Comparative assessment of metazoans infestation of Nile tilapia (Oreochromis niloticus) (L.) (Perciformes: Cichlidae) in floating cages and ponds from Chiapas, Mexico.

Nile tilapia is the most commercially important fish in Chiapas as well as in other parts of the world. An understanding of parasite infection dynamics in tilapia may assist in applying proper prophylactic measures for reducing the loss of fish caused by parasitic diseases. Different environments and culture systems may imply different infection dynamics; therefore, the present study identified and compared the infection parameters (prevalence, mean abundance, and intensity) of parasites of Oreochromis niloticus cultured in floating cages and ponds. A total of 18 metazoan parasite taxa were recovered from gills, skin, fins and intestines of 310 specimens from floating cages and ponds. Fourteen species of parasites were found in floating cages: 8 monogenean species, 1 nematode, 1 digenea, 1 crustacea, 3 protozoans. In ponds, 16 parasite taxa were reported: 11 were monogeneans species, 4 protozoans and 1 crustacean. In both systems, monogeneans (Cichlidogyrus sclerosus, C. tilapiae, Gyrodactylus cichlidarum) and protozoans (Trichodina compacta) were most prevalent. Tilapia in ponds sustained higher parasitic infections than those in floating cages (p < 0.05). The Canonical Correspondence Analysis showed two groups: the first grouped the ponds, associated with high values of nitrite, nitrate, ammonia, conductivity, temperature, and the abundance of the ectoparasites C. halli, C. dossoui, Scutogyrus longicornis, C. sclerosus and T. compacta. The second group grouped the cages, associated with Clinostomum marginatum, Apiosoma piscicola, Lernea sp., and Contracaecum sp. and a high dissolved oxygen concentration. Our findings suggest that monitoring programs are required to improve the sanitary conditions of tilapia cultures in Chiapas.

A systematic review and meta-analysis of the relationship between farm management, water quality and pathogen outbreaks in tilapia culture.

A systematic review and meta-analysis of on farm management and water quality as risk factors triggering pathogen outbreaks in tilapia culture is presented. A total of 121 papers from 28 countries, 37 devoted to management and 84 to water quality associated with the presence of pathogens in tilapia culture were analysed. Most of research has been made on ponds, focusing on bacteria and metazoans. Meta-analysis reveals the proportion of research showing statistical inferences between management (34%), water quality (38% for bacteria and 16.2% for metazoans) and pathogen outbreaks. The most cited parameters related to pathogens outbreaks were temperature (>30°C), dissolved oxygen (<5.0 mg/L), pH (<8.0) and ammonia (1.0 >mg/L) 65, 38, 32 and 34 papers, respectively. However, statistical inference was <20%. Meta-analysis reveals tendencies between high temperatures (30-35°C), low dissolved oxygen (<5 mg/L) and high NH3 (1-5 mg/L) with pathogen outbreaks. Despite the knowledge about the relationship between management and water quality as factors for pathogen effects, most information is descriptive and empirical. Future research on tilapia culture outbreaks should be focused on the effects of multiple stressors affecting tilapia pathogen outbreaks, thereby generating strategies to prevent diseases and financial losses.

Negative effect of ectoparasite burdens on the condition factor from farmed tilapia Oreochromis niloticus in the Yucatan, Mexico.

Nile tilapia (Oreochromis niloticus Linnaeus, 1758) is one of the most important aquaculture species in the world, and, when introduced, the ectoparasites of Nile tilapia have followed. Currently, farmers worldwide consider these ectoparasites harmless, but intensities can reach up to 1000 individuals per fish in tropical regions. In this cross-sectional study, we used the condition factor to estimate the potential effects of low (45 ± 31 ectoparasites per fish) and high (295 ± 191) ectoparasitic burdens across 28 tilapia farms and included the analysis of the effects of 44 management and environmental variable from the farms. A stepwise procedure in a multiple linear regression analysis retained the variables that explained the most variance, which was the ectoparasitic burden (57 %). We found significantly higher values of the condition factor in Nile tilapia with low ectoparasitic burden than in those with high ectoparasitic burden. Additionally, Nile tilapia with a high ectoparasitic burden weighed less than half than those with a low burden (102 ± 105 g versus 230 ± 128 g, respectively). We also found a significant non-linear, negative relationship between the ectoparasitic burden and the relative condition factor values per fish, which was most likely due to an increase in gill mucus caused by the ectoparasitic burden that depleted energy in the Nile tilapia. The economic consequence of the ectoparasitic burden translated roughly into a loss up to 12-15 % in profit margin per ton of fish, based on the price of Nile tilapia in the Mexican market.

