Detection and modeling of Staphylococcus aureus and fecal bacteria in Hawaiian coastal waters and sands.

Microbial pollution of recreational waters leads to millions of skin, respiratory, and gastrointestinal illnesses globally. Fecal indicator bacteria (FIB) are monitored to assess recreational waters but may not reflect the presence of Staphylococcus aureus, a global leader in bacterial fatalities. Since many community-acquired S. aureus skin infections are associated with high recreational water usage, this study measured and modeled S. aureus, methicillin-resistant S. aureus (MRSA), and FIB (Enterococcus spp., Clostridium perfringens) concentrations in seawater and sand at six beaches in Hilo, Hawai'i, USA, over 37 sample dates from July 2016 to February 2019 using culturing techniques. Generalized linear models predicted bacterial concentrations with physicochemical and environmental data. Beach visitors were also surveyed on their preferred activities. S. aureus and FIB concentrations were roughly 6-78 times higher at beaches with freshwater discharge than at those without. Seawater concentrations of Enterococcus spp. were positively associated with MRSA but not S. aureus. Elevated S. aureus was associated with lower tidal heights, higher freshwater discharge, onsite sewage disposal system density, and turbidity. Regular monitoring of beaches with freshwater input, utilizing real-time water quality measurements with robust modeling techniques, and raising awareness among recreational water users may mitigate exposure to S. aureus, MRSA, and FIB. PRACTITIONER POINTS: Staphylococcus aureus and fecal bacteria concentrations were higher in seawater and sand at beaches with freshwater discharge. In seawater, Enterococcus spp. positively correlated with MRSA, but not S. aureus. Freshwater discharge, OSDS density, water turbidity, and tides significantly predicted bacterial concentrations in seawater and sand. Predictive bacterial models based upon physicochemical and environmental data developed in this study are readily available for user-friendly application.

Detection and impact of sewage pollution on South Kohala's coral reefs, Hawai'i.

Sewage pollution from on-site sewage disposal systems and injection wells is impacting coral reefs worldwide. Our study documented the presence and impact of sewage on South Kohala's coral reefs, on Hawai'i Island, through benthic water quality and macroalgal sampling (fecal indicator bacteria, nutrients, δ15N macroalgal tissue), NO3- stable isotope mixing models, water motion measurements, and coral reef surveys. Sewage pollution was moderate on the offshore reef from benthic seeps, and water motion mixed and diluted it across the benthos. These conditions likely contribute to the dominance of turf algae cover, and the severity and prevalence of growth anomalies and algal overgrowth on corals. Use of multiple indicators and studying water motion was necessary to assess sewage pollution and identify environmental drivers associated with impaired coral health conditions. Methods used in this study can be utilized by natural resource managers to identify and reduce anthropogenic stressors to coral reefs.

Spatial distribution and sources of nutrients at two coastal developments in South Kohala, Hawai'i.

Nutrient sources to coastal waters with coral reefs are not well-characterized. This study documented spatial distributions of nutrients within coastal waters along two developments with coral reefs, and identified nutrient sources through nutrient mixing plots, δ15N measurements in macroalgal tissue, and NO3- stable isotope mixing models. Nutrients decreased from fresh groundwaters to offshore waters, with some surface waters higher in concentrations than benthic ones. Conservative and non-conservative mixing between fresh and ocean waters occurred, the latter suggestive of local nutrient sources and biological removal. δ15N in macroalgal tissue and NO3- concurred that fresh groundwater, ocean water, and fertilizers were dominant nutrient sources. Benthic salinity and NO3- + NO2- concentrations illustrated that submarine groundwater discharge delivered nutrients to reefs in pulses ranging from minutes to days. Information generated from this study is imperative for developing management actions to improve water quality and make coral reefs more resilient to stressors.