RESUMEN
BACKGROUND: The peripheral ossifying fibroma (POF) represents one of the most common lesions of the periodontal tissues that may originate from the gingival soft tissues, the periosteum, or the periodontal ligament. AIM: To investigate the immunohistochemical expression of runt-related transcription factor 2 (Runx-2), bone morphogenetic protein-2 (BMP-2), and cementum attachment protein (CAP) in oxytalan-positive POF, to establish the use of POF as an in vivo model for the study of the periodontal ligament. MATERIALS AND METHODS: Thirty tumors that presented clinical and histologic features of POF, as well as oxytalan fibers, were included in the study. Immunohistochemical expression of Runx-2, BMP-2, and CAP was evaluated by light microscopy. RESULTS: Runx-2, BMP-2, and CAP were abundantly expressed by POFs; 22 of 30 tumors expressed positive staining for Runx-2, twenty-six tumors for BMP-2, and twenty-five tumors for CAP. The expression of Runx-2 was abundant in POFs where bone was histologically present (P = 0.04) and of BMP-2 in POFs where dystrophic calcifications were present (P = 0.03). CONCLUSION: It is suggested that oxytalan-positive POFs, purportedly originating from the periodontal ligament, express molecules that are specific to bone and cementum (Runx-2, BMP-2), or cementum only (CAP). Thus, the cell populations present in the lesion belong to the mineralized-tissue-forming cell lineages, the cementoblastic or osteoblastic lineage.
Asunto(s)
Proteína Morfogenética Ósea 2/biosíntesis , Neoplasias Óseas/metabolismo , Subunidad alfa 1 del Factor de Unión al Sitio Principal/biosíntesis , Proteínas de la Matriz Extracelular/biosíntesis , Fibroma Osificante/metabolismo , Neoplasias Gingivales/metabolismo , Proteínas Tirosina Fosfatasas/biosíntesis , Proteína Morfogenética Ósea 2/genética , Neoplasias Óseas/patología , Calcificación Fisiológica , Diferenciación Celular/fisiología , Subunidad alfa 1 del Factor de Unión al Sitio Principal/genética , Subunidad alfa 1 del Factor de Unión al Sitio Principal/metabolismo , Cemento Dental/metabolismo , Femenino , Fibroma Osificante/patología , Encía/metabolismo , Encía/patología , Humanos , Inmunohistoquímica , Masculino , Osteoblastos/metabolismo , Ligamento Periodontal/metabolismo , Ligamento Periodontal/patología , Proteínas Tirosina Fosfatasas/genética , Estudios RetrospectivosRESUMEN
BACKGROUND: The pyogenic granuloma (PG) is a common localized hyperplastic lesion of the oral cavity. The purpose of the present study was to investigate the immunohistochemical expression of endothelial nitric oxide synthases (eNOS) and CD105/endoglin in oral PGs, to evaluate their involvement in the angiogenetic pathways of the lesion. MATERIALS AND METHODS: Ninety-three PGs were included in the study. Sixteen tumors were further sub-classified as pregnancy tumors (PT) and seventeen as pyogenic granulomas with fibrosis (PGFM). Immunohistochemical expression of eNOS and CD105/endoglin was quantified by computerized image analysis with a semi-automated system. Percentage of staining and number of objects (positive vessels) were recorded for each case. RESULTS: Intense eNOS expression was seen in 92 of 93 lesions. A statistically significant association was found between eNOS percentage of staining/eNOS positive vascular spaces (objects) and age of the patients (9% increase per decade of life). Approximately 40% less eNOS positive objects were recorded in PGFM compared with PGs. Intense membranous CD105/endoglin expression was seen in all cases. The percentage of CD105/endoglin staining was statistically increased in PGs compared with PT. Approximately 40% less CD105/endoglin objects were found in PGFM compared with PGs; 56% more CD105/endoglin objects were found in tongue lesions, compared with gingival lesions. There was no statistically significant correlation considering percentage of staining and number of objects between CD105/endoglin and eNOS. CONCLUSIONS: It is suggested that eNOS and CD105/endoglin are involved in the angiogenetic pathways of PG.