes in paddy fields. However, the knowledge about the impact of fly ash inputs alone or in combination with other organic amendments on soil methanotrophs number in paddy soils is almost lacking. We hypothesized that fly ash application at lower doses in paddy agriculture soil could be a potential amendment to elevate the paddy yields and methanotrophs number. Here we demonstrate the impact of fly ash and press mud inputs on number of methanotrophs, antioxidants, antioxidative enzymatic activities and paddy yields at agriculture farm. The impact of amendments was significant for methanotrophs number, heavy metal concentration, antioxidant contents, antioxidant enzymatic activities and paddy yields. A negative correlation was existed between higher doses of fly ash-treatments and methanotrophs number (R(2)=0.833). The content of antioxidants and enzymatic activities in leaves of higher doses fly ash-treated rice plants increased in response to stresses due to heavy metal toxicity, which was negatively correlated with rice grain yield (R(2)=0.944) and paddy straw yield (R(2)=0.934). A positive correlation was noted between heavy metals concentrations and different antioxidant and enzymatic activities across different fly ash treated plots.The data of this study indicate that heavy metal toxicity of fly ash may cause oxidative stress in the paddy crop and the antioxidants and related enzymes could play a defensive role against phytotoxic damages. We concluded that fly ash at lower doses with press mud seems to offer the potential amendments to improving soil methanotrophs population and paddy crop yields for the nutrient poor agriculture soils.

Phytoremediation of heavy metals from fly ash pond by Azolla caroliniana.

Abundance of naturally growing Azolla caroliniana (water fern) on the surface of metal enriched fly ash (FA) pond reflects its toxitolerant characteristics. Results indicate the efficiency of A. caroliniana for phytoremediation of FA pond because of its higher bioconcentration factor. The metal concentration ranged from 175 to 538 and 86 to 753mgkg(-1) in roots and fronds, respectively. Bioconcentration factor (BCF) values of all metals in root and frond ranged from 1.7 to18.6 and 1.8 to 11.0, respectively, which were greater than one and indicates the metal accumulation potential of A. caroliniana. Translocation factor (TF) ranged from 0.37 to 1.4 for various heavy metals. The field result proved that A. caroliniana is a potential accumulator for the examined heavy metals and can be used for phytoremediation of FA pond.

Documentation and determination of consensus about phytotherapeutic veterinary practices among the Tharu tribal community of Uttar Pradesh, India.

PURPOSE: The aim of the present investigation was to document the phytotherapeutic knowledge and veterinary healthcare management practices among the Tharu tribal community of Uttar Pradesh, India and to determine the consensus of such practices, in order to evaluate the potential for new veterinary drugs of herbal origin. METHODS: This study was conducted in 2000-2004 using semistructured, open-ended questionnaires, informal interviews and group discussions with farmers engaged in animal husbandry. RESULTS: In the present study, 59 phytotherapeutic practices using 48 plant species were documented for management of 18 types of healthcare problems of domesticated animals. Crude drug formulations keep the animal healthy, increase lactation, and reduce estrus interval and puberty period to make them economically more important. There was great agreement among informants regarding phytotherapeutic uses of medicinal plants with factor of informants' consensus (F(IC)) value ranging from 0.84 to 1, with an average value of 0.94. CONCLUSION: Study reveals that there is great agreement among informants for the usages of Azadirachta indica A Juss, Bombax ceiba L, Bambusa arundinacea (Retz) Willd, Corianderum sativum L, Cuscuta reflexa Roxb, Datura metal L, Nelumbo nucifera Gaertn, and Parthenium hysterophorus L. These species may be used for the development of new, cheep, effective, and eco-friendly herbal formulations for veterinary healthcare management. Further investigation of these herbal formulations for veterinary healthcare management will require safety and efficacy testing. There is an urgent need to formulate suitable conservation strategies for wildly growing phytotherapeutics to overcome their depletion from natural resources and to make these practices more eco-friendly.