Geographical distribution of protozoan and metazoan parasites of farmed Nile tilapia Oreochromis niloticus (L.) (Perciformes: Cichlidae) in Yucatán, México.

BACKGROUND: In Yucatán State, southern México, as in many other parts of the world where tilapia has been introduced for aquaculture, the deficient application of management measures has led to the establishment of non-native parasites. The aims of this study were to describe the geographical distribution of protozoan and helminth parasites of farmed Nile tilapia Oreochromis niloticus (L.) throughout the Yucatán and to examine the potential statistical associations of the prevalence and mean abundance of these parasites with management and environmental variables. METHODS: All 29 Nile tilapia farms currently operating in Yucatán were surveyed. Maps were created to describe the geographical location of the parasites infecting Nile tilapia at each farm. We evaluated the statistical associations of management and environmental variables with the mean abundance values of each parasite species using a multivariate redundancy analysis (RDA) and generalized additive models (GAM). We also used Ripley's K to determine whether there were significant clusters of the mean abundance of particular parasite species in specific regions of the Yucatán State. RESULTS: A total of 580 O. niloticus were examined, and 11 species of parasites were recorded. Cichlidogyrus sclerosus was the most frequent and abundant parasite at all 29 farms, whereas Gyrodactylus cichlidarum was found in 26 of the 29 farms. The RDA showed that the most important predictors were the concentration of nitrites and ammonium and the water exchange rate. The GAM showed the significant effect of the tank capacity, no use of quarantine area and no use of prophylactic treatments on the mean abundance of G. cichlidarum. The geographical distribution patterns of the mean abundance of most parasite species exhibited clustering near to the coast of Yucatán. CONCLUSION: Two groups of farms were distinguished: (i) farms with medium to high technology, where the most frequent and abundant parasite was G. cichlidarum, and (ii) farms with low technology, where the most frequent and abundant parasite was C. sclerosus. Good biosecurity practices such as the use of quarantine and prophylactic treatments prior to the introduction of infected Nile tilapia to the farms are recommended to avoid the establishment of parasites such as G. cichlidarum in farms.

The introduced Asian parasitic copepod Neoergasilus japonicus (Harada) (Cyclopoida: Ergasilidae) from endangered cichlid teleosts in Mexico.

The cyclopoid copepod Neoergasilus japonicus ( Harada, 1930 ) is recorded from three endangered or threatened fish species from southeast Mexico: the tailbar cichlid Vieja hartwegi (Taylor and Miller, 1980); the Angostura cichlid V. breidohri (Werner and Stawikowski, 1987); and the sieve cichlid C. grammodes (Taylor and Miller, 1980). This ectoparasitic copepod is considered, together with most other members of Neoergasilus, an Eastern Asian form. N. japonicus is one of the most widespread parasitic Asian copepods, as it has rapidly invaded Europe and North America, including Mexico. We estimated the prevalence, mean abundance, and intensity of infection of N. japonicus in these cichlid teleosts; our data agree with previous works stating the high prevalence of this ectoparasite. This copepod has a wide range of hosts among freshwater fish taxa, but this is only the second published report from cichlids in the Neotropical region. The three cichlids surveyed, V. hartwegi, V. breidohri, and C. grammodes, are new hosts of this copepod. Its occurrence in Mexico is attributed to different events of introduction by human agency. This is the southernmost record of N. japonicus in continental America. It is a matter of concern that this copepod is parasitizing endangered or threatened endemic cichlids in the Neotropical region. Because its high infective efficiency and ability to shift hosts, this Asian parasite is expected to spread farther southwards into Central and South America.